1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	C	G	L	L	A	F	V	A	A	P	A	G	A	A	G	P	E	G	A	D	A	A	S	A	I	A	R	A	S	H	L	M	R	H	R	G	P	D	E	S	G	T	W	H	A	V	D	G	A	S	G	G	V	V	F	G	F	N	R
Secondary structure
SS confidence
Disorder		?	?										?	?	?	?	?	?	?	?	?	?	?													?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		L	S	I	I	D	I	A	H	S	H	Q	P	L	R	W	G	P	P	E	A	P	D	R	Y	V	L	V	F	N	G	E	I	Y	N	Y	L	E	L	R	D	E	L	R	T	Q	H	G	A	V	F	A	T	D	G	D	G	E	A	I	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		A	G	Y	H	H	W	G	T	E	V	L	Q	R	L	R	G	M	F	A	F	A	L	W	D	T	V	T	R	E	L	F	C	A	R	D	P	F	G	I	K	P	L	F	I	A	T	G	A	G	G	T	A	V	A	S	E	K	K	C	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		L	D	L	V	E	L	V	G	F	D	T	E	I	D	H	R	A	L	Q	H	Y	T	V	L	Q	Y	V	P	E	P	E	T	L	H	R	G	V	R	R	L	E	S	G	C	F	A	R	I	R	A	D	Q	L	A	P	V	I	T	R	Y
Secondary structure
SS confidence
Disorder								?																																														?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		F	V	P	R	F	A	A	S	P	I	T	N	D	N	D	Q	A	R	Y	D	E	I	T	A	V	L	E	D	S	V	A	K	H	M	R	A	D	V	T	V	G	A	F	L	S	G	G	I	D	S	T	A	I	A	A	L	A	I	R	H
Secondary structure
SS confidence
Disorder							?	?	?	?	?	?	?	?																																															?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		N	P	R	L	I	T	F	T	T	G	F	E	R	E	G	F	S	E	I	D	V	A	V	A	S	A	E	A	I	G	A	R	H	I	A	K	V	V	S	A	D	E	F	V	A	A	L	P	E	I	V	W	Y	L	D	E	P	V	A	D
Secondary structure
SS confidence
Disorder																		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		P	A	L	V	P	L	F	F	V	A	R	E	A	R	K	H	V	K	V	V	L	S	G	E	G	A	D	E	L	F	G	G	Y	T	I	Y	R	E	P	L	S	L	R	P	F	D	Y	L	P	K	P	L	R	R	S	M	G	K	V	S
Secondary structure
SS confidence
Disorder																																										?	?	?			?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		K	P	L	P	E	G	M	R	G	K	S	L	L	H	R	G	S	L	T	L	E	E	R	Y	Y	G	N	A	R	S	F	S	G	A	Q	L	R	E	V	L	P	G	F	R	P	D	W	T	H	T	D	V	T	A	P	V	Y	A	E	S
Secondary structure
SS confidence
Disorder						?	?	?							?		?		?	?											?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		A	G	W	D	P	V	A	R	M	Q	H	I	D	L	F	T	W	L	R	G	D	I	L	V	K	A	D	K	I	T	M	A	N	S	L	E	L	R	V	P	F	L	D	P	E	V	F	A	V	A	S	R	L	P	A	G	A	K	I	T
Secondary structure
SS confidence
Disorder				?
Disorder confidence
		.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560	.	.	.	.	.	.	.	.	.	570	.	.	.	.	.	.	.	.	.	580	.	.	.	.	.	.	.	.	.	590	.	.	.	.	.	.	.	.	.	600
Sequence		R	T	T	T	K	Y	A	L	R	R	A	L	E	P	I	V	P	A	H	V	L	H	R	P	K	L	G	F	P	V	P	I	R	H	W	L	R	A	G	E	L	L	E	W	A	Y	A	T	V	G	S	S	Q	A	G	H	L	V	D	I
Secondary structure
SS confidence
Disorder																																																						?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	610	.	.	.	.	.	.	.	.	.	620	.	.	.	.	.	.	.	.	.	630	.	.	.	.	.	.	.	.	.	640	.	.	.	.	.	.	.	.	.	650	.	.
