1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	G	F	G	A	S	R	L	D	V	R	L	V	P	A	A	L	V	S	W	I	V	T	A	A	G	I	V	W	P	I	G	N	V	C	A	L	C	C	V	V	V	A	L	G	G	G	A	L	W	W	C	V	A	R	R	S	W	H	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?																																																		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		P	R	L	G	S	I	S	A	G	L	V	A	V	G	M	V	G	A	G	Y	G	L	A	V	A	L	R	S	E	A	V	D	R	H	P	I	T	V	A	F	G	T	S	A	L	V	T	V	T	P	S	E	S	P	V	S	L	G	R	G
Secondary structure
SS confidence
Disorder				?																												?	?	?				?	?	?												?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		R	L	M	F	R	A	T	V	Q	R	L	R	D	D	E	T	S	G	R	V	V	V	F	A	R	A	L	D	F	G	E	L	M	V	G	Q	P	V	Q	F	R	A	R	I	S	R	P	A	R	H	D	L	T	V	A	V	F	N	A	T
Secondary structure
SS confidence
Disorder												?	?	?	?	?	?	?												?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		G	R	P	T	V	G	R	A	G	P	V	H	R	A	A	H	I	V	R	H	R	F	A	A	A	V	R	E	V	L	P	A	D	Q	A	T	M	L	P	A	L	V	L	G	D	T	S	T	V	T	A	L	T	S	R	E	F	R	A	A
Secondary structure
SS confidence
Disorder		?		?	?		?																											?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		G	L	T	H	L	T	A	V	S	G	A	N	V	T	I	V	C	A	A	A	L	V	S	A	R	L	I	G	P	R	A	A	V	V	C	A	A	V	A	L	V	A	F	V	I	L	V	Q	P	T	A	S	V	L	R	A	A	V	M	G
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		A	I	A	L	V	G	M	L	S	A	R	R	R	Q	A	I	P	A	L	S	G	S	V	L	V	L	L	A	A	A	P	H	L	A	V	D	I	G	F	A	L	S	V	A	A	T	G	A	L	V	V	I	A	P	V	W	S	R	R	L
Secondary structure
SS confidence
Disorder												?	?	?																																														?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		V	D	R	G	C	P	K	V	L	A	D	A	L	A	V	A	A	A	A	Q	L	V	T	A	P	L	V	A	A	I	S	G	R	V	S	L	V	A	V	V	A	N	L	A	V	A	A	V	I	A	P	I	T	V	L	G	S	V	A	A
Secondary structure
SS confidence
Disorder		?	?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		V	L	V	V	P	W	P	A	G	A	Q	V	L	I	R	F	T	G	P	E	V	W	W	V	L	R	V	A	H	W	A	S	G	V	P	A	A	T	V	P	V	A	A	G	L	P	G	V	L	L	V	G	G	A	T	V	F	T	V	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.
Sequence		Q	W	R	W	R	W	F	R	A	A	M	C	K	T	M	A	V	A	V	I	C	L	L	A	W	S	L	S	G	L	V	G	P	S
Secondary structure
SS confidence
Disorder							?																				?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 12%) Alpha helix ( 66%) Beta strand ( 9%) TM helix ( 51%) (View membrane topology prediction)

Rank Aligned region

PDB 1ig3 chain A domain 2 Region: 203 - 242 Aligned: 40 Modelled: 40 Confidence: 57.4% Identity: 18% Fold: Thiamin pyrophosphokinase, catalytic domain Superfamily: Thiamin pyrophosphokinase, catalytic domain Family: Thiamin pyrophosphokinase, catalytic domain Phyre2 PDB 2hh9 chain A Region: 203 - 241 Aligned: 39 Modelled: 39 Confidence: 54.3% Identity: 15% PDB header:transferase Chain: A: PDB Molecule:thiamin pyrophosphokinase; PDBTitle: thiamin pyrophosphokinase from candida albicans Phyre2 PDB 2f17 chain A Region: 203 - 241 Aligned: 39 Modelled: 39 Confidence: 45.2% Identity: 18% PDB header:transferase Chain: A: PDB Molecule:thiamin pyrophosphokinase 1; PDBTitle: mouse thiamin pyrophosphokinase in a ternary complex with2 pyrithiamin pyrophosphate and amp at 2.5 angstrom Phyre2 PDB 3t98 chain A Region: 238 - 248 Aligned: 11 Modelled: 11 Confidence: 20.1% Identity: 36% PDB header:protein transport Chain: A: PDB Molecule:nuclear pore complex protein nup54; PDBTitle: molecular architecture of the transport channel of the nuclear pore2 complex: nup54/nup58 Phyre2 PDB 3t98 chain C Region: 227 - 248 Aligned: 22 Modelled: 22 Confidence: 19.8% Identity: 18% PDB header:protein transport Chain: C: PDB Molecule:nuclear pore complex protein nup54; PDBTitle: molecular architecture of the transport channel of the nuclear pore2 complex: nup54/nup58 Phyre2 PDB 1ig0 chain A Region: 203 - 242 Aligned: 40 Modelled: 40 Confidence: 18.3% Identity: 20% PDB header:transferase Chain: A: PDB Molecule:thiamin pyrophosphokinase; PDBTitle: crystal structure of yeast thiamin pyrophosphokinase Phyre2 PDB 3lm8 chain D Region: 214 - 242 Aligned: 29 Modelled: 29 Confidence: 18.0% Identity: 24% PDB header:transferase Chain: