1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	A	Q	Y	D	P	V	L	L	S	V	D	K	H	V	A	L	I	T	V	N	D	P	D	R	R	N	A	V	T	D	E	M	S	A	Q	L	R	A	A	I	Q	R	A	E	G	D	P	D	V	H	A	V	V	V	T	G	A	G	K
Secondary structure
SS confidence
Disorder		?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		A	F	C	A	G	A	D	L	S	A	L	G	A	G	V	G	D	P	A	E	P	R	L	L	R	L	Y	D	G	F	M	A	V	S	S	C	N	L	P	T	I	A	A	V	N	G	A	A	V	G	A	G	L	N	L	A	L	A	A	D
Secondary structure
SS confidence
Disorder												?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		V	R	I	A	G	P	A	A	L	F	D	A	R	F	Q	K	L	G	L	H	P	G	G	G	A	T	W	M	L	Q	R	A	V	G	P	Q	V	A	R	A	A	L	L	F	G	M	C	F	D	A	E	S	A	V	R	H	G	L	A	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		M	V	A	D	D	P	V	T	A	A	L	E	L	A	A	G	P	A	A	A	P	R	E	V	V	L	A	S	K	A	T	M	R	A	T	A	S	P	G	S	L	D	L	E	Q	H	E	L	A	K	R	L	E	L	G	P	Q	A	K	S
Secondary structure
SS confidence
Disorder																																									?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.
Sequence		V	Q	S	P	E	F	A	A	R	L	A	A	A	Q	H	R
Secondary structure
SS confidence
Disorder			?												?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 7%) Alpha helix ( 57%) Beta strand ( 12%) TM helix ( 8%) (View membrane topology prediction)

Rank Aligned region

PDB 4jcs chain A Region: 1 - 256 Aligned: 250 Modelled: 256 Confidence: 100.0% Identity: 28% PDB header:lyase Chain: A: PDB Molecule:enoyl-coa hydratase/isomerase; PDBTitle: crystal structure of enoyl-coa hydratase/isomerase from cupriavidus2 metallidurans ch34 Phyre2 PDB 5z7r chain A Region: 1 - 256 Aligned: 249 Modelled: 256 Confidence: 100.0% Identity: 29% PDB header:lyase Chain: A: PDB Molecule:short-chain-enoyl-coa hydratase; PDBTitle: crystal strcuture of crotonase from clostridium acetobutylicum Phyre2 PDB 5jbx chain B Region: 3 - 256 Aligned: 248 Modelled: 254 Confidence: 100.0% Identity: 33% PDB header:lyase Chain: B: PDB Molecule:3-hydroxybutyryl-coa dehydratase; PDBTitle: crystal structure of liuc in complex with coenzyme a and malonic acid Phyre2 PDB 5zai chain B Region: 1 - 256 Aligned: 249 Modelled: 256 Confidence: 100.0% Identity: 27% PDB header:hydrolase Chain: B: PDB Molecule:3-hydroxypropionyl-coenzyme a dehydratase; PDBTitle: crystal strcuture of 3-hydroxypropionyl-coa dehydratase from2 metallosphaera sedula Phyre2 PDB 3hin chain A Region: 1 - 256 Aligned: 248 Modelled: 256 Confidence: 100.0% Identity: 26% PDB header:lyase Chain: A: PDB Molecule:putative 3-hydroxybutyryl-coa dehydratase; PDBTitle: crystal structure of putative enoyl-coa hydratase from2 rhodopseudomonas palustris cga009 Phyre2 PDB 3g64 chain A Region: 2 - 256 Aligned: 249 Modelled: 255 Confidence: 100.0% Identity: 31% PDB header:lyase Chain: A: PDB Molecule:putative enoyl-coa hydratase; PDBTitle: crystal structure of putative enoyl-coa hydratase from streptomyces2 coelicolor a3(2) Phyre2 PDB 4jfc chain A Region: 6 - 256 Aligned: 245 Modelled: 251 Confidence: 100.0% Identity: 29% PDB header:lyase Chain: A: PDB Molecule:enoyl-coa hydratase; PDBTitle: crystal structure of a enoyl-coa hydratase from polaromonas sp. js666 Phyre2 PDB 4og1 chain A Region: 1 - 256 Aligned: 246 Modelled: 252 Confidence: 100.0% Identity: 31% PDB header:lyase Chain: A: PDB Molecule:enoyl-coa hydratase/isomerase; PDBTitle: crystal structure of a putative enoyl-coa hydratase from2 novosphingobium aromaticivorans dsm 12444 Phyre2 PDB 3moy chain A Region: 2 - 256 Aligned: 247 Modelled: 255 Confidence: 100.0% Identity: 