1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	D	R	Q	K	E	F	V	L	R	T	L	E	E	R	D	I	R	F	V	R	L	W	F	T	D	V	L	G	F	L	K	S	V	A	I	A	P	A	E	L	E	G	A	F	E	E	G	I	G	F	D	G	S	S	I	E	G	F	A
Secondary structure
SS confidence
Disorder		?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		R	V	S	E	S	D	T	V	A	H	P	D	P	S	T	F	Q	V	L	P	W	A	T	S	S	G	H	H	H	S	A	R	M	F	C	D	I	T	M	P	D	G	S	P	S	W	A	D	P	R	H	V	L	R	R	Q	L	T	K	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		G	E	L	G	F	S	C	Y	V	H	P	E	I	E	F	F	L	L	K	P	G	P	E	D	G	S	V	P	V	P	V	D	N	A	G	Y	F	D	Q	A	V	H	D	S	A	L	N	F	R	R	H	A	I	D	A	L	E	F	M	G
Secondary structure
SS confidence
Disorder																								?	?	?	?							?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		I	S	V	E	F	S	H	H	E	G	A	P	G	Q	Q	E	I	D	L	R	F	A	D	A	L	S	M	A	D	N	V	M	T	F	R	Y	V	I	K	E	V	A	L	E	E	G	A	R	A	S	F	M	P	K	P	F	G	Q	H	P
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		G	S	A	M	H	T	H	M	S	L	F	E	G	D	V	N	A	F	H	S	A	D	D	P	L	Q	L	S	E	V	G	K	S	F	I	A	G	I	L	E	H	A	C	E	I	S	A	V	T	N	Q	W	V	N	S	Y	K	R	L	V
Secondary structure
SS confidence
Disorder																							?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		Q	G	G	E	A	P	T	A	A	S	W	G	A	A	N	R	S	A	L	V	R	V	P	M	Y	T	P	H	K	T	S	S	R	R	V	E	V	R	S	P	D	S	A	C	N	P	Y	L	T	F	A	V	L	L	A	A	G	L	R	G
Secondary structure
SS confidence
Disorder																										?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		V	E	K	G	Y	V	L	G	P	Q	A	E	D	N	V	W	D	L	T	P	E	E	R	R	A	M	G	Y	R	E	L	P	S	S	L	D	S	A	L	R	A	M	E	A	S	E	L	V	A	E	A	L	G	E	H	V	F	D	F	F
Secondary structure
SS confidence
Disorder																											?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.
Sequence		L	R	N	K	R	T	E	W	A	N	Y	R	S	H	V	T	P	Y	E	L	R	T	Y	L	S	L
Secondary structure
SS confidence
Disorder																										?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 6%) Alpha helix ( 37%) Beta strand ( 19%)

Rank Aligned region

PDB 1fpy chain E Region: 4 - 446 Aligned: 434 Modelled: 443 Confidence: 100.0% Identity: 38% PDB header:ligase Chain: E: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of glutamine synthetase from salmonella2 typhimurium with inhibitor phosphinothricin Phyre2 PDB 1hto chain B Region: 2 - 446 Aligned: 438 Modelled: 445 Confidence: 100.0% Identity: 31% PDB header:ligase Chain: B: PDB Molecule:glutamine synthetase; PDBTitle: crystallographic structure of a relaxed glutamine synthetase from2 mycobacterium tuberculosis Phyre2 PDB 3ng0 chain A Region: 3 - 445 Aligned: 429 Modelled: 443 Confidence: 100.0% Identity: 34% PDB header:ligase Chain: A: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of glutamine synthetase from synechocystis sp. pcc2 6803 Phyre2 PDB 5zlp chain H Region: 2 - 445 Aligned: 437 Modelled: 444 Confidence: 100.0% Identity: 32% PDB header:ligase Chain: H: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of glutamine synthetase from helicobacter pylori Phyre2 PDB 3qaj chain L Region: 5 - 446 Aligned: 434 Modelled: 442 Confidence: 100.0% Identity: 45% PDB header:ligase Chain: L: PDB Molecule:glutamine synthetase; PDBTitle: x-ray crystal structure of glutamine synthetase from bacillus subtilis2 cocrystallized with atp Phyre2 PDB 4s17 chain E Region: 3 - 446 Aligned: 412 Modelled: 417 Confidence: 100.0% Identity: 32% PDB header:ligase Chain: E: PDB Molecule:glutamine synthetase; PDBTitle: the crystal structure of glutamine synthetase from bifidobacterium2 adolescentis atcc 15703 Phyre2 PDB 4hpp chain A Region: 15 - 445 Aligned: 405 Modelled: 414 Confidence: 100.0% Identity: 27% PDB header:ligase Chain: A: PDB Molecule:probable glutamine synthetase; PDBTitle: crystal structure of novel glutamine synthase homolog Phyre2 PDB 2j9i chain L Region: 4 - 445 Aligned: 413 Modelled: 441 Confidence: 