1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	P	D	S	G	Q	L	G	A	A	D	T	P	L	R	L	L	S	S	V	H	Y	L	T	D	G	E	L	P	Q	L	Y	D	Y	P	D	D	G	T	W	L	R	A	N	F	I	S	S	L	D	G	G	A	T	V	D	G	T	S	G
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?				?	?				?			?			?																																			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		A	M	A	G	P	G	D	R	F	V	F	N	L	L	R	E	L	A	D	V	I	V	V	G	V	G	T	V	R	I	E	G	Y	S	G	V	R	M	G	V	V	Q	R	Q	H	R	Q	A	R	G	Q	S	E	V	P	Q	L	A	I	V
Secondary structure
SS confidence
Disorder			?																																														?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		T	R	S	G	R	L	D	R	D	M	A	V	F	T	R	T	E	M	A	P	L	V	L	T	T	T	A	V	A	D	D	T	R	Q	R	L	A	G	L	A	E	V	I	A	C	S	G	D	D	P	G	T	V	D	E	A	V	L	V	S
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		Q	L	A	A	R	G	L	R	R	I	L	T	E	G	G	P	T	L	L	G	T	F	V	E	R	D	V	L	D	E	L	C	L	T	I	A	P	Y	V	V	G	G	L	A	R	R	I	V	T	G	P	G	Q	V	L	T	R	M	R	C
Secondary structure
SS confidence
Disorder																																														?			?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.
Sequence		A	H	V	L	T	D	D	S	G	Y	L	Y	T	R	Y	V	K	T
Secondary structure
SS confidence
Disorder																		?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 10%) Alpha helix ( 20%) Beta strand ( 28%)

Rank Aligned region

PDB 4xt6 chain A Region: 13 - 258 Aligned: 237 Modelled: 246 Confidence: 100.0% Identity: 98% PDB header:oxidoreductase Chain: A: PDB Molecule:rv2671; PDBTitle: crystal structure of rv2671 from mycobacterium tuberculosis in complex2 with the tetrahydropteridine ring of tetrahydrofolate (thf) Phyre2 PDB 2p4g chain A Region: 22 - 257 Aligned: 234 Modelled: 236 Confidence: 100.0% Identity: 27% PDB header:oxidoreductase Chain: A: PDB Molecule:hypothetical protein; PDBTitle: crystal structure of a pyrimidine reductase-like protein (dip1392)2 from corynebacterium diphtheriae nctc at 2.30 a resolution Phyre2 PDB 2d5n chain B Region: 7 - 258 Aligned: 235 Modelled: 252 Confidence: 100.0% Identity: 19% PDB header:hydrolase, oxidoreductase Chain: B: PDB Molecule:riboflavin biosynthesis protein ribd; PDBTitle: crystal structure of a bifunctional deaminase and reductase2 involved in riboflavin biosynthesis Phyre2 PDB 2o7p chain A Region: 7 - 258 Aligned: 237 Modelled: 252 Confidence: 100.0% Identity: 22% PDB header:hydrolase, oxidoreductase Chain: A: PDB Molecule:riboflavin biosynthesis protein ribd; PDBTitle: the crystal structure of ribd from escherichia coli in complex with2 the oxidised nadp+ cofactor in the active site of the reductase3 domain Phyre2 PDB 5xux chain C Region: 37 - 258 Aligned: 218 Modelled: 222 Confidence: 100.0% Identity: 24% PDB header:oxidoreductase Chain: C: PDB Molecule:conserved protein; PDBTitle: crystal structure of rib7 from methanosarcina mazei Phyre2 PDB 2hxv chain A Region: 7 - 255 Aligned: 220 Modelled: 247 Confidence: 100.0% Identity: 17% PDB header:biosynthetic protein Chain: A: PDB Molecule:diaminohydroxyphosphoribosylaminopyrimidine deaminase/ 5- PDBTitle: crystal structure of a diaminohydroxyphosphoribosylaminopyrimidine2 deaminase/ 5-amino-6-(5-phosphoribosylamino)uracil reductase (tm1828)3 from thermotoga maritima at 1.80 a resolution Phyre2 PDB 3zpg chain A Region: 7 - 258 Aligned: 227 Modelled: 246 Confidence: 100.0% Identity: 22% PDB header:hydrolase Chain: A: PDB Molecule:ribd; PDBTitle: acinetobacter baumannii ribd, form 2 Phyre2 PDB 2b3z chain A domain 1 Region: 39 - 258 Aligned: 208 Modelled: 220 Confidence: 100.0% Identity: 20% Fold: Dihydrofolate reductase-like Superfamily: Dihydrofolate reductase-like Family: RibD C-terminal domain-like