1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	R	V	H	A	F	V	D	D	A	L	G	D	L	D	A	V	A	L	A	D	A	I	R	S	G	R	V	G	R	A	D	V	V	E	A	A	I	A	R	A	E	A	V	N	P	A	L	N	A	L	A	Y	A	A	F	D	V	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		R	D	A	A	A	M	G	T	G	Q	E	A	F	F	S	G	V	P	T	F	I	K	D	N	V	D	V	A	G	Q	P	S	M	H	G	T	D	A	W	E	P	Y	A	A	V	A	D	S	E	I	T	R	V	V	L	G	T	G	L	V
Secondary structure
SS confidence
Disorder								?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		S	L	G	K	T	Q	L	S	E	F	G	F	S	A	V	A	E	H	P	R	L	G	P	V	R	N	P	W	N	T	D	Y	T	A	G	A	S	S	S	G	S	G	A	L	V	A	A	G	V	V	P	I	A	H	A	N	D	G	G	G
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		S	I	R	I	P	A	A	C	N	G	L	V	G	L	K	P	S	R	G	R	L	P	L	E	P	E	Y	R	R	L	P	V	G	I	V	A	N	G	V	L	T	R	T	V	R	D	T	A	A	F	Y	R	E	A	E	R	L	W	R	N
Secondary structure
SS confidence
Disorder																														?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		H	Q	L	P	P	V	G	D	V	T	S	P	V	K	Q	R	L	R	I	A	V	V	T	R	S	V	L	R	E	A	S	P	E	V	R	Q	L	T	L	K	L	A	G	L	L	E	E	L	G	H	R	V	E	H	V	D	H	P	P	A
Secondary structure
SS confidence
Disorder									?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		P	A	S	F	V	D	D	F	V	L	Y	W	G	F	L	A	L	A	Q	V	R	S	G	R	R	T	F	G	R	T	F	D	P	T	R	L	D	E	L	T	L	G	L	A	R	H	T	G	R	N	L	H	R	L	P	L	A	I	M	R
Secondary structure
SS confidence
Disorder																																															?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		L	R	M	L	R	R	R	S	V	R	F	F	G	T	Y	D	V	L	L	T	P	T	V	A	E	A	T	P	Q	V	G	Y	L	A	P	T	D	Y	Q	T	V	L	D	R	L	S	S	W	V	V	F	T	P	V	Q	N	V	T	G	V
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.
Sequence		P	A	I	S	L	P	L	A	Q	S	A	D	G	M	P	V	G	M	M	L	S	A	D	T	G	R	E	A	L	L	L	E	L	A	Y	E	L	E	E	A	R	P	W	A	R	I	H	A	P	N	I	A	E
Secondary structure
SS confidence
Disorder																																																			?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 5%) Alpha helix ( 43%) Beta strand ( 7%) TM helix ( 3%) (View membrane topology prediction)

Rank Aligned region

PDB 3h0r chain P Region: 12 - 468 Aligned: 450 Modelled: 457 Confidence: 100.0% Identity: 24% PDB header:ligase Chain: P: PDB Molecule:glutamyl-trna(gln) amidotransferase subunit a; PDBTitle: structure of trna-dependent amidotransferase gatcab from2 aquifex aeolicus Phyre2 PDB 2f2a chain A domain 1 Region: 11 - 468 Aligned: 451 Modelled: 458 Confidence: 100.0% Identity: 23% Fold: Amidase signature (AS) enzymes Superfamily: Amidase signature (AS) enzymes Family: Amidase signature (AS) enzymes Phyre2 PDB 4wj3 chain A Region: 12 - 469 Aligned: 450 Modelled: 458 Confidence: 100.0% Identity: 24% PDB header:ligase/rna Chain: A: PDB Molecule:glutamyl-trna(gln) amidotransferase subunit a; PDBTitle: crystal structure of the asparagine transamidosome from pseudomonas2 aeruginosa Phyre2 PDB 6dii chain H Region: 2 - 473 Aligned: 466 Modelled: 472 Confidence: 100.0% Identity: 23% PDB header:hydrolase Chain: H: PDB Molecule:fatty acid amide hydrolase; PDBTitle: structure of arabidopsis fatty acid amide hydrolase in complex with2 methyl linolenyl fluorophosphonate Phyre2 PDB 4gys chain A Region: 14 - 469 Aligned: 444 Modelled: 456 Confidence: 100.0% Identity: 22% PDB header:hydrolase Chain: A: PDB Molecule:allophanate hydrolase; PDBTitle: granulibacter bethesdensis allophanate hydrolase co-crystallized with2 malonate Phyre2 PDB 3kfu chain E Region: 17 - 472 Aligned: 444 Modelled: 456 Confidence: 100.0% Identity: 27% PDB header:ligase/rna Chain: E: PDB Molecule:glutamyl-trna(gln) amidotransferase subunit a; PDBTitle: crystal structure of the transamidosome Phyre2 PDB 3a2q chain A Region: 11 - 471 Aligned: 456 Modelled: 458 Confidence: 100.0% Identity: 33% PDB header:hydrolase