1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	P	D	T	M	V	T	T	D	V	I	K	S	A	V	Q	L	A	C	R	A	P	S	L	H	N	S	Q	P	W	R	W	I	A	E	D	H	T	V	A	L	F	L	D	K	D	R	V	L	Y	A	T	D	H	S	G	R	E	A	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		L	G	C	G	A	V	L	D	H	F	R	V	A	M	A	A	A	G	T	T	A	N	V	E	R	F	P	N	P	N	D	P	L	H	L	A	S	I	D	F	S	P	A	D	F	V	T	E	G	H	R	L	R	A	D	A	I	L	L	R
Secondary structure
SS confidence
Disorder																																										?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		R	T	D	R	L	P	F	A	E	P	P	D	W	D	L	V	E	S	Q	L	R	T	T	V	T	A	D	T	V	R	I	D	V	I	A	D	D	M	R	P	E	L	A	A	A	S	K	L	T	E	S	L	R	L	Y	D	S	S	Y	H
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		A	E	L	F	W	W	T	G	A	F	E	T	S	E	G	I	P	H	S	S	L	V	S	A	A	E	S	D	R	V	T	F	G	R	D	F	P	V	V	A	N	T	D	R	R	P	E	F	G	H	D	R	S	K	V	L	V	L	S	T
Secondary structure
SS confidence
Disorder													?									?	?	?	?	?	?	?	?	?	?		?	?		?		?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		Y	D	N	E	R	A	S	L	L	R	C	G	E	M	L	S	A	V	L	L	D	A	T	M	A	G	L	A	T	C	T	L	T	H	I	T	E	L	H	A	S	R	D	L	V	A	A	L	I	G	Q	P	A	T	P	Q	A	L	V	R
Secondary structure
SS confidence
Disorder																																																	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.
Sequence		V	G	L	A	P	E	M	E	E	P	P	P	A	T	P	R	R	P	I	D	E	V	F	H	V	R	A	K	D	H	R
Secondary structure
SS confidence
Disorder						?	?	?	?	?	?	?	?	?												?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 19%) Alpha helix ( 41%) Beta strand ( 14%)

Rank Aligned region

PDB 2ymv chain A Region: 3 - 326 Aligned: 323 Modelled: 324 Confidence: 100.0% Identity: 54% PDB header:oxidoreductase Chain: A: PDB Molecule:acg nitroreductase; PDBTitle: structure of reduced m smegmatis 5246, a homologue of m.2 tuberculosis acg Phyre2 PDB 2wzv chain B Region: 108 - 327 Aligned: 216 Modelled: 219 Confidence: 100.0% Identity: 13% PDB header:oxidoreductase Chain: B: PDB Molecule:nfnb protein; PDBTitle: crystal structure of the fmn-dependent nitroreductase nfnb2 from mycobacterium smegmatis Phyre2 PDB 3gr3 chain B Region: 109 - 325 Aligned: 213 Modelled: 215 Confidence: 100.0% Identity: 15% PDB header:flavoprotein Chain: B: PDB Molecule:nitroreductase; PDBTitle: crystal structure of a nitroreductase-like family protein (pnba,2 bh06130) from bartonella henselae str. houston-1 at 1.45 a resolution Phyre2 PDB 2isl chain B Region: 103 - 331 Aligned: 208 Modelled: 213 Confidence: 99.9% Identity: 14% PDB header:flavoprotein Chain: B: PDB Molecule:blub; PDBTitle: blub bound to reduced flavin (fmnh2) and molecular oxygen.2 (clear crystal form) Phyre2 PDB 5ko8 chain B Region: 96 - 325 Aligned: 200 Modelled: 207 Confidence: 99.9% Identity: 20% PDB header:oxidoreductase Chain: B: PDB Molecule:nitroreductase; PDBTitle: crystal structure of haliscomenobacter hydrossis iodotyrosine2 deiodinase (iyd) bound to fmn and mono-iodotyrosine (i-tyr) Phyre2 PDB 5hdj chain A Region: 112 - 329 Aligned: 184 Modelled: 191 Confidence: 99.9% Identity: 16% PDB header:oxidoreductase Chain: A: PDB Molecule:nfra1; PDBTitle: structure of b. megaterium nfra1 Phyre2 PDB 3n2s chain D Region: 113 - 329 Aligned: 183 Modelled: 187 Confidence: 99.9% Identity: 19% PDB header:oxidoreductase Chain: D: PDB Molecule:nadph-dependent nitro/flavin reductase; PDBTitle: structure of nfra1 nitroreductase from b. subtilis Phyre2 PDB 3gh8 chain A Region: 111 - 325 Aligned: 188 Modelled: 192 Confidence: 99.9% Identity: 12% PDB header:oxidoreductase Chain: A: PDB Molecule:iodotyrosine