1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	A	G	A	L	F	E	P	S	F	A	A	A	H	P	A	G	L	L	R	R	P	V	T	R	T	V	V	L	S	V	A	A	T	S	I	A	H	M	F	E	I	S	L	P	D	P	T	E	L	C	R	S	D	D	G	A	L	V	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?							?	?	?	?	?	?	?	?	?	?	?	?				?	?	?	?																		?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		A	I	E	D	C	A	R	V	E	A	A	A	S	A	R	R	L	S	A	I	A	E	L	T	G	R	R	T	G	A	D	Q	R	A	D	W	A	C	D	F	W	D	C	A	A	A	E	V	A	A	A	L	T	I	S	H	G	K	A	S
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		G	Q	M	H	L	S	L	A	L	N	R	L	P	Q	V	A	A	L	F	L	A	G	H	L	G	A	R	L	F	S	I	I	A	W	R	T	Y	L	V	R	D	P	H	A	L	S	L	L	D	A	A	L	A	E	H	A	G	A	W	G
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		P	L	S	A	P	K	L	E	K	A	I	D	S	W	I	D	R	Y	D	P	G	A	L	R	R	S	R	I	S	A	R	T	R	D	L	C	I	G	D	P	D	E	D	A	G	T	A	A	L	W	G	R	L	Y	A	T	D	A	A	M
Secondary structure
SS confidence
Disorder																									?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		L	D	R	R	L	T	E	M	A	H	G	V	C	E	D	D	P	R	T	L	A	Q	R	R	A	D	A	L	G	A	L	A	A	G	A	D	H	L	A	C	G	C	G	K	P	D	C	P	S	G	A	G	N	D	E	R	A	A	G	V
Secondary structure
SS confidence
Disorder											?	?	?		?	?	?	?	?	?	?													?	?	?		?												?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		V	I	H	V	V	A	D	A	S	A	L	D	A	Q	P	D	P	H	L	S	G	D	E	P	P	S	R	P	L	T	P	E	T	T	L	F	E	A	L	T	P	D	P	E	P	D	P	P	A	T	H	A	P	A	E	L	I	T	T	G
Secondary structure
SS confidence
Disorder								?				?	?		?		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?			?	?		?					?			?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		G	G	V	V	P	A	P	L	L	A	E	L	I	R	G	G	A	T	I	S	Q	V	R	H	P	G	D	L	A	A	E	P	H	Y	R	P	S	A	K	L	A	E	F	V	R	M	R	D	L	T	C	R	F	P	G	C	D	V	P	A
Secondary structure
SS confidence
Disorder																										?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		E	F	C	D	I	D	H	S	A	P	W	P	L	G	P	T	H	P	S	N	L	K	C	A	C	R	K	H	H	L	L	K	T	F	W	T	G	W	R	D	V	Q	L	P	D	G	T	V	I	W	T	A	P	N	G	H	T	Y	T	T
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		H	P	G	S	R	I	F	F	P	T	W	H	T	T	T	A	E	L	P	Q	T	S	T	A	A	V	N	V	D	A	R	G	L	M	M	P	R	R	R	R	T	R	A	A	E	L	A	H	R	I	N	A	E	R	A	L	N	D	A	Y
Secondary structure
SS confidence
Disorder														?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?										?
Disorder confidence
		.	.	.	.	.	.	.	.	.	550
Sequence		M	A	E	R	N	K	P	P	S	F
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 25%) Alpha helix ( 42%) Beta strand ( 7%)

Rank Aligned region

PDB 2qgp chain A Region: 391 - 452 Aligned: 62 Modelled: 62 Confidence: 98.8% Identity: 21% PDB header:hydrolase Chain: A: PDB Molecule:hnh endonuclease; PDBTitle: x-ray structure of the nhn endonuclease from geobacter2 metallireducens. northeast structural genomics consortium target3 gmr87. Phyre2 PDB 5mkw chain A Region: 397 - 453 Aligned: 57 Modelled: 57 Confidence: 98.4% Identity: 23% PDB header:hydrolase Chain: A: PDB Molecule:dna annealing helicase and endonuclease zranb3; PDBTitle: crystal structure of the human zranb3 hnh domain Phyre2 PDB 6ghc chain A Region: 362 - 455 Aligned: 94 Modelled: 94 Confidence: 98.0% Identity: 13% PDB header:hydrolase Chain: A: PDB Molecule:5-methylcytosine-specific restriction enzyme a; PDBTitle: modification dependent ecokmcra restriction endonuclease Phyre2 PDB 5x1h chain S Region: 398 - 470 Aligned: 70 Modelled: 73 Confidence: 97.9% Identity: 24% PDB header:protein transport Chain: S: PDB Molecule:icmj (dotn); PDBTitle: structure of legionella pneumophila dotn Phyre2 PDB 5h0m chain A Region: 398 - 453 Aligned: 56 Modelled: 56 Confidence: 97.7% Identity: 21% PDB header:hydrolase Chain: A: PDB Molecule:hnh endonuclease; PDBTitle: crystal structure of deep-sea thermophilic bacteriophage gve2 hnh2 endonuclease with zinc ion Phyre2 PDB 5vgb chain A Region: 408 - 452 Aligned: 43 Modelled: 