1

.

.

.

.

.

.

.

.

10

.

.

.

.

.

.

.

.

.

20

.

.

.

.

.

.

.

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30

.

.

.

.

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40

.

.

.

.

.

.

.

.

.

50

.

.

.

.

.

.

.

.

.

60

Sequence

M

D

F

G

A

L

P

P

E

I

N

S

G

R

M

Y

C

G

P

G

S

G

P

M

L

A

A

A

A

A

W

D

G

V

A

V

E

L

G

L

A

A

T

G

Y

A

S

V

I

A

E

L

T

G

A

P

W

V

G

A

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

.

.

.

.

.

.

.

70

.

.

.

.

.

.

.

.

.

80

.

.

.

.

.

.

.

.

.

90

.

.

.

.

.

.

.

.

.

100

.

.

.

.

.

.

.

.

.

110

.

.

.

.

.

.

.

.

.

120

Sequence

A

S

L

S

M

V

A

A

A

T

P

Y

V

A

W

L

S

Q

A

A

A

R

A

E

Q

A

G

M

Q

A

A

A

A

A

A

A

Y

E

A

A

F

V

M

T

V

P

P

P

V

I

T

A

N

R

V

L

V

M

T

L

Secondary structure

SS confidence

Disorder

Disorder confidence

.

.

.

.

.

.

.

.

.

130

.

.

.

.

.

.

.

.

.

140

.

.

.

.

.

.

.

.

.

150

.

.

.

.

.

.

.

.

.

160

.

.

.

.

.

.

.

.

.

170

.

.

.

.

.

.

.

.

.

180

Sequence

I

A

T

N

F

F

G

Q

N

S

A

A

I

A

V

A

E

A

Q

Y

A

E

M

W

A

Q

D

A

V

A

M

Y

G

Y

A

A

A

S

A

S

A

S

R

L

I

P

F

A

A

P

P

K

T

T

N

S

A

G

V

V

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

.

.

.

.

.

.

.

190

.

.

.

.

.

.

.

.

.

200

.

.

.

.

.

.

.

.

.

210

.

.

.

.

.

.

.

.

.

220

.

.

.

.

.

.

.

.

.

230

.

.

.

.

.

.

.

.

.

240

Sequence

A

Q

A

V

A

S

V

S

W

P

N

P

N

D

W

W

L

V

R

L

L

G

S

I

T

P

T

E

R

T

T

I

V

R

L

L

G

Q

S

Y

L

A

T

G

M

A

R

F

L

T

S

I

A

Q

Q

L

T

F

G

P

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

.

.

.

.

.

.

.

250

.

.

.

.

.

.

.

.

.

260

.

.

.

.

.

.

.

.

.

270

.

.

.

.

.

.

.

.

.

280

.

.

.

.

.

.

.

.

.

290

.

.

.

.

.

.

.

.

.

300

Sequence

G

G

T

T

A

G

S

G

G

A

W

Y

P

T

P

Q

F

A

G

L

G

A

G

P

A

V

S

A

S

L

A

R

A

E

P

V

G

R

L

S

V

P

P

S

W

A

V

A

A

P

A

F

A

E

K

P

E

A

G

T

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

.

.

.

.

.

.

.

310

.

.

.

.

.

.

.

.

.

320

.

.

.

.

.

.

.

.

.

330

.

.

.

.

.

.

.

.

.

340

.

.

.

.

.

.

.

.

.

350

Sequence

P

M

S

V

I

G

E

A

S

S

C

G

Q

G

G

L

L

R

G

I

P

L

A

R

A

G

R

R

T

G

A

F

A

H

R

Y

G

F

R

H

S

V

I

T

R

S

P

S

A

G

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 50%) Alpha helix ( 57%) Beta strand ( 1%) TM helix ( 5%) (View membrane topology prediction)

