1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	R	A	G	R	W	G	P	G	M	T	G	L	D	P	A	E	F	L	S	L	V	E	A	A	A	L	A	P	S	A	D	N	R	R	E	V	Q	L	E	H	A	G	R	R	V	R	L	W	G	D	Q	T	W	R	S	A	P	E	H
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		R	R	I	M	S	L	V	A	I	G	A	A	V	E	N	V	K	L	R	A	G	R	L	G	F	E	T	K	V	C	W	F	P	D	S	G	N	P	G	L	V	A	E	I	D	V	D	R	L	P	Q	T	R	V	D	P	I	E	G	A
Secondary structure
SS confidence
Disorder																																																	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		I	E	R	R	R	T	N	R	R	V	R	F	R	G	P	P	L	S	Q	G	E	L	G	A	L	S	A	E	A	T	G	I	D	G	I	Q	L	H	W	F	D	S	P	E	T	R	K	Q	I	L	R	L	V	R	L	A	E	T	E	R
Secondary structure
SS confidence
Disorder										?	?	?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		F	R	S	R	E	L	H	E	E	L	F	S	A	V	R	F	D	I	G	W	T	A	S	S	D	D	G	L	P	P	G	S	L	E	V	E	A	W	M	R	P	M	F	R	G	L	R	H	W	R	V	L	R	L	L	R	T	V	G	M
Secondary structure
SS confidence
Disorder			?																	?	?	?	?	?	?		?	?						?	?	?	?		?	?	?				?		?		?		?	?	?					?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		H	H	A	L	G	L	R	A	A	Y	L	P	C	R	L	A	P	H	V	G	A	L	T	T	S	L	D	L	A	S	G	A	L	T	A	G	A	V	F	E	R	I	W	L	R	T	T	L	L	G	A	E	L	Q	P	F	A	A	S	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?		?	?																																														?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		V	L	S	L	P	A	C	E	W	V	A	P	H	V	R	A	A	L	V	G	G	W	N	L	L	A	P	G	H	W	P	M	M	V	F	R	I	G	H	A	R	A	P	S	V	R	T	M	R	Q	S	V	E	A	Y	C	Y	A	P	A
Secondary structure
SS confidence
Disorder			?	?		?	?	?	?	?													?		?		?																?	?	?	?													?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.
Sequence		E	R	S	G	S	D	S	E	S	R	F	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 25%) Alpha helix ( 45%) Beta strand ( 12%)

Rank Aligned region

PDB 2ymv chain A Region: 9 - 357 Aligned: 317 Modelled: 349 Confidence: 100.0% Identity: 22% PDB header:oxidoreductase Chain: A: PDB Molecule:acg nitroreductase; PDBTitle: structure of reduced m smegmatis 5246, a homologue of m.2 tuberculosis acg Phyre2 PDB 3gr3 chain B Region: 117 - 358 Aligned: 206 Modelled: 209 Confidence: 99.7% Identity: 18% PDB header:flavoprotein Chain: B: PDB Molecule:nitroreductase; PDBTitle: crystal structure of a nitroreductase-like family protein (pnba,2 bh06130) from bartonella henselae str. houston-1 at 1.45 a resolution Phyre2 PDB 3eo8 chain A Region: 117 - 356 Aligned: 206 Modelled: 222 Confidence: 99.7% Identity: 12% PDB header:flavoprotein Chain: A: PDB Molecule:blub-like flavoprotein; PDBTitle: crystal structure of blub-like flavoprotein (yp_001089088.1) from2 clostridium difficile 630 at 1.74 a resolution Phyre2 PDB 2isl chain B Region: 106 - 356 Aligned: 182 Modelled: 184 Confidence: 99.6% Identity: 21% PDB header:flavoprotein Chain: B: PDB Molecule:blub; PDBTitle: blub bound to reduced flavin (fmnh2) and molecular oxygen.2 (clear crystal form) Phyre2 PDB 2wzv chain B Region: 115 - 356 Aligned: 204 Modelled: 220 Confidence: 99.6% Identity: 18% PDB header:oxidoreductase Chain: B: PDB Molecule:nfnb protein; PDBTitle: crystal structure of the fmn-dependent nitroreductase nfnb2 from mycobacterium smegmatis Phyre2 PDB 4xom chain B Region: 116 - 357 Aligned: 175 Modelled: 190 Confidence: 99.6% Identity: 23% PDB header:unknown function Chain: B: PDB Molecule:coenzyme f420:l-glutamate ligase; PDBTitle: coenzyme f420:l-glutamate ligase (fbib) from mycobacterium2 tuberculosis (c-terminal domain). Phyre2 PDB 3e39 chain A Region: 113 - 356 Aligned: 156 Modelled: 171 Confidence: 99.5% Identity: 17% PDB header:oxidoreductase Chain: A: PDB Molecule:putative nitroreductase; PDBTitle: crystal structure of a putative nitroreductase in complex with fmn2 (dde_0787) from desulfovibrio desulfuricans subsp. at 1.70 a3 resolution Phyre2 PDB 3kwk chain A Region: 116 - 355 Aligned: 154 Modelled: 