1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	T	G	S	I	R	S	K	L	S	A	I	D	V	R	Q	L	G	T	V	D	Y	R	T	A	W	Q	L	Q	R	E	L	A	D	A	R	V	A	G	G	A	D	T	L	L	L	L	E	H	P	A	V	Y	T	A	G	R	R	T	E
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		T	H	E	R	P	I	D	G	T	P	V	V	D	T	D	R	G	G	K	I	T	W	H	G	P	G	Q	L	V	G	Y	P	I	I	G	L	A	E	P	L	D	V	V	N	Y	V	R	R	L	E	E	S	L	I	Q	V	C	A	D	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		G	L	H	A	G	R	V	D	G	R	S	G	V	W	L	P	G	R	P	A	R	K	V	A	A	I	G	V	R	V	S	R	A	T	T	L	H	G	F	A	L	N	C	D	C	D	L	A	A	F	T	A	I	V	P	C	G	I	S	D
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230
Sequence		A	A	V	T	S	L	S	A	E	L	G	R	T	V	T	V	D	E	V	R	A	T	V	A	A	A	V	C	A	A	L	D	G	V	L	P	V	G	D	R	V	P	S	H	A	V	P	S	P	L
Secondary structure
SS confidence
Disorder																																								?	?	?	?		?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 9%) Alpha helix ( 27%) Beta strand ( 29%)

Rank Aligned region

PDB 1w66 chain A domain 1 Region: 1 - 216 Aligned: 216 Modelled: 216 Confidence: 100.0% Identity: 99% Fold: Class II aaRS and biotin synthetases Superfamily: Class II aaRS and biotin synthetases Family: LplA-like Phyre2 PDB 2qhv chain A Region: 11 - 214 Aligned: 199 Modelled: 201 Confidence: 100.0% Identity: 43% PDB header:transferase Chain: A: PDB Molecule:lipoyltransferase; PDBTitle: structural basis of octanoic acid recognition by lipoate-protein2 ligase b Phyre2 PDB 2qhs chain A Region: 11 - 214 Aligned: 199 Modelled: 204 Confidence: 100.0% Identity: 43% PDB header:transferase Chain: A: PDB Molecule:lipoyltransferase; PDBTitle: structural basis of octanoic acid recognition by lipoate-protein2 ligase b Phyre2 PDB 1vqz chain A Region: 12 - 216 Aligned: 198 Modelled: 198 Confidence: 100.0% Identity: 21% PDB header:ligase Chain: A: PDB Molecule:lipoate-protein ligase, putative; PDBTitle: crystal structure of a putative lipoate-protein ligase a (sp_1160)2 from streptococcus pneumoniae tigr4 at 1.99 a resolution Phyre2 PDB 3r07 chain A Region: 9 - 224 Aligned: 210 Modelled: 216 Confidence: 100.0% Identity: 16% PDB header:transferase Chain: A: PDB Molecule:lipoate-protein ligase a subunit 1; PDBTitle: structural analysis of an archaeal lipoylation system. a bi-partite2 lipoate protein ligase and its e2 lipoyl domain from thermoplasma3 acidophilum Phyre2 PDB 1x2g chain B Region: 10 - 229 Aligned: 212 Modelled: 215 Confidence: 100.0% Identity: 19% PDB header:ligase Chain: B: PDB Molecule:lipoate-protein ligase a; PDBTitle: crystal structure of lipate-protein ligase a from2 escherichia coli Phyre2 PDB 2c8m chain A domain 1 Region: 9 - 224 Aligned: 205 Modelled: 216 Confidence: 100.0% Identity: 16% Fold: Class II aaRS and biotin synthetases Superfamily: Class II aaRS and biotin synthetases Family: LplA-like Phyre2 PDB 2e5a chain A Region: 13 - 229 Aligned: 209 Modelled: 217 Confidence: 100.0% Identity: 21% PDB header:ligase Chain: A: PDB Molecule:lipoyltransferase 1; PDBTitle: crystal structure of bovine lipoyltransferase in complex2 with lipoyl-amp Phyre2 PDB 1vqz chain A domain 2 Region: 13 - 216 Aligned: 197 Modelled: 198 Confidence: 100.0% Identity: 21% Fold: Class II aaRS and biotin synthetases Superfamily: Class II