1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	P	P	K	A	A	D	G	R	R	P	S	P	D	G	G	L	G	G	F	V	P	F	P	A	D	R	A	A	S	Y	R	A	A	G	Y	W	S	G	R	T	L	D	T	V	L	S	D	A	A	R	R	W	P	D	R	L	A	V	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		D	A	G	D	R	P	G	H	G	G	L	S	Y	A	E	L	D	Q	R	A	D	R	A	A	A	A	L	H	G	L	G	I	T	P	G	D	R	V	L	L	Q	L	P	N	G	C	Q	F	A	V	A	L	F	A	L	L	R	A	G	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		I	P	V	M	C	L	P	G	H	R	A	A	E	L	G	H	F	A	A	V	S	A	A	T	G	L	V	V	A	D	V	A	S	G	F	D	Y	R	P	M	A	R	E	L	V	A	D	H	P	T	L	R	H	V	I	V	D	G	D	P
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		G	P	F	V	S	W	A	Q	L	C	A	Q	A	G	T	G	S	P	A	P	P	A	D	P	G	S	P	A	L	L	L	V	S	G	G	T	T	G	M	P	K	L	I	P	R	T	H	D	D	Y	V	F	N	A	T	A	S	A	A	L
Secondary structure
SS confidence
Disorder												?			?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		C	R	L	S	A	D	D	V	Y	L	V	V	L	A	A	G	H	N	F	P	L	A	C	P	G	L	L	G	A	M	T	V	G	A	T	A	V	F	A	P	D	P	S	P	E	A	A	F	A	A	I	E	R	H	G	V	T	V	T	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		L	V	P	A	L	A	K	L	W	A	Q	S	C	E	W	E	P	V	T	P	K	S	L	R	L	L	Q	V	G	G	S	K	L	E	P	E	D	A	R	R	V	R	T	A	L	T	P	G	L	Q	Q	V	F	G	M	A	E	G	L	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		N	F	T	R	I	G	D	P	P	E	V	V	E	H	T	Q	G	R	P	L	C	P	A	D	E	L	R	I	V	N	A	D	G	E	P	V	G	P	G	E	E	G	E	L	L	V	R	G	P	Y	T	L	N	G	Y	F	A	A	E	R
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		D	N	E	R	C	F	D	P	D	G	F	Y	R	S	G	D	L	V	R	R	R	D	D	G	N	L	V	V	T	G	R	V	K	D	V	I	C	R	A	G	E	T	I	A	A	S	D	L	E	E	Q	L	L	S	H	P	A	I	F	S
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		A	A	A	V	G	L	P	D	Q	Y	L	G	E	K	I	C	A	A	V	V	F	A	G	A	P	I	T	L	A	E	L	N	G	Y	L	D	R	R	G	V	A	A	H	T	R	P	D	Q	L	V	A	M	P	A	L	P	T	T	P	I
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560	.	.	.	.	.
Sequence		G	K	I	D	K	R	A	I	V	R	Q	L	G	I	A	T	G	P	V	T	T	Q	R	C	H
Secondary structure
SS confidence
Disorder															?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 5%) Alpha helix ( 35%) Beta strand ( 19%) TM helix ( 7%) (View membrane topology prediction)

Rank Aligned region

PDB 1mdb chain A Region: 17 - 558 Aligned: 534 Modelled: 542 Confidence: 100.0% Identity: 44% Fold: Acetyl-CoA synthetase-like Superfamily: Acetyl-CoA synthetase-like Family: Acetyl-CoA synthetase-like Phyre2 PDB 6eqo chain B Region: 43 - 555 Aligned: 512 Modelled: 512 Confidence: 100.0% Identity: 23% PDB header:oxidoreductase Chain: B: PDB Molecule:acetyl-coenzyme a synthetase; PDBTitle: tri-functional propionyl-coa synthase of erythrobacter sp. nap1 with2 bound nadp+ and phosphomethylphosphonic acid adenylate ester Phyre2 PDB 5wm7 chain A Region: 17 - 553 Aligned: 530 Modelled: 534 Confidence: 100.0% Identity: 54% PDB header:ligase Chain: A: PDB Molecule:salicylate-amp ligase; PDBTitle: crystal structure of cahj in complex with amp Phyre2 PDB 5es8 chain A Region: 13 - 550 Aligned: 499 Modelled: 509 Confidence: 100.0% Identity: 22% PDB header:ligase Chain: A: PDB Molecule:linear gramicidin synthetase subunit a; PDBTitle: crystal structure of the initiation module of lgra in the thiolation2 state Phyre2 PDB 5kei chain A Region: 17 - 552 Aligned: 519 Modelled: 536 Confidence: 100.0% Identity: 72% PDB header:ligase Chain: A: PDB Molecule:2,3-dihydroxybenzoate-amp ligase; PDBTitle: mycobacterium smegmatis mbta apo structure Phyre2 PDB 1pg4 chain A Region: 41 - 558 Aligned: 517 Modelled: 518 Confidence: 100.0% Identity: 24% Fold: Acetyl-CoA synthetase-like Superfamily: Acetyl-CoA synthetase-like Family: Acetyl-CoA synthetase-like Phyre2 PDB 5gxd chain A Region: 43 - 563 Aligned: 520 Modelled: 521 Confidence: 100.0% Identity: 20% PDB header:lyase Chain: A: PDB Molecule:amp-dependent synthetase and ligase; PDBTitle: structure of acryloyl-coa lyase prpe from dinoroseobacter shibae dfl2 12 Phyre2 PDB 6p1j chain A Region: 13 - 548 