1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	T	T	P	S	I	A	I	A	P	S	F	A	D	E	H	R	R	L	V	A	E	L	N	N	K	L	A	A	A	A	L	G	G	N	E	R	A	R	K	R	H	V	S	R	G	K	L	L	P	R	E	R	V	D	R	L	L	D	P
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?																			?	?	?	?	?									?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		G	S	P	F	L	E	L	A	P	L	A	A	G	G	M	Y	G	D	E	S	P	G	A	G	I	I	T	G	I	G	R	V	S	G	R	Q	C	V	I	V	A	N	D	A	T	V	K	G	G	T	Y	Y	P	M	T	V	K	K	H	L
Secondary structure
SS confidence
Disorder															?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		R	A	Q	E	V	A	L	Q	N	M	L	P	C	I	Y	L	V	D	S	G	G	A	F	L	P	R	Q	D	E	V	F	P	D	R	E	H	F	G	R	I	F	Y	N	Q	A	T	M	S	A	K	G	I	P	Q	V	A	A	V	L	G
Secondary structure
SS confidence
Disorder																					?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		S	C	T	A	G	G	A	Y	V	P	A	M	S	D	E	A	V	I	V	R	E	Q	G	T	I	F	L	G	G	P	P	L	V	K	A	A	T	G	E	I	V	S	A	E	E	L	G	G	G	D	L	H	S	R	T	S	G	V	T	D
Secondary structure
SS confidence
Disorder																																								?				?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		H	L	A	D	D	D	E	D	A	L	R	I	V	R	A	I	A	D	T	F	G	P	C	E	P	A	Q	W	D	V	R	R	S	V	E	P	K	Y	P	Q	A	E	L	Y	D	V	V	P	P	D	P	R	V	P	Y	D	V	H	E	V
Secondary structure
SS confidence
Disorder																										?	?				?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		V	V	R	I	V	D	G	S	E	F	S	E	F	K	A	K	Y	G	K	T	L	V	T	A	F	A	R	V	H	G	H	P	V	G	I	V	A	N	N	G	V	L	F	S	E	S	A	L	K	G	A	H	F	I	E	L	C	D	K	R
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		K	I	P	L	L	F	L	Q	N	I	A	G	F	M	V	G	R	D	Y	E	A	G	G	I	A	K	H	G	A	K	M	V	T	A	V	A	C	A	R	V	P	K	L	T	V	V	I	G	G	S	Y	G	A	G	N	Y	S	M	C	G
Secondary structure
SS confidence
Disorder												?	?	?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		R	A	Y	S	P	R	F	L	W	M	W	P	N	A	R	I	S	V	M	G	G	E	Q	A	A	S	V	L	A	T	V	R	G	E	Q	L	S	A	A	G	T	P	W	S	P	D	E	E	E	A	F	K	A	P	I	R	A	Q	Y	E
Secondary structure
SS confidence
Disorder																																?	?	?	?		?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.
Sequence		D	Q	G	N	P	Y	Y	S	T	A	R	L	W	D	D	G	I	I	D	P	A	D	T	R	T	V	V	G	L	A	L	S	L	C	A	H	A	P	L	D	Q	V	G	Y	G	V	F	R	M
Secondary structure
SS confidence
Disorder																																					?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 16%) Alpha helix ( 46%) Beta strand ( 16%) TM helix ( 11%) (View membrane topology prediction)

Rank Aligned region

PDB 4q0g chain C Region: 10 - 529 Aligned: 520 Modelled: 520 Confidence: 100.0% Identity: 100% PDB header:ligase Chain: C: PDB Molecule:probable acetyl-/propionyl-coa carboxylase (beta subunit) PDBTitle: crystal structure of beta subunit of acyl-coa carboxylase accd1 from2 mycobacterium tuberculosis Phyre2 PDB 3u9r chain B Region: 2 - 529 Aligned: 528 Modelled: 528 Confidence: 100.0% Identity: 66% PDB header:ligase Chain: B: PDB Molecule:methylcrotonyl-coa carboxylase, beta-subunit; PDBTitle: crystal structure of p. aeruginosa 3-methylcrotonyl-coa carboxylase2 (mcc), beta subunit Phyre2 PDB 5ikl chain D Region: 2 - 528 Aligned: 502 Modelled: 524 Confidence: 100.0% Identity: 47% PDB header:ligase Chain: D: PDB Molecule:geranyl-coa carboxylase, beta-subunit; PDBTitle: crystal structure of p. aeruginosa geranyl-coa carboxylase (gcc), beta2 subunit Phyre2 PDB 1pix chain B Region: 1 - 529 Aligned: 521 Modelled: 529 Confidence: 100.0% Identity: 25% PDB header:lyase Chain: B: PDB Molecule:glutaconyl-coa decarboxylase a subunit; PDBTitle: crystal structure of the carboxyltransferase subunit of the2 bacterial ion pump glutaconyl-coenzyme a decarboxylase Phyre2 PDB 3glm chain D Region: 1 - 527 Aligned: 513 Modelled: 527 Confidence: 100.0% Identity: 25% PDB header:lyase Chain: D: PDB Molecule:glutaconyl-coa decarboxylase subunit a; PDBTitle: glutaconyl-coa decarboxylase a subunit from clostridium symbiosum co-2 crystallized with crotonyl-coa Phyre2 PDB 1on3 chain E Region: 14 - 527 Aligned: 500 Modelled: 514 Confidence: 100.0% Identity: 35% PDB header:transferase Chain: E: PDB Molecule:methylmalonyl-coa carboxyltransferase 12s PDBTitle: transcarboxylase 12s crystal structure: hexamer assembly2 and substrate binding to a multienzyme core (with3 methylmalonyl-coenzyme a and methylmalonic acid bound) Phyre2 PDB 1vrg