1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	T	E	L	A	P	S	L	V	E	L	A	R	R	F	G	I	A	T	E	Y	T	D	W	T	G	R	Q	V	L	V	S	E	A	T	L	V	A	A	L	A	A	L	G	V	P	A	Q	T	E	Q	Q	R	N	D	A	L	A	A	Q
Secondary structure
SS confidence
Disorder		?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		L	R	S	Y	W	A	R	P	L	P	A	T	I	V	M	R	A	G	E	Q	T	Q	F	R	V	H	V	T	D	G	A	P	A	D	V	W	L	Q	L	E	D	G	T	T	R	A	E	V	V	Q	V	D	N	F	T	P	P	F	D	L
Secondary structure
SS confidence
Disorder																																																							?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		D	G	R	W	I	G	E	A	S	F	V	L	P	A	D	L	P	L	G	Y	H	R	V	N	L	R	S	G	D	S	Q	A	S	A	A	V	V	V	T	P	D	W	L	G	L	P	D	K	L	A	G	R	R	A	W	G	L	A	V	Q
Secondary structure
SS confidence
Disorder																													?	?	?												?				?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		L	Y	S	V	R	S	R	Q	S	W	G	I	G	D	L	T	D	L	A	N	L	A	L	W	S	A	S	A	H	G	A	G	Y	V	L	V	N	P	L	H	A	A	T	L	P	G	P	A	G	R	S	K	P	I	E	P	S	P	Y	L
Secondary structure
SS confidence
Disorder																																													?	?	?	?	?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		P	T	S	R	R	F	V	N	P	L	Y	L	R	V	E	A	I	P	E	L	V	D	L	P	K	R	G	R	V	Q	R	L	R	T	N	V	Q	Q	H	A	D	Q	L	D	T	I	D	R	D	S	A	W	A	A	K	R	A	A	L	K
Secondary structure
SS confidence
Disorder																														?	?				?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		L	V	H	R	V	P	R	S	A	G	R	E	L	A	Y	A	A	F	R	T	R	E	G	R	A	L	D	D	F	A	T	W	C	A	L	A	E	T	Y	G	D	D	W	H	R	W	P	K	S	L	R	H	P	D	A	S	G	V	A	D
Secondary structure
SS confidence
Disorder								?	?	?	?	?										?																							?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		F	V	D	K	H	A	D	A	V	D	F	H	R	W	L	Q	W	Q	L	D	E	Q	L	A	S	A	Q	S	Q	A	L	R	A	G	M	S	L	G	I	M	A	D	L	A	V	G	V	H	P	N	G	A	D	A	W	A	L	Q	D	V
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		L	A	Q	G	V	T	A	G	A	P	P	D	E	F	N	Q	L	G	Q	D	W	S	Q	P	P	W	R	P	D	R	L	A	E	Q	E	Y	R	P	F	R	A	L	I	Q	A	A	L	R	H	A	G	A	V	R	I	D	H	I	I	G
Secondary structure
SS confidence
Disorder																																			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		L	F	R	L	W	W	I	P	D	G	A	P	P	T	Q	G	T	Y	V	R	Y	D	H	D	A	M	I	G	I	V	A	L	E	A	H	R	A	G	A	V	V	V	G	E	D	L	G	T	V	E	P	W	V	R	D	Y	L	L	L	R
Secondary structure
SS confidence
Disorder																																					?
