1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	A	S	S	S	A	P	V	H	P	A	R	Y	L	S	V	F	L	V	M	L	I	G	I	Y	L	L	V	F	F	T	G	D	K	H	T	A	P	K	L	G	I	D	L	Q	G	G	T	R	V	T	L	T	A	R	T	P	D	G	S
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?																					?			?	?	?																					?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		A	P	S	R	E	A	L	A	Q	A	Q	Q	I	I	S	A	R	V	N	G	L	G	V	S	G	S	E	V	V	V	D	G	D	N	L	V	I	T	V	P	G	N	D	G	S	E	A	R	N	L	G	Q	T	A	R	L	Y	I	R	P
Secondary structure
SS confidence
Disorder		?	?	?																		?	?		?	?		?	?		?	?	?	?									?	?																	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		V	L	N	S	M	P	A	Q	P	A	A	E	E	P	Q	P	A	P	S	A	E	P	Q	P	P	G	Q	P	A	A	P	P	P	A	Q	S	G	A	P	A	S	P	Q	P	G	A	Q	P	R	P	Y	P	Q	D	P	A	P	S	P	N
Secondary structure
SS confidence
Disorder				?		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		P	T	S	P	A	S	P	P	P	A	P	P	A	E	A	P	A	T	D	P	R	K	D	L	A	E	R	I	A	Q	E	K	K	L	R	Q	S	T	N	Q	Y	M	Q	M	V	A	L	Q	F	Q	A	T	R	C	E	S	D	D	I	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?		?	?	?	?	?														?	?	?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		A	G	N	D	D	P	K	L	P	L	V	T	C	S	T	D	H	K	T	A	Y	L	L	A	P	S	I	I	S	G	D	Q	I	Q	N	A	T	S	G	M	D	Q	R	G	I	G	Y	V	V	D	L	Q	F	K	G	P	A	A	N	I
Secondary structure
SS confidence
Disorder		?	?			?	?	?																				?	?	?	?				?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		W	A	D	Y	T	A	A	H	I	G	T	Q	T	A	F	T	L	D	S	Q	V	V	S	A	P	Q	I	Q	E	A	I	P	G	G	R	T	Q	I	S	G	G	D	P	P	F	T	A	A	T	A	R	Q	L	A	N	V	L	K	Y	G
Secondary structure
SS confidence
Disorder																												?	?	?	?	?	?	?	?	?					?	?	?	?	?	?	?											?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		S	L	P	L	S	F	E	P	S	E	A	Q	T	V	S	A	T	L	G	L	S	S	L	R	A	G	M	I	A	G	A	I	G	L	L	L	V	L	V	Y	S	L	L	Y	Y	R	V	L	G	L	L	T	A	L	S	L	V	A	S	G
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		S	M	V	F	A	I	L	V	L	L	G	R	Y	I	N	Y	T	L	D	L	A	G	I	A	G	L	I	I	G	I	G	T	T	A	D	S	F	V	V	F	F	E	R	I	K	D	E	I	R	E	G	R	S	F	R	S	A	V	P	R
Secondary structure
SS confidence
Disorder																?																																			?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		G	W	A	R	A	R	K	T	I	V	S	G	N	A	V	T	F	L	A	A	A	V	L	Y	F	L	A	I	G	Q	V	K	G	F	A	F	T	L	G	L	T	T	I	L	D	L	V	V	V	F	L	V	T	W	P	L	V	Y	L	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560	.	.	.	.	.	.	.	.	.	570	.	.	.
Sequence		S	K	S	S	L	L	A	K	P	A	Y	N	G	L	G	A	V	Q	Q	V	A	R	E	R	R	A	M	A	R	T	G	R	G
Secondary structure
SS confidence
Disorder			?	?	?	?	?	?	?		?		?			?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 39%) Alpha helix ( 50%) Beta strand ( 10%) TM helix ( 22%) (View membrane topology prediction)

Rank Aligned region

PDB 3aqp chain B Region: 9 - 564 Aligned: 444 Modelled: 455 Confidence: 100.0% Identity: 28% PDB header:membrane protein Chain: B: PDB Molecule:probable secdf protein-export membrane protein; PDBTitle: crystal structure of secdf, a translocon-associated membrane protein,2 from thermus thrmophilus Phyre2 PDB 5xam chain A Region: 9 - 565 Aligned: 431 Modelled: 440 Confidence: 100.0% Identity: 30% PDB header:membrane protein Chain: A: PDB Molecule:protein translocase subunit secd; PDBTitle: crystal structure of secdf in i form at 4 a resolution Phyre2 PDB 5lq3 chain F Region: 8 - 551 Aligned: 489 Modelled: 501 Confidence: 100.0% Identity: 13% PDB header:transport protein Chain: F: PDB Molecule:cmeb; PDBTitle: structures and transport dynamics of the campylobacter jejuni2 multidrug efflux pump cmeb Phyre2 PDB 2v50 chain A Region: 7 - 545 Aligned: 483 Modelled: 491 Confidence: 100.0% Identity: 14% PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein mexb; PDBTitle: the missing part of the bacterial mexab-oprm system: structural2 determination of the multidrug exporter mexb Phyre2 PDB 6ows chain B Region: 3 - 551 Aligned: 493 Modelled: 505 Confidence: 100.0% Identity: 13% PDB header:membrane protein Chain: B: PDB Molecule:efflux pump membrane transporter; PDBTitle: cryo-em structure of an acinetobacter baumannii multidrug efflux pump Phyre2 PDB 3k07 chain A Region: 8 - 548 Aligned: 485 Modelled: 498 Confidence: 100.0% Identity: 10% PDB header:transport protein Chain: A: PDB Molecule:cation efflux system protein cusa; PDBTitle: crystal