Rank Aligned region

PDB 5xfs chain B Region: 1 - 173 Aligned: 173 Modelled: 173 Confidence: 100.0% Identity: 62% PDB header:protein transport Chain: B: PDB Molecule:ppe family protein ppe15; PDBTitle: crystal structure of pe8-ppe15 in complex with espg5 from m.2 tuberculosis Phyre2 PDB 2g38 chain B domain 1 Region: 3 - 173 Aligned: 170 Modelled: 171 Confidence: 100.0% Identity: 35% Fold: Ferritin-like Superfamily: PE/PPE dimer-like Family: PPE Phyre2 PDB 2g38 chain B Region: 3 - 173 Aligned: 170 Modelled: 171 Confidence: 100.0% Identity: 35% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:ppe family protein; PDBTitle: a pe/ppe protein complex from mycobacterium tuberculosis Phyre2 PDB 4xy3 chain A Region: 4 - 173 Aligned: 154 Modelled: 170 Confidence: 100.0% Identity: 16% PDB header:protein transport Chain: A: PDB Molecule:esx-1 secretion-associated protein espb; PDBTitle: structure of esx-1 secreted protein espb Phyre2 PDB 4wj2 chain A Region: 14 - 177 Aligned: 143 Modelled: 151 Confidence: 98.6% Identity: 17% PDB header:unknown function Chain: A: PDB Molecule:antigen mtb48; PDBTitle: mycobacterial protein Phyre2 PDB 2vs0 chain B Region: 21 - 100 Aligned: 79 Modelled: 80 Confidence: 98.0% Identity: 14% PDB header:cell invasion Chain: B: PDB Molecule:virulence factor esxa; PDBTitle: structural analysis of homodimeric staphylococcal aureus2 virulence factor esxa Phyre2 PDB 4iog chain D Region: 21 - 99 Aligned: 78 Modelled: 79 Confidence: 97.8% Identity: 14% PDB header:unknown function Chain: D: PDB Molecule:secreted protein esxb; PDBTitle: the crystal structure of a secreted protein esxb (wild-type, in p212 space group) from bacillus anthracis str. sterne Phyre2 PDB 3gvm chain A Region: 21 - 100 Aligned: 79 Modelled: 80 Confidence: 97.7% Identity: 14% PDB header:viral protein Chain: A: PDB Molecule:putative uncharacterized protein sag1039; PDBTitle: structure of the homodimeric wxg-100 family protein from streptococcus2 agalactiae Phyre2 PDB 3zbh chain C Region: 21 - 100 Aligned: 79 Modelled: 80 Confidence: 97.6% Identity: 15% PDB header:unknown function Chain: C: PDB Molecule:esxa; PDBTitle: geobacillus thermodenitrificans esxa crystal form i Phyre2 PDB 1wa8 chain A domain 1 Region: 21 - 100 Aligned: 79 Modelled: 80 Confidence: 97.2% Identity: 18% Fold: Ferritin-like Superfamily: EsxAB dimer-like Family: ESAT-6 like Phyre2 PDB 4lws chain B Region: 21 - 98 Aligned: 77 Modelled: 78 Confidence: 96.3% Identity: 17% PDB header:unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: esxa : esxb (semet) hetero-dimer from thermomonospora curvata Phyre2 PDB 1wa8 chain B domain 1 Region: 22 - 100 Aligned: 78 Modelled: 79 Confidence: 95.8% Identity: 17% Fold: Ferritin-like Superfamily: EsxAB dimer-like Family: ESAT-6 like Phyre2 PDB 4lws chain A Region: 21 - 100 Aligned: 79 Modelled: 77 Confidence: 95.4% Identity: 19% PDB header:unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: esxa : esxb (semet) hetero-dimer from thermomonospora curvata Phyre2 PDB 4i0x chain A Region: 23 - 97 Aligned: 74 Modelled: 75 Confidence: 92.1% Identity: 14% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:esat-6-like protein mab_3112; PDBTitle: crystal structure of the mycobacterum abscessus esxef (mab_3112-2 mab_3113) complex Phyre2 PDB 2kg7 chain B Region: 31 - 100 Aligned: 69 Modelled: 70 Confidence: 91.6% Identity: 17% PDB header:unknown function Chain: B: PDB Molecule:esat-6-like protein esxh; PDBTitle: structure and features of the complex formed by the tuberculosis2 virulence factors rv0287 and rv0288 Phyre2 PDB 4i0x chain J Region: 32 - 97 Aligned: 61 Modelled: 66 Confidence: 65.5% Identity: 23% PDB header:structural genomics, unknown function Chain: J: PDB Molecule:esat-6-like protein mab_3113; PDBTitle: crystal structure of the mycobacterum abscessus esxef (mab_3112-2 mab_3113) complex Phyre2 PDB 5frg chain A Region: 2 - 9 Aligned: 8 Modelled: 8 Confidence: 19.4% Identity: 75% PDB header:protein binding Chain: A: PDB Molecule:formin-binding protein 1-like; PDBTitle: the nmr structure of the cdc42-interacting region of toca1 Phyre2 PDB 1bkv chain A Region: 8 - 23 Aligned: 16 Modelled: 16 Confidence: 14.9% Identity: 50% PDB header:structural protein Chain: A: PDB Molecule:t3-785; PDBTitle: collagen Phyre2 PDB 1bkv chain B Region: 8 - 23 Aligned: 16 Modelled: 16 Confidence: 14.1% Identity: 50% PDB header:structural protein Chain: B: PDB Molecule:t3-785; PDBTitle: collagen Phyre2 PDB 1bkv chain C Region: 8 - 23 Aligned: 16 Modelled: 16 Confidence: 14.1% Identity: 50% PDB header:structural protein Chain: C: PDB Molecule:t3-785; PDBTitle: collagen Phyre2 1 c5xfsB_ 2 d2g38b1 3 c2g38B_ 4 c4xy3A_ 5 c4wj2A_ 6 c2vs0B_ 7 c4iogD_ 8 c3gvmA_ 9 c3zbhC_ 10 d1wa8a1 11 c4lwsB_ 12 d1wa8b1 13 c4lwsA_ 14 c4i0xA_ 15 c2kg7B_ 16 c4i0xJ_ 17 c5frgA_ 18 c1bkvA_ 19 c1bkvB_ 20 c1bkvC_ 21 22 23 24 25