1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	A	H	G	K	K	R	R	G	H	R	S	S	G	V	A	A	G	V	T	G	P	A	S	C	L	H	S	V	H	S	H	R	L	A	S	G	V	E	T	H	P	P	N	R	H	E	S	A	S	I	W	N	R	R	R	V	L	L	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		N	S	T	Y	E	P	L	T	A	L	S	M	R	R	A	I	V	M	V	I	C	G	K	A	D	V	V	H	E	D	P	S	G	P	V	I	H	S	A	T	R	S	I	L	V	P	S	V	I	Q	L	R	S	Y	V	R	V	P	Y	R
Secondary structure
SS confidence
Disorder																																										?				?											?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		A	R	V	P	M	T	R	A	A	L	M	H	R	D	R	F	C	C	A	Y	C	G	G	K	A	D	T	V	D	H	V	V	P	R	S	R	G	G	A	H	S	W	E	N	C	V	A	C	C	S	P	C	N	H	R	K	G	D	R	L
Secondary structure
SS confidence
Disorder		?		?																																																					?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.
Sequence		L	T	E	L	G	W	A	L	R	R	A	P	L	P	P	T	G	P	H	W	R	L	L	S	A	V	K	E	L	D	P	S	W	A	R	Y	L	G	E	G	A	A
Secondary structure
SS confidence
Disorder																																							?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 29%) Alpha helix ( 34%) Beta strand ( 20%)

Rank Aligned region

PDB 5vgb chain A Region: 125 - 184 Aligned: 60 Modelled: 60 Confidence: 99.6% Identity: 27% PDB header:hydrolase/hydrolase inhibitor Chain: A: PDB Molecule:crispr-associated endonuclease cas9; PDBTitle: crystal structure of nmecas9 hnh domain bound to anti-crispr acriic1 Phyre2 PDB 2qgp chain A Region: 120 - 183 Aligned: 64 Modelled: 64 Confidence: 99.6% Identity: 36% PDB header:hydrolase Chain: A: PDB Molecule:hnh endonuclease; PDBTitle: x-ray structure of the nhn endonuclease from geobacter2 metallireducens. northeast structural genomics consortium target3 gmr87. Phyre2 PDB 5mkw chain A Region: 120 - 183 Aligned: 64 Modelled: 64 Confidence: 99.5% Identity: 28% PDB header:hydrolase Chain: A: PDB Molecule:dna annealing helicase and endonuclease zranb3; PDBTitle: crystal structure of the human zranb3 hnh domain Phyre2 PDB 5axw chain A Region: 127 - 184 Aligned: 58 Modelled: 58 Confidence: 99.2% Identity: 28% PDB header:hydrolase/rna/dna Chain: A: PDB Molecule:crispr-associated endonuclease cas9; PDBTitle: crystal structure of staphylococcus aureus cas9 in complex with sgrna2 and target dna (ttgggt pam) Phyre2 PDB 4oge chain A Region: 125 - 184 Aligned: 59 Modelled: 60 Confidence: 99.1% Identity: 29% PDB header:hydrolase Chain: A: PDB Molecule:hnh endonuclease domain protein; PDBTitle: crystal structure of the type ii-c cas9 enzyme from actinomyces2 naeslundii Phyre2 PDB 6ghc chain A Region: 122 - 176 Aligned: 55 Modelled: 55 Confidence: 99.0% Identity: 40% PDB header:hydrolase Chain: A: PDB Molecule:5-methylcytosine-specific restriction enzyme a; PDBTitle: modification dependent ecokmcra restriction endonuclease Phyre2 PDB 5h0m chain A Region: 127 - 185 Aligned: 59 Modelled: 59 Confidence: 99.0% Identity: 27% PDB header:hydrolase Chain: A: PDB Molecule:hnh endonuclease; PDBTitle: crystal structure of deep-sea thermophilic bacteriophage gve2 hnh2 endonuclease with zinc ion Phyre2 PDB 5x1h chain S Region: 127 - 175 Aligned: 47 Modelled: 49 Confidence: 98.7% Identity: 21% PDB header:protein transport Chain: S: PDB Molecule:icmj (dotn); PDBTitle: structure of legionella pneumophila dotn Phyre2 PDB 4cmq chain B Region: 127 - 184 Aligned: 55 Modelled: 58 Confidence: 98.3% Identity: 29% PDB