1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	A	P	A	V	T	E	H	S	W	L	P	R	A	T	C	G	V	S	C	V	S	V	G	D	A	A	Q	V	R	R	P	L	V	V	L	R	V	A	L	R	V	M	L	A	L	L	L	V	P	G	V	P	L	V	V	M	P	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?										?		?			?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		P	G	R	T	R	V	Q	R	I	Y	C	R	L	V	L	R	L	F	G	V	R	I	T	V	S	G	S	P	V	R	N	L	R	G	V	L	V	V	S	G	H	V	S	W	L	D	V	F	C	I	G	S	V	L	P	G	S	F	V	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		R	A	D	M	F	T	G	R	T	I	G	I	V	A	R	I	L	K	I	I	P	I	E	R	A	S	L	R	R	L	P	G	V	V	D	T	I	A	R	R	L	R	A	G	Q	T	V	V	A	F	P	E	G	T	T	W	C	G	R	P
Secondary structure
SS confidence
Disorder																										?																																		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		G	D	D	A	G	R	P	A	A	R	A	G	A	G	C	S	H	R	G	C	G	A	F	Y	P	A	M	F	Q	A	A	I	D	A	G	R	P	V	Q	P	L	R	L	T	Y	H	H	V	D	G	T	V	S	T	A	P	A	F	V	G
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?		?																												?		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		D	D	T	L	V	R	S	V	C	R	L	L	T	V	R	R	T	L	A	W	V	R	V	E	S	L	Q	L	P	G	T	D	R	R	N	L	A	R	R	C	Q	S	A	V	L	A	G	A	L	G	Q	S	G	Q	R	P	G	R	R	H
Secondary structure
SS confidence
Disorder															?																																			?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.
Sequence		V	P	A	T
Secondary structure
SS confidence
Disorder		?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 19%) Alpha helix ( 43%) Beta strand ( 13%) TM helix ( 12%) (View membrane topology prediction)

Rank Aligned region

PDB 5kym chain A Region: 28 - 294 Aligned: 225 Modelled: 231 Confidence: 100.0% Identity: 18% PDB header:transferase Chain: A: PDB Molecule:1-acyl-sn-glycerol-3-phosphate acyltransferase; PDBTitle: crystal structure of the 1-acyl-sn-glycerophosphate (lpa)2 acyltransferase, plsc, from thermotoga maritima Phyre2 PDB 5f34 chain A Region: 91 - 294 Aligned: 161 Modelled: 175 Confidence: 99.3% Identity: 12% PDB header:transferase Chain: A: PDB Molecule:phosphatidylinositol mannoside acyltransferase; PDBTitle: crystal structure of membrane associated pata from mycobacterium2 smegmatis in complex with s-hexadecyl coenzyme a - p21 space group Phyre2 PDB 5knk chain B Region: 82 - 294 Aligned: 168 Modelled: 185 Confidence: 99.0% Identity: 13% PDB header:transferase Chain: B: PDB Molecule:lipid a biosynthesis lauroyl acyltransferase; PDBTitle: lipid a secondary acyltransferase lpxm from acinetobacter baumannii2 with catalytic residue substitution (e127a) Phyre2 PDB 1iuq chain A Region: 95 - 304 Aligned: 173 Modelled: 174 Confidence: 97.9% Identity: 21% Fold: Glycerol-3-phosphate (1)-acyltransferase Superfamily: Glycerol-3-phosphate (1)-acyltransferase Family: Glycerol-3-phosphate (1)-acyltransferase Phyre2 PDB 5vrh chain A Region: 143 - 219 Aligned: 59 Modelled: 59 Confidence: 78.5% Identity: 14% PDB header:transferase Chain: A: PDB Molecule:apolipoprotein n-acyltransferase; PDBTitle: apolipoprotein n-acyltransferase c387s active site mutant Phyre2 PDB 4hg3 chain C Region: 148 - 176 Aligned: 29 Modelled: 29 Confidence: 49.0% Identity: 24% PDB header:hydrolase Chain: C: PDB Molecule:probable hydrolase nit2; PDBTitle: structural insights into yeast nit2: wild-type yeast nit2 in complex2 with alpha-ketoglutarate Phyre2 PDB 4cyy chain A Region: 148 - 219 Aligned: 72 Modelled: 72 Confidence: 43.2% Identity: 17% PDB header:hydrolase Chain: A: PDB Molecule:pantetheinase; PDBTitle: the structure of vanin-1: defining the link between metabolic disease,2 oxidative stress and inflammation Phyre2 PDB 2e11 