1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	P	K	S	R	R	A	V	S	L	S	V	L	I	G	A	V	I	A	A	L	A	G	A	L	I	A	V	T	V	P	A	R	P	N	R	P	E	A	D	R	E	A	L	W	K	I	V	H	D	R	C	E	F	G	Y	R	R	T	G
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?																	?	?	?	?	?	?	?	?	?	?	?	?	?															?			?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		A	Y	A	P	C	T	F	V	D	E	Q	S	G	T	A	L	Y	K	A	D	F	D	P	Y	Q	F	L	L	I	P	L	A	R	I	T	G	I	E	D	P	A	L	R	E	S	A	G	R	N	Y	L	Y	D	A	W	A	A	R	F	L
Secondary structure
SS confidence
Disorder		?	?																																						?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		V	T	A	R	L	N	N	S	L	P	E	S	D	V	V	L	T	I	N	P	K	N	A	R	T	Q	D	Q	L	H	I	H	I	S	C	S	S	P	T	T	S	A	A	L	R	N	V	D	T	S	E	Y	V	G	W	K	Q	L	P	I
Secondary structure
SS confidence
Disorder						?			?	?		?										?		?	?	?	?																						?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		D	L	G	G	R	R	F	Q	G	L	A	V	D	T	K	A	F	E	S	R	N	L	F	R	D	I	Y	L	K	V	T	A	D	G	K	K	M	E	N	A	S	I	A	V	A	N	V	A	Q	D	Q	F	L	L	L	L	A	E	G	T
Secondary structure
SS confidence
Disorder																?	?	?	?	?	?									?	?	?	?	?	?	?	?	?										?											?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260
Sequence		E	D	Q	P	V	A	A	E	T	L	Q	D	H	D	C	S	I	T	K	S
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?							?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 30%) Alpha helix ( 39%) Beta strand ( 17%) TM helix ( 6%) (View membrane topology prediction)

Rank Aligned region

PDB 2pof chain A domain 1 Region: 41 - 258 Aligned: 218 Modelled: 218 Confidence: 100.0% Identity: 29% Fold: HIT-like Superfamily: HIT-like Family: CDH-like Phyre2 PDB 4q61 chain J Region: 52 - 153 Aligned: 95 Modelled: 102 Confidence: 96.7% Identity: 17% PDB header:cell cycle Chain: J: PDB Molecule:uncharacterized hit-like protein hp_0404; PDBTitle: hit like protein from helicobacter pylori 26695 Phyre2 PDB 4inc chain A Region: 64 - 153 Aligned: 84 Modelled: 90 Confidence: 96.5% Identity: 21% PDB header:hydrolase Chain: A: PDB Molecule:histidine triad nucleotide-binding protein 2, PDBTitle: human histidine triad nucleotide binding protein 2 Phyre2 PDB 3n1t chain E Region: 52 - 153 Aligned: 98 Modelled: 102 Confidence: 96.3% Identity: 12% PDB header:hydrolase Chain: E: PDB Molecule:hit-like protein hint; PDBTitle: crystal structure of the h101a mutant echint gmp complex Phyre2 PDB 1xqu chain A Region: 50 - 153 Aligned: 100 Modelled: 104 Confidence: 96.0% Identity: 19% Fold: HIT-like Superfamily: HIT-like Family: HIT (HINT, histidine triad) family of protein kinase-interacting proteins Phyre2 PDB 1xqu chain A Region: 50 - 153 Aligned: 100 Modelled: 104 Confidence: 96.0% Identity: 19% PDB header:hydrolase Chain: A: PDB Molecule:hit family hydrolase; PDBTitle: hit family hydrolase from clostridium thermocellum cth-393 Phyre2 PDB 1rzy chain A Region: 50 - 153 Aligned: 99 Modelled: 104 Confidence: 95.7% Identity: 15% Fold: HIT-like Superfamily: HIT-like Family: HIT (HINT, histidine triad) family of protein kinase-interacting proteins Phyre2 PDB 6d6j chain B Region: 52 - 153 Aligned: 98 Modelled: 102 Confidence: 95.6% Identity: 20% PDB header:hydrolase Chain: B: PDB Molecule:hit family hydrolase; PDBTitle: crystal structure of hit family hydrolase from legionella pneumophila2 philadelphia 1 Phyre2 PDB 3ano chain A Region: 44 - 153 Aligned: 103 