1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	D	Q	F	P	I	M	G	V	P	D	G	G	D	T	A	W	M	L	V	S	S	A	L	V	L	L	M	T	P	G	L	A	F	F	Y	G	G	M	V	R	S	K	S	V	L	N	M	I	M	M	S	I	S	A	M	G	V	V	T
Secondary structure
SS confidence
Disorder		?	?	?	?	?																																				?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		V	L	W	A	L	Y	G	Y	S	I	A	F	G	D	D	V	G	N	I	A	G	N	P	S	Q	Y	W	G	L	K	G	L	I	G	V	N	A	V	A	A	D	P	S	T	Q	T	A	A	V	N	I	P	L	A	G	T	L	P	A	T
Secondary structure
SS confidence
Disorder																	?	?																		?	?			?	?	?	?		?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		V	F	V	A	F	Q	L	M	F	A	I	I	T	V	A	L	I	S	G	A	V	A	D	R	L	K	F	G	A	W	L	L	F	A	G	L	W	A	T	F	V	Y	F	P	V	A	H	W	V	F	A	F	D	G	F	A	A	E	H	G
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		G	W	I	A	N	K	L	H	A	I	D	F	A	G	G	T	A	V	H	I	N	A	G	V	A	A	L	M	L	A	I	V	L	G	K	R	R	G	W	P	A	T	L	F	R	P	H	N	L	P	F	V	M	L	G	A	A	L	L	W
Secondary structure
SS confidence
Disorder																																							?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		F	G	W	Y	G	F	N	A	G	S	A	T	T	A	N	G	V	A	G	A	T	F	V	T	T	T	I	A	T	A	A	A	M	L	G	W	L	L	T	E	R	V	R	D	G	K	A	T	T	L	G	A	A	S	G	I	V	A	G	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		V	A	I	T	P	S	C	S	S	V	N	V	L	G	A	L	A	V	G	V	S	A	G	V	L	C	A	L	A	V	G	L	K	F	K	L	G	F	D	D	S	L	D	V	V	G	V	H	L	V	G	G	L	V	G	T	L	L	V	G
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		L	L	A	A	P	E	A	P	A	I	N	G	V	A	G	V	S	K	G	L	F	Y	G	G	G	F	A	Q	L	E	R	Q	A	L	G	A	C	S	V	L	V	Y	S	G	I	I	T	L	I	L	A	L	I	L	K	F	T	I	G	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.
Sequence		R	L	D	A	E	Q	E	S	T	G	I	D	E	A	E	H	A	E	S	G	Y	D	F	A	V	A	S	G	S	V	L	P	P	R	V	T	V	E	D	S	R	N	G	I	Q	E	R	I	G	Q	K	V	E	A	E	P	K
Secondary structure
SS confidence
Disorder																										?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?						?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 13%) Alpha helix ( 66%) Beta strand ( 0%) TM helix ( 47%) (View membrane topology prediction)

Rank Aligned region

PDB 5aez chain A Region: 8 - 461 Aligned: 428 Modelled: 438 Confidence: 100.0% Identity: 33% PDB header:membrane protein Chain: A: PDB Molecule:mep2; PDBTitle: crystal structure of candida albicans mep2 Phyre2 PDB 5aex chain B Region: 3 - 454 Aligned: 427 Modelled: 436 Confidence: 100.0% Identity: 35% PDB header:membrane protein Chain: B: PDB Molecule:ammonium transporter mep2; PDBTitle: crystal structure of saccharomyces cerevisiae mep2 Phyre2 PDB 2b2h chain A Region: 10 - 441 Aligned: 390 Modelled: 400 Confidence: 100.0% Identity: 43% PDB header:transport protein Chain: A: PDB Molecule:ammonium transporter; PDBTitle: ammonium transporter amt-1 from a. fulgidus (as) Phyre2 PDB 2nuu chain F Region: 8 - 442 Aligned: 401 Modelled: 418 Confidence: 100.0% Identity: 46% PDB header:transport protein/signaling protein Chain: F: PDB Molecule:ammonia channel; PDBTitle: regulating the escherichia coli ammonia channel: the crystal structure2 of the amtb-glnk complex Phyre2 PDB 6eu6 chain A Region: 8 - 436 Aligned: 396 Modelled: 408 Confidence: 100.0% Identity: 29% PDB header:membrane protein Chain: A: PDB Molecule:ammonium transporter; PDBTitle: sensor amt protein Phyre2 PDB 5aex chain J Region: 3 - 443 Aligned: 404 Modelled: 419 Confidence: 100.0% Identity: 34% PDB header:membrane protein Chain: J: PDB Molecule:ammonium transporter mep2; PDBTitle: crystal structure of saccharomyces cerevisiae mep2 Phyre2 PDB 1u7g chain A Region: 8 - 422 Aligned: 381 Modelled: 396 Confidence: 100.0% Identity: 43% Fold: Ammonium transporter Superfamily: Ammonium transporter Family: Ammonium transporter Phyre2 PDB 3c1i chain A Region: 8 - 423 Aligned: 364 Modelled: 399 Confidence: 100.0% Identity: 46% PDB header:transport protein Chain: A: PDB Molecule:ammonia channel; PDBTitle: substrate