167 Confidence: 99.5% Identity: 15% PDB header:oxidoreductase Chain: A: PDB Molecule:putative nadh dehydrogenase/nad(p)h nitroreductase; PDBTitle: crystal structure of putative nadh dehydrogenase/nad(p)h2 nitroreductase (np_809094.1) from bacteroides thetaiotaomicron vpi-3 5482 at 1.54 a resolution Phyre2 PDB 3gh8 chain A Region: 114 - 358 Aligned: 181 Modelled: 184 Confidence: 99.5% Identity: 10% PDB header:oxidoreductase Chain: A: PDB Molecule:iodotyrosine dehalogenase 1; PDBTitle: crystal structure of mus musculus iodotyrosine deiodinase (iyd) bound2 to fmn and di-iodotyrosine (dit) Phyre2 PDB 5ko8 chain B Region: 116 - 357 Aligned: 177 Modelled: 180 Confidence: 99.5% Identity: 19% PDB header:oxidoreductase Chain: B: PDB Molecule:nitroreductase; PDBTitle: crystal structure of haliscomenobacter hydrossis iodotyrosine2 deiodinase (iyd) bound to fmn and mono-iodotyrosine (i-tyr) Phyre2 PDB 1vfr chain A Region: 116 - 356 Aligned: 198 Modelled: 199 Confidence: 99.5% Identity: 13% Fold: FMN-dependent nitroreductase-like Superfamily: FMN-dependent nitroreductase-like Family: NADH oxidase/flavin reductase Phyre2 PDB 3gfa chain B Region: 117 - 358 Aligned: 189 Modelled: 195 Confidence: 99.5% Identity: 12% PDB header:oxidoreductase Chain: B: PDB Molecule:putative nitroreductase; PDBTitle: crystal structure of a putative nitroreductase in complex with fmn2 (cd3205) from clostridium difficile 630 at 1.35 a resolution Phyre2 PDB 1bkj chain A Region: 116 - 356 Aligned: 152 Modelled: 167 Confidence: 99.5% Identity: 12% Fold: FMN-dependent nitroreductase-like Superfamily: FMN-dependent nitroreductase-like Family: NADH oxidase/flavin reductase Phyre2 PDB 3to0 chain A Region: 115 - 356 Aligned: 153 Modelled: 172 Confidence: 99.5% Identity: 13% PDB header:oxidoreductase Chain: A: PDB Molecule:iodotyrosine deiodinase 1; PDBTitle: crystal structure of mus musculus iodotyrosine deiodinase (iyd) c217a,2 c239a bound to fmn Phyre2 PDB 1nox chain A Region: 117 - 358 Aligned: 189 Modelled: 192 Confidence: 99.4% Identity: 16% Fold: FMN-dependent nitroreductase-like Superfamily: FMN-dependent nitroreductase-like Family: NADH oxidase/flavin reductase Phyre2 PDB 3n2s chain D Region: 117 - 356 Aligned: 151 Modelled: 166 Confidence: 99.4% Identity: 17% PDB header:oxidoreductase Chain: D: PDB Molecule:nadph-dependent nitro/flavin reductase; PDBTitle: structure of nfra1 nitroreductase from b. subtilis Phyre2 PDB 2i7h chain E Region: 117 - 356 Aligned: 164 Modelled: 167 Confidence: 99.4% Identity: 19% PDB header:oxidoreductase Chain: E: PDB Molecule:nitroreductase-like family protein; PDBTitle: crystal structure of the nitroreductase-like family protein from2 bacillus cereus Phyre2 PDB 3ek3 chain A Region: 115 - 356 Aligned: 176 Modelled: 186 Confidence: 99.4% Identity: 15% PDB header:flavoprotein Chain: A: PDB Molecule:nitroreductase; PDBTitle: crystal structure of nitroreductase with bound fmn (yp_211706.1) from2 bacteroides fragilis nctc 9343 at 1.70 a resolution Phyre2 PDB 5hdj chain A Region: 116 - 356 Aligned: 152 Modelled: 167 Confidence: 99.4% Identity: 14% PDB header:oxidoreductase Chain: A: PDB Molecule:nfra1; PDBTitle: structure of b. megaterium nfra1 Phyre2 PDB 4qly chain B Region: 117 - 356 Aligned: 153 Modelled: 154 Confidence: 99.4% Identity: 21% PDB header:oxidoreductase Chain: B: PDB Molecule:enone reductase cla-er; PDBTitle: crystal structure of cla-er, a novel enone reductase catalyzing a key2 step of a gut-bacterial fatty acid saturation metabolism,3 biohydrogenation Phyre2 1 c2ymvA_ 2 c3gr3B_ 3 c3eo8A_ 4 c2islB_ 5 c2wzvB_ 6 c4xomB_ 7 c3e39A_ 8 c3kwkA_ 9 c3gh8A_ 10 c5ko8B_ 11 d1vfra_ 12 c3gfaB_ 13 d1bkja_ 14 c3to0A_ 15 d1noxa_ 16 c3n2sD_ 17 c2i7hE_ 18 c3ek3A_ 19 c5hdjA_ 20 c4qlyB_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86