1

.

.

.

.

.

.

.

.

10

.

.

.

.

.

.

.

.

.

20

.

.

.

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.

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.

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30

.

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40

.

.

.

.

.

.

.

.

.

50

.

.

.

.

.

.

.

.

.

60

Sequence

M

R

R

R

R

P

P

H

V

N

A

P

T

P

C

D

R

G

D

V

R

P

P

G

C

P

A

S

I

P

G

V

E

V

A

G

G

T

R

A

R

L

R

V

T

A

D

G

L

Q

A

L

A

G

R

C

A

T

L

A

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

.

.

.

.

.

.

.

70

.

.

.

.

.

.

.

.

.

80

.

.

.

.

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.

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.

.

90

.

.

.

.

.

.

.

.

.

100

.

.

.

.

.

.

.

.

.

110

.

.

.

.

.

.

.

.

.

120

Sequence

G

E

L

S

A

A

V

A

P

S

G

A

V

L

S

W

Q

A

N

A

V

A

V

N

A

A

H

A

R

A

G

A

A

A

A

A

V

S

A

R

M

R

A

T

A

A

A

L

G

Q

A

A

R

R

Y

A

G

Q

D

T

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

.

.

.

.

.

.

.

130

.

.

.

.

.

Sequence

A

A

A

A

A

L

G

A

V

R

P

W

G

T

H

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 59%) Alpha helix ( 53%) Beta strand ( 7%)

Rank Aligned region

PDB 4xy3 chain A Region: 49 - 115 Aligned: 67 Modelled: 67 Confidence: 31.4% Identity: 18% PDB header:protein transport Chain: A: PDB Molecule:esx-1 secretion-associated protein espb; PDBTitle: structure of esx-1 secreted protein espb Phyre2 PDB 1wa8 chain A domain 1 Region: 40 - 63 Aligned: 24 Modelled: 24 Confidence: 31.0% Identity: 29% Fold: Ferritin-like Superfamily: EsxAB dimer-like Family: ESAT-6 like Phyre2 PDB 4iog chain D Region: 40 - 63 Aligned: 24 Modelled: 24 Confidence: 28.6% Identity: 33% PDB header:unknown function Chain: D: PDB Molecule:secreted protein esxb; PDBTitle: the crystal structure of a secreted protein esxb (wild-type, in p212 space group) from bacillus anthracis str. sterne Phyre2 PDB 5xfs chain B Region: 47 - 115 Aligned: 69 Modelled: 69 Confidence: 13.1% Identity: 22% PDB header:protein transport Chain: B: PDB Molecule:ppe family protein ppe15; PDBTitle: crystal structure of pe8-ppe15 in complex with espg5 from m.2 tuberculosis Phyre2 PDB 3gvm chain A Region: 37 - 63 Aligned: 27 Modelled: 27 Confidence: 12.5% Identity: 15% PDB header:viral protein Chain: A: PDB Molecule:putative uncharacterized protein sag1039; PDBTitle: structure of the homodimeric wxg-100 family protein from streptococcus2 agalactiae Phyre2 PDB 2vs0 chain B Region: 43 - 63 Aligned: 21 Modelled: 19 Confidence: 12.1% Identity: 5% PDB header:cell invasion Chain: B: PDB Molecule:virulence factor esxa; PDBTitle: structural analysis of homodimeric staphylococcal aureus2 virulence factor esxa Phyre2 PDB 3dzm chain B Region: 49 - 76 Aligned: 28 Modelled: 28 Confidence: 11.6% Identity: 21% PDB header:unknown function Chain: B: PDB Molecule:hypothetical conserved protein; PDBTitle: crystal structure of a major outer membrane protein from thermus2 thermophilus hb27 Phyre2 PDB 1khb chain A domain 2 Region: 22 - 34 Aligned: 13 Modelled: 13 Confidence: 6.7% Identity: 46% Fold: PEP carboxykinase N-terminal domain Superfamily: PEP carboxykinase N-terminal domain Family: PEP carboxykinase N-terminal domain Phyre2 PDB 1ic9 chain A Region: 21 - 30 Aligned: 10 Modelled: 10 Confidence: 6.1% Identity: 60% PDB header:de novo protein Chain: A: PDB Molecule:th10aox; PDBTitle: nmr solution structure of the designed beta-sheet mini-2 protein th10aox Phyre2 PDB 5cmo chain B Region: 47 - 77 Aligned: 31 Modelled: 31 Confidence: 5.8% Identity: 19% PDB header:transferase Chain: B: PDB Molecule:holo-[acyl-carrier-protein] synthase; PDBTitle: crystal structure of holo-[acyl-carrier-protein] synthase (acps) from2 neisseria meningitidis Phyre2 PDB 1icl chain A Region: 21 - 30 Aligned: 10 Modelled: 10 Confidence: 5.6% Identity: 60% PDB header:de novo protein Chain: A: PDB Molecule:th1ox; PDBTitle: solution structure of designed beta-sheet mini-protein th1ox Phyre2 1 c4xy3A_ 2 d1wa8a1 3 c4iogD_ 4 c5xfsB_ 5 c3gvmA_ 6 c2vs0B_ 7 c3dzmB_ 8 d1khba2 9 c1ic9A_ 10 c5cmoB_ 11 c1iclA_