1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	I	A	A	D	D	D	T	E	K	S	M	M	D	M	A	R	A	E	R	A	E	L	A	A	F	L	T	T	L	T	L	Q	Q	W	E	T	P	S	L	C	A	G	W	S	V	K	E	V	V	A	H	M	I	S	Y	E	D	L	G
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		V	F	G	L	L	K	R	F	A	K	G	R	I	V	R	A	N	E	V	G	V	D	E	F	A	G	L	S	P	Q	E	L	A	D	Y	V	G	R	H	L	Q	P	R	G	L	T	A	G	F	G	G	M	I	A	L	V	D	G	M	I
Secondary structure
SS confidence
Disorder				?		?																																					?	?					?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		H	H	Q	D	I	R	R	P	L	G	Q	P	R	T	I	P	A	Q	R	L	D	R	V	L	R	L	M	P	K	N	P	R	L	R	A	R	P	R	I	K	G	L	R	L	R	A	T	D	L	D	W	T	I	G	T	G	P	E	V	T
Secondary structure
SS confidence
Disorder																																?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.
Sequence		G	P	G	E	A	L	L	M	A	M	A	G	R	P	A	A	V	S	D	L	S	G	P	G	K	P	T	L	A	G	R	L	G
Secondary structure
SS confidence
Disorder																		?		?												?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 12%) Alpha helix ( 57%) Beta strand ( 2%)

Rank Aligned region

PDB 2nsf chain A Region: 1 - 205 Aligned: 203 Modelled: 205 Confidence: 100.0% Identity: 18% PDB header:isomerase Chain: A: PDB Molecule:hypothetical protein cgl3021; PDBTitle: crystal structure of the mycothiol-dependent maleylpyruvate isomerase Phyre2 PDB 2nsf chain A domain 1 Region: 1 - 132 Aligned: 132 Modelled: 132 Confidence: 99.9% Identity: 17% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: Maleylpyruvate isomerase-like Phyre2 PDB 5cog chain B Region: 7 - 98 Aligned: 92 Modelled: 92 Confidence: 98.7% Identity: 11% PDB header:unknown function Chain: B: PDB Molecule:irc4; PDBTitle: crystal structure of yeast irc4 Phyre2 PDB 5civ chain A Region: 7 - 97 Aligned: 91 Modelled: 91 Confidence: 98.5% Identity: 14% PDB header:unknown function Chain: A: PDB Molecule:sibling bacteriocin; PDBTitle: sibling lethal factor precursor - dfsb Phyre2 PDB 4n6c chain B Region: 7 - 97 Aligned: 91 Modelled: 91 Confidence: 98.2% Identity: 11% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of the b1rzq2 protein from streptococcus pneumoniae.2 northeast structural genomics consortium (nesg) target spr36. Phyre2 PDB 2rd9 chain C Region: 12 - 97 Aligned: 86 Modelled: 86 Confidence: 98.1% Identity: 19% PDB header:hydrolase Chain: C: PDB Molecule:bh0186 protein; PDBTitle: crystal structure of a putative yfit-like metal-dependent hydrolase2 (bh0186) from bacillus halodurans c-125 at 2.30 a resolution Phyre2 PDB 1rxq chain A Region: 10 - 97 Aligned: 88 Modelled: 88 Confidence: 98.0% Identity: 18% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: YfiT-like putative metal-dependent hydrolases Phyre2 PDB 6iz2 chain A Region: 8 - 134 Aligned: 125 Modelled: 127 Confidence: 97.7% Identity: 7% PDB header:unknown function Chain: A: PDB Molecule:dinb/yfit family protein; PDBTitle: crystal structure of dinb/yfit protein dr0053 from d. radiodurans r1 Phyre2 PDB 5cof chain A Region: 3 - 97 Aligned: 95 Modelled: 94 Confidence: 97.6% Identity: 14% PDB header:unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of uncharacterised protein q1r1x2 from escherichia2 coli uti89 Phyre2 PDB 6anr chain A Region: 4 - 78 Aligned: 75 Modelled: 75 Confidence: 97.2% Identity: 19% PDB header:hydrolase Chain: A: PDB Molecule:colibactin self-protection protein clbs; PDBTitle: crystal structure of a self resistance protein clbs from colibactin2 biosynthetic gene cluster Phyre2 PDB 5cqv chain B Region: 7 - 97 Aligned: 91 Modelled: 91 Confidence: 97.2% Identity: 9% PDB header:unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of uncharacterized protein q8dwv2 from streptococcus2 agalactiae Phyre2 PDB 3cex chain B Region: 12 - 122 Aligned: 111 Modelled: 109 Confidence: 97.1% Identity: 14% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of the conserved protein of locus ef_3021 from2 enterococcus faecalis Phyre2 PDB 2hkv chain A domain 1 Region: 10 - 122 Aligned: 113 Modelled: 113 Confidence: 96.9% Identity: 11% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: DinB-like Phyre2 PDB 2ou6 chain A domain 1 Region: 12 - 126 Aligned: 115 Modelled: 115 Confidence: 96.8% Identity: 13% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: DinB-like Phyre2 PDB 3e4x chain B Region: 7 - 122 Aligned: 116 Modelled: 116 Confidence: 96.7% Identity: 9% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:apc36150; PDBTitle: crystal structure of putative metal-dependent hydrolases2 apc36150 Phyre2 PDB 2yqy chain B Region: 8 - 64 Aligned: 53 Modelled: 57 Confidence: 96.6% Identity: 25% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:hypothetical protein ttha0303; PDBTitle: crystal structure of tt2238, a four-helix bundle protein Phyre2 PDB 2qe9 chain B Region: 9 - 137 Aligned: 129 Modelled: 127 Confidence: 96.3% Identity: 11% PDB header:hydrolase Chain: B: PDB Molecule:uncharacterized protein yiza; PDBTitle: crystal structure of a putative metal-dependent hydrolase (yiza,2 bsu10800) from bacillus subtilis at 1.90 a resolution Phyre2 PDB 3di5 chain A Region: 8 - 83 Aligned: 76 Modelled: 76 Confidence: 96.3% Identity: 13% PDB header:metal binding protein Chain: A: PDB Molecule:dinb-like protein; PDBTitle: crystal structure of a dinb-like protein (bce_4655) from bacillus2 cereus atcc 10987 at 2.01 a resolution Phyre2 PDB 3dka chain A Region: 11 - 122 Aligned: 110 Modelled: 112 Confidence: 96.0% Identity: 12% PDB header:unknown function Chain: A: PDB Molecule:dinb-like protein; PDBTitle: crystal structure of a dinb-like protein (yjoa, bsu12410) from2 bacillus subtilis at 2.30 a resolution Phyre2 PDB 2p1a chain A domain 1 Region: 10 - 67 Aligned: 58 Modelled: 58 Confidence: 96.0% Identity: 14% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: DinB-like Phyre2 1 c2nsfA_ 2 d2nsfa1 3 c5cogB_ 4 c5civA_ 5 c4n6cB_ 6 c2rd9C_ 7 d1rxqa_ 8 c6iz2A_ 9 c5cofA_ 10 c6anrA_ 11 c5cqvB_ 12 c3cexB_ 13 d2hkva1 14 d2ou6a1 15 c3e4xB_ 16 c2yqyB_ 17 c2qe9B_ 18 c3di5A_ 19 c3dkaA_ 20 d2p1aa1 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38