Sequence		A	A	V	Y	R	M	L	D	E	H	R	C	G	S	S	D	H	S	R	R	L	W	T	M	L	I	F	M	L	W	H	A	I	F	V	E	H	S	V	V	P	Q	I	S	E	P	Q	Y	P	V	Q	L
Secondary structure
SS confidence
Disorder														?	?	?																								?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 9%) Alpha helix ( 46%) Beta strand ( 13%)

Rank Aligned region

PDB 1ct9 chain D Region: 2 - 651 Aligned: 492 Modelled: 523 Confidence: 100.0% Identity: 32% PDB header:ligase Chain: D: PDB Molecule:asparagine synthetase b; PDBTitle: crystal structure of asparagine synthetase b from2 escherichia coli Phyre2 PDB 1q15 chain A Region: 29 - 608 Aligned: 470 Modelled: 495 Confidence: 100.0% Identity: 20% PDB header:biosynthetic protein Chain: A: PDB Molecule:cara; PDBTitle: carbapenam synthetase Phyre2 PDB 1m1z chain B Region: 29 - 605 Aligned: 451 Modelled: 489 Confidence: 100.0% Identity: 27% PDB header:hydrolase Chain: B: PDB Molecule:beta-lactam synthetase; PDBTitle: beta-lactam synthetase apo enzyme Phyre2 PDB 1ct9 chain A domain 1 Region: 239 - 649 Aligned: 301 Modelled: 329 Confidence: 100.0% Identity: 29% Fold: Adenine nucleotide alpha hydrolase-like Superfamily: Adenine nucleotide alpha hydrolases-like Family: N-type ATP pyrophosphatases Phyre2 PDB 1jgt chain A domain 1 Region: 246 - 609 Aligned: 276 Modelled: 278 Confidence: 100.0% Identity: 28% Fold: Adenine nucleotide alpha hydrolase-like Superfamily: Adenine nucleotide alpha hydrolases-like Family: N-type ATP pyrophosphatases Phyre2 PDB 1q15 chain A domain 1 Region: 245 - 608 Aligned: 277 Modelled: 279 Confidence: 100.0% Identity: 26% Fold: Adenine nucleotide alpha hydrolase-like Superfamily: Adenine nucleotide alpha hydrolases-like Family: N-type ATP pyrophosphatases Phyre2 PDB 1ct9 chain A domain 2 Region: 2 - 242 Aligned: 191 Modelled: 211 Confidence: 100.0% Identity: 38% Fold: Ntn hydrolase-like Superfamily: N-terminal nucleophile aminohydrolases (Ntn hydrolases) Family: Class II glutamine amidotransferases Phyre2 PDB 1q15 chain A domain 2 Region: 29 - 238 Aligned: 187 Modelled: 206 Confidence: 100.0% Identity: 18% Fold: Ntn hydrolase-like Superfamily: N-terminal nucleophile aminohydrolases (Ntn hydrolases) Family: Class II glutamine amidotransferases Phyre2 PDB 1ecj chain B Region: 2 - 305 Aligned: 259 Modelled: 275 Confidence: 100.0% Identity: 23% PDB header:transferase Chain: B: PDB Molecule:glutamine phosphoribosylpyrophosphate PDBTitle: escherichia coli glutamine phosphoribosylpyrophosphate2 (prpp) amidotransferase complexed with 2 amp per tetramer Phyre2 PDB 1jgt chain A domain 2 Region: 1 - 242 Aligned: 195 Modelled: 216 Confidence: 100.0% Identity: 24% Fold: Ntn hydrolase-like Superfamily: N-terminal nucleophile aminohydrolases (Ntn hydrolases) Family: Class II glutamine amidotransferases