30% PDB header:lyase Chain: A: PDB Molecule:probable enoyl-coa hydratase; PDBTitle: crystal structure of probable enoyl-coa hydratase from mycobacterium2 smegmatis Phyre2 PDB 2qq3 chain F Region: 4 - 256 Aligned: 245 Modelled: 253 Confidence: 100.0% Identity: 29% PDB header:lyase Chain: F: PDB Molecule:enoyl-coa hydratase subunit i; PDBTitle: crystal structure of enoyl-coa hydrates subunit i (gk_2039)2 other form from geobacillus kaustophilus hta426 Phyre2 PDB 1nzy chain A Region: 4 - 256 Aligned: 247 Modelled: 253 Confidence: 100.0% Identity: 30% Fold: ClpP/crotonase Superfamily: ClpP/crotonase Family: Crotonase-like Phyre2 PDB 3h81 chain A Region: 3 - 256 Aligned: 246 Modelled: 254 Confidence: 100.0% Identity: 30% PDB header:lyase Chain: A: PDB Molecule:enoyl-coa hydratase echa8; PDBTitle: crystal structure of enoyl-coa hydratase from mycobacterium2 tuberculosis Phyre2 PDB 3i47 chain A Region: 3 - 256 Aligned: 248 Modelled: 249 Confidence: 100.0% Identity: 28% PDB header:lyase Chain: A: PDB Molecule:enoyl coa hydratase/isomerase (crotonase); PDBTitle: crystal structure of putative enoyl coa hydratase/isomerase2 (crotonase) from legionella pneumophila subsp. pneumophila str.3 philadelphia 1 Phyre2 PDB 4zu2 chain A Region: 5 - 256 Aligned: 245 Modelled: 252 Confidence: 100.0% Identity: 28% PDB header:hydrolase Chain: A: PDB Molecule:putative isohexenylglutaconyl-coa hydratase; PDBTitle: pseudomonas aeruginosa atue Phyre2 PDB 3r0o chain A Region: 1 - 256 Aligned: 251 Modelled: 256 Confidence: 100.0% Identity: 33% PDB header:lyase Chain: A: PDB Molecule:carnitinyl-coa dehydratase; PDBTitle: crystal structure of carnitinyl-coa hydratase from mycobacterium avium Phyre2 PDB 4k2n chain A Region: 1 - 256 Aligned: 248 Modelled: 256 Confidence: 100.0% Identity: 27% PDB header:isomerase Chain: A: PDB Molecule:enoyl-coa hydratase/carnithine racemase; PDBTitle: crystal structure of an enoyl-coa hydratase/ carnithine racemase from2 magnetospirillum magneticum Phyre2 PDB 3t88 chain A Region: 2 - 256 Aligned: 248 Modelled: 255 Confidence: 100.0% Identity: 25% PDB header:lyase/lyase inhibitor Chain: A: PDB Molecule:1,4-dihydroxy-2-naphthoyl-coa synthase; PDBTitle: crystal structure of escherichia coli menb in complex with substrate2 analogue, osb-ncoa Phyre2 PDB 2vx2 chain D Region: 5 - 256 Aligned: 245 Modelled: 252 Confidence: 100.0% Identity: 24% PDB header:isomerase Chain: D: PDB Molecule:enoyl-coa hydratase domain-containing protein 3; PDBTitle: crystal structure of human enoyl coenzyme a hydratase2 domain-containing protein 3 (echdc3) Phyre2 PDB 4mi2 chain C Region: 6 - 256 Aligned: 245 Modelled: 251 Confidence: 100.0% Identity: 33% PDB header:isomerase Chain: C: PDB Molecule:putative enoyl-coa hydratase/isomerase; PDBTitle: crystal structure of putative enoyl-coa hydratase/isomerase from2 mycobacterium abscessus Phyre2 PDB 2ej5 chain B Region: 1 - 256 Aligned: 241 Modelled: 256 Confidence: 100.0% Identity: 34% PDB header:lyase Chain: B: PDB Molecule:enoyl-coa hydratase subunit ii; PDBTitle: crystal structure of gk2038 protein (enoyl-coa hydratase subunit ii)2 from geobacillus kaustophilus Phyre2 1 c4jcsA_ 2 c5z7rA_ 3 c5jbxB_ 4 c5zaiB_ 5 c3hinA_ 6 c3g64A_ 7 c4jfcA_ 8 c4og1A_ 9 c3moyA_ 10 c2qq3F_ 11 d1nzya_ 12 c3h81A_ 13 c3i47A_ 14 c4zu2A_ 15 c3r0oA_ 16 c4k2nA_ 17 c3t88A_ 18 c2vx2D_ 19 c4mi2C_ 20 c2ej5B_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229