1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	S	R	A	A	P	R	R	A	S	Q	S	Q	S	T	R	P	A	R	G	L	R	R	P	P	G	A	Q	E	V	G	Q	R	K	R	P	G	K	T	Q	K	A	R	Q	A	Q	E	A	T	K	S	R	P	A	T	R	S	D	V	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		P	A	G	R	S	T	R	A	R	R	T	R	Q	V	V	D	V	G	T	R	G	A	S	F	V	F	R	H	R	T	G	N	A	V	I	L	V	L	M	L	V	A	A	T	Q	L	F	F	L	Q	V	S	H	A	A	G	L	R	A	Q
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		A	A	G	Q	L	K	V	T	D	V	Q	P	A	A	R	G	S	I	V	D	R	N	N	D	R	L	A	F	T	I	E	A	R	A	L	T	F	Q	P	K	R	I	R	R	Q	L	E	E	A	R	K	K	T	S	A	A	P	D	P	Q
Secondary structure
SS confidence
Disorder				?	?	?	?					?	?	?	?																														?	?										?			?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		Q	R	L	R	D	I	A	Q	E	V	A	G	K	L	N	N	K	P	D	A	A	A	V	L	K	K	L	Q	S	D	E	T	F	V	Y	L	A	R	A	V	D	P	A	V	A	S	A	I	C	A	K	Y	P	E	V	G	A	E	R	Q
Secondary structure
SS confidence
Disorder															?	?	?	?	?	?								?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		D	L	R	Q	Y	P	G	G	S	L	A	A	N	V	V	G	G	I	D	W	D	G	H	G	L	L	G	L	E	D	S	L	D	A	V	L	A	G	T	D	G	S	V	T	Y	D	R	G	S	D	G	V	V	I	P	G	S	Y	R	N
Secondary structure
SS confidence
Disorder																								?																																?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		R	H	K	A	V	H	G	S	T	V	V	L	T	L	D	N	D	I	Q	F	Y	V	Q	Q	Q	V	Q	Q	A	K	N	L	S	G	A	H	N	V	S	A	V	V	L	D	A	K	T	G	E	V	L	A	M	A	N	D	N	T	F	D
Secondary structure
SS confidence
Disorder		?	?	?	?																														?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		P	S	Q	D	I	G	R	Q	G	D	K	Q	L	G	N	P	A	V	S	S	P	F	E	P	G	S	V	N	K	I	V	A	A	S	A	V	I	E	H	G	L	S	S	P	D	E	V	L	Q	V	P	G	S	I	Q	M	G	G	V	T
Secondary structure
SS confidence
Disorder								?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		V	H	D	A	W	E	H	G	V	M	P	Y	T	T	T	G	V	F	G	K	S	S	N	V	G	T	L	M	L	S	Q	R	V	G	P	E	R	Y	Y	D	M	L	R	K	F	G	L	G	Q	R	T	G	V	G	L	P	G	E	S	A
Secondary structure
SS confidence
Disorder																																																			?	?			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		G	L	V	P	P	I	D	Q	W	S	G	S	T	F	A	N	L	P	I	G	Q	G	L	S	M	T	L	L	Q	M	T	G	M	Y	Q	A	I	A	N	D	G	V	R	V	P	P	R	I	I	K	A	T	V	A	P	D	G	S	R	T
Secondary structure
SS confidence
Disorder										?			?	?																																												?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560	.	.	.	.	.	.	.	.	.	570	.	.	.	.	.	.	.	.	.	580	.	.	.	.	.	.	.	.	.	590	.	.	.	.	.	.	.	.	.	600
Sequence		E	E	P	R	P	D	D	I	R	V	V	S	A	Q	T	A	Q	T	V	R	Q	M	L	R	A	V	V	Q	R	D	P	M	G	Y	Q	Q	G	T	G	P	T	A	G	V	P	G	Y	Q	M	A	G	K	T	G	T	A	Q	Q	I	N
Secondary structure
SS confidence
Disorder			?				?																								?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	610	.	.	.	.	.	.	.	.	.	620	.	.	.	.	.	.	.	.	.	630	.	.	.	.	.	.	.	.	.	640	.	.	.	.	.	.	.	.	.	650	.	.	.	.	.	.	.	.	.	660
Sequence		P	G	C	G	C	Y	F	D	D	V	Y	W	I	T	F	A	G	I	A	T	A	D	N	P	R	Y	V	I	G	I	M	L	D	N	P	A	R	N	S	D	G	A	P	G	H	S	A	A	P	L	F	H	N	I	A	G	W	L	M	Q
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?																													?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	670	.	.	.	.	.	.	.	.	.
