1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		L	A	A	R	R	G	G	I	R	R	T	D	L	L	R	R	S	G	Q	P	R	G	R	H	R	A	S	A	A	E	S	G	L	T	W	I	S	P	T	L	I	L	V	G	F	S	H	R	G	D	R	R	M	T	E	H	L	S	R	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?				?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?					?	?										?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		T	L	T	L	E	V	D	A	P	L	E	R	A	R	V	A	T	L	G	P	H	L	H	G	V	L	M	E	S	I	P	A	D	Y	V	Q	T	L	H	T	V	P	V	N	P	Y	S	Q	Y	A	L	A	R	S	T	T	S	L	E	W
Secondary structure
SS confidence
Disorder								?	?	?	?	?	?		?	?																																						?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		K	I	S	T	L	T	N	E	A	R	Q	Q	I	V	G	P	I	N	D	A	A	F	A	G	F	R	L	R	A	S	G	I	A	T	Q	V	T	S	R	S	L	E	Q	N	P	L	S	Q	F	A	R	I	F	Y	A	R	P	E	T	R
Secondary structure
SS confidence
Disorder																					?	?	?	?																					?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		K	F	R	V	E	F	L	T	P	T	A	F	K	Q	S	G	E	Y	V	F	W	P	D	P	R	L	V	F	Q	S	L	A	Q	K	Y	G	A	I	V	D	G	E	E	P	D	P	G	L	I	A	E	F	G	Q	S	V	R	L	S	A
Secondary structure
SS confidence
Disorder																																									?			?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		F	R	V	A	S	A	P	F	A	V	G	A	A	R	V	P	G	F	T	G	S	A	T	F	T	V	R	G	V	D	T	F	A	S	Y	I	A	A	L	L	W	F	G	E	F	S	G	C	G	I	K	A	S	M	G	M	G	A	I	R
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.
Sequence		V	Q	P	L	A	P	R	E	K	C	V	P	K	P
Secondary structure
SS confidence
Disorder						?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 19%) Alpha helix ( 29%) Beta strand ( 34%)

Rank Aligned region

PDB 6fjw chain A Region: 59 - 303 Aligned: 235 Modelled: 245 Confidence: 100.0% Identity: 31% PDB header:hydrolase Chain: A: PDB Molecule:cas6 protein; PDBTitle: streptococcus thermophilus cas6 Phyre2 PDB 4c97 chain B Region: 57 - 302 Aligned: 228 Modelled: 246 Confidence: 100.0% Identity: 21% PDB header:hydrolase Chain: B: PDB Molecule:cas6a; PDBTitle: cas6 (ttha0078) h37a mutant Phyre2 PDB 4c9d chain A Region: 62 - 299 Aligned: 238 Modelled: 238 Confidence: 100.0% Identity: 27% PDB header:hydrolase/rna Chain: A: PDB Molecule:cas6b; PDBTitle: cas6 (tthb231) product complex Phyre2 PDB 4ilm chain H Region: 60 - 302 Aligned: 233 Modelled: 243 Confidence: 100.0% Identity: 12% PDB header:hydrolase/rna Chain: H: PDB Molecule:crispr-associated endoribonuclease cas6 2; PDBTitle: crispr rna processing endoribonuclease Phyre2 PDB 6njy chain B Region: 180 - 303 Aligned: 122 Modelled: 123 Confidence: 100.0% Identity: 23% PDB header:rna binding protein Chain: B: PDB Molecule:type iv crispr associated cas6 rna endonuclease; PDBTitle: type iv crispr associated rna endonuclease cas6 - apo form Phyre2 PDB 3i4h chain X Region: 56 - 302 Aligned: 206 Modelled: 233 Confidence: 99.9% Identity: 20% PDB header:hydrolase Chain: X: PDB Molecule:endoribonuclease; PDBTitle: crystal structure of cas6 in pyrococcus furiosus Phyre2 PDB 3qjj chain B Region: 59 - 301 Aligned: 210 Modelled: 235 Confidence: 99.9% Identity: 14% PDB header:immune system/rna Chain: B: PDB Molecule:putative uncharacterized protein ph0350; PDBTitle: one ramp protein binding different rna substrates Phyre2 PDB 5yi6 chain A Region: 59 - 301 Aligned: 206 Modelled: 230 Confidence: 99.9% Identity: 19% PDB header:hydrolase Chain: A: PDB Molecule:crispr-associated endoribonuclease cas6 1; PDBTitle: crispr associated protein cas6 Phyre2 PDB 6dd5 chain B Region: 179 - 302 Aligned: 