1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	T	D	I	I	R	S	D	A	A	T	L	A	A	K	I	A	I	K	E	V	S	S	A	E	I	T	R	A	C	L	D	Q	I	E	A	T	D	E	T	Y	H	A	F	L	H	V	A	A	D	E	A	L	A	A	A	A	A	I	D
Secondary structure
SS confidence
Disorder		?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		K	Q	V	A	A	G	E	P	L	P	S	A	L	A	G	V	P	L	A	L	K	D	V	F	T	T	S	D	M	P	T	T	C	G	S	K	I	L	E	G	W	R	S	P	Y	D	A	T	L	T	A	R	L	R	A	A	G	I	P	I
Secondary structure
SS confidence
Disorder						?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		L	G	K	T	N	M	D	E	F	A	M	G	S	S	T	E	N	S	A	Y	G	P	T	R	N	P	W	N	L	D	R	V	P	G	G	S	G	G	G	S	A	A	A	L	A	A	F	Q	A	P	L	A	I	G	S	D	T	G	G	S
Secondary structure
SS confidence
Disorder																																			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		I	R	Q	P	A	A	L	T	A	T	V	G	V	K	P	T	Y	G	T	V	S	R	Y	G	L	V	A	C	A	S	S	L	D	Q	G	G	P	C	A	R	T	V	L	D	T	A	L	L	H	Q	V	I	A	G	H	D	P	R	D	S
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		T	S	V	D	A	E	V	P	D	V	V	G	A	A	R	A	G	A	V	G	D	L	R	G	V	R	V	G	V	V	R	Q	L	H	G	G	E	G	Y	Q	P	G	V	L	A	S	F	E	A	A	V	E	Q	L	T	A	L	G	A	E
Secondary structure
SS confidence
Disorder								?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		V	S	E	V	D	C	P	H	F	D	H	A	L	A	A	Y	Y	L	I	L	P	S	E	V	S	S	N	L	A	R	F	D	A	M	R	Y	G	L	R	V	G	D	D	G	T	R	S	A	E	E	V	M	A	M	T	R	A	A	G	F
Secondary structure
SS confidence
Disorder																																												?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		G	P	E	V	K	R	R	I	M	I	G	T	Y	A	L	S	A	G	Y	Y	D	A	Y	Y	N	Q	A	Q	K	V	R	T	L	I	A	R	D	L	D	A	A	Y	R	S	V	D	V	L	V	S	P	T	T	P	T	T	A	F	R	L
Secondary structure
SS confidence
Disorder											?	?	?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		G	E	K	V	D	D	P	L	A	M	Y	L	F	D	L	C	T	L	P	L	N	L	A	G	H	C	G	M	S	V	P	S	G	L	S	P	D	D	G	L	P	V	G	L	Q	I	M	A	P	A	L	A	D	D	R	L	Y	R	V	G
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.
Sequence		A	A	Y	E	A	A	R	G	P	L	L	S	A	I
Secondary structure
SS confidence
Disorder										?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 7%) Alpha helix ( 42%) Beta strand ( 10%) TM helix ( 3%) (View membrane topology prediction)

Rank Aligned region

PDB 2f2a chain A domain 1 Region: 3 - 488 Aligned: 476 Modelled: 486 Confidence: 100.0% Identity: 47% Fold: Amidase signature (AS) enzymes Superfamily: Amidase signature (AS) enzymes Family: Amidase signature (AS) enzymes Phyre2 PDB 4wj3 chain A Region: 4 - 489 Aligned: 475 Modelled: 486 Confidence: 100.0% Identity: 51% PDB header:ligase/rna Chain: A: PDB Molecule:glutamyl-trna(gln) amidotransferase subunit a; PDBTitle: crystal structure of the asparagine transamidosome from pseudomonas2 aeruginosa Phyre2 PDB 3h0r chain P Region: 4 - 492 Aligned: 473 Modelled: 481 Confidence: 100.0% Identity: 47% PDB header:ligase Chain: P: PDB Molecule:glutamyl-trna(gln) amidotransferase subunit a; PDBTitle: structure of trna-dependent amidotransferase gatcab from2 aquifex aeolicus Phyre2 PDB 6dii chain H Region: 1 - 493 Aligned: 465 Modelled: 468 Confidence: 100.0% Identity: 27% PDB header:hydrolase Chain: H: PDB Molecule:fatty acid amide hydrolase; PDBTitle: structure of arabidopsis fatty acid amide hydrolase in complex with2 methyl linolenyl fluorophosphonate Phyre2 PDB 1m22 chain A Region: 3 - 493 Aligned: 469 Modelled: 491 Confidence: 100.0% Identity: 33% Fold: Amidase signature (AS) enzymes Superfamily: Amidase signature (AS) enzymes Family: Amidase signature (AS) enzymes Phyre2 PDB 3kfu chain E Region: 9 - 494 Aligned: 463 Modelled: 481 Confidence: 100.0% Identity: 49% PDB header:ligase/rna Chain: E: PDB Molecule:glutamyl-trna(gln) amidotransferase subunit a; PDBTitle: crystal structure of the transamidosome Phyre2 PDB 4gys chain A Region: 5 - 493 Aligned: 446 Modelled: 468 Confidence: 