Chain: A: PDB Molecule:6-aminohexanoate-cyclic-dimer hydrolase; PDBTitle: structure of 6-aminohexanoate cyclic dimer hydrolase complexed with2 substrate Phyre2 PDB 1mt5 chain A Region: 6 - 460 Aligned: 442 Modelled: 455 Confidence: 100.0% Identity: 23% Fold: Amidase signature (AS) enzymes Superfamily: Amidase signature (AS) enzymes Family: Amidase signature (AS) enzymes Phyre2 PDB 2vya chain B Region: 6 - 462 Aligned: 444 Modelled: 457 Confidence: 100.0% Identity: 24% PDB header:hydrolase Chain: B: PDB Molecule:fatty-acid amide hydrolase 1; PDBTitle: crystal structure of fatty acid amide hydrolase conjugated2 with the drug-like inhibitor pf-750 Phyre2 PDB 5h6s chain B Region: 11 - 470 Aligned: 445 Modelled: 460 Confidence: 100.0% Identity: 22% PDB header:hydrolase Chain: B: PDB Molecule:amidase; PDBTitle: crystal structure of hydrazidase s179a mutant complexed with a2 substrate Phyre2 PDB 5h6t chain B Region: 8 - 470 Aligned: 448 Modelled: 463 Confidence: 100.0% Identity: 22% PDB header:hydrolase Chain: B: PDB Molecule:amidase; PDBTitle: crystal structure of hydrazidase from microbacterium sp. strain hm58-2 Phyre2 PDB 4cp8 chain C Region: 16 - 460 Aligned: 433 Modelled: 445 Confidence: 100.0% Identity: 25% PDB header:hydrolase Chain: C: PDB Molecule:allophanate hydrolase; PDBTitle: structure of the amidase domain of allophanate hydrolase2 from pseudomonas sp strain adp Phyre2 PDB 1m22 chain A Region: 11 - 472 Aligned: 439 Modelled: 448 Confidence: 100.0% Identity: 26% Fold: Amidase signature (AS) enzymes Superfamily: Amidase signature (AS) enzymes Family: Amidase signature (AS) enzymes Phyre2 PDB 6c6g chain A Region: 15 - 466 Aligned: 441 Modelled: 452 Confidence: 100.0% Identity: 27% PDB header:hydrolase Chain: A: PDB Molecule:biuret hydrolase; PDBTitle: an unexpected vestigial protein complex reveals the evolutionary2 origins of an s-triazine catabolic enzyme. inhibitor bound complex. Phyre2 PDB 2dc0 chain A Region: 15 - 462 Aligned: 428 Modelled: 448 Confidence: 100.0% Identity: 28% PDB header:hydrolase Chain: A: PDB Molecule:probable amidase; PDBTitle: crystal structure of amidase Phyre2 PDB 4yj6 chain A Region: 11 - 468 Aligned: 457 Modelled: 458 Confidence: 100.0% Identity: 27% PDB header:hydrolase Chain: A: PDB Molecule:aryl acylamidase; PDBTitle: the crystal structure of a bacterial aryl acylamidase belonging to the2 amidase signature (as) enzymes family Phyre2 PDB 2gi3 chain A domain 1 Region: 14 - 472 Aligned: 438 Modelled: 459 Confidence: 100.0% Identity: 24% Fold: Amidase signature (AS) enzymes Superfamily: Amidase signature (AS) enzymes Family: Amidase signature (AS) enzymes Phyre2 PDB 1ock chain A Region: 15 - 462 Aligned: 410 Modelled: 437 Confidence: 100.0% Identity: 24% Fold: Amidase signature (AS) enzymes Superfamily: Amidase signature (AS) enzymes Family: Amidase signature (AS) enzymes Phyre2 PDB 3a1i chain A Region: 11 - 470 Aligned: 455 Modelled: 460 Confidence: 100.0% Identity: 27% PDB header:hydrolase Chain: A: PDB Molecule:amidase; PDBTitle: crystal structure of rhodococcus sp. n-771 amidase complexed2 with benzamide Phyre2 PDB 4iss chain A Region: 15 - 470 Aligned: 441 Modelled: 456 Confidence: 100.0% Identity: 20% PDB header:hydrolase Chain: A: PDB Molecule:allophanate hydrolase; PDBTitle: semet-substituted kluyveromyces lactis allophanate hydrolase Phyre2 1 c3h0rP_ 2 d2f2aa1 3 c4wj3A_ 4 c6diiH_ 5 c4gysA_ 6 c3kfuE_ 7 c3a2qA_ 8 d1mt5a_ 9 c2vyaB_ 10 c5h6sB_ 11 c5h6tB_ 12 c4cp8C_ 13 d1m22a_ 14 c6c6gA_ 15 c2dc0A_ 16 c4yj6A_ 17 d2gi3a1 18 d1ocka_ 19 c3a1iA_ 20 c4issA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99