1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	T	D	R	V	S	V	G	N	L	R	I	A	R	V	L	Y	D	F	V	N	N	E	A	L	P	G	T	D	I	D	P	D	S	F	W	A	G	V	D	K	V	V	A	D	L	T	P	Q	N	Q	A	L	L	N	A	R	D	E	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		Q	A	Q	I	D	K	W	H	R	R	R	V	I	E	P	I	D	M	D	A	Y	R	Q	F	L	T	E	I	G	Y	L	L	P	E	P	D	D	F	T	I	T	T	S	G	V	D	A	E	I	T	T	T	A	G	P	Q	L	V	V	P
Secondary structure
SS confidence
Disorder											?	?	?	?	?	?	?	?																					?					?			?						?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		V	L	N	A	R	F	A	L	N	A	A	N	A	R	W	G	S	L	Y	D	A	L	Y	G	T	D	V	I	P	E	T	D	G	A	E	K	G	P	T	Y	N	K	V	R	G	D	K	V	I	A	Y	A	R	K	F	L	D	D	S	V
Secondary structure
SS confidence
Disorder				?	?																										?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		P	L	S	S	G	S	F	G	D	A	T	G	F	T	V	Q	D	G	Q	L	V	V	A	L	P	D	K	S	T	G	L	A	N	P	G	Q	F	A	G	Y	T	G	A	A	E	S	P	T	S	V	L	L	I	N	H	G	L	H	I	E
Secondary structure
SS confidence
Disorder			?																												?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		I	L	I	D	P	E	S	Q	V	G	T	T	D	R	A	G	V	K	D	V	I	L	E	S	A	I	T	T	I	M	D	F	E	D	S	V	A	A	V	D	A	A	D	K	V	L	G	Y	R	N	W	L	G	L	N	K	G	D	L	A
Secondary structure
SS confidence
Disorder									?	?		?	?	?	?												?													?	?		?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		A	A	V	D	K	D	G	T	A	F	L	R	V	L	N	R	D	R	N	Y	T	A	P	G	G	G	Q	F	T	L	P	G	R	S	L	M	F	V	R	N	V	G	H	L	M	T	N	D	A	I	V	D	T	D	G	S	E	V	F	E
Secondary structure
SS confidence
Disorder		?													?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		G	I	M	D	A	L	F	T	G	L	I	A	I	H	G	L	K	A	S	D	V	N	G	P	L	I	N	S	R	T	G	S	I	Y	I	V	K	P	K	M	H	G	P	A	E	V	A	F	T	C	E	L	F	S	R	V	E	D	V	L
Secondary structure
SS confidence
Disorder																					?	?	?	?		?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		G	L	P	Q	N	T	M	K	I	G	I	M	D	E	E	R	R	T	T	V	N	L	K	A	C	I	K	A	A	A	D	R	V	V	F	I	N	T	G	F	L	D	R	T	G	D	E	I	H	T	S	M	E	A	G	P	M	V	R	K
Secondary structure
SS confidence
Disorder																																																						?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		G	T	M	K	S	Q	P	W	I	L	A	Y	E	D	H	N	V	D	A	G	L	A	A	G	F	S	G	R	A	Q	V	G	K	G	M	W	T	M	T	E	L	M	A	D	M	V	E	T	K	I	A	Q	P	R	A	G	A	S	T	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?																				?	?			?	?	?	?	?	?	?	?	?																	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560	.	.	.	.	.	.	.	.	.	570	.	.	.	.	.	.	.	.	.	580	.	.	.	.	.	.	.	.	.	590	.	.	.	.	.	.	.	.	.	600
Sequence		W	V	P	S	P	T	A	A	T	L	H	A	L	H	Y	H	Q	V	D	V	A	A	V	Q	Q	G	L	A	G	K	R	R	A	T	I	E	Q	L	L	T	I	P	L	A	K	E	L	A	W	A	P	D	E	I	R	E	E	V	D	N
Secondary structure
SS confidence
Disorder																				?		?	?	?	?	?	?	?	?	?	?	?	?								?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	610	.	.	.	.	.	.	.	.	.	620	.	.	.	.	.	.	.	.	.	630	.	.	.	.	.	.	.	.	.	640	.	.	.	.	.	.	.	.	.	650	.	.	.	.	.	.	.	.	.	660
Sequence		N	C	Q	S	I	L	G	Y	V	V	R	W	V	D	Q	G	V	G	C	S	K	V	P	D	I	H	D	V	A	L	M	E	D	R	A	T	L	R	I	S	S	Q	L	L	A	N	W	L	R	H	G	V	I	T	S	A	D	V	R	A
Secondary structure
SS confidence
Disorder																																																								?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	670	.	.	.	.	.	.	.	.	.	680	.	.	.	.	.	.	.	.	.	690	.	.	.	.	.	.	.	.	.	700	.	.	.	.	.	.	.	.	.	710	.	.	.	.	.	.	.	.	.	720
Sequence		S	L	E	R	M	A	P	L	V	D	R	Q	N	A	G	D	V	A	Y	R	P	M	A	P	N	F	D	D	S	I	A	F	L	A	A	Q	E	L	I	L	S	G	A	Q	Q	P	N	G	Y	T	E	P	I	L	H	R	R	R	R	E
Secondary structure
SS confidence
Disorder															?		?	?		?			?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	730	.	.	.	.	.	.	.	.	.	740	.
