1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	F	P	G	S	V	I	R	K	L	S	H	S	E	E	V	F	A	Q	Y	E	V	F	T	S	M	T	I	Q	L	R	G	V	I	D	V	D	A	L	S	D	A	F	D	A	L	L	E	T	H	P	V	L	A	S	H	L	E	Q	S
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?			?				?	?			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		S	D	G	G	W	N	L	V	A	D	D	L	L	H	S	G	I	C	V	I	D	G	T	A	A	T	N	G	S	P	S	G	N	A	E	L	R	L	D	Q	S	V	S	L	L	H	L	Q	L	I	L	R	E	G	G	A	E	L	T	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		Y	L	H	H	C	M	A	D	G	H	H	G	A	V	L	V	D	E	L	F	S	R	Y	T	D	A	V	T	T	G	D	P	G	P	I	T	P	Q	P	T	P	L	S	M	E	A	V	L	A	Q	R	G	I	R	K	Q	G	L	S	G
Secondary structure
SS confidence
Disorder																													?		?	?	?																						?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		A	E	R	F	M	S	V	M	Y	A	Y	E	I	P	A	T	E	T	P	A	V	L	A	H	P	G	L	P	Q	A	V	P	V	T	R	L	W	L	S	K	Q	Q	T	S	D	L	M	A	F	G	R	E	H	R	L	S	L	N	A	V
Secondary structure
SS confidence
Disorder																										?		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		V	A	A	A	I	L	L	T	E	W	Q	L	R	N	T	P	H	V	P	I	P	Y	V	Y	P	V	D	L	R	F	V	L	A	P	P	V	A	P	T	E	A	T	N	L	L	G	A	A	S	Y	L	A	E	I	G	P	N	T	D	I
Secondary structure
SS confidence
Disorder																																								?																	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		V	D	L	A	S	D	I	V	A	T	L	R	A	D	L	A	N	G	V	I	Q	Q	S	G	L	H	F	G	T	A	F	E	G	T	P	P	G	L	P	P	L	V	F	C	T	D	A	T	S	F	P	T	M	R	T	P	P	G	L	E
Secondary structure
SS confidence
Disorder																												?	?	?	?	?	?	?	?	?	?	?															?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		I	E	D	I	K	G	Q	F	Y	C	S	I	S	V	P	L	D	L	Y	S	C	A	V	Y	A	G	Q	L	I	I	E	H	H	G	H	I	A	E	P	G	K	S	L	E	A	I	R	S	L	L	C	T	V	P	S	E	Y	G	W	I
Secondary structure
SS confidence
Disorder							?	?				?					?																																									?	?	?	?
Disorder confidence
		.	.
Sequence		M	E
Secondary structure
SS confidence
Disorder		?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 11%) Alpha helix ( 36%) Beta strand ( 21%)

Rank Aligned region

PDB 1q9j chain A Region: 1 - 418 Aligned: 388 Modelled: 400 Confidence: 100.0% Identity: 98% PDB header:ligase Chain: A: PDB Molecule:polyketide synthase associated protein 5; PDBTitle: structure of polyketide synthase associated protein 5 from2 mycobacterium tuberculosis Phyre2 PDB 6n8e chain A Region: 1 - 413 Aligned: 401 Modelled: 413 Confidence: 100.0% Identity: 12% PDB header:hydrolase Chain: A: PDB Molecule:holo-obif1; PDBTitle: crystal structure of holo-obif1, a five domain nonribosomal peptide2 synthetase from burkholderia diffusa Phyre2 PDB 2vsq chain A Region: 1 - 415 Aligned: 404 Modelled: 415 Confidence: 100.0% Identity: 10% PDB header:ligase Chain: A: PDB Molecule:surfactin synthetase subunit 3; PDBTitle: structure of surfactin a synthetase c (srfa-c), a nonribosomal peptide2 synthetase termination module Phyre2 PDB 6p1j chain A Region: 1 - 413 Aligned: 405 Modelled: 413 Confidence: 100.0% Identity: 16% PDB header:biosynthetic protein Chain: A: PDB Molecule:txo2; PDBTitle: the structure of condensation and adenylation domains of teixobactin-2 producing nonribosomal peptide synthetase txo2 serine module Phyre2 PDB 4zxi chain A Region: 2 - 415 Aligned: 402 Modelled: 406 Confidence: 100.0% Identity: 11% PDB header:biosynthetic protein Chain: A: PDB Molecule:tyrocidine synthetase 3; PDBTitle: crystal structure of holo-ab3403 a four domain nonribosomal peptide2 synthetase bound to amp and glycine Phyre2 PDB 5ja2 chain A Region: 14 - 415 Aligned: 390 Modelled: 397 Confidence: 100.0% Identity: 14% PDB header:ligase Chain: A: PDB Molecule:enterobactin synthase component f; PDBTitle: entf, a terminal nonribosomal peptide synthetase module bound to the2 non-native mbth-like protein pa2412 Phyre2 PDB 4zxj chain A Region: 2 - 415 Aligned: 404 Modelled: 414 Confidence: 100.0% Identity: 15% PDB header:biosynthetic protein Chain: A: PDB Molecule:enterobactin synthase component f; PDBTitle: