1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	L	T	R	G	E	V	R	A	L	P	A	D	A	V	V	L	S	A	D	D	A	A	D	L	S	D	R	V	Y	Q	V	R	C	A	A	E	D	V	V	T	A	L	D	E	G	A	A	A	T	E	L	R	D	L	C	D	E	L	I
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?			?																																?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.
Sequence		R	A	A	R	A	A	D	G	W	R	R	A	G	A
Secondary structure
SS confidence
Disorder										?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 28%) Alpha helix ( 80%) Beta strand ( 0%)

Rank Aligned region

PDB 1st6 chain A domain 3 Region: 46 - 73 Aligned: 28 Modelled: 28 Confidence: 31.4% Identity: 21% Fold: Four-helical up-and-down bundle Superfamily: alpha-catenin/vinculin-like Family: alpha-catenin/vinculin Phyre2 PDB 5ytp chain A Region: 15 - 59 Aligned: 45 Modelled: 45 Confidence: 30.7% Identity: 44% PDB header:dna binding protein Chain: A: PDB Molecule:ttha0139; PDBTitle: crystal structure of ttha0139 l34a from thermus thermophilus hb8 Phyre2 PDB 2a6q chain B domain 1 Region: 12 - 30 Aligned: 19 Modelled: 19 Confidence: 19.7% Identity: 21% Fold: YefM-like Superfamily: YefM-like Family: YefM-like Phyre2 PDB 1n26 chain A domain 1 Region: 2 - 18 Aligned: 17 Modelled: 17 Confidence: 16.7% Identity: 41% Fold: Immunoglobulin-like beta-sandwich Superfamily: Immunoglobulin Family: I set domains Phyre2 PDB 1st6 chain A domain 5 Region: 49 - 73 Aligned: 25 Modelled: 25 Confidence: 16.0% Identity: 20% Fold: Four-helical up-and-down bundle Superfamily: alpha-catenin/vinculin-like Family: alpha-catenin/vinculin Phyre2 PDB 3g5r chain A Region: 2 - 18 Aligned: 17 Modelled: 17 Confidence: 15.0% Identity: 41% PDB header:transferase Chain: A: PDB Molecule:methylenetetrahydrofolate--trna-(uracil-5-)- PDBTitle: crystal structure of thermus thermophilus trmfo in complex with2 tetrahydrofolate Phyre2 PDB 1st6 chain A domain 4 Region: 36 - 73 Aligned: 38 Modelled: 38 Confidence: 13.6% Identity: 18% Fold: Four-helical up-and-down bundle Superfamily: alpha-catenin/vinculin-like Family: alpha-catenin/vinculin Phyre2 PDB 3g5o chain A Region: 12 - 57 Aligned: 46 Modelled: 46 Confidence: 13.4% Identity: 24% PDB header:toxin/antitoxin Chain: A: PDB Molecule:uncharacterized protein rv2865; PDBTitle: the crystal structure of the toxin-antitoxin complex relbe2 (rv2865-2 2866) from mycobacterium tuberculosis Phyre2 PDB 3twe chain A Region: 20 - 33 Aligned: 14 Modelled: 14 Confidence: 12.6% Identity: 29% PDB header:unknown function Chain: A: PDB Molecule:alpha4h; PDBTitle: crystal structure of the de novo designed peptide alpha4h Phyre2 PDB 2le7 chain A Region: 59 - 74 Aligned: 16 Modelled: 16 Confidence: 12.3% Identity: 44% PDB header:transport protein Chain: A: PDB Molecule:potassium voltage-gated channel subfamily h member 2; PDBTitle: solution nmr structure of the s4s5 linker of herg potassium channel Phyre2 PDB 3twe chain B Region: 20 - 33 Aligned: 14 Modelled: 14 Confidence: 12.3% Identity: 29% PDB header:unknown function Chain: B: PDB Molecule:alpha4h; PDBTitle: crystal structure of the de novo designed peptide alpha4h Phyre2 PDB 6q5s chain B Region: 51 - 69 Aligned: 19 Modelled: 19 Confidence: 11.9% Identity: 32% PDB header:de novo protein Chain: B: PDB Molecule:apcc-tet; PDBTitle: crystal structure of a de novo designed antiparallel four-helix coiled2 coil apcc-tet Phyre2 PDB 6q5s chain C Region: 51 - 69 Aligned: 19 Modelled: 19 Confidence: 11.9% Identity: 32% PDB header:de novo protein Chain: C: PDB Molecule:apcc-tet; PDBTitle: crystal structure of a de novo designed antiparallel four-helix coiled2 coil apcc-tet Phyre2 PDB 6q5s chain D Region: 51 - 69 Aligned: 19 Modelled: 19 Confidence: 11.9% Identity: 32% PDB header:de novo protein Chain: D: PDB Molecule:apcc-tet; PDBTitle: crystal structure of a de novo designed antiparallel four-helix coiled2 coil apcc-tet Phyre2 PDB 6q5s chain A Region: 51 - 69 Aligned: 19 Modelled: 19 Confidence: 11.9% Identity: 32% PDB header:de novo protein Chain: A: PDB Molecule:apcc-tet; PDBTitle: crystal structure of a de novo designed antiparallel four-helix coiled2 coil apcc-tet Phyre2 PDB 3d55 chain A Region: 12 - 45 Aligned: 34 Modelled: 34 Confidence: 9.6% Identity: 35% PDB header:toxin inhibitor Chain: A: PDB Molecule:uncharacterized protein rv3357/mt3465; PDBTitle: crystal structure of m. tuberculosis yefm antitoxin Phyre2 PDB 3oei chain B Region: 13 - 33 Aligned: 21 Modelled: 21 Confidence: 9.1% Identity: 48% PDB header:toxin, protein binding Chain: B: PDB Molecule:relj (antitoxin rv3357); PDBTitle: crystal structure of mycobacterium tuberculosis reljk (rv3357-rv3358-2 relbe3) Phyre2 PDB 2vs0 chain B Region: 48 - 71 Aligned: 24 Modelled: 24 Confidence: 8.9% Identity: 8% PDB header:cell invasion Chain: B: PDB Molecule:virulence factor esxa; PDBTitle: structural analysis of homodimeric staphylococcal aureus2 virulence factor esxa Phyre2 PDB 2a6q chain A domain 1 Region: 12 - 33 Aligned: 22 Modelled: 22 Confidence: 8.1% Identity: 23% Fold: YefM-like Superfamily: YefM-like Family: YefM-like Phyre2 PDB 2lq0 chain A Region: 30 - 37 Aligned: 8 Modelled: 8 Confidence: 8.0% Identity: 63% PDB header:antifreeze protein Chain: A: PDB Molecule:de novo designed antifreeze peptide 1m; PDBTitle: solution structure of de novo designed antifreeze peptide 1m Phyre2 1 d1st6a3 2 c5ytpA_ 3 d2a6qb1 4 d1n26a1 5 d1st6a5 6 c3g5rA_ 7 d1st6a4 8 c3g5oA_ 9 c3tweA_ 10 c2le7A_ 11 c3tweB_ 12 c6q5sB_ 13 c6q5sC_ 14 c6q5sD_ 15 c6q5sA_ 16 c3d55A_ 17 c3oeiB_ 18 c2vs0B_ 19 d2a6qa1 20 c2lq0A_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39