Sequence		R	E	N	V	P	L	S	P	D	P	G	P	P	L	V	L	Q	A	T
Secondary structure
SS confidence
Disorder												?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 24%) Alpha helix ( 32%) Beta strand ( 23%) TM helix ( 3%) (View membrane topology prediction)

Rank Aligned region

PDB 5u47 chain A Region: 129 - 673 Aligned: 529 Modelled: 545 Confidence: 100.0% Identity: 25% PDB header:lipid-binding protein Chain: A: PDB Molecule:penicillin binding protein 2x; PDBTitle: 1.95 angstrom resolution crystal structure of penicillin binding2 protein 2x from streptococcus thermophilus Phyre2 PDB 3oc2 chain A Region: 123 - 668 Aligned: 492 Modelled: 528 Confidence: 100.0% Identity: 35% PDB header:penicillin-binding protein Chain: A: PDB Molecule:penicillin-binding protein 3; PDBTitle: crystal structure of penicillin-binding protein 3 from pseudomonas2 aeruginosa Phyre2 PDB 4ye5 chain A Region: 125 - 677 Aligned: 533 Modelled: 553 Confidence: 100.0% Identity: 32% PDB header:penicillin binding protein Chain: A: PDB Molecule:peptidoglycan synthetase penicillin-binding protein 3; PDBTitle: the crystal structure of a peptidoglycan synthetase from2 bifidobacterium adolescentis atcc 15703 Phyre2 PDB 3pbq chain A Region: 126 - 671 Aligned: 491 Modelled: 528 Confidence: 100.0% Identity: 34% PDB header:hydrolase/antibiotic Chain: A: PDB Molecule:penicillin-binding protein 3; PDBTitle: crystal structure of pbp3 complexed with imipenem Phyre2 PDB 6g9f chain A Region: 124 - 659 Aligned: 490 Modelled: 521 Confidence: 100.0% Identity: 24% PDB header:hydrolase/antibiotic Chain: A: PDB Molecule:peptidoglycan d,d-transpeptidase mrda; PDBTitle: structural basis for the inhibition of e. coli pbp2 Phyre2 PDB 1pmd chain A Region: 135 - 674 Aligned: 516 Modelled: 516 Confidence: 100.0% Identity: 27% PDB header:peptidoglycan synthesis Chain: A: PDB Molecule:peptidoglycan synthesis multifunctional enzyme; PDBTitle: penicillin-binding protein 2x (pbp-2x) Phyre2 PDB 3ue3 chain A Region: 128 - 668 Aligned: 432 Modelled: 462 Confidence: 100.0% Identity: 34% PDB header:transferase Chain: A: PDB Molecule:septum formation, penicillin binding protein 3, PDBTitle: crystal structure of acinetobacter baumanni pbp3 Phyre2 PDB 5lp4 chain A Region: 127 - 662 Aligned: 484 Modelled: 536 Confidence: 100.0% Identity: 21% PDB header:hydrolase/antibiotic Chain: A: PDB Molecule:penicillin-binding protein 2 (pbp2); PDBTitle: penicillin-binding protein (pbp2) from helicobacter pylori Phyre2 PDB 3vsl chain B Region: 113 - 660 Aligned: 525 Modelled: 548 Confidence: 100.0% Identity: 22% PDB header:penicillin-binding protein Chain: B: PDB Molecule:penicillin-binding protein 3; PDBTitle: crystal structure of penicillin-binding protein 3 (pbp3) from2 methicilin-resistant staphylococcus aureus in the cefotaxime bound3 form. Phyre2 PDB 5tro chain A Region: 131 - 667 Aligned: 491 Modelled: 511 Confidence: 100.0% Identity: 27% PDB header:hydrolase Chain: A: PDB Molecule:penicillin-binding protein 1; PDBTitle: 1.8 angstrom resolution crystal structure of dimerization and2 transpeptidase domains (residues 39-608) of penicillin-binding3 protein 1 