structure of cusa Phyre2 PDB 4mt1 chain A Region: 4 - 548 Aligned: 489 Modelled: 509 Confidence: 100.0% Identity: 13% PDB header:membrane protein, tranport protein Chain: A: PDB Molecule:drug efflux protein; PDBTitle: crystal structure of the neisseria gonorrhoeae mtrd inner membrane2 multidrug efflux pump Phyre2 PDB 1oy8 chain A Region: 10 - 548 Aligned: 483 Modelled: 503 Confidence: 100.0% Identity: 15% PDB header:membrane protein Chain: A: PDB Molecule:acriflavine resistance protein b; PDBTitle: structural basis of multiple drug binding capacity of the acrb2 multidrug efflux pump Phyre2 PDB 6csx chain A Region: 8 - 546 Aligned: 483 Modelled: 503 Confidence: 100.0% Identity: 16% PDB header:transport protein Chain: A: PDB Molecule:multidrug efflux pump subunit acrb; PDBTitle: single particles cryo-em structure of acrb d407a associated with lipid2 bilayer at 3.0 angstrom Phyre2 PDB 4k0e chain A Region: 8 - 548 Aligned: 468 Modelled: 489 Confidence: 100.0% Identity: 14% PDB header:transport protein Chain: A: PDB Molecule:heavy metal cation tricomponent efflux pump znea(czca- PDBTitle: x-ray crystal structure of a heavy metal efflux pump, crystal form ii Phyre2 PDB 4k0e chain C Region: 8 - 551 Aligned: 463 Modelled: 484 Confidence: 100.0% Identity: 15% PDB header:transport protein Chain: C: PDB Molecule:heavy metal cation tricomponent efflux pump znea(czca- PDBTitle: x-ray crystal structure of a heavy metal efflux pump, crystal form ii Phyre2 PDB 5mg3 chain D Region: 159 - 548 Aligned: 370 Modelled: 378 Confidence: 100.0% Identity: 24% PDB header:chaperone Chain: D: PDB Molecule:protein translocase subunit secd; PDBTitle: em fitted model of bacterial holo-translocon Phyre2 PDB 5mg3 chain F Region: 356 - 543 Aligned: 184 Modelled: 188 Confidence: 100.0% Identity: 18% PDB header:chaperone Chain: F: PDB Molecule:protein translocase subunit secf; PDBTitle: em fitted model of bacterial holo-translocon Phyre2 PDB 6ajj chain A Region: 337 - 563 Aligned: 225 Modelled: 227 Confidence: 100.0% Identity: 15% PDB header:membrane protein, hydrolase Chain: A: PDB Molecule:drug exporters of the rnd superfamily-like protein, PDBTitle: crystal structure of mycolic acid transporter mmpl3 from mycobacterium2 smegmatis complexed with ica38 Phyre2 PDB 6dmo chain A Region: 9 - 549 Aligned: 474 Modelled: 479 Confidence: 100.0% Identity: 11% PDB header:protein binding Chain: A: PDB Molecule:protein patched homolog 1; PDBTitle: cryo-em structure of human ptch1 with three mutations2 l282q/t500f/p504l Phyre2 PDB 5khn chain B Region: 9 - 544 Aligned: 409 Modelled: 436 Confidence: 100.0% Identity: 15% PDB header:membrane protein Chain: B: PDB Molecule:rnd transporter; PDBTitle: crystal structures of the burkholderia multivorans hopanoid2 transporter hpnn Phyre2 PDB 1iwg chain A domain 8 Region: 337 - 544 Aligned: 204 Modelled: 208 Confidence: 100.0% Identity: 12% Fold: Multidrug efflux transporter AcrB transmembrane domain Superfamily: Multidrug efflux transporter AcrB transmembrane domain Family: Multidrug efflux transporter AcrB transmembrane domain Phyre2 PDB 1iwg chain A domain 7 Region: 343 - 542 Aligned: 195 Modelled: 200 Confidence: 99.9% Identity: 17% Fold: Multidrug efflux transporter AcrB transmembrane domain Superfamily: Multidrug efflux transporter AcrB transmembrane domain Family: Multidrug efflux transporter AcrB transmembrane domain Phyre2 PDB 3jd8 chain A Region: 344 - 540 Aligned: 191 Modelled: 193 Confidence: 99.9% Identity: 12% PDB header:membrane protein Chain: A: PDB Molecule:niemann-pick c1 protein; PDBTitle: cryo-em structure of the full-length human npc1 at 4.4 angstrom Phyre2 PDB 3aqo chain D Region: 47 - 374 Aligned: 216 Modelled: 223 Confidence: 99.9% Identity: 31% PDB header:membrane protein Chain: D: PDB Molecule:probable secdf protein-export membrane protein; PDBTitle: structure and function of a membrane component secdf that enhances2 protein export Phyre2 1 c3aqpB_ 2 c5xamA_ 3 c5lq3F_ 4 c2v50A_ 5 c6owsB_ 6 c3k07A_ 7 c4mt1A_ 8 c1oy8A_ 9 c6csxA_ 10 c4k0eA_ 11 c4k0eC_ 12 c5mg3D_ 13 c5mg3F_ 14 c6ajjA_ 15 c6dmoA_ 16 c5khnB_ 17 d1iwga8 18 d1iwga7 19 c3jd8A_ 20 c3aqoD_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69