100.0% Identity: 29% PDB header:hydrolase Chain: A: PDB Molecule:allophanate hydrolase; PDBTitle: granulibacter bethesdensis allophanate hydrolase co-crystallized with2 malonate Phyre2 PDB 2vya chain B Region: 3 - 489 Aligned: 467 Modelled: 487 Confidence: 100.0% Identity: 21% PDB header:hydrolase Chain: B: PDB Molecule:fatty-acid amide hydrolase 1; PDBTitle: crystal structure of fatty acid amide hydrolase conjugated2 with the drug-like inhibitor pf-750 Phyre2 PDB 1mt5 chain A Region: 3 - 487 Aligned: 465 Modelled: 485 Confidence: 100.0% Identity: 20% Fold: Amidase signature (AS) enzymes Superfamily: Amidase signature (AS) enzymes Family: Amidase signature (AS) enzymes Phyre2 PDB 4cp8 chain C Region: 7 - 485 Aligned: 442 Modelled: 458 Confidence: 100.0% Identity: 28% PDB header:hydrolase Chain: C: PDB Molecule:allophanate hydrolase; PDBTitle: structure of the amidase domain of allophanate hydrolase2 from pseudomonas sp strain adp Phyre2 PDB 5h6s chain B Region: 3 - 493 Aligned: 443 Modelled: 465 Confidence: 100.0% Identity: 27% PDB header:hydrolase Chain: B: PDB Molecule:amidase; PDBTitle: crystal structure of hydrazidase s179a mutant complexed with a2 substrate Phyre2 PDB 5h6t chain B Region: 3 - 490 Aligned: 440 Modelled: 464 Confidence: 100.0% Identity: 27% PDB header:hydrolase Chain: B: PDB Molecule:amidase; PDBTitle: crystal structure of hydrazidase from microbacterium sp. strain hm58-2 Phyre2 PDB 3a2q chain A Region: 3 - 493 Aligned: 454 Modelled: 470 Confidence: 100.0% Identity: 30% PDB header:hydrolase Chain: A: PDB Molecule:6-aminohexanoate-cyclic-dimer hydrolase; PDBTitle: structure of 6-aminohexanoate cyclic dimer hydrolase complexed with2 substrate Phyre2 PDB 6c6g chain A Region: 7 - 491 Aligned: 448 Modelled: 458 Confidence: 100.0% Identity: 35% PDB header:hydrolase Chain: A: PDB Molecule:biuret hydrolase; PDBTitle: an unexpected vestigial protein complex reveals the evolutionary2 origins of an s-triazine catabolic enzyme. inhibitor bound complex. Phyre2 PDB 4yj6 chain A Region: 2 - 488 Aligned: 462 Modelled: 471 Confidence: 100.0% Identity: 29% PDB header:hydrolase Chain: A: PDB Molecule:aryl acylamidase; PDBTitle: the crystal structure of a bacterial aryl acylamidase belonging to the2 amidase signature (as) enzymes family Phyre2 PDB 2dc0 chain A Region: 7 - 488 Aligned: 429 Modelled: 436 Confidence: 100.0% Identity: 32% PDB header:hydrolase Chain: A: PDB Molecule:probable amidase; PDBTitle: crystal structure of amidase Phyre2 PDB 3a1i chain A Region: 3 - 493 Aligned: 483 Modelled: 491 Confidence: 100.0% Identity: 31% PDB header:hydrolase Chain: A: PDB Molecule:amidase; PDBTitle: crystal structure of rhodococcus sp. n-771 amidase complexed2 with benzamide Phyre2 PDB 2gi3 chain A domain 1 Region: 5 - 493 Aligned: 439 Modelled: 448 Confidence: 100.0% Identity: 45% Fold: Amidase signature (AS) enzymes Superfamily: Amidase signature (AS) enzymes Family: Amidase signature (AS) enzymes Phyre2 PDB 1ock chain A Region: 7 - 488 Aligned: 409 Modelled: 423 Confidence: 100.0% Identity: 30% Fold: Amidase signature (AS) enzymes Superfamily: Amidase signature (AS) enzymes Family: Amidase signature (AS) enzymes Phyre2 PDB 4iss chain A Region: 5 - 493 Aligned: 451 Modelled: 470 Confidence: 100.0% Identity: 25% PDB header:hydrolase Chain: A: PDB Molecule:allophanate hydrolase; PDBTitle: semet-substituted kluyveromyces lactis allophanate hydrolase Phyre2 1 d2f2aa1 2 c4wj3A_ 3 c3h0rP_ 4 c6diiH_ 5 d1m22a_ 6 c3kfuE_ 7 c4gysA_ 8 c2vyaB_ 9 d1mt5a_ 10 c4cp8C_ 11 c5h6sB_ 12 c5h6tB_ 13 c3a2qA_ 14 c6c6gA_ 15 c4yj6A_ 16 c2dc0A_ 17 c3a1iA_ 18 d2gi3a1 19 d1ocka_ 20 c4issA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99