from staphylococcus aureus. Phyre2 PDB 3pbn chain A Region: 133 - 670 Aligned: 437 Modelled: 466 Confidence: 100.0% Identity: 36% PDB header:hydrolase/antibiotic Chain: A: PDB Molecule:penicillin-binding protein 3; PDBTitle: crystal structure of apo pbp3 from pseudomonas aeruginosa Phyre2 PDB 1mwu chain A Region: 107 - 669 Aligned: 501 Modelled: 547 Confidence: 100.0% Identity: 20% PDB header:biosynthetic protein Chain: A: PDB Molecule:penicillin-binding protein 2a; PDBTitle: structure of methicillin acyl-penicillin binding protein 2a from2 methicillin resistant staphylococcus aureus strain 27r at 2.60 a3 resolution. Phyre2 PDB 1qmf chain A Region: 130 - 672 Aligned: 428 Modelled: 436 Confidence: 100.0% Identity: 31% PDB header:cell cycle Chain: A: PDB Molecule:penicillin-binding protein 2x; PDBTitle: penicillin-binding protein 2x (pbp-2x) acyl-enzyme complex Phyre2 PDB 3equ chain B Region: 126 - 671 Aligned: 424 Modelled: 458 Confidence: 100.0% Identity: 36% PDB header:biosynthetic protein Chain: B: PDB Molecule:penicillin-binding protein 2; PDBTitle: crystal structure of penicillin-binding protein 2 from neisseria2 gonorrhoeae Phyre2 PDB 4ovd chain A Region: 128 - 666 Aligned: 417 Modelled: 433 Confidence: 100.0% Identity: 32% PDB header:transferase Chain: A: PDB Molecule:peptidoglycan glycosyltransferase; PDBTitle: crystal structure of a putative peptidoglycan glycosyltransferase from2 atopobium parvulum dsm 20469 Phyre2 PDB 4bjp chain A Region: 135 - 668 Aligned: 409 Modelled: 439 Confidence: 100.0% Identity: 33% PDB header:transferase Chain: A: PDB Molecule:penicillin binding protein transpeptidase domain protein; PDBTitle: crystal structure of e. coli penicillin binding protein 3 Phyre2 PDB 5dvy chain A Region: 107 - 663 Aligned: 505 Modelled: 523 Confidence: 100.0% Identity: 24% PDB header:penicillin-binding protein Chain: A: PDB Molecule:penicillin binding protein 2 prime; PDBTitle: 2.95 angstrom crystal structure of the dimeric form of penicillin2 binding protein 2 prime from enterococcus faecium Phyre2 PDB 2wad chain B Region: 127 - 663 Aligned: 504 Modelled: 521 Confidence: 100.0% Identity: 23% PDB header:peptide binding protein Chain: B: PDB Molecule:penicillin-binding protein 2b; PDBTitle: penicillin-binding protein 2b (pbp-2b) from streptococcus2 pneumoniae (strain 5204) Phyre2 PDB 4jbf chain B Region: 134 - 656 Aligned: 393 Modelled: 408 Confidence: 100.0% Identity: 30% PDB header:transferase Chain: B: PDB Molecule:peptidoglycan glycosyltransferase; PDBTitle: crystal structure of peptidoglycan glycosyltransferase from atopobium2 parvulum dsm 20469. Phyre2 PDB 3lo7 chain A Region: 134 - 660 Aligned: 395 Modelled: 422 Confidence: 100.0% Identity: 27% PDB header:transferase Chain: A: PDB Molecule:penicillin-binding protein a; PDBTitle: crystal structure of pbpa from mycobacterium tuberculosis Phyre2 1 c5u47A_ 2 c3oc2A_ 3 c4ye5A_ 4 c3pbqA_ 5 c6g9fA_ 6 c1pmdA_ 7 c3ue3A_ 8 c5lp4A_ 9 c3vslB_ 10 c5troA_ 11 c3pbnA_ 12 c1mwuA_ 13 c1qmfA_ 14 c3equB_ 15 c4ovdA_ 16 c4bjpA_ 17 c5dvyA_ 18 c2wadB_ 19 c4jbfB_ 20 c3lo7A_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210