1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	G	R	I	S	D	R	D	I	A	A	I	R	E	G	A	R	I	E	D	V	V	G	D	Y	V	Q	L	R	R	A	G	A	D	S	L	K	G	L	C	P	F	H	N	E	K	S	P	S	F	H	V	R	P	N	H	G	H	F
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		H	C	F	G	C	G	E	G	G	D	V	Y	A	F	I	Q	K	I	E	H	V	S	F	V	E	A	V	E	L	L	A	D	R	I	G	H	T	I	S	Y	T	G	A	A	T	S	V	Q	R	D	R	G	S	R	S	R	L	L	A	A
Secondary structure
SS confidence
Disorder																																										?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		N	A	A	A	A	A	F	Y	A	Q	A	L	Q	S	D	E	A	A	P	A	R	Q	Y	L	T	E	R	S	F	D	A	A	A	A	R	K	F	G	C	G	F	A	P	S	G	W	D	S	L	T	K	H	L	Q	R	K	G	F	E	F
Secondary structure
SS confidence
Disorder																	?																																								?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		E	E	L	E	A	A	G	L	S	R	Q	G	R	H	G	P	M	D	R	F	H	R	R	L	L	W	P	I	R	T	S	A	G	E	V	V	G	F	G	A	R	R	L	F	D	D	D	A	M	E	A	K	Y	V	N	T	P	E	T	L
Secondary structure
SS confidence
Disorder																																														?	?		?												?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		L	Y	K	K	S	S	V	M	F	G	I	D	L	A	K	R	D	I	A	K	G	H	Q	A	V	V	V	E	G	Y	T	D	V	M	A	M	H	L	A	G	V	T	T	A	V	A	S	C	G	T	A	F	G	G	E	H	L	A	M	L
Secondary structure
SS confidence
Disorder		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		R	R	L	M	M	D	D	S	F	F	R	G	E	L	I	Y	V	F	D	G	D	E	A	G	R	A	A	A	L	K	A	F	D	G	E	Q	K	L	A	G	Q	S	F	V	A	V	A	P	D	G	M	D	P	C	D	L	R	L	K	C
Secondary structure
SS confidence
Disorder							?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		G	D	A	A	L	R	D	L	V	A	R	R	T	P	L	F	E	F	A	I	R	A	A	I	A	E	M	D	L	D	S	A	E	G	R	V	A	A	L	R	R	C	V	P	M	V	G	Q	I	K	D	P	T	L	R	D	E	Y	A	R
Secondary structure
SS confidence
Disorder																										?			?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		Q	L	A	G	W	V	G	W	A	D	V	A	Q	V	I	G	R	V	R	G	E	A	K	R	T	K	H	P	R	L	G	R	L	G	S	T	T	I	A	R	A	A	Q	R	P	T	A	G	P	P	T	E	L	A	V	R	P	D	P	R
Secondary structure
SS confidence
Disorder																				?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		D	P	T	L	W	P	Q	R	E	A	L	K	S	A	L	Q	Y	P	A	L	A	G	P	V	F	D	A	L	T	V	E	G	F	T	H	P	E	Y	A	A	V	R	A	A	I	D	T	A	G	G	T	S	A	G	L	S	G	A	Q	W
Secondary structure
SS confidence
Disorder		?	?	?	?																																															?		?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560	.	.	.	.	.	.	.	.	.	570	.	.	.	.	.	.	.	.	.	580	.	.	.	.	.	.	.	.	.	590	.	.	.	.	.	.	.	.	.	600
Sequence		L	D	M	V	R	Q	Q	T	T	S	T	V	T	S	A	L	I	S	E	L	G	V	E	A	I	Q	V	D	D	D	K	L	P	R	Y	I	A	G	V	L	A	R	L	Q	E	V	W	L	G	R	Q	I	A	E	V	K	S	K	L	Q
Secondary structure
SS confidence
Disorder																							?	?			?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	610	.	.	.	.	.	.	.	.	.	620	.	.	.	.	.	.	.	.	.	630	.	.	.	.	.	.	.	.	.
