1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	A	S	L	L	V	R	T	A	C	G	G	R	A	V	A	Q	R	L	R	T	V	L	W	P	I	T	Q	T	S	V	V	A	G	L	A	W	Y	L	T	H	D	V	F	N	H	P	Q	A	F	F	A	P	I	S	A	V	V	C
Secondary structure
SS confidence
Disorder		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		M	S	A	T	N	V	L	R	A	R	R	A	Q	Q	M	I	V	G	V	A	L	G	I	V	L	G	A	G	V	H	A	L	L	G	S	G	P	I	A	M	G	V	V	V	F	I	A	L	S	V	A	V	L	C	A	R	G	L	V	A
Secondary structure
SS confidence
Disorder																																																										?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		Q	G	L	M	F	I	N	Q	A	A	V	S	A	V	L	V	L	V	F	A	S	N	G	S	V	V	F	E	R	L	F	D	A	L	V	G	G	G	L	A	I	V	F	S	I	L	L	F	P	P	D	P	V	V	M	L	C	S	A	R
Secondary structure
SS confidence
Disorder																				?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		A	D	V	L	A	A	V	R	D	I	L	A	E	L	V	N	T	V	S	D	P	T	S	A	P	P	D	W	P	M	A	A	A	D	R	L	H	Q	Q	L	N	G	L	I	E	V	R	A	N	A	A	M	V	A	R	R	A	P	R	R
Secondary structure
SS confidence
Disorder																					?	?	?	?	?	?																																			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		W	G	V	R	S	T	V	R	D	L	D	Q	Q	A	V	Y	L	A	L	L	V	S	S	V	L	H	L	A	R	T	I	A	G	P	G	G	D	K	L	P	T	P	V	H	A	V	L	T	D	L	A	A	G	T	G	L	A	D	A	D
Secondary structure
SS confidence
Disorder		?	?		?	?																										?	?	?	?	?	?	?	?	?																					?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.
Sequence		P	T	A	A	N	E	H	A	A	A	A	R	A	T	A	S	T	L	Q	S	A	A	C	G	S	N	E	V	V	R	A	D	I	V	Q	A	C	V	T	D	L	Q	R	V	I	E	R	P	G	P	S	G	M	S	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?										?	?	?	?	?	?	?	?	?																							?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 15%) Alpha helix ( 80%) Beta strand ( 0%) TM helix ( 31%) (View membrane topology prediction)

Rank Aligned region

PDB 5nva chain A Region: 62 - 172 Aligned: 111 Modelled: 111 Confidence: 60.3% Identity: 14% PDB header:membrane protein Chain: A: PDB Molecule:putative sodium:solute symporter; PDBTitle: substrate-bound outward-open state of a na+-coupled sialic acid2 symporter reveals a novel na+-site Phyre2 PDB 6drh chain C Region: 70 - 93 Aligned: 24 Modelled: 24 Confidence: 37.8% Identity: 17% PDB header:toxin Chain: C: PDB Molecule:adp-ribosyl-(dinitrogen reductase) hydrolase; PDBTitle: adp-ribosyltransferase toxin/immunity pair Phyre2 PDB 5khn chain B Region: 51 - 169 Aligned: 118 Modelled: 119 Confidence: 30.3% Identity: 13% PDB header:membrane protein Chain: B: PDB Molecule:rnd transporter; PDBTitle: crystal structures of the burkholderia multivorans hopanoid2 transporter hpnn Phyre2 PDB 3hfw chain A Region: 73 - 91 Aligned: 19 Modelled: 19 Confidence: 29.3% Identity: 11% PDB header:hydrolase Chain: A: PDB Molecule:protein adp-ribosylarginine hydrolase; PDBTitle: crystal structure of human adp-ribosylhydrolase 1 (harh1) Phyre2 PDB 1ots chain A Region: 76 - 186 Aligned: 97 Modelled: 111 Confidence: 28.3% Identity: 13% Fold: Clc chloride channel Superfamily: Clc chloride channel Family: Clc chloride channel Phyre2 PDB 2ht2 chain B Region: 76 - 180 Aligned: 91 Modelled: 105 Confidence: 26.4% Identity: 13% PDB header:membrane protein Chain: B: PDB Molecule:h(+)/cl(-) exchange transporter clca; PDBTitle: structure of the escherichia coli clc chloride channel2 y445h mutant and fab complex Phyre2 PDB 6ith chain A Region: 74 - 93 Aligned: 20 Modelled: 20 Confidence: 22.1% Identity: 25% PDB header:membrane protein Chain: A: PDB Molecule:syndecan-2; PDBTitle: structure of the transmembrane domain of syndecan 2 in micelles Phyre2 PDB 6ajj chain A Region: 55 - 169 Aligned: 115 Modelled: 115 Confidence: 20.1% Identity: 17% PDB header:membrane protein, hydrolase Chain: A: PDB Molecule:drug exporters of the rnd