1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	A	P	P	N	R	D	E	L	L	A	A	V	E	R	S	P	Q	A	A	A	A	H	D	R	A	G	W	V	G	L	F	T	G	D	A	R	V	E	D	P	V	G	S	Q	P	Q	V	G	H	E	A	I	G	R	F	Y	D	T	F
Secondary structure
SS confidence
Disorder		?	?	?	?	?																																									?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		I	G	P	R	D	I	T	F	H	R	D	L	D	I	V	S	G	T	V	V	L	R	D	L	E	L	E	V	A	M	D	S	A	V	T	V	F	I	P	A	F	L	R	Y	D	L	R	P	V	T	G	E	W	Q	I	A	A	L	R	A
Secondary structure
SS confidence
Disorder																																	?	?			?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		Y	W	E	L	P	A	M	M	L	Q	F	L	R	T	G	S	G	A	T	R	P	A	L	Q	L	S	R	A	L	L	G	N	Q	G	L	G	G	T	A	G	F	L	T	G	F	R	R	A	G	R	R	H	K	K	L	V	E	T	F	L
Secondary structure
SS confidence
Disorder													?	?	?	?	?	?	?	?	?					?	?					?	?	?															?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		N	A	A	S	R	A	D	K	S	A	A	Y	H	A	L	S	R	T	A	T	M	T	L	G	E	D	E	L	L	D	I	V	E	L	F	E	Q	L	R	G	A	S	W	T	K	V	T	G	A	G	S	T	V	A	V	S	L	A	S	D
Secondary structure
SS confidence
Disorder					?	?	?	?	?					?	?	?	?	?			?	?	?	?	?	?	?	?	?	?									?			?	?	?	?	?	?	?	?	?	?	?									?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.
Sequence		H	R	R	G	I	M	F	A	D	V	P	W	R	G	N	R	I	N	R	I	R	Y	F	P	A
Secondary structure
SS confidence
Disorder													?											?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 25%) Alpha helix ( 36%) Beta strand ( 35%)

Rank Aligned region

PDB 3rga chain A Region: 4 - 127 Aligned: 124 Modelled: 124 Confidence: 99.9% Identity: 20% PDB header:isomerase Chain: A: PDB Molecule:epoxide hydrolase; PDBTitle: crystal structure of epoxide hydrolase for polyether lasalocid a2 biosynthesis Phyre2 PDB 2a15 chain A domain 1 Region: 5 - 127 Aligned: 120 Modelled: 123 Confidence: 99.9% Identity: 31% Fold: Cystatin-like Superfamily: NTF2-like Family: Ketosteroid isomerase-like Phyre2 PDB 3wmd chain A Region: 5 - 127 Aligned: 120 Modelled: 123 Confidence: 99.9% Identity: 20% PDB header:isomerase Chain: A: PDB Molecule:probable monensin biosynthesis isomerase; PDBTitle: crystal structure of epoxide hydrolase monbi Phyre2 PDB 1oh0 chain A Region: 4 - 127 Aligned: 121 Modelled: 124 Confidence: 99.8% Identity: 20% Fold: Cystatin-like Superfamily: NTF2-like Family: Ketosteroid isomerase-like Phyre2 PDB 1ohp chain A domain 1 Region: 4 - 127 Aligned: 120 Modelled: 124 Confidence: 99.8% Identity: 26% Fold: Cystatin-like Superfamily: NTF2-like Family: Ketosteroid isomerase-like Phyre2 PDB 3f8x chain D Region: 7 - 142 Aligned: 127 Modelled: 131 Confidence: 99.8% Identity: 11% PDB header:isomerase Chain: D: PDB Molecule:putative delta-5-3-ketosteroid isomerase; PDBTitle: crystal structure of a putative delta-5-3-ketosteroid isomerase2 (eca2236) from pectobacterium atrosepticum scri1043 at 1.55 a3 resolution Phyre2 PDB 5cxo chain A Region: 4 - 127 Aligned: 121 Modelled: 124 Confidence: 99.8% Identity: 22% PDB header:hydrolase Chain: A: PDB Molecule:epoxide hydrolase; PDBTitle: intriguing role of epoxide hydrolase/cyclase-like enzyme salbiii in2 pyran ring formation in polyether salinomycin Phyre2 PDB 3mso chain A Region: 4 - 129 Aligned: 118 Modelled: 126 Confidence: 99.8% Identity: 18% PDB header:isomerase Chain: A: PDB Molecule:steroid delta-isomerase; PDBTitle: crystal structure of a steroid delta-isomerase (np_250810.1) from2 pseudomonas aeruginosa at 2.57 a resolution Phyre2 PDB 5aig chain A Region: 11 - 133 Aligned: 119 Modelled: 123 Confidence: 99.8% Identity: 