chain B Region: 142 - 219 Aligned: 68 Modelled: 78 Confidence: 38.5% Identity: 10% PDB header:hydrolase Chain: B: PDB Molecule:hydrolase; PDBTitle: the crystal structure of xc1258 from xanthomonas campestris: a cn-2 hydrolase superfamily protein with an arsenic adduct in the active3 site Phyre2 PDB 3wuy chain A Region: 148 - 176 Aligned: 29 Modelled: 29 Confidence: 36.3% Identity: 28% PDB header:hydrolase Chain: A: PDB Molecule:nitrilase; PDBTitle: crystal structure of nit6803 Phyre2 PDB 3hkx chain A Region: 148 - 176 Aligned: 29 Modelled: 29 Confidence: 33.0% Identity: 24% PDB header:hydrolase Chain: A: PDB Molecule:amidase; PDBTitle: crystal structure analysis of an amidase from nesterenkonia sp. Phyre2 PDB 4mxn chain B Region: 152 - 174 Aligned: 23 Modelled: 23 Confidence: 30.5% Identity: 13% PDB header:hydrolase Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of a putative glycosyl hydrolase (parmer_00599) from2 parabacteroides merdae atcc 43184 at 1.95 a resolution Phyre2 PDB 1ems chain B Region: 148 - 220 Aligned: 65 Modelled: 73 Confidence: 28.4% Identity: 11% PDB header:antitumor protein Chain: B: PDB Molecule:nit-fragile histidine triad fusion protein; PDBTitle: crystal structure of the c. elegans nitfhit protein Phyre2 PDB 2vhh chain A Region: 138 - 219 Aligned: 70 Modelled: 82 Confidence: 27.0% Identity: 21% PDB header:hydrolase Chain: A: PDB Molecule:cg3027-pa; PDBTitle: crystal structure of a pyrimidine degrading enzyme from2 drosophila melanogaster Phyre2 PDB 1yj5 chain B Region: 129 - 226 Aligned: 73 Modelled: 77 Confidence: 26.9% Identity: 22% PDB header:transferase Chain: B: PDB Molecule:5' polynucleotide kinase-3' phosphatase catalytic domain; PDBTitle: molecular architecture of mammalian polynucleotide kinase, a dna2 repair enzyme Phyre2 PDB 6i00 chain C Region: 152 - 176 Aligned: 25 Modelled: 25 Confidence: 26.3% Identity: 20% PDB header:hydrolase Chain: C: PDB Molecule:bifunctional nitrilase/nitrile hydratase nit4; PDBTitle: cryo-em informed directed evolution of nitrilase 4 leads to a change2 in quaternary structure. Phyre2 PDB 1k7j chain A Region: 140 - 174 Aligned: 32 Modelled: 35 Confidence: 23.4% Identity: 16% Fold: YrdC/RibB Superfamily: YrdC/RibB Family: YrdC-like Phyre2 PDB 3zvm chain A Region: 129 - 226 Aligned: 73 Modelled: 75 Confidence: 22.1% Identity: 23% PDB header:hydrolase/transferase/dna Chain: A: PDB Molecule:bifunctional polynucleotide phosphatase/kinase; PDBTitle: the structural basis for substrate recognition by mammalian2 polynucleotide kinase 3' phosphatase Phyre2 PDB 6mg6 chain D Region: 145 - 176 Aligned: 32 Modelled: 32 Confidence: 21.8% Identity: 9% PDB header:hydrolase Chain: D: PDB Molecule:carbon-nitrogen hydrolase; PDBTitle: crystal structure of carbon-nitrogen hydrolase from helicobacter2 pylori g27 Phyre2 PDB 6ftq chain A Region: 138 - 219 Aligned: 79 Modelled: 82 Confidence: 21.0% Identity: 11% PDB header:hydrolase Chain: A: PDB Molecule:beta-ureidopropionase; PDBTitle: crystal structure of human beta-ureidopropionase (beta-alanine2 synthase) - mutant t299c Phyre2 PDB 1uf5 chain A Region: 148 - 219 Aligned: 71 Modelled: 72 Confidence: 20.8% Identity: 14% Fold: Carbon-nitrogen hydrolase Superfamily: Carbon-nitrogen hydrolase Family: Carbamilase Phyre2 1 c5kymA_ 2 c5f34A_ 3 c5knkB_ 4 d1iuqa_ 5 c5vrhA_ 6 c4hg3C_ 7 c4cyyA_ 8 c2e11B_ 9 c3wuyA_ 10 c3hkxA_ 11 c4mxnB_ 12 c1emsB_ 13 c2vhhA_ 14 c1yj5B_ 15 c6i00C_ 16 d1k7ja_ 17 c3zvmA_ 18 c6mg6D_ 19 c6ftqA_ 20 d1uf5a_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67