dehalogenase 1; PDBTitle: crystal structure of mus musculus iodotyrosine deiodinase (iyd) bound2 to fmn and di-iodotyrosine (dit) Phyre2 PDB 1zch chain A domain 1 Region: 112 - 330 Aligned: 185 Modelled: 192 Confidence: 99.9% Identity: 15% Fold: FMN-dependent nitroreductase-like Superfamily: FMN-dependent nitroreductase-like Family: NADH oxidase/flavin reductase Phyre2 PDB 3gfa chain B Region: 113 - 326 Aligned: 196 Modelled: 214 Confidence: 99.9% Identity: 12% PDB header:oxidoreductase Chain: B: PDB Molecule:putative nitroreductase; PDBTitle: crystal structure of a putative nitroreductase in complex with fmn2 (cd3205) from clostridium difficile 630 at 1.35 a resolution Phyre2 PDB 1vfr chain A Region: 112 - 325 Aligned: 208 Modelled: 214 Confidence: 99.9% Identity: 9% Fold: FMN-dependent nitroreductase-like Superfamily: FMN-dependent nitroreductase-like Family: NADH oxidase/flavin reductase Phyre2 PDB 3eo8 chain A Region: 113 - 325 Aligned: 209 Modelled: 213 Confidence: 99.9% Identity: 11% PDB header:flavoprotein Chain: A: PDB Molecule:blub-like flavoprotein; PDBTitle: crystal structure of blub-like flavoprotein (yp_001089088.1) from2 clostridium difficile 630 at 1.74 a resolution Phyre2 PDB 1bkj chain A Region: 112 - 331 Aligned: 164 Modelled: 171 Confidence: 99.9% Identity: 19% Fold: FMN-dependent nitroreductase-like Superfamily: FMN-dependent nitroreductase-like Family: NADH oxidase/flavin reductase Phyre2 PDB 5hei chain E Region: 112 - 330 Aligned: 184 Modelled: 204 Confidence: 99.9% Identity: 18% PDB header:oxidoreductase Chain: E: PDB Molecule:nfra2; PDBTitle: structure of b. megaterium nfra2 Phyre2 PDB 4eo3 chain A Region: 115 - 325 Aligned: 157 Modelled: 175 Confidence: 99.9% Identity: 13% PDB header:oxidoreductase Chain: A: PDB Molecule:bacterioferritin comigratory protein/nadh dehydrogenase; PDBTitle: peroxiredoxin nitroreductase fusion enzyme Phyre2 PDB 3eof chain B Region: 115 - 329 Aligned: 179 Modelled: 185 Confidence: 99.9% Identity: 21% PDB header:oxidoreductase Chain: B: PDB Molecule:putative oxidoreductase; PDBTitle: crystal structure of putative oxidoreductase (yp_213212.1) from2 bacteroides fragilis nctc 9343 at 1.99 a resolution Phyre2 PDB 1nox chain A Region: 113 - 326 Aligned: 194 Modelled: 214 Confidence: 99.9% Identity: 18% Fold: FMN-dependent nitroreductase-like Superfamily: FMN-dependent nitroreductase-like Family: NADH oxidase/flavin reductase Phyre2 PDB 2b67 chain A domain 1 Region: 112 - 326 Aligned: 198 Modelled: 215 Confidence: 99.9% Identity: 11% Fold: FMN-dependent nitroreductase-like Superfamily: FMN-dependent nitroreductase-like Family: NADH oxidase/flavin reductase Phyre2 PDB 1f5v chain A Region: 112 - 330 Aligned: 179 Modelled: 186 Confidence: 99.9% Identity: 13% Fold: FMN-dependent nitroreductase-like Superfamily: FMN-dependent nitroreductase-like Family: NADH oxidase/flavin reductase Phyre2 PDB 3ek3 chain A Region: 112 - 325 Aligned: 179 Modelled: 183 Confidence: 99.9% Identity: 11% PDB header:flavoprotein Chain: A: PDB Molecule:nitroreductase; PDBTitle: crystal structure of nitroreductase with bound fmn (yp_211706.1) from2 bacteroides fragilis nctc 9343 at 1.70 a resolution Phyre2 1 c2ymvA_ 2 c2wzvB_ 3 c3gr3B_ 4 c2islB_ 5 c5ko8B_ 6 c5hdjA_ 7 c3n2sD_ 8 c3gh8A_ 9 d1zcha1 10 c3gfaB_ 11 d1vfra_ 12 c3eo8A_ 13 d1bkja_ 14 c5heiE_ 15 c4eo3A_ 16 c3eofB_ 17 d1noxa_ 18 d2b67a1 19 d1f5va_ 20 c3ek3A_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93