15% PDB header:hydrolase Chain: A: PDB Molecule:limonene-1,2-epoxide hydrolase; PDBTitle: discovery and characterization of thermophilic limonene-1,2-epoxide2 hydrolases from hot spring metagenomic libraries. tomsk-sample-3 valpromide complex Phyre2 PDB 4h3u chain B Region: 11 - 132 Aligned: 118 Modelled: 122 Confidence: 99.7% Identity: 14% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:hypothetical protein; PDBTitle: crystal structure of hypothetical protein with ketosteroid isomerase-2 like protein fold from catenulispora acidiphila dsm 44928 Phyre2 PDB 3h3h chain A Region: 3 - 123 Aligned: 110 Modelled: 121 Confidence: 99.7% Identity: 14% PDB header:unknown function Chain: A: PDB Molecule:uncharacterized snoal-like protein; PDBTitle: crystal structure of a snoal-like protein of unknown function2 (bth_ii0226) from burkholderia thailandensis e264 at 1.60 a3 resolution Phyre2 PDB 2bng chain A domain 1 Region: 7 - 142 Aligned: 130 Modelled: 136 Confidence: 99.7% Identity: 17% Fold: Cystatin-like Superfamily: NTF2-like Family: Limonene-1,2-epoxide hydrolase-like Phyre2 PDB 3flj chain A Region: 11 - 129 Aligned: 110 Modelled: 119 Confidence: 99.7% Identity: 13% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein conserved in bacteria with a PDBTitle: crystal structure of uncharacterized protein conserved in bacteria2 with a cystatin-like fold (yp_168589.1) from silicibacter pomeroyi3 dss-3 at 2.00 a resolution Phyre2 PDB 3f8h chain A Region: 12 - 132 Aligned: 116 Modelled: 121 Confidence: 99.7% Identity: 19% PDB header:unknown function Chain: A: PDB Molecule:putative polyketide cyclase; PDBTitle: crystal structure of a putative polyketide cyclase (tm1040_3560) from2 silicibacter sp. tm1040 at 2.00 a resolution Phyre2 PDB 1z1s chain A domain 1 Region: 10 - 132 Aligned: 119 Modelled: 123 Confidence: 99.6% Identity: 17% Fold: Cystatin-like Superfamily: NTF2-like Family: PhzA/PhzB-like Phyre2 PDB 3en8 chain A domain 1 Region: 6 - 125 Aligned: 110 Modelled: 118 Confidence: 99.6% Identity: 15% Fold: Cystatin-like Superfamily: NTF2-like Family: Rpa4348-like Phyre2 PDB 3ke7 chain A Region: 2 - 122 Aligned: 117 Modelled: 121 Confidence: 99.6% Identity: 17% PDB header:isomerase Chain: A: PDB Molecule:putative ketosteroid isomerase; PDBTitle: crystal structure of putative ketosteroid isomerase (yp_001303366.1)2 from parabacteroides distasonis atcc 8503 at 1.45 a resolution Phyre2 PDB 1s5a chain A Region: 7 - 134 Aligned: 124 Modelled: 128 Confidence: 99.6% Identity: 15% Fold: Cystatin-like Superfamily: NTF2-like Family: PhzA/PhzB-like Phyre2 PDB 1z1s chain A Region: 2 - 132 Aligned: 124 Modelled: 131 Confidence: 99.6% Identity: 17% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:hypothetical protein pa3332; PDBTitle: crystal structure of putative isomerase pa3332 from2 pseudomonas aeruginosa Phyre2 PDB 5aii chain P Region: 10 - 132 Aligned: 118 Modelled: 123 Confidence: 99.6% Identity: 19% PDB header:hydrolase Chain: P: PDB Molecule:limonene-1,2-epoxide hydrolase; PDBTitle: discovery and characterization of thermophilic limonene-1,2-epoxide2 hydrolases from hot spring metagenomic libraries.3 ch55-sample-peg complex Phyre2 1 c3rgaA_ 2 d2a15a1 3 c3wmdA_ 4 d1oh0a_ 5 d1ohpa1 6 c3f8xD_ 7 c5cxoA_ 8 c3msoA_ 9 c5aigA_ 10 c4h3uB_ 11 c3h3hA_ 12 d2bnga1 13 c3fljA_ 14 c3f8hA_ 15 d1z1sa1 16 d3en8a1 17 c3ke7A_ 18 d1s5aa_ 19 c1z1sA_ 20 c5aiiP_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149