binding, deprotonation and selectivity at the periplasmic2 entrance of the e. coli ammonia channel amtb Phyre2 PDB 3hd6 chain A Region: 22 - 439 Aligned: 379 Modelled: 388 Confidence: 100.0% Identity: 18% PDB header:membrane protein, transport protein Chain: A: PDB Molecule:ammonium transporter rh type c; PDBTitle: crystal structure of the human rhesus glycoprotein rhcg Phyre2 PDB 3b9y chain A Region: 24 - 472 Aligned: 365 Modelled: 388 Confidence: 100.0% Identity: 22% PDB header:transport protein Chain: A: PDB Molecule:ammonium transporter family rh-like protein; PDBTitle: crystal structure of the nitrosomonas europaea rh protein Phyre2 PDB 4q2e chain A Region: 262 - 349 Aligned: 88 Modelled: 88 Confidence: 89.0% Identity: 13% PDB header:transferase Chain: A: PDB Molecule:phosphatidate cytidylyltransferase; PDBTitle: crystal structure of an intramembrane cdp-dag synthetase central for2 phospholipid biosynthesis (s200c/s258c, active mutant) Phyre2 PDB 5b57 chain B Region: 262 - 435 Aligned: 165 Modelled: 174 Confidence: 87.2% Identity: 16% PDB header:metal transport Chain: B: PDB Molecule:putative hemin abc transport system, membrane protein; PDBTitle: inward-facing conformation of abc heme importer bhuuv from2 burkholderia cenocepacia Phyre2 PDB 4ezd chain B Region: 259 - 336 Aligned: 78 Modelled: 78 Confidence: 76.9% Identity: 13% PDB header:transport protein Chain: B: PDB Molecule:urea transporter 1; PDBTitle: crystal structure of the ut-b urea transporter from bos taurus bound2 to selenourea Phyre2 PDB 4q2g chain A Region: 224 - 349 Aligned: 126 Modelled: 126 Confidence: 63.3% Identity: 12% PDB header:transferase Chain: A: PDB Molecule:phosphatidate cytidylyltransferase; PDBTitle: crystal structure of an intramembrane cdp-dag synthetase central for2 phospholipid biosynthesis (s200c/s223c, inactive mutant) Phyre2 PDB 6gcs chain J Region: 223 - 341 Aligned: 119 Modelled: 119 Confidence: 58.6% Identity: 15% PDB header:oxidoreductase Chain: J: PDB Molecule:nujm subunit; PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 PDB 4g1u chain B Region: 229 - 435 Aligned: 198 Modelled: 207 Confidence: 57.2% Identity: 16% PDB header:transport protein/hydrolase Chain: B: PDB Molecule:hemin transport system permease protein hmuu; PDBTitle: x-ray structure of the bacterial heme transporter hmuuv from yersinia2 pestis Phyre2 PDB 4dbl chain B Region: 262 - 435 Aligned: 166 Modelled: 174 Confidence: 56.6% Identity: 17% PDB header:transport protein Chain: B: PDB Molecule:vitamin b12 import system permease protein btuc; PDBTitle: crystal structure of e159q mutant of btucdf Phyre2 PDB 3k3g chain A Region: 259 - 338 Aligned: 80 Modelled: 80 Confidence: 39.7% Identity: 20% PDB header:transport protein Chain: A: PDB Molecule:urea transporter; PDBTitle: crystal structure of the urea transporter from desulfovibrio vulgaris2 bound to 1,3-dimethylurea Phyre2 PDB 2dex chain X domain 1 Region: 166 - 177 Aligned: 12 Modelled: 12 Confidence: 37.6% Identity: 25% Fold: Common fold of diphtheria toxin/transcription factors/cytochrome f Superfamily: Peptidylarginine deiminase Pad4, middle domain Family: Peptidylarginine deiminase Pad4, middle domain Phyre2 PDB 6mhq chain E Region: 24 - 70 Aligned: 47 Modelled: 47 Confidence: 28.5% Identity: 21% PDB header:membrane protein Chain: E: PDB Molecule:gap junction alpha-3 protein, connexin-46; PDBTitle: structure of connexin-46 intercellular gap junction channel at 3.42 angstrom resolution by cryoem Phyre2 1 c5aezA_ 2 c5aexB_ 3 c2b2hA_ 4 c2nuuF_ 5 c6eu6A_ 6 c5aexJ_ 7 d1u7ga_ 8 c3c1iA_ 9 c3hd6A_ 10 c3b9yA_ 11 c4q2eA_ 12 c5b57B_ 13 c4ezdB_ 14 c4q2gA_ 15 c6gcsJ_ 16 c4g1uB_ 17 c4dblB_ 18 c3k3gA_ 19 d2dexx1 20 c6mhqE_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63