1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	P	V	T	D	R	S	V	P	S	L	L	Q	E	R	A	D	Q	Q	P	D	S	T	A	Y	T	Y	I	D	Y	G	S	D	P	K	G	F	A	D	S	L	T	W	S	Q	V	Y	S	R	A	C	I	I	A	E	E	L	K	L	C
Secondary structure
SS confidence
Disorder		?	?	?	?	?																												?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		G	L	P	G	D	R	V	A	V	L	A	P	Q	G	L	E	Y	V	L	A	F	L	G	A	L	Q	A	G	F	I	A	V	P	L	S	T	P	Q	Y	G	I	H	D	D	R	V	S	A	V	L	Q	D	S	K	P	V	A	I	L	T
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		T	S	S	V	V	G	D	V	T	K	Y	A	A	S	H	D	G	Q	P	A	P	V	V	V	E	V	D	L	L	D	L	D	S	P	R	Q	M	P	A	F	S	R	Q	H	T	G	A	A	Y	L	Q	Y	T	S	G	S	T	R	T	P
Secondary structure
SS confidence
Disorder																																	?		?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		A	G	V	I	V	S	H	T	N	V	I	A	N	V	T	Q	S	M	Y	G	Y	F	G	D	P	A	K	I	P	T	G	T	V	V	S	W	L	P	L	Y	H	D	M	G	L	I	L	G	I	C	A	P	L	V	A	R	R	R	A	M
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		L	M	S	P	M	S	F	L	R	R	P	A	R	W	M	Q	L	L	A	T	S	G	R	C	F	S	A	A	P	N	F	A	F	E	L	A	V	R	R	T	S	D	Q	D	M	A	G	L	D	L	R	D	V	V	G	I	V	S	G	S
Secondary structure
SS confidence
Disorder																																														?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		E	R	I	H	V	A	T	V	R	R	F	I	E	R	F	A	P	Y	N	L	S	P	T	A	I	R	P	S	Y	G	L	A	E	A	T	L	Y	V	A	A	P	E	A	G	A	A	P	K	T	V	R	F	D	Y	E	Q	L	T	A	G
Secondary structure
SS confidence
Disorder																																																											?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		Q	A	R	P	C	G	T	D	G	S	V	G	T	E	L	I	S	Y	G	S	P	D	P	S	S	V	R	I	V	N	P	E	T	M	V	E	N	P	P	G	V	V	G	E	I	W	V	H	G	D	H	V	T	M	G	Y	W	Q	K	P
Secondary structure
SS confidence
Disorder					?	?		?	?	?	?	?	?																																										?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		K	Q	T	A	Q	V	F	D	A	K	L	V	D	P	A	P	A	A	P	E	G	P	W	L	R	T	G	D	L	G	V	I	S	D	G	E	L	F	I	M	G	R	I	K	D	L	L	I	V	D	G	R	N	H	Y	P	D	D	I	E
Secondary structure
SS confidence
Disorder														?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		A	T	I	Q	E	I	T	G	G	R	A	A	A	I	A	V	P	D	D	I	T	E	Q	L	V	A	I	I	E	F	K	R	R	G	S	T	A	E	E	V	M	L	K	L	R	S	V	K	R	E	V	T	S	A	I	S	K	S	H	S
Secondary structure
SS confidence
Disorder																																				?	?	?	?	?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560	.	.	.	.	.	.	.	.	.	570	.	.	.	.	.	.	.	.	.	580	.	.	.
Sequence		L	R	V	A	D	L	V	L	V	S	P	G	S	I	P	I	T	T	S	G	K	I	R	R	S	A	C	V	E	R	Y	R	S	D	G	F	K	R	L	D	V	A	V
Secondary structure
SS confidence
Disorder														?		?	?																						?		?		?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 8%) Alpha helix ( 33%) Beta strand ( 25%) TM helix ( 7%) (View membrane topology prediction)

Rank Aligned region

PDB 6eqo chain B Region: 9 - 575 Aligned: 526 Modelled: 544 Confidence: 100.0% Identity: 20% PDB header:oxidoreductase Chain: B: PDB Molecule:acetyl-coenzyme a synthetase; PDBTitle: tri-functional propionyl-coa synthase of erythrobacter sp. nap1 with2 bound nadp+ and phosphomethylphosphonic acid adenylate ester Phyre2 PDB 5es8 chain A Region: 5 - 569 Aligned: 476 Modelled: 539 Confidence: 100.0% Identity: 22% PDB header:ligase Chain: A: PDB Molecule:linear gramicidin synthetase subunit a; PDBTitle: crystal structure of the initiation module of lgra in the thiolation2 state Phyre2 PDB 5gxd chain A Region: 11 - 579 Aligned: 517 Modelled: 546 Confidence: 100.0% Identity: 19% PDB header:lyase Chain: A: PDB Molecule:amp-dependent synthetase and ligase; PDBTitle: structure of acryloyl-coa lyase prpe from dinoroseobacter shibae dfl2 12 Phyre2 PDB 6p1j chain A Region: 2 - 567 Aligned: 500 Modelled: 542 Confidence: 100.0% Identity: 26% PDB header:biosynthetic protein Chain: A: PDB Molecule:txo2; PDBTitle: the structure of condensation and adenylation domains of teixobactin-2 producing nonribosomal peptide synthetase txo2 serine module Phyre2 PDB 5u89 chain A Region: 2 - 575 Aligned: 490 Modelled: 542 Confidence: 100.0% Identity: 25% PDB header:hydrolase/inhibitor Chain: A: PDB Molecule:amino acid adenylation domain protein; PDBTitle: crystal structure of a cross-module fragment from the dimodular nrps2 dhbf Phyre2 PDB 5ifi chain A Region: 7 - 583 Aligned: 528 Modelled: 552 Confidence: 100.0% Identity: 19% PDB header:ligase Chain: A: PDB Molecule:acetyl-coenzyme a synthetase; PDBTitle: crystal structure of acetyl-coa synthetase in complex with adenosine-2 5'-propylphosphate from cryptococcus neoformans h99 Phyre2 PDB 1pg4 chain A Region: 7 - 575 Aligned: 516 Modelled: 544 Confidence: 100.0% Identity: 18% Fold: Acetyl-CoA synthetase-like Superfamily: Acetyl-CoA synthetase-like Family: Acetyl-CoA synthetase-like Phyre2 PDB 5ey8 chain D Region: 1 - 578 Aligned: 569 Modelled: 578 Confidence: 100.0% Identity: 36% PDB header:ligase Chain: D: PDB Molecule:acyl-coa synthase; PDBTitle: structure of fadd32 from mycobacterium smegmatis complexed to ampc20 Phyre2 PDB 5ja2 chain A Region: 2 - 569 Aligned: 501 Modelled: 548 Confidence: 100.0% Identity: 23% PDB header:ligase Chain: A: PDB Molecule:enterobactin synthase component f; PDBTitle: entf, a terminal nonribosomal peptide synthetase module bound to the2 non-native mbth-like protein pa2412 Phyre2 PDB 5wmm chain A Region: 7 - 567 Aligned: 475 Modelled: 513 Confidence: 100.0% Identity: 26% PDB header:biosynthetic protein Chain: A: PDB Molecule:nrps; PDBTitle: crystal structure of an adenylation domain interrupted by a2 methylation domain (ama4) from nonribosomal peptide synthetase tios Phyre2 PDB 6n8e chain A Region: 2 - 569 Aligned: 502 Modelled: 542 Confidence: 100.0% Identity: 20% PDB header:hydrolase Chain: A: PDB Molecule:holo-obif1; PDBTitle: crystal structure of holo-obif1, a five domain nonribosomal peptide2 synthetase from burkholderia diffusa Phyre2 PDB 2vsq chain A Region: 1 - 569 Aligned: 495 Modelled: 536 Confidence: 100.0% Identity: 25% PDB header:ligase Chain: A: PDB Molecule:surfactin synthetase subunit 3; PDBTitle: structure of surfactin a synthetase c (srfa-c), a nonribosomal peptide2 synthetase termination module Phyre2 PDB 5msd chain A Region: 4 - 583 Aligned: 519 Modelled: 519 Confidence: 100.0% Identity: 18% PDB header:oxidoreductase Chain: A: PDB Molecule:carboxylic acid reductase; PDBTitle: structure of the a domain of carboxylic acid reductase (car) from2 nocardia iowensis in complex with amp and benzoic acid Phyre2 PDB 1ry2 chain A Region: 7 - 574 Aligned: 511 Modelled: 544 Confidence: 100.0% Identity: 21% Fold: Acetyl-CoA synthetase-like Superfamily: Acetyl-CoA synthetase-like Family: Acetyl-CoA synthetase-like Phyre2 PDB 4wd1 chain A Region: 7 - 582 Aligned: 517 Modelled: 543 Confidence: 100.0% Identity: 15% PDB header:ligase Chain: A: PDB Molecule:acetoacetate-coa ligase; PDBTitle: acetoacetyl-coa synthetase from streptomyces lividans Phyre2 PDB 3kxw chain A Region: 5 - 579 Aligned: 550 Modelled: 575 Confidence: 100.0% Identity: 33% PDB header:ligase Chain: A: PDB Molecule:saframycin mx1 synthetase b; PDBTitle: the crystal structure of fatty acid amp ligase from legionella2 pneumophila Phyre2 PDB 5mst chain A Region: 2 - 583 Aligned: 521 Modelled: 548 Confidence: 100.0% Identity: 17% PDB header:oxidoreductase Chain: A: PDB Molecule:thioester reductase domain-containing protein; PDBTitle: structure of the a domain of carboxylic acid reductase (car) from2 segniliparus rugosus in complex with amp and a co-purified carboxylic3 acid Phyre2 PDB 3gqw chain B Region: 7 - 579 Aligned: 536 Modelled: 573 Confidence: 100.0% Identity: 29% PDB header:ligase Chain: B: PDB Molecule:fatty acid amp ligase; PDBTitle: crystal structure of a fatty acid amp ligase from e. coli with an acyl2 adenylate product bound Phyre2 PDB 4zxi chain A Region: 2 - 577 Aligned: 485 Modelled: 549 Confidence: 100.0% Identity: 22% PDB header:biosynthetic protein Chain: A: PDB Molecule:tyrocidine synthetase 3; PDBTitle: crystal structure of holo-ab3403 a four domain nonribosomal peptide2 synthetase bound to amp and glycine Phyre2 PDB 4r0m chain B Region: 1 - 580 Aligned: 533 Modelled: 548 Confidence: 100.0% Identity: 24% PDB header:ligase Chain: B: PDB Molecule:mcyg protein; PDBTitle: structure of mcyg a-pcp complexed with phenylalanyl-adenylate Phyre2 1 c6eqoB_ 2 c5es8A_ 3 c5gxdA_ 4 c6p1jA_ 5 c5u89A_ 6 c5ifiA_ 7 d1pg4a_ 8 c5ey8D_ 9 c5ja2A_ 10 c5wmmA_ 11 c6n8eA_ 12 c2vsqA_ 13 c5msdA_ 14 d1ry2a_ 15 c4wd1A_ 16 c3kxwA_ 17 c5mstA_ 18 c3gqwB_ 19 c4zxiA_ 20 c4r0mB_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102