100.0% Identity: 26% PDB header:ligase Chain: L: PDB Molecule:glutamate-ammonia ligase domain-containing protein 1; PDBTitle: lengsin is a survivor of an ancient family of class i glutamine2 synthetases in eukaryotes that has undergone evolutionary re-3 engineering for a tissue-specific role in the vertebrate eye lens. Phyre2 PDB 5dm3 chain A Region: 4 - 445 Aligned: 372 Modelled: 407 Confidence: 100.0% Identity: 31% PDB header:ligase Chain: A: PDB Molecule:l-glutamine synthetase; PDBTitle: crystal structure of glutamine synthetase from chromohalobacter2 salexigens dsm 3043(csal_0679, target efi-550015) with bound adp Phyre2 PDB 1f52 chain A domain 2 Region: 106 - 446 Aligned: 336 Modelled: 341 Confidence: 100.0% Identity: 38% Fold: Glutamine synthetase/guanido kinase Superfamily: Glutamine synthetase/guanido kinase Family: Glutamine synthetase catalytic domain Phyre2 PDB 2bvc chain A domain 2 Region: 106 - 446 Aligned: 338 Modelled: 341 Confidence: 100.0% Identity: 33% Fold: Glutamine synthetase/guanido kinase Superfamily: Glutamine synthetase/guanido kinase Family: Glutamine synthetase catalytic domain Phyre2 PDB 2qc8 chain J Region: 6 - 358 Aligned: 335 Modelled: 353 Confidence: 100.0% Identity: 21% PDB header:ligase Chain: J: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of human glutamine synthetase in complex with adp2 and methionine sulfoximine phosphate Phyre2 PDB 4bax chain H Region: 17 - 363 Aligned: 326 Modelled: 347 Confidence: 100.0% Identity: 22% PDB header:ligase Chain: H: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of glutamine synthetase from streptomyces2 coelicolor Phyre2 PDB 2d3a chain J Region: 5 - 358 Aligned: 335 Modelled: 354 Confidence: 100.0% Identity: 21% PDB header:ligase Chain: J: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of the maize glutamine synthetase2 complexed with adp and methionine sulfoximine phosphate Phyre2 PDB 3fky chain D Region: 15 - 369 Aligned: 326 Modelled: 355 Confidence: 100.0% Identity: 22% PDB header:ligase Chain: D: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of the glutamine synthetase gln1deltan182 from the yeast saccharomyces cerevisiae Phyre2 PDB 4is4 chain G Region: 5 - 358 Aligned: 292 Modelled: 317 Confidence: 100.0% Identity: 22% PDB header:ligase Chain: G: PDB Molecule:glutamine synthetase; PDBTitle: the glutamine synthetase from the dicotyledonous plant m. truncatula2 is a decamer Phyre2 PDB 3o6x chain C Region: 106 - 431 Aligned: 322 Modelled: 325 Confidence: 100.0% Identity: 19% PDB header:ligase Chain: C: PDB Molecule:glutamine synthetase; PDBTitle: crystal structure of the type iii glutamine synthetase from2 bacteroides fragilis Phyre2 PDB 1f52 chain A domain 1 Region: 4 - 105 Aligned: 98 Modelled: 102 Confidence: 99.9% Identity: 40% Fold: beta-Grasp (ubiquitin-like) Superfamily: Glutamine synthetase, N-terminal domain Family: Glutamine synthetase, N-terminal domain Phyre2 PDB 2bvc chain A domain 1 Region: 4 - 105 Aligned: 98 Modelled: 102 Confidence: 99.9% Identity: 27% Fold: beta-Grasp (ubiquitin-like) Superfamily: Glutamine synthetase, N-terminal domain Family: Glutamine synthetase, N-terminal domain Phyre2 PDB 1tt4 chain B Region: 129 - 370 Aligned: 191 Modelled: 196 Confidence: 98.9% Identity: 21% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:putative cytoplasmic protein; PDBTitle: structure of np459575, a predicted glutathione synthase from2 salmonella typhimurium Phyre2 1 c1fpyE_ 2 c1htoB_ 3 c3ng0A_ 4 c5zlpH_ 5 c3qajL_ 6 c4s17E_ 7 c4hppA_ 8 c2j9iL_ 9 c5dm3A_ 10 d1f52a2 11 d2bvca2 12 c2qc8J_ 13 c4baxH_ 14 c2d3aJ_ 15 c3fkyD_ 16 c4is4G_ 17 c3o6xC_ 18 d1f52a1 19 d2bvca1 20 c1tt4B_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99