header:hydrolase Chain: B: PDB Molecule:crispr-associated endonuclease cas9/csn1; PDBTitle: crystal structure of mn-bound s.pyogenes cas9 Phyre2 PDB 5zmm chain D Region: 122 - 176 Aligned: 55 Modelled: 55 Confidence: 97.9% Identity: 35% PDB header:dna binding protein Chain: D: PDB Molecule:uncharacterized protein mcra; PDBTitle: structure of the type iv phosphorothioation-dependent restriction2 endonuclease scomcra Phyre2 PDB 5g2x chain C Region: 126 - 173 Aligned: 38 Modelled: 47 Confidence: 90.9% Identity: 21% PDB header:transferase Chain: C: PDB Molecule:group ii intron-encoded protein ltra; PDBTitle: structure a of group ii intron complexed with its reverse2 transcriptase Phyre2 PDB 6ghs chain A Region: 114 - 173 Aligned: 60 Modelled: 60 Confidence: 88.4% Identity: 23% PDB header:hydrolase Chain: A: PDB Molecule:tagi restriction endonuclease; PDBTitle: modification dependent tagi restriction endonuclease Phyre2 PDB 3m7k chain A Region: 138 - 178 Aligned: 41 Modelled: 41 Confidence: 78.9% Identity: 29% PDB header:hydrolase/dna Chain: A: PDB Molecule:restriction endonuclease paci; PDBTitle: crystal structure of paci-dna enzyme product complex Phyre2 PDB 2gyk chain B domain 1 Region: 139 - 166 Aligned: 28 Modelled: 28 Confidence: 65.3% Identity: 29% Fold: His-Me finger endonucleases Superfamily: His-Me finger endonucleases Family: HNH-motif Phyre2 PDB 1ouo chain A Region: 139 - 179 Aligned: 41 Modelled: 41 Confidence: 51.3% Identity: 22% Fold: His-Me finger endonucleases Superfamily: His-Me finger endonucleases Family: Endonuclease I Phyre2 PDB 5b2o chain A Region: 147 - 180 Aligned: 24 Modelled: 34 Confidence: 46.3% Identity: 33% PDB header:hydrolase/rna/dna Chain: A: PDB Molecule:crispr-associated endonuclease cas9; PDBTitle: crystal structure of francisella novicida cas9 in complex with sgrna2 and target dna (tgg pam) Phyre2 PDB 1e7l chain A domain 2 Region: 127 - 150 Aligned: 24 Modelled: 24 Confidence: 44.8% Identity: 21% Fold: His-Me finger endonucleases Superfamily: His-Me finger endonucleases Family: Recombination endonuclease VII, N-terminal domain Phyre2 PDB 1xrz chain A Region: 136 - 147 Aligned: 12 Modelled: 12 Confidence: 33.5% Identity: 42% PDB header:transcription Chain: A: PDB Molecule:zinc finger y-chromosomal protein; PDBTitle: nmr structure of a zinc finger with cyclohexanylalanine2 substituted for the central aromatic residue Phyre2 PDB 2qnf chain B Region: 127 - 150 Aligned: 24 Modelled: 24 Confidence: 33.2% Identity: 21% PDB header:hydrolase/dna Chain: B: PDB Molecule:recombination endonuclease vii; PDBTitle: crystal structure of t4 endonuclease vii h43n mutant in2 complex with heteroduplex dna containing base mismatches Phyre2 PDB 7znf chain A Region: 136 - 141 Aligned: 6 Modelled: 6 Confidence: 27.5% Identity: 50% PDB header:zinc finger dna binding domain Chain: A: PDB Molecule:zinc finger; PDBTitle: alternating zinc fingers in the human male associated2 protein zfy: 2d nmr structure of an even finger and3 implications for "jumping-linker" dna recognition Phyre2 1 c5vgbA_ 2 c2qgpA_ 3 c5mkwA_ 4 c5axwA_ 5 c4ogeA_ 6 c6ghcA_ 7 c5h0mA_ 8 c5x1hS_ 9 c4cmqB_ 10 c5zmmD_ 11 c5g2xC_ 12 c6ghsA_ 13 c3m7kA_ 14 d2gykb1 15 d1ouoa_ 16 c5b2oA_ 17 d1e7la2 18 c1xrzA_ 19 c2qnfB_ 20 c7znfA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99