Disorder confidence
		.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560	.	.	.	.	.	.	.	.	.	570	.	.	.	.	.	.	.	.	.	580	.	.	.	.	.	.	.	.	.	590	.	.	.	.	.	.	.	.	.	600
Sequence		G	L	L	G	T	S	I	L	W	F	E	Q	D	R	D	C	G	P	A	G	T	P	L	P	A	E	R	W	R	E	Y	C	L	S	S	V	T	T	H	D	L	P	P	T	A	G	Y	L	A	G	D	Q	V	R	L	R	E	S	L	G
Secondary structure
SS confidence
Disorder															?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	610	.	.	.	.	.	.	.	.	.	620	.	.	.	.	.	.	.	.	.	630	.	.	.	.	.	.	.	.	.	640	.	.	.	.	.	.	.	.	.	650	.	.	.	.	.	.	.	.	.	660
Sequence		L	L	T	N	P	V	E	A	E	L	E	S	A	R	A	D	R	A	A	W	M	A	E	L	R	R	V	G	L	L	A	D	G	A	E	P	D	S	E	E	A	V	L	A	L	Y	R	Y	L	G	R	T	P	S	R	L	L	A	V	A
Secondary structure
SS confidence
Disorder							?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	670	.	.	.	.	.	.	.	.	.	680	.	.	.	.	.	.	.	.	.	690	.	.	.	.	.	.	.	.	.	700	.	.	.	.	.	.	.	.	.	710	.	.	.	.	.	.	.	.	.	720
Sequence		L	T	D	A	V	G	D	R	R	T	Q	N	Q	P	G	T	T	D	E	Y	P	N	W	R	V	P	L	T	G	P	D	G	Q	P	M	L	L	E	D	I	F	T	D	R	R	A	A	T	L	A	E	A	V	R	A	A	T	T	S	P
Secondary structure
SS confidence
Disorder										?	?																				?		?																							?	?	?	?	?	?
Disorder confidence
		.	.	.	.
Sequence		M	S	C	W
Secondary structure
SS confidence
Disorder		?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 10%) Alpha helix ( 40%) Beta strand ( 13%)

Rank Aligned region

PDB 5b68 chain A Region: 6 - 716 Aligned: 686 Modelled: 697 Confidence: 100.0% Identity: 47% PDB header:transferase Chain: A: PDB Molecule:4-alpha-glucanotransferase; PDBTitle: crystal structure of apo amylomaltase from corynebacterium glutamicum Phyre2 PDB 4s3r chain A Region: 5 - 717 Aligned: 657 Modelled: 670 Confidence: 100.0% Identity: 33% PDB header:transferase Chain: A: PDB Molecule:4-alpha-glucanotransferase; PDBTitle: amylomaltase malq from escherichia coli in complex with the pseudo-2 heptasaccharide acarviosine-glucose-acarbose Phyre2 PDB 1x1n chain A domain 1 Region: 164 - 715 Aligned: 489 Modelled: 506 Confidence: 100.0% Identity: 22% Fold: TIM beta/alpha-barrel Superfamily: (Trans)glycosidases Family: Amylase, catalytic domain Phyre2 PDB 1esw chain A Region: 169 - 700 Aligned: 474 Modelled: 501 Confidence: 100.0% Identity: 27% Fold: TIM beta/alpha-barrel Superfamily: (Trans)glycosidases Family: Amylase, catalytic domain Phyre2 PDB 1tz7 chain A domain 1 Region: 173 - 712 Aligned: 469 Modelled: 501 Confidence: 100.0% Identity: 23% Fold: TIM beta/alpha-barrel Superfamily: (Trans)glycosidases Family: Amylase, catalytic domain Phyre2 PDB 4e2o chain A Region: 186 - 602 Aligned: 255 Modelled: 270 Confidence: 98.4% Identity: 15% PDB header:hydrolase/hydrolase inhibitor Chain: A: PDB Molecule:alpha-amylase; PDBTitle: crystal structure of alpha-amylase from geobacillus thermoleovorans,2 gta, complexed with acarbose Phyre2 PDB 3zt5 chain D Region: 186 - 590 Aligned: 245 Modelled: 262 Confidence: 98.4% Identity: 23% PDB header:hydrolase Chain: D: PDB Molecule:putative glucanohydrolase pep1a; PDBTitle: glge isoform 1 from streptomyces coelicolor with maltose2 bound Phyre2 PDB 1ob0 chain A domain 2 Region: 176 - 582 Aligned: 252 Modelled: 258 Confidence: 98.3% Identity: 18% Fold: TIM