1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		L	H	I	A	P	L	I	S	Y	E	M	T	F	S	D	L	T	R	H	A	A	R	L	G	A	A	L	L	V	Y	Q	S	S	T	S	T	F	Q	G	S	W	A	Q	P	Q	L	A	A	Q	P	A	V	R	A	V	E	A	G	I	P
Secondary structure
SS confidence
Disorder		?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		A	V	H	A	S	L	S	G	D	S	S	A	F	D	T	R	G	R	R	L	A	W	C	S	A	E	F	N	G	A	I	V	V	N	V	P	L	A	S	N	V	T	L	Y	L	R	L	G	D	W	V	P	V	T	A	F	V	V	M	G
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140
Sequence		A	G	F	A	V	F	L	R	R	S	L	A	R	V	S	D	C	A	D	K
Secondary structure
SS confidence
Disorder										?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 11%) Alpha helix ( 38%) Beta strand ( 27%) TM helix ( 14%) (View membrane topology prediction)

Rank Aligned region

PDB 5vrh chain A Region: 1 - 126 Aligned: 126 Modelled: 126 Confidence: 100.0% Identity: 21% PDB header:transferase Chain: A: PDB Molecule:apolipoprotein n-acyltransferase; PDBTitle: apolipoprotein n-acyltransferase c387s active site mutant Phyre2 PDB 5n6m chain A Region: 1 - 130 Aligned: 129 Modelled: 130 Confidence: 100.0% Identity: 22% PDB header:membrane protein Chain: A: PDB Molecule:apolipoprotein n-acyltransferase; PDBTitle: structure of the membrane integral lipoprotein n-acyltransferase lnt2 from p. aeruginosa Phyre2 PDB 5h8l chain M Region: 1 - 97 Aligned: 97 Modelled: 97 Confidence: 99.9% Identity: 20% PDB header:hydrolase Chain: M: PDB Molecule:n-carbamoylputrescine amidohydrolase; PDBTitle: crystal structure of medicago truncatula n-carbamoylputrescine2 amidohydrolase (mtcpa) c158s mutant in complex with putrescine Phyre2 PDB 2plq chain A Region: 1 - 97 Aligned: 93 Modelled: 97 Confidence: 99.9% Identity: 25% PDB header:hydrolase Chain: A: PDB Molecule:aliphatic amidase; PDBTitle: crystal structure of the amidase from geobacillus pallidus rapc8 Phyre2 PDB 2e2k chain C Region: 1 - 97 Aligned: 93 Modelled: 97 Confidence: 99.9% Identity: 19% PDB header:hydrolase Chain: C: PDB Molecule:formamidase; PDBTitle: helicobacter pylori formamidase amif contains a fine-tuned cysteine-2 glutamate-lysine catalytic triad Phyre2 PDB 6i00 chain C Region: 2 - 99 Aligned: 91 Modelled: 98 Confidence: 99.9% Identity: 21% PDB header:hydrolase Chain: C: PDB Molecule:bifunctional nitrilase/nitrile hydratase nit4; PDBTitle: cryo-em informed directed evolution of nitrilase 4 leads to a change2 in quaternary structure. Phyre2 PDB 1uf5 chain A Region: 1 - 111 Aligned: 111 Modelled: 111 Confidence: 99.9% Identity: 13% Fold: Carbon-nitrogen hydrolase Superfamily: Carbon-nitrogen hydrolase Family: Carbamilase Phyre2 PDB 6mg6 chain D Region: 2 - 98 Aligned: 96 Modelled: 97 Confidence: 99.9% Identity: 20% PDB header:hydrolase Chain: D: PDB Molecule:carbon-nitrogen hydrolase; PDBTitle: crystal structure of carbon-nitrogen hydrolase from helicobacter2 pylori g27 Phyre2 PDB 1ems chain B Region: 2 - 97 Aligned: 92 Modelled: 96 Confidence: 99.9% Identity: 27% PDB header:antitumor protein Chain: B: PDB Molecule:nit-fragile histidine triad fusion protein; PDBTitle: crystal structure of the c. elegans nitfhit protein Phyre2 PDB 3wuy chain A Region: 2 - 97 Aligned: 95 Modelled: 93 Confidence: 99.9% Identity: 18% PDB header:hydrolase Chain: A: PDB Molecule:nitrilase; PDBTitle: crystal structure of nit6803 Phyre2 PDB 2vhh chain A Region: 2 - 98 Aligned: 94 Modelled: 97 Confidence: 99.9% Identity: 20% PDB header:hydrolase Chain: A: PDB Molecule:cg3027-pa; PDBTitle: crystal structure of a pyrimidine degrading enzyme from2 drosophila melanogaster Phyre2 PDB 1f89 chain A Region: 2 - 97 Aligned: 92 Modelled: 95 Confidence: 99.8% Identity: 27% Fold: Carbon-nitrogen hydrolase Superfamily: Carbon-nitrogen hydrolase Family: Nitrilase Phyre2 PDB 2w1v chain A Region: 1 - 97 Aligned: 93 Modelled: 97 Confidence: 99.8% Identity: 26% PDB header:hydrolase Chain: A: PDB Molecule:nitrilase homolog 2; PDBTitle: crystal structure of mouse nitrilase-2 at 1.4a resolution Phyre2 PDB 2vhi chain G Region: 2 - 98 Aligned: 94 Modelled: 97 Confidence: 99.8% Identity: 24% PDB header:hydrolase Chain: G: PDB Molecule:cg3027-pa; PDBTitle: crystal structure of a pyrimidine degrading enzyme from2 drosophila melanogaster Phyre2 PDB 1j31 chain A Region: 1 - 97 Aligned: 90 Modelled: 97 Confidence: 99.8% Identity: 20% Fold: Carbon-nitrogen hydrolase Superfamily: Carbon-nitrogen hydrolase Family: Carbamilase Phyre2 PDB 4hg3 chain C Region: 2 - 97 Aligned: 93 Modelled: 96 Confidence: 99.8% Identity: 25% PDB header:hydrolase Chain: C: PDB Molecule:probable hydrolase nit2; PDBTitle: structural insights into yeast nit2: wild-type yeast nit2 in complex2 with alpha-ketoglutarate Phyre2 PDB 3hkx chain A Region: 2 - 107 Aligned: 101 Modelled: 105 Confidence: 99.8% Identity: 26% PDB header:hydrolase Chain: A: PDB Molecule:amidase; PDBTitle: crystal structure analysis of an amidase from nesterenkonia sp. Phyre2 PDB 1ems chain A domain 2 Region: 2 - 97 Aligned: 92 Modelled: 96 Confidence: 99.8% Identity: 27% Fold: Carbon-nitrogen hydrolase Superfamily: Carbon-nitrogen hydrolase Family: Nitrilase Phyre2 PDB 5kha chain A Region: 2 - 100 Aligned: 97 Modelled: 99 Confidence: 99.8% Identity: 10% PDB header:ligase Chain: A: PDB Molecule:glutamine-dependent nad+ synthetase; PDBTitle: structure of glutamine-dependent nad+ synthetase from acinetobacter2 baumannii in complex with adenosine diphosphate (adp) Phyre2 PDB 4f4h chain A Region: 2 - 102 Aligned: 99 Modelled: 101 Confidence: 99.8% Identity: 20% PDB header:ligase Chain: A: PDB Molecule:glutamine dependent nad+ synthetase; PDBTitle: crystal structure of a glutamine dependent nad+ synthetase from2 burkholderia thailandensis Phyre2 1 c5vrhA_ 2 c5n6mA_ 3 c5h8lM_ 4 c2plqA_ 5 c2e2kC_ 6 c6i00C_ 7 d1uf5a_ 8 c6mg6D_ 9 c1emsB_ 10 c3wuyA_ 11 c2vhhA_ 12 d1f89a_ 13 c2w1vA_ 14 c2vhiG_ 15 d1j31a_ 16 c4hg3C_ 17 c3hkxA_ 18 d1emsa2 19 c5khaA_ 20 c4f4hA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99