Sequence		F	K	A	R	A	A	E	K	P	A	P	S	D	R	A	G	D	D	A	A	R
Secondary structure
SS confidence
Disorder						?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 21%) Alpha helix ( 48%) Beta strand ( 9%)

Rank Aligned region

PDB 5vfb chain B Region: 2 - 724 Aligned: 720 Modelled: 723 Confidence: 100.0% Identity: 69% PDB header:transferase Chain: B: PDB Molecule:malate synthase g; PDBTitle: 1.36 angstrom resolution crystal structure of malate synthase g from2 pseudomonas aeruginosa in complex with glycolic acid. Phyre2 PDB 1n8i chain A Region: 2 - 727 Aligned: 701 Modelled: 726 Confidence: 100.0% Identity: 100% Fold: TIM beta/alpha-barrel Superfamily: Malate synthase G Family: Malate synthase G Phyre2 PDB 1d8c chain A Region: 3 - 723 Aligned: 702 Modelled: 721 Confidence: 100.0% Identity: 57% Fold: TIM beta/alpha-barrel Superfamily: Malate synthase G Family: Malate synthase G Phyre2 PDB 3cuz chain A Region: 269 - 672 Aligned: 360 Modelled: 385 Confidence: 100.0% Identity: 22% PDB header:transferase Chain: A: PDB Molecule:malate synthase a; PDBTitle: atomic resolution structures of escherichia coli and2 bacillis anthracis malate synthase a: comparison with3 isoform g and implications for structure based drug design Phyre2 PDB 3cux chain A Region: 269 - 657 Aligned: 332 Modelled: 369 Confidence: 100.0% Identity: 20% PDB header:transferase Chain: A: PDB Molecule:malate synthase; PDBTitle: atomic resolution structures of escherichia coli and2 bacillis anthracis malate synthase a: comparison with3 isoform g and implications for structure based drug design Phyre2 PDB 4roq chain A Region: 269 - 651 Aligned: 271 Modelled: 301 Confidence: 99.9% Identity: 21% PDB header:lyase Chain: A: PDB Molecule:malyl-coa lyase/beta-methylmalyl-coa lyase; PDBTitle: crystal structure of malyl-coa lyase from methylobacterium extorquens Phyre2 PDB 5vxs chain F Region: 269 - 646 Aligned: 256 Modelled: 296 Confidence: 99.9% Identity: 21% PDB header:lyase Chain: F: PDB Molecule:citrate lyase subunit beta-like protein, mitochondrial; PDBTitle: crystal structure analysis of human clybl in apo form Phyre2 PDB 4l9z chain A Region: 269 - 647 Aligned: 268 Modelled: 315 Confidence: 99.9% Identity: 20% PDB header:lyase Chain: A: PDB Molecule:malyl-coa lyase; PDBTitle: crystal structure of rhodobacter sphaeroides malyl-coa lyase in2 complex with magnesium, oxalate, and coa Phyre2 PDB 4l7z chain C Region: 269 - 644 Aligned: 261 Modelled: 317 Confidence: 99.8% Identity: 21% PDB header:lyase Chain: C: PDB Molecule:hpch/hpai aldolase; PDBTitle: crystal structure of chloroflexus aurantiacus malyl-coa lyase Phyre2 PDB 3pug chain A Region: 262 - 671 Aligned: 300 Modelled: 317 Confidence: 99.8% Identity: 16% PDB header:transferase Chain: A: PDB Molecule:malate synthase; PDBTitle: haloferax volcanii malate synthase native at 3mm