beta/alpha-barrel Superfamily: (Trans)glycosidases Family: Amylase, catalytic domain Phyre2 PDB 6gya chain A Region: 176 - 583 Aligned: 258 Modelled: 265 Confidence: 98.3% Identity: 24% PDB header:hydrolase Chain: A: PDB Molecule:a-amylase; PDBTitle: amylase in complex with branched ligand Phyre2 PDB 4u33 chain F Region: 183 - 592 Aligned: 252 Modelled: 266 Confidence: 98.3% Identity: 23% PDB header:transferase Chain: F: PDB Molecule:alpha-1,4-glucan:maltose-1-phosphate maltosyltransferase; PDBTitle: structure of mtb glge bound to maltose Phyre2 PDB 1e43 chain A domain 2 Region: 176 - 583 Aligned: 254 Modelled: 260 Confidence: 98.1% Identity: 17% Fold: TIM beta/alpha-barrel Superfamily: (Trans)glycosidases Family: Amylase, catalytic domain Phyre2 PDB 5do8 chain A Region: 179 - 602 Aligned: 279 Modelled: 288 Confidence: 98.1% Identity: 24% PDB header:hydrolase Chain: A: PDB Molecule:lmo0184 protein; PDBTitle: 1.8 angstrom crystal structure of listeria monocytogenes lmo01842 alpha-1,6-glucosidase Phyre2 PDB 1m53 chain A Region: 179 - 599 Aligned: 278 Modelled: 295 Confidence: 98.1% Identity: 18% PDB header:isomerase Chain: A: PDB Molecule:isomaltulose synthase; PDBTitle: crystal structure of isomaltulose synthase (pali) from2 klebsiella sp. lx3 Phyre2 PDB 4gkl chain A Region: 193 - 527 Aligned: 174 Modelled: 189 Confidence: 98.1% Identity: 13% PDB header:hydrolase Chain: A: PDB Molecule:alpha-amylase; PDBTitle: crystal structure of a noncanonic maltogenic alpha-amylase amyb from2 thermotoga neapolitana Phyre2 PDB 1ht6 chain A domain 2 Region: 176 - 602 Aligned: 258 Modelled: 264 Confidence: 98.0% Identity: 25% Fold: TIM beta/alpha-barrel Superfamily: (Trans)glycosidases Family: Amylase, catalytic domain Phyre2 PDB 1uok chain A domain 2 Region: 179 - 675 Aligned: 309 Modelled: 328 Confidence: 98.0% Identity: 17% Fold: TIM beta/alpha-barrel Superfamily: (Trans)glycosidases Family: Amylase, catalytic domain Phyre2 PDB 3a47 chain A Region: 179 - 599 Aligned: 273 Modelled: 295 Confidence: 98.0% Identity: 16% PDB header:hydrolase Chain: A: PDB Molecule:oligo-1,6-glucosidase; PDBTitle: crystal structure of isomaltase from saccharomyces cerevisiae Phyre2 PDB 5zxg chain B Region: 186 - 602 Aligned: 250 Modelled: 267 Confidence: 98.0% Identity: 19% PDB header:hydrolase Chain: B: PDB Molecule:cyclic maltosyl-maltose hydrolase; PDBTitle: cyclic alpha-maltosyl-(1-->6)-maltose hydrolase from arthrobacter2 globiformis, ligand-free form Phyre2 PDB 1ua7 chain A domain 2 Region: 176 - 601 Aligned: 266 Modelled: 284 Confidence: 97.9% Identity: 14% Fold: TIM beta/alpha-barrel Superfamily: (Trans)glycosidases Family: Amylase, catalytic domain Phyre2 PDB 1lwh chain A domain 2 Region: 179 - 584 Aligned: 242 Modelled: 262 Confidence: 97.9% Identity: 15% Fold: TIM beta/alpha-barrel Superfamily: (Trans)glycosidases Family: Amylase, catalytic domain Phyre2 1 c5b68A_ 2 c4s3rA_ 3 d1x1na1 4 d1eswa_ 5 d1tz7a1 6 c4e2oA_ 7 c3zt5D_ 8 d1ob0a2 9 c6gyaA_ 10 c4u33F_ 11 d1e43a2 12 c5do8A_ 13 c1m53A_ 14 c4gklA_ 15 d1ht6a2 16 d1uoka2 17 c3a47A_ 18 c5zxgB_ 19 d1ua7a2 20 d1lwha2 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251