1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	A	S	K	A	K	T	G	R	D	D	E	A	T	S	A	V	E	L	T	E	A	T	E	S	A	V	A	R	T	D	G	D	S	T	T	D	T	A	S	K	L	G	H	H	S	F	L	S	R	L	Y	T	G	T	G	A	F	E	V
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?														?	?	?		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		V	G	R	R	R	L	W	F	G	V	S	G	A	I	V	A	V	A	I	A	S	I	V	F	R	G	F	T	F	G	I	D	F	K	G	G	T	T	V	S	F	P	R	G	S	T	Q	V	A	Q	V	E	D	V	Y	Y	R	A	L	G
Secondary structure
SS confidence
Disorder																																															?	?											?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		S	E	P	Q	S	V	V	I	V	G	A	G	A	S	A	T	V	Q	I	R	S	E	T	L	T	S	D	Q	T	A	K	L	R	D	A	L	F	E	A	F	G	P	K	G	T	D	G	Q	P	S	K	Q	A	I	S	D	S	A	V	S
Secondary structure
SS confidence
Disorder		?	?	?	?	?				?	?	?	?	?	?	?						?	?	?	?	?															?	?	?	?	?	?	?	?	?	?	?	?	?		?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		E	T	W	G	G	Q	I	T	K	K	A	V	I	A	L	V	V	F	L	V	L	V	A	L	Y	I	T	V	R	Y	E	R	Y	M	T	I	S	A	I	T	A	M	L	F	D	L	T	V	T	A	G	V	Y	S	L	V	G	F	E	V
Secondary structure
SS confidence
Disorder																																	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		T	P	A	T	V	I	G	L	L	T	I	L	G	F	S	L	Y	D	T	V	I	V	F	D	K	V	E	E	N	T	H	G	F	Q	H	T	T	R	R	T	F	A	E	Q	A	N	L	A	I	N	Q	T	F	M	R	S	I	N	T	S
Secondary structure
SS confidence
Disorder																																	?	?		?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		L	I	G	V	L	P	V	L	A	L	M	V	V	A	V	W	L	L	G	V	G	T	L	K	D	L	A	L	V	Q	L	I	G	I	I	I	G	T	Y	S	S	I	F	F	A	T	P	L	L	V	T	L	R	E	R	T	E	L	V	R
Secondary structure
SS confidence
Disorder																			?		?	?	?	?																															?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		N	H	T	R	R	V	L	K	R	R	N	S	G	S	P	A	G	S	E	D	A	S	T	D	G	G	E	Q	P	A	A	A	D	E	Q	S	L	V	G	I	T	Q	A	S	S	Q	S	A	P	R	A	A	Q	G	S	S	K	P	A	P
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.
Sequence		G	A	R	P	V	R	P	V	G	T	R	R	P	T	G	K	R	N	A	G	R	R
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 40%) Alpha helix ( 55%) Beta strand ( 6%) TM helix ( 31%) (View membrane topology prediction)

Rank Aligned region

PDB 5mg3 chain F Region: 55 - 355 Aligned: 289 Modelled: 301 Confidence: 100.0% Identity: 25% PDB header:chaperone Chain: F: PDB Molecule:protein translocase subunit secf; PDBTitle: em fitted model of bacterial holo-translocon Phyre2 PDB 3aqp chain B Region: 50 - 355 Aligned: 287 Modelled: 306 Confidence: 100.0% Identity: 29% PDB header:membrane protein Chain: B: PDB Molecule:probable secdf protein-export membrane protein; PDBTitle: crystal structure of secdf, a translocon-associated membrane protein,2 from thermus thrmophilus Phyre2 PDB 5xam chain A Region: 42 - 358 Aligned: 300 Modelled: 314 Confidence: 100.0% Identity: 27% PDB header:membrane protein Chain: A: PDB Molecule:protein translocase subunit secd; PDBTitle: crystal structure of secdf in i form at 4 a resolution Phyre2 PDB 5mg3 chain D Region: 122 - 360 Aligned: 221 Modelled: 234 Confidence: 99.9% Identity: 15% PDB header:chaperone Chain: D: PDB Molecule:protein translocase subunit secd; PDBTitle: em fitted model of bacterial holo-translocon Phyre2 PDB 5lq3 chain F Region: 132 - 365 Aligned: 225 Modelled: 234 Confidence: 99.9% Identity: 15% PDB header:transport protein Chain: F: PDB Molecule:cmeb; PDBTitle: structures and transport dynamics of the campylobacter jejuni2 multidrug efflux pump cmeb Phyre2 PDB 2v50 chain A Region: 132 - 369 Aligned: 228 Modelled: 238 Confidence: 99.9% Identity: 14% PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein mexb; PDBTitle: the missing part of the bacterial mexab-oprm system: structural2 determination of the multidrug exporter mexb Phyre2 PDB 6ows chain B Region: 132 - 365 Aligned: 224 Modelled: 234 Confidence: 99.9% Identity: 14% PDB header:membrane protein Chain: B: PDB Molecule:efflux pump membrane transporter; PDBTitle: cryo-em structure of an acinetobacter baumannii multidrug efflux pump