glyoxylate Phyre2 PDB 6arb chain A Region: 269 - 646 Aligned: 235 Modelled: 270 Confidence: 99.7% Identity: 24% PDB header:lyase Chain: A: PDB Molecule:citrate lyase subunit beta-like protein; PDBTitle: crystal structure of protein cite from mycobacterium tuberculosis in2 complex with magnesium, pyruvate and coenzyme a Phyre2 PDB 3qll chain B Region: 269 - 555 Aligned: 187 Modelled: 215 Confidence: 99.6% Identity: 22% PDB header:lyase Chain: B: PDB Molecule:citrate lyase; PDBTitle: crystal structure of ripc from yersinia pestis Phyre2 PDB 3r4i chain B Region: 258 - 657 Aligned: 283 Modelled: 305 Confidence: 99.6% Identity: 18% PDB header:lyase Chain: B: PDB Molecule:citrate lyase; PDBTitle: crystal structure of a citrate lyase (bxe_b2899) from burkholderia2 xenovorans lb400 at 2.24 a resolution Phyre2 PDB 1u5v chain A Region: 269 - 555 Aligned: 190 Modelled: 210 Confidence: 99.6% Identity: 25% PDB header:lyase Chain: A: PDB Molecule:cite; PDBTitle: structure of cite complexed with triphosphate group of atp form2 mycobacterium tuberculosis Phyre2 PDB 3qqw chain C Region: 257 - 555 Aligned: 227 Modelled: 238 Confidence: 99.6% Identity: 17% PDB header:lyase Chain: C: PDB Molecule:putative citrate lyase; PDBTitle: crystal structure of a putative lyase (reut_b4148) from ralstonia2 eutropha jmp134 at 2.44 a resolution Phyre2 PDB 1u5h chain A Region: 269 - 555 Aligned: 190 Modelled: 210 Confidence: 99.5% Identity: 26% Fold: TIM beta/alpha-barrel Superfamily: Phosphoenolpyruvate/pyruvate domain Family: HpcH/HpaI aldolase Phyre2 PDB 1sgj chain B Region: 391 - 555 Aligned: 141 Modelled: 165 Confidence: 99.5% Identity: 17% PDB header:lyase Chain: B: PDB Molecule:citrate lyase, beta subunit; PDBTitle: crystal structure of citrate lyase beta subunit Phyre2 PDB 1sgj chain A Region: 391 - 555 Aligned: 141 Modelled: 165 Confidence: 99.5% Identity: 17% Fold: TIM beta/alpha-barrel Superfamily: Phosphoenolpyruvate/pyruvate domain Family: HpcH/HpaI aldolase Phyre2 PDB 2hro chain A Region: 388 - 523 Aligned: 131 Modelled: 136 Confidence: 96.6% Identity: 18% PDB header:transferase Chain: A: PDB Molecule:phosphoenolpyruvate-protein phosphotransferase; PDBTitle: structure of the full-lenght enzyme i of the pts system from2 staphylococcus carnosus Phyre2 PDB 2hwg chain A Region: 388 - 523 Aligned: 131 Modelled: 136 Confidence: 96.6% Identity: 15% PDB header:transferase Chain: A: PDB Molecule:phosphoenolpyruvate-protein phosphotransferase; PDBTitle: structure of phosphorylated enzyme i of the phosphoenolpyruvate:sugar2 phosphotransferase system Phyre2 1 c5vfbB_ 2 d1n8ia_ 3 d1d8ca_ 4 c3cuzA_ 5 c3cuxA_ 6 c4roqA_ 7 c5vxsF_ 8 c4l9zA_ 9 c4l7zC_ 10 c3pugA_ 11 c6arbA_ 12 c3qllB_ 13 c3r4iB_ 14 c1u5vA_ 15 c3qqwC_ 16 d1u5ha_ 17 c1sgjB_ 18 d1sgja_ 19 c2hroA_ 20 c2hwgA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99