Phyre2 PDB 1gph chain 1 Region: 2 - 304 Aligned: 257 Modelled: 265 Confidence: 100.0% Identity: 23% PDB header:transferase Chain: 1: PDB Molecule:glutamine phosphoribosyl-pyrophosphate amidotransferase; PDBTitle: structure of the allosteric regulatory enzyme of purine biosynthesis Phyre2 PDB 1gph chain 1 domain 2 Region: 2 - 237 Aligned: 194 Modelled: 207 Confidence: 100.0% Identity: 27% Fold: Ntn hydrolase-like Superfamily: N-terminal nucleophile aminohydrolases (Ntn hydrolases) Family: Class II glutamine amidotransferases Phyre2 PDB 1jxa chain A Region: 2 - 235 Aligned: 185 Modelled: 201 Confidence: 100.0% Identity: 30% PDB header:transferase Chain: A: PDB Molecule:glucosamine 6-phosphate synthase; PDBTitle: glucosamine 6-phosphate synthase with glucose 6-phosphate Phyre2 PDB 1ecf chain A domain 2 Region: 2 - 243 Aligned: 200 Modelled: 213 Confidence: 100.0% Identity: 26% Fold: Ntn hydrolase-like Superfamily: N-terminal nucleophile aminohydrolases (Ntn hydrolases) Family: Class II glutamine amidotransferases Phyre2 PDB 1xff chain A Region: 2 - 241 Aligned: 191 Modelled: 210 Confidence: 100.0% Identity: 28% Fold: Ntn hydrolase-like Superfamily: N-terminal nucleophile aminohydrolases (Ntn hydrolases) Family: Class II glutamine amidotransferases Phyre2 PDB 1ofd chain A domain 3 Region: 2 - 235 Aligned: 187 Modelled: 204 Confidence: 99.8% Identity: 29% Fold: Ntn hydrolase-like Superfamily: N-terminal nucleophile aminohydrolases (Ntn hydrolases) Family: Class II glutamine amidotransferases Phyre2 PDB 1te5 chain A Region: 2 - 236 Aligned: 211 Modelled: 224 Confidence: 99.8% Identity: 17% Fold: Ntn hydrolase-like Superfamily: N-terminal nucleophile aminohydrolases (Ntn hydrolases) Family: Class II glutamine amidotransferases Phyre2 PDB 1ea0 chain A domain 3 Region: 2 - 235 Aligned: 189 Modelled: 205 Confidence: 99.7% Identity: 29% Fold: Ntn hydrolase-like Superfamily: N-terminal nucleophile aminohydrolases (Ntn hydrolases) Family: Class II glutamine amidotransferases Phyre2 PDB 1xng chain A domain 1 Region: 253 - 566 Aligned: 234 Modelled: 238 Confidence: 99.7% Identity: 18% Fold: Adenine nucleotide alpha hydrolase-like Superfamily: Adenine nucleotide alpha hydrolases-like Family: N-type ATP pyrophosphatases Phyre2 PDB 4zfj chain B Region: 2 - 237 Aligned: 187 Modelled: 203 Confidence: 99.7% Identity: 22% PDB header:hydrolase Chain: B: PDB Molecule:amidohydrolase egtc; PDBTitle: ergothioneine-biosynthetic ntn hydrolase egtc, apo form Phyre2 1 c1ct9D_ 2 c1q15A_ 3 c1m1zB_ 4 d1ct9a1 5 d1jgta1 6 d1q15a1 7 d1ct9a2 8 d1q15a2 9 c1ecjB_ 10 d1jgta2 11 c1gph1_ 12 d1gph12 13 c1jxaA_ 14 d1ecfa2 15 d1xffa_ 16 d1ofda3 17 d1te5a_ 18 d1ea0a3 19 d1xnga1 20 c4zfjB_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127