1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	G	E	D	S	I	R	S	F	P	V	P	D	L	G	E	G	L	Q	E	V	T	V	T	C	W	S	V	A	V	G	D	D	V	E	I	N	Q	T	L	C	S	V	E	T	A	K	A	E	V	E	I	P	S	P	Y	A	G	R
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		I	V	E	L	G	G	A	E	G	D	V	L	K	V	G	A	E	L	V	R	I	D	T	G	P	T	A	V	A	Q	P	N	G	E	G	A	V	P	T	L	V	G	Y	G	A	D	A	A	I	E	T	S	R	R	T	S	R	P	L	A
Secondary structure
SS confidence
Disorder																									?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		A	P	V	V	R	K	L	A	K	E	L	A	V	D	L	A	A	L	Q	R	G	S	G	A	G	G	V	I	T	R	A	D	V	L	A	A	A	R	G	G	V	G	A	G	P	D	V	R	P	V	H	G	V	H	A	R	M	A	E	K
Secondary structure
SS confidence
Disorder		?	?	?		?	?					?	?	?	?				?	?	?	?	?	?	?	?	?	?	?								?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		M	T	L	S	H	K	E	I	P	T	A	K	A	S	V	E	V	I	C	A	E	L	L	R	L	R	D	R	F	V	S	A	A	P	E	I	T	P	F	A	L	T	L	R	L	L	V	I	A	L	K	H	N	V	I	L	N	S	T	W
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		V	D	S	G	E	G	P	Q	V	H	V	H	R	G	V	H	L	G	F	G	A	A	T	E	R	G	L	L	V	P	V	V	T	D	A	Q	D	K	N	T	R	E	L	A	S	R	V	A	E	L	I	T	G	A	R	E	G	T	L	T
Secondary structure
SS confidence
Disorder																																																									?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		P	A	E	L	R	G	S	T	F	T	V	S	N	F	G	A	L	G	V	D	D	G	V	P	V	I	N	H	P	E	A	A	I	L	G	L	G	A	I	K	P	R	P	V	V	V	G	G	E	V	V	A	R	P	T	M	T	L	T	C
Secondary structure
SS confidence
Disorder					?	?	?											?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.
Sequence		V	F	D	H	R	V	V	D	G	A	Q	V	A	Q	F	M	C	E	L	R	D	L	I	E	S	P	E	T	A	L	L	D	L
Secondary structure
SS confidence
Disorder																																?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 22%) Alpha helix ( 27%) Beta strand ( 31%)

Rank Aligned region

PDB 3l60 chain A Region: 165 - 393 Aligned: 229 Modelled: 229 Confidence: 100.0% Identity: 100% PDB header:oxidoreductase Chain: A: PDB Molecule:branched-chain alpha-keto acid dehydrogenase; PDBTitle: crystal structure of branched-chain alpha-keto acid dehydrogenase2 subunit e2 from mycobacterium tuberculosis Phyre2 PDB 2ii4 chain C Region: 164 - 393 Aligned: 227 Modelled: 230 Confidence: 100.0% Identity: 32% PDB header:transferase Chain: C: PDB Molecule:lipoamide acyltransferase component of branched-chain PDBTitle: crystal structure of a cubic core of the dihydrolipoamide2 acyltransferase (e2b) component in the branched-chain alpha-ketoacid3 dehydrogenase complex (bckdc), coenzyme a-bound form Phyre2 PDB 1b5s chain A Region: 157 - 391 Aligned: 233 Modelled: 235 Confidence: 100.0% Identity: 33% Fold: CoA-dependent acyltransferases Superfamily: CoA-dependent acyltransferases Family: CAT-like Phyre2 PDB 1dpb chain A Region: 155 - 391 Aligned: 235 Modelled: 237 Confidence: 100.0% Identity: 29% Fold: CoA-dependent acyltransferases Superfamily: CoA-dependent acyltransferases Family: CAT-like Phyre2 PDB 4n72 chain B Region: 157 - 389 Aligned: 231 Modelled: 233 Confidence: 100.0% Identity: 28% PDB header:transferase Chain: B: PDB Molecule:pyruvate dehydrogenase (dihydrolipoyltransacetylase PDBTitle: catalytic domain from dihydrolipoamide acetyltransferase of pyruvate2 dehydrogenase from escherichia coli Phyre2 PDB 3b8k chain A Region: 155 - 391 Aligned: 233 Modelled: 233 Confidence: 100.0% Identity: 30% PDB header:transferase Chain: A: PDB Molecule:dihydrolipoyllysine-residue acetyltransferase; PDBTitle: structure of the truncated human dihydrolipoyl acetyltransferase (e2) Phyre2 PDB 1scz chain A Region: 162 - 393 Aligned: 228 Modelled: 232 Confidence: 100.0% Identity: 29% Fold: CoA-dependent acyltransferases Superfamily: CoA-dependent acyltransferases Family: CAT-like Phyre2 