Aligned: 514 Modelled: 522 Confidence: 100.0% Identity: 24% PDB header:biosynthetic protein Chain: A: PDB Molecule:txo2; PDBTitle: the structure of condensation and adenylation domains of teixobactin-2 producing nonribosomal peptide synthetase txo2 serine module Phyre2 PDB 5ifi chain A Region: 41 - 555 Aligned: 510 Modelled: 515 Confidence: 100.0% Identity: 20% PDB header:ligase Chain: A: PDB Molecule:acetyl-coenzyme a synthetase; PDBTitle: crystal structure of acetyl-coa synthetase in complex with adenosine-2 5'-propylphosphate from cryptococcus neoformans h99 Phyre2 PDB 3rg2 chain H Region: 20 - 564 Aligned: 535 Modelled: 545 Confidence: 100.0% Identity: 39% PDB header:ligase Chain: H: PDB Molecule:enterobactin synthase component e (ente), 2,3-dihydro-2,3- PDBTitle: structure of a two-domain nrps fusion protein containing the ente2 adenylation domain and entb aryl-carrier protein from enterobactin3 biosynthesis Phyre2 PDB 1ry2 chain A Region: 41 - 554 Aligned: 512 Modelled: 514 Confidence: 100.0% Identity: 20% Fold: Acetyl-CoA synthetase-like Superfamily: Acetyl-CoA synthetase-like Family: Acetyl-CoA synthetase-like Phyre2 PDB 5u89 chain A Region: 37 - 563 Aligned: 491 Modelled: 506 Confidence: 100.0% Identity: 23% PDB header:hydrolase/inhibitor Chain: A: PDB Molecule:amino acid adenylation domain protein; PDBTitle: crystal structure of a cross-module fragment from the dimodular nrps2 dhbf Phyre2 PDB 4wd1 chain A Region: 41 - 552 Aligned: 504 Modelled: 512 Confidence: 100.0% Identity: 19% PDB header:ligase Chain: A: PDB Molecule:acetoacetate-coa ligase; PDBTitle: acetoacetyl-coa synthetase from streptomyces lividans Phyre2 PDB 5ja2 chain A Region: 13 - 559 Aligned: 521 Modelled: 527 Confidence: 100.0% Identity: 21% PDB header:ligase Chain: A: PDB Molecule:enterobactin synthase component f; PDBTitle: entf, a terminal nonribosomal peptide synthetase module bound to the2 non-native mbth-like protein pa2412 Phyre2 PDB 5wmm chain A Region: 41 - 548 Aligned: 467 Modelled: 475 Confidence: 100.0% Identity: 21% PDB header:biosynthetic protein Chain: A: PDB Molecule:nrps; PDBTitle: crystal structure of an adenylation domain interrupted by a2 methylation domain (ama4) from nonribosomal peptide synthetase tios Phyre2 PDB 4eat chain B Region: 37 - 552 Aligned: 507 Modelled: 516 Confidence: 100.0% Identity: 23% PDB header:ligase Chain: B: PDB Molecule:benzoate-coenzyme a ligase; PDBTitle: crystal structure of a benzoate coenzyme a ligase Phyre2 PDB 2vsq chain A Region: 13 - 562 Aligned: 523 Modelled: 533 Confidence: 100.0% Identity: 22% PDB header:ligase Chain: A: PDB Molecule:surfactin synthetase subunit 3; PDBTitle: structure of surfactin a synthetase c (srfa-c), a nonribosomal peptide2 synthetase termination module Phyre2 PDB 5ie2 chain A Region: 38 - 552 Aligned: 500 Modelled: 515 Confidence: 100.0% Identity: 24% PDB header:ligase Chain: A: PDB Molecule:oxalate--coa ligase; PDBTitle: crystal structure of a plant enzyme Phyre2 PDB 6n8e chain A Region: 13 - 550 Aligned: 512 Modelled: 522 Confidence: 100.0% Identity: 24% PDB header:hydrolase Chain: A: PDB Molecule:holo-obif1; PDBTitle: crystal structure of holo-obif1, a five domain nonribosomal peptide2 synthetase from burkholderia diffusa Phyre2 PDB 3cw9 chain A domain 1 Region: 40 - 553 Aligned: 498 Modelled: 514 Confidence: 100.0% Identity: 24% Fold: Acetyl-CoA synthetase-like Superfamily: Acetyl-CoA synthetase-like Family: Acetyl-CoA synthetase-like Phyre2 1 d1mdba_ 2 c6eqoB_ 3 c5wm7A_ 4 c5es8A_ 5 c5keiA_ 6 d1pg4a_ 7 c5gxdA_ 8 c6p1jA_ 9 c5ifiA_ 10 c3rg2H_ 11 d1ry2a_ 12 c5u89A_ 13 c4wd1A_ 14 c5ja2A_ 15 c5wmmA_ 16 c4eatB_ 17 c2vsqA_ 18 c5ie2A_ 19 c6n8eA_ 20 d3cw9a1 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152