1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	R	S	E	R	L	R	W	L	V	A	A	E	G	P	F	A	S	V	Y	F	D	D	S	H	D	T	L	D	A	V	E	R	R	E	A	T	W	R	D	V	R	K	H	L	E	S	R	D	A	K	Q	E	L	I	D	S	L	E	E
Secondary structure
SS confidence
Disorder		?	?																								?		?																				?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		A	V	R	D	S	R	P	A	V	G	Q	R	G	R	A	L	I	A	T	G	E	Q	V	L	V	N	E	H	L	I	G	P	P	P	A	T	V	I	R	L	S	D	Y	P	Y	V	V	P	L	I	D	L	E	M	R	R	P	T	Y	V
Secondary structure
SS confidence
Disorder					?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		F	A	A	V	D	H	T	G	A	D	V	K	L	Y	Q	G	A	T	I	S	S	T	K	I	D	G	V	G	Y	P	V	H	K	P	V	T	A	G	W	N	G	Y	G	D	F	Q	H	T	T	E	E	A	I	R	M	N	C	R	A	V
Secondary structure
SS confidence
Disorder																								?		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		A	D	H	L	T	R	L	V	D	A	A	D	P	E	V	V	F	V	S	G	E	V	R	S	R	T	D	L	L	S	T	L	P	Q	R	V	A	V	R	V	S	Q	L	H	A	G	P	R	K	S	A	L	D	E	E	E	I	W	D	L
Secondary structure
SS confidence
Disorder																																													?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		T	S	A	E	F	T	R	R	R	Y	A	E	I	T	N	V	A	Q	Q	F	E	A	E	I	G	R	G	S	G	L	A	A	Q	G	L	A	E	V	C	A	A	L	R	D	G	D	V	D	T	L	I	V	G	E	L	G	E	A	T	V
Secondary structure
SS confidence
Disorder																								?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		V	T	G	K	A	R	T	T	V	A	R	D	A	D	M	L	S	E	L	G	E	P	V	D	R	V	A	R	A	D	E	A	L	P	F	A	A	I	A	V	G	A	A	L	V	R	D	D	N	R	I	A	P	L	D	G	V	G	A	L
Secondary structure
SS confidence
Disorder							?				?	?	?	?	?		?		?	?	?	?	?	?	?	?	?	?																						?	?	?		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.
Sequence		L	R	Y	A	A	T	N	R	L	G	S	H	R	S
Secondary structure
SS confidence
Disorder							?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 23%) Alpha helix ( 40%) Beta strand ( 19%)

Rank Aligned region

PDB 4af1 chain A Region: 3 - 367 Aligned: 361 Modelled: 365 Confidence: 100.0% Identity: 18% PDB header:hydrolase Chain: A: PDB Molecule:peptide chain release factor subunit 1; PDBTitle: archeal release factor arf1 Phyre2 PDB 1dt9 chain A Region: 3 - 364 Aligned: 354 Modelled: 362 Confidence: 100.0% Identity: 17% PDB header:translation Chain: A: PDB Molecule:protein (eukaryotic peptide chain release factor PDBTitle: the crystal structure of human eukaryotic release factor2 erf1-mechanism of stop codon recognition and peptidyl-trna3 hydrolysis Phyre2 PDB 4crn chain X Region: 3 - 364 Aligned: 359 Modelled: 362 Confidence: 100.0% Identity: 18% PDB header:translation Chain: X: PDB Molecule:erf1 in ribosome-bound erf1-erf3-gdpnp complex; PDBTitle: cryo-em of a pretermination complex with erf1 and erf3 Phyre2 PDB 3agk chain A Region: 3 - 363 Aligned: 319 Modelled: 330 Confidence: 100.0% Identity: 18% PDB header:translation Chain: A: PDB Molecule:peptide chain release factor subunit 1; PDBTitle: crystal structure of archaeal translation termination factor, arf1 Phyre2 PDB 3e1y chain D Region: 5 - 364 Aligned: 294 Modelled: 320 Confidence: 100.0% Identity: 19% PDB header:translation Chain: D: PDB Molecule:eukaryotic peptide chain release factor subunit 1; PDBTitle: crystal structure of human erf1/erf3 complex Phyre2 PDB 3mca chain B Region: 100 - 364 Aligned: 233 Modelled: 236 Confidence: 100.0% Identity: 12% PDB header:translation regulation/hydrolase Chain: B: PDB Molecule:protein dom34; PDBTitle: structure of the dom34-hbs1 complex and implications for its role in2 no-go decay Phyre2 PDB 3agj chain D Region: 114 - 364 Aligned: 217 Modelled: 229 Confidence: 100.0% Identity: 18% PDB header:translation/hydrolase Chain: D: PDB Molecule:protein pelota homolog; PDBTitle: crystal structure