superfamily-like protein, PDBTitle: crystal structure of mycolic acid transporter mmpl3 from mycobacterium2 smegmatis complexed with ica38 Phyre2 PDB 2l35 chain B Region: 78 - 93 Aligned: 16 Modelled: 16 Confidence: 19.8% Identity: 50% PDB header:protein binding Chain: B: PDB Molecule:tyro protein tyrosine kinase-binding protein; PDBTitle: structure of the dap12-nkg2c transmembrane heterotrimer Phyre2 PDB 2l34 chain A Region: 78 - 93 Aligned: 16 Modelled: 16 Confidence: 19.4% Identity: 50% PDB header:protein binding Chain: A: PDB Molecule:tyro protein tyrosine kinase-binding protein; PDBTitle: structure of the dap12 transmembrane homodimer Phyre2 PDB 4wol chain A Region: 78 - 93 Aligned: 16 Modelled: 16 Confidence: 19.4% Identity: 50% PDB header:signaling protein Chain: A: PDB Molecule:tyro protein tyrosine kinase-binding protein; PDBTitle: crystal structure of the dap12 transmembrane domain in lipidic cubic2 phase Phyre2 PDB 2l34 chain B Region: 78 - 93 Aligned: 16 Modelled: 16 Confidence: 19.4% Identity: 50% PDB header:protein binding Chain: B: PDB Molecule:tyro protein tyrosine kinase-binding protein; PDBTitle: structure of the dap12 transmembrane homodimer Phyre2 PDB 4wo1 chain D Region: 78 - 93 Aligned: 16 Modelled: 16 Confidence: 18.4% Identity: 50% PDB header:signaling protein Chain: D: PDB Molecule:tyro protein tyrosine kinase-binding protein; PDBTitle: crystal structure of the dap12 transmembrane domain in lipid cubic2 phase Phyre2 PDB 4wo1 chain A Region: 78 - 93 Aligned: 16 Modelled: 16 Confidence: 18.4% Identity: 50% PDB header:signaling protein Chain: A: PDB Molecule:tyro protein tyrosine kinase-binding protein; PDBTitle: crystal structure of the dap12 transmembrane domain in lipid cubic2 phase Phyre2 PDB 4wo1 chain B Region: 78 - 93 Aligned: 16 Modelled: 16 Confidence: 18.4% Identity: 50% PDB header:signaling protein Chain: B: PDB Molecule:tyro protein tyrosine kinase-binding protein; PDBTitle: crystal structure of the dap12 transmembrane domain in lipid cubic2 phase Phyre2 PDB 4wo1 chain C Region: 78 - 93 Aligned: 16 Modelled: 16 Confidence: 18.4% Identity: 50% PDB header:signaling protein Chain: C: PDB Molecule:tyro protein tyrosine kinase-binding protein; PDBTitle: crystal structure of the dap12 transmembrane domain in lipid cubic2 phase Phyre2 PDB 6csx chain A Region: 51 - 169 Aligned: 119 Modelled: 119 Confidence: 18.4% Identity: 16% PDB header:transport protein Chain: A: PDB Molecule:multidrug efflux pump subunit acrb; PDBTitle: single particles cryo-em structure of acrb d407a associated with lipid2 bilayer at 3.0 angstrom Phyre2 PDB 6g1q chain A Region: 73 - 90 Aligned: 18 Modelled: 18 Confidence: 18.3% Identity: 22% PDB header:hydrolase Chain: A: PDB Molecule:adp-ribosylhydrolase like 2; PDBTitle: adp-ribosylserine hydrolase arh3 of latimeria chalumnae in complex2 with adp-ribose Phyre2 PDB 4wol chain C Region: 78 - 93 Aligned: 16 Modelled: 16 Confidence: 18.0% Identity: 50% PDB header:signaling protein Chain: C: PDB Molecule:tyro protein tyrosine kinase-binding protein; PDBTitle: crystal structure of the dap12 transmembrane domain in lipidic cubic2 phase Phyre2 PDB 4wol chain B Region: 78 - 93 Aligned: 16 Modelled: 16 Confidence: 18.0% Identity: 50% PDB header:signaling protein Chain: B: PDB Molecule:tyro protein tyrosine kinase-binding protein; PDBTitle: crystal structure of the dap12 transmembrane domain in lipidic cubic2 phase Phyre2 1 c5nvaA_ 2 c6drhC_ 3 c5khnB_ 4 c3hfwA_ 5 d1otsa_ 6 c2ht2B_ 7 c6ithA_ 8 c6ajjA_ 9 c2l35B_ 10 c2l34A_ 11 c4wolA_ 12 c2l34B_ 13 c4wo1D_ 14 c4wo1A_ 15 c4wo1B_ 16 c4wo1C_ 17 c6csxA_ 18 c6g1qA_ 19 c4wolC_ 20 c4wolB_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99