PDB 6h60 chain A Region: 159 - 389 Aligned: 227 Modelled: 231 Confidence: 100.0% Identity: 27% PDB header:oxidoreductase Chain: A: PDB Molecule:pyruvate dehydrogenase protein x component, mitochondrial; PDBTitle: pseudo-atomic structural model of the e3bp component of the human2 pyruvate dehydrogenase multienzyme complex Phyre2 PDB 3mae chain A Region: 162 - 388 Aligned: 223 Modelled: 227 Confidence: 100.0% Identity: 33% PDB header:transferase Chain: A: PDB Molecule:2-oxoisovalerate dehydrogenase e2 component, PDBTitle: crystal structure of probable dihydrolipoamide acetyltransferase from2 listeria monocytogenes 4b f2365 Phyre2 PDB 3rqc chain B Region: 164 - 390 Aligned: 217 Modelled: 227 Confidence: 100.0% Identity: 29% PDB header:transferase Chain: B: PDB Molecule:probable lipoamide acyltransferase; PDBTitle: crystal structure of the catalytic core of the 2-oxoacid dehydrogenase2 multienzyme complex from thermoplasma acidophilum Phyre2 PDB 1q23 chain A Region: 184 - 386 Aligned: 186 Modelled: 203 Confidence: 100.0% Identity: 15% Fold: CoA-dependent acyltransferases Superfamily: CoA-dependent acyltransferases Family: CAT-like Phyre2 PDB 3cla chain A Region: 184 - 386 Aligned: 189 Modelled: 203 Confidence: 100.0% Identity: 14% Fold: CoA-dependent acyltransferases Superfamily: CoA-dependent acyltransferases Family: CAT-like Phyre2 PDB 2i9d chain C Region: 184 - 384 Aligned: 168 Modelled: 201 Confidence: 100.0% Identity: 16% PDB header:transferase Chain: C: PDB Molecule:chloramphenicol acetyltransferase; PDBTitle: chloramphenicol acetyltransferase Phyre2 PDB 2dne chain A Region: 2 - 106 Aligned: 105 Modelled: 105 Confidence: 99.9% Identity: 22% PDB header:transferase Chain: A: PDB Molecule:dihydrolipoyllysine-residue acetyltransferase PDBTitle: solution structure of rsgi ruh-058, a lipoyl domain of2 human 2-oxoacid dehydrogenase Phyre2 PDB 2dnc chain A Region: 2 - 96 Aligned: 95 Modelled: 95 Confidence: 99.9% Identity: 22% PDB header:transferase Chain: A: PDB Molecule:pyruvate dehydrogenase protein x component; PDBTitle: solution structure of rsgi ruh-054, a lipoyl domain from2 human 2-oxoacid dehydrogenase Phyre2 PDB 1y8o chain B domain 1 Region: 6 - 94 Aligned: 89 Modelled: 89 Confidence: 99.9% Identity: 20% Fold: Barrel-sandwich hybrid Superfamily: Single hybrid motif Family: Biotinyl/lipoyl-carrier proteins and domains Phyre2 PDB 2q8i chain B Region: 6 - 93 Aligned: 88 Modelled: 88 Confidence: 99.9% Identity: 20% PDB header:transferase Chain: B: PDB Molecule:dihydrolipoyllysine-residue acetyltransferase component of PDBTitle: pyruvate dehydrogenase kinase isoform 3 in complex with antitumor drug2 radicicol Phyre2 PDB 2l5t chain A Region: 7 - 83 Aligned: 77 Modelled: 77 Confidence: 99.9% Identity: 45% PDB header:transferase Chain: A: PDB Molecule:lipoamide acyltransferase; PDBTitle: solution nmr structure of e2 lipoyl domain from thermoplasma2 acidophilum Phyre2 PDB 1k8m chain A Region: 5 - 90 Aligned: 86 Modelled: 86 Confidence: 99.9% Identity: 37% Fold: Barrel-sandwich hybrid Superfamily: Single hybrid motif Family: Biotinyl/lipoyl-carrier proteins and domains Phyre2 PDB 1ghj chain A Region: 8 - 85 Aligned: 78 Modelled: 78 Confidence: 99.9% Identity: 26% Fold: Barrel-sandwich hybrid Superfamily: Single hybrid motif Family: Biotinyl/lipoyl-carrier proteins and domains Phyre2 1 c3l60A_ 2 c2ii4C_ 3 d1b5sa_ 4 d1dpba_ 5 c4n72B_ 6 c3b8kA_ 7 d1scza_ 8 c6h60A_ 9 c3maeA_ 10 c3rqcB_ 11 d1q23a_ 12 d3claa_ 13 c2i9dC_ 14 c2dneA_ 15 c2dncA_ 16 d1y8ob1 17 c2q8iB_ 18 c2l5tA_ 19 d1k8ma_ 20 d1ghja_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194