of archaeal pelota and gtp-bound ef1 alpha complex Phyre2 PDB 3agj chain B Region: 109 - 364 Aligned: 222 Modelled: 234 Confidence: 100.0% Identity: 18% PDB header:translation/hydrolase Chain: B: PDB Molecule:protein pelota homolog; PDBTitle: crystal structure of archaeal pelota and gtp-bound ef1 alpha complex Phyre2 PDB 3j15 chain A Region: 100 - 364 Aligned: 228 Modelled: 240 Confidence: 100.0% Identity: 20% PDB header:translation/transport protein Chain: A: PDB Molecule:protein pelota; PDBTitle: model of ribosome-bound archaeal pelota and abce1 Phyre2 PDB 3e20 chain C Region: 3 - 364 Aligned: 218 Modelled: 229 Confidence: 99.9% Identity: 21% PDB header:translation Chain: C: PDB Molecule:eukaryotic peptide chain release factor subunit 1; PDBTitle: crystal structure of s.pombe erf1/erf3 complex Phyre2 PDB 3obw chain A Region: 116 - 364 Aligned: 210 Modelled: 210 Confidence: 99.9% Identity: 17% PDB header:hydrolase Chain: A: PDB Molecule:protein pelota homolog; PDBTitle: crystal structure of two archaeal pelotas reveal inter-domain2 structural plasticity Phyre2 PDB 2vgm chain A Region: 100 - 364 Aligned: 225 Modelled: 226 Confidence: 99.9% Identity: 14% PDB header:cell cycle Chain: A: PDB Molecule:dom34; PDBTitle: structure of yeast dom34 : a protein related to translation2 termination factor erf1 and involved in no-go decay. Phyre2 PDB 3ir9 chain A Region: 227 - 367 Aligned: 141 Modelled: 141 Confidence: 99.9% Identity: 20% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:peptide chain release factor subunit 1; PDBTitle: c-terminal domain of peptide chain release factor from methanosarcina2 mazei. Phyre2 PDB 3oby chain B Region: 100 - 364 Aligned: 211 Modelled: 217 Confidence: 99.9% Identity: 22% PDB header:hydrolase Chain: B: PDB Molecule:protein pelota homolog; PDBTitle: crystal structure of archaeoglobus fulgidus pelota reveals inter-2 domain structural plasticity Phyre2 PDB 1dt9 chain A domain 2 Region: 247 - 368 Aligned: 117 Modelled: 122 Confidence: 99.8% Identity: 18% Fold: Bacillus chorismate mutase-like Superfamily: L30e-like Family: ERF1/Dom34 C-terminal domain-like Phyre2 PDB 2qi2 chain A Region: 100 - 364 Aligned: 211 Modelled: 220 Confidence: 99.8% Identity: 17% PDB header:cell cycle Chain: A: PDB Molecule:cell division protein pelota related protein; PDBTitle: crystal structure of the thermoplasma acidophilum pelota2 protein Phyre2 PDB 2ktv chain A Region: 247 - 364 Aligned: 118 Modelled: 118 Confidence: 99.8% Identity: 21% PDB header:translation Chain: A: PDB Molecule:eukaryotic peptide chain release factor subunit 1; PDBTitle: human erf1 c-domain, "open" conformer Phyre2 PDB 3e20 chain H Region: 240 - 364 Aligned: 115 Modelled: 125 Confidence: 99.7% Identity: 26% PDB header:translation Chain: H: PDB Molecule:eukaryotic peptide chain release factor subunit 1; PDBTitle: crystal structure of s.pombe erf1/erf3 complex Phyre2 PDB 1x52 chain A domain 1 Region: 245 - 364 Aligned: 98 Modelled: 98 Confidence: 99.5% Identity: 19% Fold: Bacillus chorismate mutase-like Superfamily: L30e-like Family: ERF1/Dom34 C-terminal domain-like Phyre2 PDB 1dt9 chain A domain 1 Region: 116 - 245 Aligned: 128 Modelled: 130 Confidence: 99.5% Identity: 19% Fold: Ribonuclease H-like motif Superfamily: Translational machinery components Family: ERF1/Dom34 middle domain-like Phyre2 1 c4af1A_ 2 c1dt9A_ 3 c4crnX_ 4 c3agkA_ 5 c3e1yD_ 6 c3mcaB_ 7 c3agjD_ 8 c3agjB_ 9 c3j15A_ 10 c3e20C_ 11 c3obwA_ 12 c2vgmA_ 13 c3ir9A_ 14 c3obyB_ 15 d1dt9a2 16 c2qi2A_ 17 c2ktvA_ 18 c3e20H_ 19 d1x52a1 20 d1dt9a1 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99