Phyre2 PDB 2azn chain A domain 1 Region: 36 - 257 Aligned: 210 Modelled: 211 Confidence: 100.0% Identity: 20% Fold: Dihydrofolate reductase-like Superfamily: Dihydrofolate reductase-like Family: RibD C-terminal domain-like Phyre2 PDB 4ha7 chain B Region: 28 - 257 Aligned: 191 Modelled: 202 Confidence: 100.0% Identity: 16% PDB header:oxidoreductase Chain: B: PDB Molecule:2,5-diamino-6-ribosylamino-4(3h)-pyrimidinone 5'-phosphate PDBTitle: structural insights into the reduction mechanism of saccharomyces2 cerevisia riboflavin biosynthesis reductase rib7 Phyre2 PDB 2hxv chain A domain 1 Region: 39 - 255 Aligned: 192 Modelled: 215 Confidence: 100.0% Identity: 19% Fold: Dihydrofolate reductase-like Superfamily: Dihydrofolate reductase-like Family: RibD C-terminal domain-like Phyre2 PDB 3kgy chain A Region: 34 - 257 Aligned: 210 Modelled: 223 Confidence: 100.0% Identity: 17% PDB header:oxidoreductase Chain: A: PDB Molecule:bifunctional deaminase-reductase domain protein; PDBTitle: crystal structure of putative dihydrofolate reductase (yp_001636057.1)2 from chloroflexus aurantiacus j-10-fl at 1.50 a resolution Phyre2 PDB 3jtw chain B Region: 41 - 257 Aligned: 163 Modelled: 166 Confidence: 100.0% Identity: 18% PDB header:oxidoreductase Chain: B: PDB Molecule:dihydrofolate reductase; PDBTitle: crystal structure of putative dihydrofolate reductase (yp_805003.1)2 from pediococcus pentosaceus atcc 25745 at 1.90 a resolution Phyre2 PDB 2gd9 chain A Region: 37 - 256 Aligned: 169 Modelled: 182 Confidence: 100.0% Identity: 20% PDB header:oxidoreductase Chain: A: PDB Molecule:hypothetical protein yyap; PDBTitle: crystal structure of a putative dihydrofolate reductase (bsu40760,2 yyap) from bacillus subtilis at 2.30 a resolution Phyre2 PDB 1d1g chain A Region: 41 - 257 Aligned: 160 Modelled: 164 Confidence: 100.0% Identity: 19% Fold: Dihydrofolate reductase-like Superfamily: Dihydrofolate reductase-like Family: Dihydrofolate reductases Phyre2 PDB 2xw7 chain A Region: 36 - 257 Aligned: 168 Modelled: 179 Confidence: 100.0% Identity: 20% PDB header:oxidoreductase Chain: A: PDB Molecule:dihydrofolate reductase; PDBTitle: structure of mycobacterium smegmatis putative reductase ms0308 Phyre2 PDB 3ky8 chain B Region: 41 - 257 Aligned: 168 Modelled: 178 Confidence: 100.0% Identity: 20% PDB header:biosynthetic protein Chain: B: PDB Molecule:putative riboflavin biosynthesis protein; PDBTitle: crystal structure of putative riboflavin biosynthesis protein2 (yp_001092907.1) from shewanella sp. pv-4 at 2.12 a resolution Phyre2 PDB 1sej chain A domain 1 Region: 37 - 257 Aligned: 166 Modelled: 175 Confidence: 99.6% Identity: 16% Fold: Dihydrofolate reductase-like Superfamily: Dihydrofolate reductase-like Family: Dihydrofolate reductases Phyre2 PDB 1vdr chain A Region: 41 - 258 Aligned: 156 Modelled: 164 Confidence: 99.4% Identity: 24% Fold: Dihydrofolate reductase-like Superfamily: Dihydrofolate reductase-like Family: Dihydrofolate reductases Phyre2 PDB 5ecc chain A Region: 41 - 257 Aligned: 152 Modelled: 162 Confidence: 99.3% Identity: 19% PDB header:oxidoreductase/oxidoreductase inhibitor Chain: A: PDB Molecule:dehydrofolate reductase type i; PDBTitle: klebsiella pneumoniae dfra1 complexed with nadph and 6-ethyl-5-(3-(2-2 methoxy-5-(pyridin-4-yl)phenyl)prop-1-yn-1-yl)pyrimidine-2,4-diamine Phyre2 1 c4xt6A_ 2 c2p4gA_ 3 c2d5nB_ 4 c2o7pA_ 5 c5xuxC_ 6 c2hxvA_ 7 c3zpgA_ 8 d2b3za1 9 d2azna1 10 c4ha7B_ 11 d2hxva1 12 c3kgyA_ 13 c3jtwB_ 14 c2gd9A_ 15 d1d1ga_ 16 c2xw7A_ 17 c3ky8B_ 18 d1seja1 19 d1vdra_ 20 c5eccA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99