D: PDB Molecule:thiamine pyrophosphokinase; PDBTitle: crystal structure of thiamine pyrophosphokinase from bacillus2 subtilis, northeast structural genomics consortium target sr677 Phyre2 PDB 3cq9 chain C Region: 214 - 242 Aligned: 29 Modelled: 29 Confidence: 17.4% Identity: 21% PDB header:transferase Chain: C: PDB Molecule:uncharacterized protein lp_1622; PDBTitle: crystal structure of the lp_1622 protein from lactobacillus plantarum.2 northeast structural genomics consortium target lpr114 Phyre2 PDB 2k42 chain A Region: 224 - 243 Aligned: 20 Modelled: 20 Confidence: 17.4% Identity: 25% PDB header:signaling protein Chain: A: PDB Molecule:wiskott-aldrich syndrome protein; PDBTitle: solution structure of the gtpase binding domain of wasp in2 complex with espfu, an ehec effector Phyre2 PDB 1gwu chain A Region: 231 - 252 Aligned: 22 Modelled: 22 Confidence: 16.9% Identity: 36% Fold: Heme-dependent peroxidases Superfamily: Heme-dependent peroxidases Family: CCP-like Phyre2 PDB 1rp4 chain A Region: 248 - 255 Aligned: 8 Modelled: 8 Confidence: 15.6% Identity: 50% Fold: ERO1-like Superfamily: ERO1-like Family: ERO1-like Phyre2 PDB 2vza chain D Region: 220 - 238 Aligned: 19 Modelled: 19 Confidence: 15.5% Identity: 11% PDB header:cell adhesion Chain: D: PDB Molecule:cell filamentation protein; PDBTitle: type iv secretion system effector protein bepa Phyre2 PDB 2lnh chain A Region: 224 - 243 Aligned: 20 Modelled: 20 Confidence: 15.3% Identity: 20% PDB header:signaling protein/protein binding Chain: A: PDB Molecule:neural wiskott-aldrich syndrome protein; PDBTitle: enterohaemorrhagic e. coli (ehec) exploits a tryptophan switch to2 hijack host f-actin assembly Phyre2 PDB 3k94 chain A Region: 214 - 242 Aligned: 29 Modelled: 29 Confidence: 14.8% Identity: 21% PDB header:transferase Chain: A: PDB Molecule:thiamin pyrophosphokinase; PDBTitle: crystal structure of thiamin pyrophosphokinase from geobacillus2 thermodenitrificans, northeast structural genomics consortium target3 gtr2 Phyre2 PDB 3ahr chain A Region: 248 - 255 Aligned: 8 Modelled: 8 Confidence: 14.7% Identity: 38% PDB header:oxidoreductase Chain: A: PDB Molecule:ero1-like protein alpha; PDBTitle: inactive human ero1 Phyre2 PDB 3ihk chain C Region: 214 - 241 Aligned: 28 Modelled: 28 Confidence: 14.2% Identity: 21% PDB header:transferase Chain: C: PDB Molecule:thiamin pyrophosphokinase; PDBTitle: crystal structure of thiamin pyrophosphokinase from s.mutans,2 northeast structural genomics consortium target smr83 Phyre2 PDB 3mel chain C Region: 214 - 242 Aligned: 29 Modelled: 29 Confidence: 14.2% Identity: 14% PDB header:structural genomics, unknown function Chain: C: PDB Molecule:thiamin pyrophosphokinase family protein; PDBTitle: crystal structure of thiamin pyrophosphokinase family protein from2 enterococcus faecalis, northeast structural genomics consortium3 target efr150 Phyre2 PDB 1cee chain B Region: 224 - 243 Aligned: 20 Modelled: 20 Confidence: 13.9% Identity: 25% PDB header:structural protein regulation Chain: B: PDB Molecule:wiskott-aldrich syndrome protein wasp; PDBTitle: solution structure of cdc42 in complex with the gtpase2 binding domain of wasp Phyre2 PDB 3e0e chain A Region: 99 - 166 Aligned: 66 Modelled: 67 Confidence: 13.4% Identity: 15% PDB header:replication Chain: A: PDB Molecule:replication protein a; PDBTitle: crystal structure of a domain of replication protein a from2 methanococcus maripaludis. northeast structural genomics3 targe mrr110b Phyre2 PDB 1ig0 chain A domain 2 Region: 203 - 241 Aligned: 39 Modelled: 39 Confidence: 13.3% Identity: 21% Fold: Thiamin pyrophosphokinase, catalytic domain Superfamily: Thiamin pyrophosphokinase, catalytic domain Family: Thiamin pyrophosphokinase, catalytic domain Phyre2 1 d1ig3a2 2 c2hh9A_ 3 c2f17A_ 4 c3t98A_ 5 c3t98C_ 6 c1ig0A_ 7 c3lm8D_ 8 c3cq9C_ 9 c2k42A_ 10 d1gwua_ 11 d1rp4a_ 12 c2vzaD_ 13 c2lnhA_ 14 c3k94A_ 15 c3ahrA_ 16 c3ihkC_ 17 c3melC_ 18 c1ceeB_ 19 c3e0eA_ 20 d1ig0a2 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99