Phyre2 PDB 6ajj chain A Region: 63 - 365 Aligned: 285 Modelled: 300 Confidence: 99.8% Identity: 16% PDB header:membrane protein, hydrolase Chain: A: PDB Molecule:drug exporters of the rnd superfamily-like protein, PDBTitle: crystal structure of mycolic acid transporter mmpl3 from mycobacterium2 smegmatis complexed with ica38 Phyre2 PDB 4mt1 chain A Region: 132 - 363 Aligned: 222 Modelled: 227 Confidence: 99.8% Identity: 15% PDB header:membrane protein, tranport protein Chain: A: PDB Molecule:drug efflux protein; PDBTitle: crystal structure of the neisseria gonorrhoeae mtrd inner membrane2 multidrug efflux pump Phyre2 PDB 3k07 chain A Region: 122 - 371 Aligned: 247 Modelled: 250 Confidence: 99.8% Identity: 11% PDB header:transport protein Chain: A: PDB Molecule:cation efflux system protein cusa; PDBTitle: crystal structure of cusa Phyre2 PDB 4k0e chain A Region: 76 - 355 Aligned: 259 Modelled: 279 Confidence: 99.8% Identity: 12% PDB header:transport protein Chain: A: PDB Molecule:heavy metal cation tricomponent efflux pump znea(czca- PDBTitle: x-ray crystal structure of a heavy metal efflux pump, crystal form ii Phyre2 PDB 6csx chain A Region: 132 - 362 Aligned: 221 Modelled: 231 Confidence: 99.8% Identity: 15% PDB header:transport protein Chain: A: PDB Molecule:multidrug efflux pump subunit acrb; PDBTitle: single particles cryo-em structure of acrb d407a associated with lipid2 bilayer at 3.0 angstrom Phyre2 PDB 1oy8 chain A Region: 132 - 364 Aligned: 223 Modelled: 228 Confidence: 99.8% Identity: 15% PDB header:membrane protein Chain: A: PDB Molecule:acriflavine resistance protein b; PDBTitle: structural basis of multiple drug binding capacity of the acrb2 multidrug efflux pump Phyre2 PDB 5khn chain B Region: 59 - 371 Aligned: 297 Modelled: 308 Confidence: 99.8% Identity: 17% PDB header:membrane protein Chain: B: PDB Molecule:rnd transporter; PDBTitle: crystal structures of the burkholderia multivorans hopanoid2 transporter hpnn Phyre2 PDB 1iwg chain A domain 7 Region: 147 - 354 Aligned: 190 Modelled: 199 Confidence: 99.8% Identity: 16% Fold: Multidrug efflux transporter AcrB transmembrane domain Superfamily: Multidrug efflux transporter AcrB transmembrane domain Family: Multidrug efflux transporter AcrB transmembrane domain Phyre2 PDB 1iwg chain A domain 8 Region: 189 - 358 Aligned: 164 Modelled: 170 Confidence: 99.8% Identity: 20% Fold: Multidrug efflux transporter AcrB transmembrane domain Superfamily: Multidrug efflux transporter AcrB transmembrane domain Family: Multidrug efflux transporter AcrB transmembrane domain Phyre2 PDB 6dmo chain A Region: 141 - 364 Aligned: 215 Modelled: 219 Confidence: 99.7% Identity: 16% PDB header:protein binding Chain: A: PDB Molecule:protein patched homolog 1; PDBTitle: cryo-em structure of human ptch1 with three mutations2 l282q/t500f/p504l Phyre2 PDB 4k0e chain C Region: 185 - 352 Aligned: 151 Modelled: 168 Confidence: 99.7% Identity: 17% PDB header:transport protein Chain: C: PDB Molecule:heavy metal cation tricomponent efflux pump znea(czca- PDBTitle: x-ray crystal structure of a heavy metal efflux pump, crystal form ii Phyre2 PDB 3jd8 chain A Region: 141 - 352 Aligned: 201 Modelled: 212 Confidence: 99.6% Identity: 13% PDB header:membrane protein Chain: A: PDB Molecule:niemann-pick c1 protein; PDBTitle: cryo-em structure of the full-length human npc1 at 4.4 angstrom Phyre2 PDB 2rrn chain A Region: 84 - 180 Aligned: 88 Modelled: 97 Confidence: 99.5% Identity: 18% PDB header:protein transport Chain: A: PDB Molecule:probable secdf protein-export membrane protein; PDBTitle: solution structure of secdf periplasmic domain p4 Phyre2 1 c5mg3F_ 2 c3aqpB_ 3 c5xamA_ 4 c5mg3D_ 5 c5lq3F_ 6 c2v50A_ 7 c6owsB_ 8 c6ajjA_ 9 c4mt1A_ 10 c3k07A_ 11 c4k0eA_ 12 c6csxA_ 13 c1oy8A_ 14 c5khnB_ 15 d1iwga7 16 d1iwga8 17 c6dmoA_ 18 c4k0eC_ 19 c3jd8A_ 20 c2rrnA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99