aaRS and biotin synthetases Family: LplA-like Phyre2 PDB 1x2g chain A domain 2 Region: 10 - 224 Aligned: 205 Modelled: 210 Confidence: 100.0% Identity: 17% Fold: Class II aaRS and biotin synthetases Superfamily: Class II aaRS and biotin synthetases Family: LplA-like Phyre2 PDB 2p5i chain A domain 1 Region: 11 - 224 Aligned: 210 Modelled: 211 Confidence: 100.0% Identity: 15% Fold: Class II aaRS and biotin synthetases Superfamily: Class II aaRS and biotin synthetases Family: LplA-like Phyre2 PDB 5ij6 chain A Region: 11 - 220 Aligned: 198 Modelled: 210 Confidence: 100.0% Identity: 15% PDB header:ligase,transferase Chain: A: PDB Molecule:lipoate--protein ligase; PDBTitle: crystal structure of enterococcus faecalis lipoate-protein ligase a2 (lpla-1) in complex with lipoic acid Phyre2 PDB 5iby chain A Region: 11 - 214 Aligned: 194 Modelled: 201 Confidence: 100.0% Identity: 18% PDB header:ligase,transferase Chain: A: PDB Molecule:lipoate--protein ligase; PDBTitle: crystal structure of enterococcus faecalis lipoate-protein ligase a2 (lpla-2) in complex with lipoic acid Phyre2 PDB 2p0l chain A domain 1 Region: 6 - 211 Aligned: 202 Modelled: 203 Confidence: 100.0% Identity: 14% Fold: Class II aaRS and biotin synthetases Superfamily: Class II aaRS and biotin synthetases Family: LplA-like Phyre2 PDB 5t8u chain A Region: 14 - 188 Aligned: 165 Modelled: 172 Confidence: 100.0% Identity: 15% PDB header:ligase Chain: A: PDB Molecule:lipoate-protein ligase 1; PDBTitle: crystal structure of p. falciparum lipl1 in complex lipoate Phyre2 PDB 2zgw chain A domain 2 Region: 74 - 211 Aligned: 122 Modelled: 138 Confidence: 99.0% Identity: 14% Fold: Class II aaRS and biotin synthetases Superfamily: Class II aaRS and biotin synthetases Family: Biotin holoenzyme synthetase Phyre2 PDB 2ej9 chain A Region: 74 - 211 Aligned: 125 Modelled: 138 Confidence: 98.7% Identity: 13% PDB header:ligase Chain: A: PDB Molecule:putative biotin ligase; PDBTitle: crystal structure of biotin protein ligase from2 methanococcus jannaschii Phyre2 PDB 2eay chain B Region: 84 - 211 Aligned: 117 Modelled: 128 Confidence: 98.6% Identity: 18% PDB header:ligase Chain: B: PDB Molecule:biotin [acetyl-coa-carboxylase] ligase; PDBTitle: crystal structure of biotin protein ligase from aquifex2 aeolicus Phyre2 PDB 2ewn chain A Region: 74 - 211 Aligned: 128 Modelled: 138 Confidence: 98.6% Identity: 21% PDB header:ligase, transcription Chain: A: PDB Molecule:bira bifunctional protein; PDBTitle: ecoli biotin repressor with co-repressor analog Phyre2 PDB 3bfm chain A Region: 74 - 212 Aligned: 129 Modelled: 139 Confidence: 98.3% Identity: 11% PDB header:unknown function Chain: A: PDB Molecule:biotin protein ligase-like protein of unknown function; PDBTitle: crystal structure of a biotin protein ligase-like protein of unknown2 function (tm1040_0394) from silicibacter sp. tm1040 at 1.70 a3 resolution Phyre2 1 d1w66a1 2 c2qhvA_ 3 c2qhsA_ 4 c1vqzA_ 5 c3r07A_ 6 c1x2gB_ 7 d2c8ma1 8 c2e5aA_ 9 d1vqza2 10 d1x2ga2 11 d2p5ia1 12 c5ij6A_ 13 c5ibyA_ 14 d2p0la1 15 c5t8uA_ 16 d2zgwa2 17 c2ej9A_ 18 c2eayB_ 19 c2ewnA_ 20 c3bfmA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56