crystal structure of holo-entf a nonribosomal peptide synthetase in2 the thioester-forming conformation Phyre2 PDB 5u89 chain A Region: 2 - 413 Aligned: 400 Modelled: 412 Confidence: 100.0% Identity: 12% PDB header:hydrolase/inhibitor Chain: A: PDB Molecule:amino acid adenylation domain protein; PDBTitle: crystal structure of a cross-module fragment from the dimodular nrps2 dhbf Phyre2 PDB 6cgo chain B Region: 1 - 413 Aligned: 402 Modelled: 408 Confidence: 100.0% Identity: 12% PDB header:biosynthetic protein Chain: B: PDB Molecule:condensation domain protein; PDBTitle: molecular basis for condensation domain-mediated chain release from2 the enacyloxin polyketide synthase Phyre2 PDB 5t81 chain A Region: 1 - 419 Aligned: 408 Modelled: 419 Confidence: 100.0% Identity: 12% PDB header:biosynthetic protein Chain: A: PDB Molecule:epob; PDBTitle: rhombohedral crystal form of the epob nrps cyclization-docking2 bidomain from sorangium cellulosum Phyre2 PDB 4znm chain B Region: 3 - 413 Aligned: 404 Modelled: 406 Confidence: 100.0% Identity: 11% PDB header:ligase Chain: B: PDB Molecule:c-domain type ii peptide synthetase; PDBTitle: crystal structure of sgcc5 protein from streptomyces globisporus (apo2 form) Phyre2 PDB 6aef chain B Region: 2 - 415 Aligned: 407 Modelled: 414 Confidence: 100.0% Identity: 11% PDB header:transferase Chain: B: PDB Molecule:polyketide synthase associated protein papa2; PDBTitle: papa2 acyl transferase Phyre2 PDB 2jgp chain A Region: 1 - 413 Aligned: 401 Modelled: 408 Confidence: 100.0% Identity: 13% PDB header:ligase Chain: A: PDB Molecule:tyrocidine synthetase 3; PDBTitle: structure of the tycc5-6 pcp-c bidomain of the tyrocidine synthetase2 tycc Phyre2 PDB 5t3e chain A Region: 2 - 413 Aligned: 404 Modelled: 412 Confidence: 100.0% Identity: 8% PDB header:ligase Chain: A: PDB Molecule:bacillamide synthetase heterocyclization domain; PDBTitle: crystal structure of a nonribosomal peptide synthetase2 heterocyclization domain. Phyre2 PDB 4tx3 chain B Region: 1 - 413 Aligned: 406 Modelled: 413 Confidence: 100.0% Identity: 12% PDB header:oxidoreductase Chain: B: PDB Molecule:peptide synthetase, module 7; PDBTitle: complex of the x-domain and oxyb from teicoplanin biosynthesis Phyre2 PDB 5m6p chain B Region: 2 - 417 Aligned: 410 Modelled: 416 Confidence: 100.0% Identity: 13% PDB header:isomerase Chain: B: PDB Molecule:tyrocidine synthase 2; PDBTitle: crystal structure of the epimerization domain from module 3 of2 tyrocidine synthetase b, tycb3(e) Phyre2 PDB 4jn3 chain B Region: 2 - 413 Aligned: 397 Modelled: 399 Confidence: 100.0% Identity: 13% PDB header:biosynthetic protein Chain: B: PDB Molecule:cda peptide synthetase i; PDBTitle: crystal structures of the first condensation domain of the cda2 synthetase Phyre2 PDB 2xhg chain A Region: 1 - 415 Aligned: 409 Modelled: 415 Confidence: 100.0% Identity: 12% PDB header:isomerase Chain: A: PDB Molecule:tyrocidine synthetase a; PDBTitle: crystal structure of the epimerization domain from the initiation2 module of tyrocidine biosynthesis Phyre2 PDB 6m7l chain B Region: 2 - 413 Aligned: 399 Modelled: 412 Confidence: 100.0% Identity: 12% PDB header:biosynthetic protein Chain: B: PDB Molecule:putative non-ribosomal peptide synthetase; PDBTitle: complex of oxya with the x-domain from gpa biosynthesis Phyre2 PDB 6ozv chain A Region: 3 - 413 Aligned: 387 Modelled: 398 Confidence: 100.0% Identity: 17% PDB header:biosynthetic protein Chain: A: PDB Molecule:txo1; PDBTitle: the structure of condensation and adenylation domains of teixobactin-2 producing nonribosomal peptide synthetase txo1 serine module in3 complex with amp Phyre2 1 c1q9jA_ 2 c6n8eA_ 3 c2vsqA_ 4 c6p1jA_ 5 c4zxiA_ 6 c5ja2A_ 7 c4zxjA_ 8 c5u89A_ 9 c6cgoB_ 10 c5t81A_ 11 c4znmB_ 12 c6aefB_ 13 c2jgpA_ 14 c5t3eA_ 15 c4tx3B_ 16 c5m6pB_ 17 c4jn3B_ 18 c2xhgA_ 19 c6m7lB_ 20 c6ozvA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99