Modelled: 109 Confidence: 95.5% Identity: 15% PDB header:transferase Chain: A: PDB Molecule:ap-4-a phosphorylase; PDBTitle: crystal structure of a novel diadenosine 5',5'''-p1,p4-tetraphosphate2 phosphorylase from mycobacterium tuberculosis h37rv Phyre2 PDB 3lb5 chain B Region: 49 - 153 Aligned: 100 Modelled: 105 Confidence: 95.5% Identity: 16% PDB header:cell cycle Chain: B: PDB Molecule:hit-like protein involved in cell-cycle regulation; PDBTitle: crystal structure of hit-like protein involved in cell-cycle2 regulation from bartonella henselae with unknown ligand Phyre2 PDB 6iq1 chain A Region: 52 - 153 Aligned: 99 Modelled: 102 Confidence: 95.4% Identity: 17% PDB header:hydrolase Chain: A: PDB Molecule:adenosine 5'-monophosphoramidase; PDBTitle: crystal structure of histidine triad nucleotide-binding protein from2 candida albicans Phyre2 PDB 1ems chain B Region: 64 - 153 Aligned: 84 Modelled: 90 Confidence: 95.3% Identity: 15% PDB header:antitumor protein Chain: B: PDB Molecule:nit-fragile histidine triad fusion protein; PDBTitle: crystal structure of the c. elegans nitfhit protein Phyre2 PDB 1ems chain A domain 1 Region: 59 - 153 Aligned: 89 Modelled: 95 Confidence: 95.3% Identity: 15% Fold: HIT-like Superfamily: HIT-like Family: HIT (HINT, histidine triad) family of protein kinase-interacting proteins Phyre2 PDB 1kpf chain A Region: 52 - 153 Aligned: 95 Modelled: 102 Confidence: 95.3% Identity: 16% Fold: HIT-like Superfamily: HIT-like Family: HIT (HINT, histidine triad) family of protein kinase-interacting proteins Phyre2 PDB 4egu chain A Region: 52 - 153 Aligned: 98 Modelled: 102 Confidence: 95.1% Identity: 17% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:histidine triad (hit) protein; PDBTitle: 0.95a resolution structure of a histidine triad protein from2 clostridium difficile Phyre2 PDB 4ndg chain B Region: 64 - 153 Aligned: 84 Modelled: 90 Confidence: 95.0% Identity: 15% PDB header:dna binding protein/rna/dna Chain: B: PDB Molecule:aprataxin; PDBTitle: human aprataxin (aptx) bound to rna-dna and zn - adenosine vanadate2 transition state mimic complex Phyre2 PDB 3oj7 chain A Region: 64 - 153 Aligned: 85 Modelled: 90 Confidence: 94.9% Identity: 21% PDB header:metal binding protein Chain: A: PDB Molecule:putative histidine triad family protein; PDBTitle: crystal structure of a histidine triad family protein from entamoeba2 histolytica, bound to sulfate Phyre2 PDB 1z84 chain A domain 2 Region: 49 - 153 Aligned: 95 Modelled: 105 Confidence: 94.8% Identity: 14% Fold: HIT-like Superfamily: HIT-like Family: Hexose-1-phosphate uridylyltransferase Phyre2 PDB 1vlr chain A domain 1 Region: 69 - 153 Aligned: 79 Modelled: 85 Confidence: 94.7% Identity: 16% Fold: HIT-like Superfamily: HIT-like Family: mRNA decapping enzyme DcpS C-terminal domain Phyre2 PDB 3l7x chain A Region: 53 - 153 Aligned: 97 Modelled: 101 Confidence: 94.6% Identity: 13% PDB header:cell cycle Chain: A: PDB Molecule:putative hit-like protein involved in cell-cycle PDBTitle: the crystal structure of smu.412c from streptococcus mutans ua159 Phyre2 1 d2pofa1 2 c4q61J_ 3 c4incA_ 4 c3n1tE_ 5 d1xqua_ 6 c1xquA_ 7 d1rzya_ 8 c6d6jB_ 9 c3anoA_ 10 c3lb5B_ 11 c6iq1A_ 12 c1emsB_ 13 d1emsa1 14 d1kpfa_ 15 c4eguA_ 16 c4ndgB_ 17 c3oj7A_ 18 d1z84a2 19 d1vlra1 20 c3l7xA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99