Sequence		R	M	S	P	I	E	Q	G	D	E	Y	H	A	L	F	G	D	L	V	A	M	E	A	Y	R	R	S	L	L	E	Q	A	S	G	D	D	L	T	A
Secondary structure
SS confidence
Disorder			?	?	?	?	?	?																							?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 17%) Alpha helix ( 52%) Beta strand ( 8%)

Rank Aligned region

PDB 2au3 chain A Region: 7 - 437 Aligned: 402 Modelled: 431 Confidence: 100.0% Identity: 31% PDB header:transferase Chain: A: PDB Molecule:dna primase; PDBTitle: crystal structure of the aquifex aeolicus primase (zinc binding and2 rna polymerase domains) Phyre2 PDB 5guj chain A Region: 112 - 441 Aligned: 320 Modelled: 320 Confidence: 100.0% Identity: 30% PDB header:transferase Chain: A: PDB Molecule:dna primase; PDBTitle: crystal structure of the bacillus subtilis dnag rna polymerase domain,2 natural degradation of full length dnag Phyre2 PDB 5w34 chain A Region: 112 - 429 Aligned: 316 Modelled: 318 Confidence: 100.0% Identity: 99% PDB header:transferase Chain: A: PDB Molecule:dna primase; PDBTitle: crystal structure of the rna polymerase domain (rpd) of mycobacterium2 tuberculosis primase dnag in complex with double-stranded dna3 gaccggaagtgg Phyre2 PDB 1dd9 chain A Region: 117 - 433 Aligned: 304 Modelled: 317 Confidence: 100.0% Identity: 34% Fold: DNA primase core Superfamily: DNA primase core Family: DNA primase DnaG catalytic core Phyre2 PDB 1dd9 chain A Region: 117 - 433 Aligned: 304 Modelled: 317 Confidence: 100.0% Identity: 34% PDB header:transferase Chain: A: PDB Molecule:dna primase; PDBTitle: structure of the dnag catalytic core Phyre2 PDB 4edv chain A Region: 110 - 437 Aligned: 318 Modelled: 325 Confidence: 100.0% Identity: 27% PDB header:transferase/transferase inhibitor Chain: A: PDB Molecule:dna primase; PDBTitle: the structure of the s. aureus dnag rna polymerase domain bound to2 pppgpp and manganese Phyre2 PDB 5vaz chain B Region: 117 - 437 Aligned: 311 Modelled: 321 Confidence: 100.0% Identity: 34% PDB header:transferase Chain: B: PDB Molecule:dna primase; PDBTitle: crystal structure of a dna primase domain from pseudomonas aeruginosa Phyre2 PDB 1nui chain A Region: 37 - 369 Aligned: 235 Modelled: 254 Confidence: 100.0% Identity: 24% PDB header:replication Chain: A: PDB Molecule:dna primase/helicase; PDBTitle: crystal structure of the primase fragment of bacteriophage t7 primase-2 helicase protein Phyre2 PDB 1d0q chain A Region: 3 - 102 Aligned: 99 Modelled: 100 Confidence: 99.9% Identity: 51% Fold: Rubredoxin-like Superfamily: Zinc beta-ribbon Family: DNA primase zinc finger Phyre2 PDB 1q57 chain G Region: 134 - 374 Aligned: 186 Modelled: 207 Confidence: 99.9% Identity: 27% PDB header:transferase Chain: G: PDB Molecule:dna primase/helicase; PDBTitle: the crystal structure of the bifunctional primase-helicase of2 bacteriophage t7 Phyre2 PDB 1nui chain A domain 1 Region: 135 - 369 Aligned: 183 Modelled: 197 Confidence: 99.8% Identity: 23% Fold: DNA primase core Superfamily: DNA primase core Family: Primase fragment of primase-helicase protein Phyre2 PDB 1t6t chain 1 Region: 248 - 368 Aligned: 106 Modelled: 121 Confidence: 99.6% Identity: 14% Fold: Toprim domain Superfamily: Toprim domain Family: Toprim domain Phyre2 PDB 5z51 chain A Region: 484 - 637 Aligned: 154 Modelled: 154 Confidence: 99.5% Identity: 98% PDB header:transferase Chain: A: PDB Molecule:dna primase; PDBTitle: helicase binding domain of primase from mycobacterium tuberculosis Phyre2 PDB 2r6c chain G Region: 480 - 623 Aligned: 136 Modelled: 144 Confidence: 98.9% Identity: 9% PDB header:replication Chain: G: PDB Molecule:dnag primase, helicase binding domain; PDBTitle: crystal form bh2 Phyre2 PDB 2lzn chain A Region: 478 - 625 Aligned: 141 Modelled: 148 Confidence: 98.7% Identity: 13% PDB header:transferase Chain: A: PDB Molecule:dna primase; PDBTitle: solution structure of s. aureus primase c-terminal domain Phyre2 PDB 2fcj chain A domain 1 Region: 258 - 335 Aligned: 72 Modelled: 78 Confidence: 98.7% Identity: 18% Fold: Toprim domain Superfamily: Toprim domain Family: Toprim domain Phyre2 PDB 2r5u chain D Region: 481 - 621 Aligned: 136 Modelled: 141 Confidence: 97.8% Identity: 11% PDB header:hydrolase Chain: D: PDB Molecule:replicative dna helicase; PDBTitle: crystal structure of the n-terminal domain of dnab helicase from2 mycobacterium tuberculosis Phyre2 PDB 4zc0 chain D Region: 481 - 623 Aligned: 132 Modelled: 143 Confidence: 97.6% Identity: 10% PDB header:hydrolase Chain: D: PDB Molecule:replicative dna helicase; PDBTitle: structure of a dodecameric bacterial helicase Phyre2 PDB 4im9 chain C Region: 488 - 617 Aligned: 125 Modelled: 130 Confidence: 97.5% Identity: 19% PDB header:transferase Chain: C: PDB Molecule:dna primase; PDBTitle: cystal structure of dnag primase c-terminal domain from vibrio2 cholerae Phyre2 PDB 6qel chain B Region: 478 - 621 Aligned: 144 Modelled: 144 Confidence: 97.4% Identity: 6% PDB header:replication Chain: B: PDB Molecule:replicative dna helicase; PDBTitle: e. coli dnabc apo complex Phyre2 1 c2au3A_ 2 c5gujA_ 3 c5w34A_ 4 d1dd9a_ 5 c1dd9A_ 6 c4edvA_ 7 c5vazB_ 8 c1nuiA_ 9 d1d0qa_ 10 c1q57G_ 11 d1nuia1 12 d1t6t1_ 13 c5z51A_ 14 c2r6cG_ 15 c2lznA_ 16 d2fcja1 17 c2r5uD_ 18 c4zc0D_ 19 c4im9C_ 20 c6qelB_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304 305 306 307 308 309 310 311 312 313 314 315 316 317 318 319