chain E Region: 16 - 522 Aligned: 493 Modelled: 507 Confidence: 100.0% Identity: 34% PDB header:ligase Chain: E: PDB Molecule:propionyl-coa carboxylase, beta subunit; PDBTitle: crystal structure of propionyl-coa carboxylase, beta subunit (tm0716)2 from thermotoga maritima at 2.30 a resolution Phyre2 PDB 1xnw chain D Region: 14 - 525 Aligned: 500 Modelled: 512 Confidence: 100.0% Identity: 33% PDB header:ligase Chain: D: PDB Molecule:propionyl-coa carboxylase complex b subunit; PDBTitle: acyl-coa carboxylase beta subunit from s. coelicolor (pccb),2 apo form #2, mutant d422i Phyre2 PDB 2a7s chain D Region: 14 - 522 Aligned: 499 Modelled: 508 Confidence: 100.0% Identity: 33% PDB header:ligase Chain: D: PDB Molecule:probable propionyl-coa carboxylase beta chain 5; PDBTitle: crystal structure of the acyl-coa carboxylase, accd5, from2 mycobacterium tuberculosis Phyre2 PDB 1x0u chain B Region: 16 - 525 Aligned: 497 Modelled: 510 Confidence: 100.0% Identity: 32% PDB header:lyase Chain: B: PDB Molecule:hypothetical methylmalonyl-coa decarboxylase alpha subunit; PDBTitle: crystal structure of the carboxyl transferase subunit of putative pcc2 of sulfolobus tokodaii Phyre2 PDB 5fif chain D Region: 3 - 523 Aligned: 502 Modelled: 520 Confidence: 100.0% Identity: 30% PDB header:ligase Chain: D: PDB Molecule:carboxylase; PDBTitle: carboxyltransferase domain of a single-chain bacterial carboxylase Phyre2 PDB 4rcn chain A Region: 8 - 509 Aligned: 472 Modelled: 502 Confidence: 100.0% Identity: 28% PDB header:ligase Chain: A: PDB Molecule:long-chain acyl-coa carboxylase; PDBTitle: structure and function of a single-chain, multi-domain long-chain2 acyl-coa carboxylase Phyre2 PDB 3n6r chain F Region: 20 - 522 Aligned: 486 Modelled: 502 Confidence: 100.0% Identity: 35% PDB header:ligase Chain: F: PDB Molecule:propionyl-coa carboxylase, beta subunit; PDBTitle: crystal structure of the holoenzyme of propionyl-coa carboxylase (pcc) Phyre2 PDB 5ing chain C Region: 16 - 522 Aligned: 480 Modelled: 485 Confidence: 100.0% Identity: 30% PDB header:transferase Chain: C: PDB Molecule:putative carboxyl transferase; PDBTitle: a crotonyl-coa reductase-carboxylase independent pathway for assembly2 of unusual alkylmalonyl-coa polyketide synthase extender unit Phyre2 PDB 4l6w chain A Region: 49 - 514 Aligned: 402 Modelled: 424 Confidence: 100.0% Identity: 32% PDB header:ligase Chain: A: PDB Molecule:probable propionyl-coa carboxylase beta chain 6; PDBTitle: carboxyltransferase subunit (accd6) of mycobacterium tuberculosis2 acetyl-coa carboxylase Phyre2 PDB 4l6w chain B Region: 49 - 518 Aligned: 410 Modelled: 432 Confidence: 100.0% Identity: 31% PDB header:ligase Chain: B: PDB Molecule:probable propionyl-coa carboxylase beta chain 6; PDBTitle: carboxyltransferase subunit (accd6) of mycobacterium tuberculosis2 acetyl-coa carboxylase Phyre2 PDB 1uyv chain B Region: 1 - 517 Aligned: 479 Modelled: 517 Confidence: 100.0% Identity: 23% PDB header:transferase Chain: B: PDB Molecule:acetyl-coa carboxylase; PDBTitle: acetyl-coa carboxylase carboxyltransferase domain l1705i/2 v1967i mutant Phyre2 PDB 4wyo chain B Region: 1 - 521 Aligned: 501 Modelled: 521 Confidence: 100.0% Identity: 23% PDB header:ligase/ligase inhibitor Chain: B: PDB Molecule:acetyl-coa carboxylase; PDBTitle: crystal structure of human-yeast chimera acetyl coa carboxylase ct2 domain bound to compound 1 Phyre2 PDB 3h0j chain A Region: 1 - 521 Aligned: 496 Modelled: 521 Confidence: 100.0% Identity: 23% PDB header:transferase Chain: A: PDB Molecule:acetyl-coa carboxylase; PDBTitle: crystal structure of the carboxyltransferase domain of2 acetyl-coenzyme a carboxylase in complex with compound 2 Phyre2 PDB 2x24 chain B Region: 36 - 521 Aligned: 471 Modelled: 486 Confidence: 100.0% Identity: 24% PDB header:ligase Chain: B: PDB Molecule:acetyl-coa carboxylase; PDBTitle: bovine acc2 ct domain in complex with inhibitor Phyre2 1 c4q0gC_ 2 c3u9rB_ 3 c5iklD_ 4 c1pixB_ 5 c3glmD_ 6 c1on3E_ 7 c1vrgE_ 8 c1xnwD_ 9 c2a7sD_ 10 c1x0uB_ 11 c5fifD_ 12 c4rcnA_ 13 c3n6rF_ 14 c5ingC_ 15 c4l6wA_ 16 c4l6wB_ 17 c1uyvB_ 18 c4wyoB_ 19 c3h0jA_ 20 c2x24B_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251