1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	K	L	I	T	A	I	V	K	P	F	T	L	D	D	V	K	T	S	L	E	D	A	G	V	L	G	M	T	V	S	E	I	Q	G	Y	G	R	Q	K	G	H	T	E	V	Y	R	G	A	E	Y	S	V	D	F	V	P	K	V	R
Secondary structure
SS confidence
Disorder		?	?																																				?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.
Sequence		I	E	V	V	V	D	D	S	I	V	D	K	V	V	D	S	I	V	R	A	A	R	T	G	K	I	G	D	G	K	V	W	V	S	P	V	D	T	I	V	R	V	R	T	G	E	R	G	H	D	A	L
Secondary structure
SS confidence
Disorder																									?		?																					?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 18%) Alpha helix ( 24%) Beta strand ( 43%)

Rank Aligned region

PDB 4r25 chain A Region: 1 - 112 Aligned: 112 Modelled: 112 Confidence: 100.0% Identity: 36% PDB header:transcription Chain: A: PDB Molecule:nitrogen regulatory pii-like protein; PDBTitle: structure of b. subtilis glnk Phyre2 PDB 2ns1 chain B domain 1 Region: 1 - 112 Aligned: 112 Modelled: 112 Confidence: 100.0% Identity: 54% Fold: Ferredoxin-like Superfamily: GlnB-like Family: Prokaryotic signal transducing protein Phyre2 PDB 3mhy chain C Region: 1 - 112 Aligned: 112 Modelled: 112 Confidence: 100.0% Identity: 56% PDB header:signaling protein Chain: C: PDB Molecule:pii-like protein pz; PDBTitle: a new pii protein structure Phyre2 PDB 1vfj chain A Region: 1 - 112 Aligned: 112 Modelled: 112 Confidence: 100.0% Identity: 44% Fold: Ferredoxin-like Superfamily: GlnB-like Family: Prokaryotic signal transducing protein Phyre2 PDB 2pii chain A Region: 1 - 112 Aligned: 112 Modelled: 112 Confidence: 100.0% Identity: 62% Fold: Ferredoxin-like Superfamily: GlnB-like Family: Prokaryotic signal transducing protein Phyre2 PDB 1qy7 chain A Region: 1 - 112 Aligned: 112 Modelled: 112 Confidence: 100.0% Identity: 62% Fold: Ferredoxin-like Superfamily: GlnB-like Family: Prokaryotic signal transducing protein Phyre2 PDB 2rd5 chain D Region: 1 - 112 Aligned: 112 Modelled: 112 Confidence: 100.0% Identity: 46% PDB header:protein binding Chain: D: PDB Molecule:pii protein; PDBTitle: structural basis for the regulation of n-acetylglutamate kinase by pii2 in arabidopsis thaliana Phyre2 PDB 3ncp chain D Region: 1 - 112 Aligned: 99 Modelled: 112 Confidence: 100.0% Identity: 51% PDB header:signaling protein Chain: D: PDB Molecule:nitrogen regulatory protein p-ii (glnb-2); PDBTitle: glnk2 from archaeoglobus fulgidus Phyre2 PDB 1hwu chain A Region: 1 - 112 Aligned: 99 Modelled: 112 Confidence: 100.0% Identity: 63% Fold: Ferredoxin-like Superfamily: GlnB-like Family: Prokaryotic signal transducing protein Phyre2 PDB 1ul3 chain A Region: 1 - 112 Aligned: 95 Modelled: 95 Confidence: 100.0% Identity: 59% Fold: Ferredoxin-like Superfamily: GlnB-like Family: Prokaryotic signal transducing protein Phyre2 PDB 4ozl chain A Region: 1 - 112 Aligned: 97 Modelled: 97 Confidence: 100.0% Identity: 44% PDB header:signaling protein Chain: A: PDB Molecule:nitrogen regulatory protein p-ii; PDBTitle: glnk2 from haloferax mediterranei complexed with amp Phyre2 PDB 3bzq chain A Region: 1 - 112 Aligned: 97 Modelled: 97 Confidence: 100.0% Identity: 100% PDB header:signaling protein/transcription Chain: A: PDB Molecule:nitrogen regulatory protein p-ii; PDBTitle: high resolution crystal structure of nitrogen regulatory protein2 (rv2919c) of mycobacterium tuberculosis Phyre2 PDB 2j9d chain G Region: 1 - 112 Aligned: 99 Modelled: 112 Confidence: 100.0% Identity: 56% PDB header:membrane transport Chain: G: PDB Molecule:hypothetical nitrogen regulatory pii-like PDBTitle: structure of glnk1 with bound effectors indicates2 regulatory mechanism for ammonia uptake Phyre2 PDB 3o8w chain A Region: 1 - 111 Aligned: 103 Modelled: 111 Confidence: 100.0% Identity: 50% PDB header:signaling protein Chain: A: PDB Molecule:nitrogen regulatory protein p-ii (glnb-1); PDBTitle: archaeoglobus fulgidus glnk1 Phyre2 PDB 4usi chain C Region: 1 - 108 Aligned: 97 Modelled: 96 Confidence: 100.0% Identity: 39% PDB header:signaling protein Chain: C: PDB Molecule:nitrogen regulatory protein pii; PDBTitle: nitrogen regulatory protein pii from chlamydomonas2 reinhardtii in complex with mgatp and 2-oxoglutarate Phyre2 PDB 3l7p chain A Region: 1 - 102 Aligned: 82 Modelled: 86 Confidence: 99.9% Identity: 48% PDB header:transcription Chain: A: PDB Molecule:putative nitrogen regulatory protein pii; PDBTitle: crystal structure of smu.1657c, putative nitrogen regulatory protein2 pii from streptococcus mutans Phyre2 PDB 2cz4 chain A domain 1 Region: 1 - 107 Aligned: 93 Modelled: 107 Confidence: 99.8% Identity: 19% Fold: Ferredoxin-like Superfamily: GlnB-like Family: Prokaryotic signal transducing protein Phyre2 PDB 5d4p chain A Region: 1 - 101 Aligned: 90 Modelled: 101 Confidence: 99.7% Identity: 21% PDB header:signaling protein Chain: A: PDB Molecule:putative nitrogen regulatory protein p-ii glnb; PDBTitle: structure of cpii bound to adp and bicarbonate, from thiomonas2 intermedia k12 Phyre2 PDB 4wk1 chain A Region: 1 - 101 Aligned: 83 Modelled: 84 Confidence: 98.7% Identity: 25% PDB header:signaling protein Chain: A: PDB Molecule:psta; PDBTitle: crystal structure of staphylococcus aureus psta in complex with c-di-2 amp Phyre2 PDB 4rle chain A Region: 1 - 101 Aligned: 84 Modelled: 84 Confidence: 98.6% Identity: 30% PDB header:unknown function Chain: A: PDB Molecule:uncharacterized protein yaaq; PDBTitle: crystal structure of the c-di-amp binding pii-like protein dara Phyre2 1 c4r25A_ 2 d2ns1b1 3 c3mhyC_ 4 d1vfja_ 5 d2piia_ 6 d1qy7a_ 7 c2rd5D_ 8 c3ncpD_ 9 d1hwua_ 10 d1ul3a_ 11 c4ozlA_ 12 c3bzqA_ 13 c2j9dG_ 14 c3o8wA_ 15 c4usiC_ 16 c3l7pA_ 17 d2cz4a1 18 c5d4pA_ 19 c4wk1A_ 20 c4rleA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99