119 Modelled: 124 Confidence: 98.1% Identity: 18% PDB header:hydrolase Chain: B: PDB Molecule:mmb-1 cas6 fused to maltose binding protein,crispr- PDBTitle: crystal structure of the cas6 domain of marinomonas mediterranea mmb-12 cas6-rt-cas1 fusion protein Phyre2 PDB 4z7l chain B Region: 239 - 300 Aligned: 48 Modelled: 48 Confidence: 96.2% Identity: 19% PDB header:hydrolase/rna Chain: B: PDB Molecule:cas6b; PDBTitle: crystal structure of cas6b Phyre2 PDB 6ae2 chain B Region: 258 - 310 Aligned: 53 Modelled: 53 Confidence: 95.3% Identity: 17% PDB header:rna binding protein Chain: B: PDB Molecule:csm3; PDBTitle: crystal structure of csm3 of the type iii-a crispr-cas effector2 complex Phyre2 PDB 4l6u chain B Region: 182 - 302 Aligned: 112 Modelled: 121 Confidence: 94.8% Identity: 21% PDB header:unknown function Chain: B: PDB Molecule:putative uncharacterized protein; PDBTitle: crystal structure of af1868: cmr1 subunit of the cmr rna silencing2 complex Phyre2 PDB 4n0l chain B Region: 258 - 304 Aligned: 47 Modelled: 47 Confidence: 94.7% Identity: 21% PDB header:rna binding protein Chain: B: PDB Molecule:predicted component of a thermophile-specific dna repair PDBTitle: methanopyrus kandleri csm3 crystal structure Phyre2 PDB 5yjd chain B Region: 258 - 302 Aligned: 45 Modelled: 45 Confidence: 94.7% Identity: 13% PDB header:hydrolase Chain: B: PDB Molecule:csm3; PDBTitle: structural insights into the crispr-cas-associated ribonuclease2 activity of staphylococcus epidermidis csm3 Phyre2 PDB 4qts chain C Region: 258 - 302 Aligned: 45 Modelled: 45 Confidence: 94.0% Identity: 18% PDB header:rna binding protein Chain: C: PDB Molecule:crispr type iii-associated ramp protein csm3; PDBTitle: crystal structure of csm3-csm4 subcomplex in the type iii-a crispr-cas2 interference complex Phyre2 PDB 4w8v chain A Region: 253 - 299 Aligned: 47 Modelled: 47 Confidence: 93.3% Identity: 15% PDB header:rna binding protein Chain: A: PDB Molecule:crispr system cmr subunit cmr6; PDBTitle: crystal structure of cmr6 from pyrococcus furiosus Phyre2 PDB 4w8w chain B Region: 258 - 303 Aligned: 46 Modelled: 46 Confidence: 93.3% Identity: 24% PDB header:hydrolase Chain: B: PDB Molecule:crispr system cmr subunit cmr4; PDBTitle: crystal structure of oligomeric cmr4 from pyrococcus furiosus Phyre2 PDB 6muu chain E Region: 235 - 303 Aligned: 69 Modelled: 69 Confidence: 93.1% Identity: 23% PDB header:rna binding protein/rna Chain: E: PDB Molecule:uncharacterized protein csm4; PDBTitle: cryo-em structure of csm-crrna binary complex in type iii-a crispr-cas2 system Phyre2 PDB 6ifn chain F Region: 258 - 302 Aligned: 45 Modelled: 45 Confidence: 92.9% Identity: 18% PDB header:rna binding protein Chain: F: PDB Molecule:type iii-a crispr-associated ramp protein csm3; PDBTitle: crystal structure of type iii-a crispr csm complex Phyre2 PDB 6mus chain K Region: 258 - 304 Aligned: 47 Modelled: 47 Confidence: 92.8% Identity: 23% PDB header:rna binding protein/rna Chain: K: PDB Molecule:uncharacterized protein csm3; PDBTitle: cryo-em structure of larger csm-crrna-target rna ternary complex in2 type iii-a crispr-cas system Phyre2 1 c6fjwA_ 2 c4c97B_ 3 c4c9dA_ 4 c4ilmH_ 5 c6njyB_ 6 c3i4hX_ 7 c3qjjB_ 8 c5yi6A_ 9 c6dd5B_ 10 c4z7lB_ 11 c6ae2B_ 12 c4l6uB_ 13 c4n0lB_ 14 c5yjdB_ 15 c4qtsC_ 16 c4w8vA_ 17 c4w8wB_ 18 c6muuE_ 19 c6ifnF_ 20 c6musK_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99