45 Confidence: 97.4% Identity: 16% PDB header:hydrolase/hydrolase inhibitor Chain: A: PDB Molecule:crispr-associated endonuclease cas9; PDBTitle: crystal structure of nmecas9 hnh domain bound to anti-crispr acriic1 Phyre2 PDB 5zmm chain D Region: 394 - 455 Aligned: 62 Modelled: 62 Confidence: 97.1% Identity: 31% PDB header:dna binding protein Chain: D: PDB Molecule:uncharacterized protein mcra; PDBTitle: structure of the type iv phosphorothioation-dependent restriction2 endonuclease scomcra Phyre2 PDB 4oge chain A Region: 398 - 452 Aligned: 55 Modelled: 55 Confidence: 96.5% Identity: 20% PDB header:hydrolase Chain: A: PDB Molecule:hnh endonuclease domain protein; PDBTitle: crystal structure of the type ii-c cas9 enzyme from actinomyces2 naeslundii Phyre2 PDB 5axw chain A Region: 400 - 470 Aligned: 71 Modelled: 71 Confidence: 95.8% Identity: 11% PDB header:hydrolase/rna/dna Chain: A: PDB Molecule:crispr-associated endonuclease cas9; PDBTitle: crystal structure of staphylococcus aureus cas9 in complex with sgrna2 and target dna (ttgggt pam) Phyre2 PDB 6ghs chain A Region: 404 - 469 Aligned: 63 Modelled: 66 Confidence: 89.9% Identity: 24% PDB header:hydrolase Chain: A: PDB Molecule:tagi restriction endonuclease; PDBTitle: modification dependent tagi restriction endonuclease Phyre2 PDB 1vz0 chain A domain 1 Region: 106 - 177 Aligned: 65 Modelled: 72 Confidence: 88.3% Identity: 18% Fold: DNA/RNA-binding 3-helical bundle Superfamily: KorB DNA-binding domain-like Family: KorB DNA-binding domain-like Phyre2 PDB 3mky chain P Region: 106 - 191 Aligned: 80 Modelled: 86 Confidence: 81.7% Identity: 25% PDB header:dna binding protein/dna Chain: P: PDB Molecule:protein sopb; PDBTitle: structure of sopb(155-323)-18mer dna complex, i23 form Phyre2 PDB 3mkz chain U Region: 106 - 191 Aligned: 80 Modelled: 86 Confidence: 76.6% Identity: 25% PDB header:dna-binding protein/dna Chain: U: PDB Molecule:protein sopb; PDBTitle: structure of sopb(155-272)-18mer complex, p21 form Phyre2 PDB 2ho2 chain A domain 1 Region: 456 - 483 Aligned: 28 Modelled: 28 Confidence: 75.7% Identity: 43% Fold: WW domain-like Superfamily: WW domain Family: WW domain Phyre2 PDB 2ysc chain A domain 1 Region: 456 - 477 Aligned: 22 Modelled: 22 Confidence: 69.0% Identity: 41% Fold: WW domain-like Superfamily: WW domain Family: WW domain Phyre2 PDB 2jb0 chain B domain 1 Region: 424 - 449 Aligned: 26 Modelled: 26 Confidence: 66.1% Identity: 23% Fold: His-Me finger endonucleases Superfamily: His-Me finger endonucleases Family: HNH-motif Phyre2 PDB 4cmq chain B Region: 424 - 458 Aligned: 32 Modelled: 35 Confidence: 60.5% Identity: 22% PDB header:hydrolase Chain: B: PDB Molecule:crispr-associated endonuclease cas9/csn1; PDBTitle: crystal structure of mn-bound s.pyogenes cas9 Phyre2 PDB 2e45 chain A domain 1 Region: 456 - 484 Aligned: 29 Modelled: 29 Confidence: 56.0% Identity: 41% Fold: WW domain-like Superfamily: WW domain Family: WW domain Phyre2 PDB 4ld3 chain A Region: 453 - 484 Aligned: 32 Modelled: 32 Confidence: 49.9% Identity: 25% PDB header:structural protein, protein binding Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: structural analysis of the microcephaly protein cpap g-box domain2 suggests a role in centriole elongation. Phyre2 PDB 2gyk chain B domain 1 Region: 424 - 449 Aligned: 26 Modelled: 26 Confidence: 45.8% Identity: 23% Fold: His-Me finger endonucleases Superfamily: His-Me finger endonucleases Family: HNH-motif Phyre2 1 c2qgpA_ 2 c5mkwA_ 3 c6ghcA_ 4 c5x1hS_ 5 c5h0mA_ 6 c5vgbA_ 7 c5zmmD_ 8 c4ogeA_ 9 c5axwA_ 10 c6ghsA_ 11 d1vz0a1 12 c3mkyP_ 13 c3mkzU_ 14 d2ho2a1 15 d2ysca1 16 d2jb0b1 17 c4cmqB_ 18 d2e45a1 19 c4ld3A_ 20 d2gykb1 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99