1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	T	T	P	G	R	P	L	T	T	L	D	K	S	D	V	L	A	G	L	F	A	V	W	H	S	L	D	A	L	L	D	G	L	L	E	T	D	W	Q	A	T	S	P	L	P	G	W	D	V	K	A	V	V	S	H	I	I	G	T
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		E	S	F	L	L	G	I	A	A	P	E	P	D	T	D	V	S	A	L	A	H	V	R	N	P	I	G	V	M	N	E	C	W	V	R	H	L	G	T	E	S	G	V	G	L	L	E	R	F	R	A	V	T	S	Q	R	R	K	V	L
Secondary structure
SS confidence
Disorder												?		?	?		?			?		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		A	S	L	S	D	D	E	W	N	A	P	T	T	T	P	S	G	P	D	S	Y	G	R	F	M	R	I	R	I	F	D	C	W	M	H	E	Q	D	I	R	A	A	V	Q	R	P	S	S	D	D	E	L	G	G	P	A	S	P	L	V
Secondary structure
SS confidence
Disorder																?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		L	D	E	I	A	A	T	M	G	F	V	V	G	K	L	A	K	A	P	D	G	S	R	V	L	L	E	L	T	G	P	L	S	R	S	I	R	V	S	V	D	G	R	A	R	V	V	D	D	F	G	G	P	A	P	T	A	T	I	R
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.
Sequence		L	D	G	L	Q	F	T	R	L	A	G	G	R	P	M	S	P	A	R	S	Q	D	V	E	L	G	G	D	K	E	L	A	G	H	I	L	E	R	L	N	F	V	I
Secondary structure
SS confidence
Disorder																		?	?	?	?	?																			?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 10%) Alpha helix ( 52%) Beta strand ( 8%) TM helix ( 6%) (View membrane topology prediction)

Rank Aligned region

PDB 2nsf chain A Region: 6 - 268 Aligned: 231 Modelled: 250 Confidence: 100.0% Identity: 13% PDB header:isomerase Chain: A: PDB Molecule:hypothetical protein cgl3021; PDBTitle: crystal structure of the mycothiol-dependent maleylpyruvate isomerase Phyre2 PDB 2nsf chain A domain 1 Region: 14 - 165 Aligned: 148 Modelled: 152 Confidence: 100.0% Identity: 14% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: Maleylpyruvate isomerase-like Phyre2 PDB 5civ chain A Region: 12 - 145 Aligned: 134 Modelled: 134 Confidence: 99.6% Identity: 16% PDB header:unknown function Chain: A: PDB Molecule:sibling bacteriocin; PDBTitle: sibling lethal factor precursor - dfsb Phyre2 PDB 5cog chain B Region: 12 - 155 Aligned: 144 Modelled: 144 Confidence: 99.5% Identity: 15% PDB header:unknown function Chain: B: PDB Molecule:irc4; PDBTitle: crystal structure of yeast irc4 Phyre2 PDB 1rxq chain A Region: 4 - 161 Aligned: 154 Modelled: 158 Confidence: 99.4% Identity: 15% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: YfiT-like putative metal-dependent hydrolases Phyre2 PDB 4n6c chain B Region: 12 - 152 Aligned: 141 Modelled: 141 Confidence: 99.4% Identity: 12% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of the b1rzq2 protein from streptococcus pneumoniae.2 northeast structural genomics consortium (nesg) target spr36. Phyre2 PDB 2rd9 chain C Region: 10 - 170 Aligned: 157 Modelled: 161 Confidence: 99.2% Identity: 15% PDB header:hydrolase Chain: C: PDB Molecule:bh0186 protein; PDBTitle: crystal structure of a putative yfit-like metal-dependent hydrolase2 (bh0186) from bacillus halodurans c-125 at 2.30 a resolution Phyre2 PDB 6iz2 chain A Region: 8 - 171 Aligned: 142 Modelled: 142 Confidence: 99.2% Identity: 16% PDB header:unknown function Chain: A: PDB Molecule:dinb/yfit family protein; PDBTitle: crystal structure of dinb/yfit protein dr0053 from d. radiodurans r1 Phyre2 PDB 5cof chain A Region: 12 - 146 Aligned: 133 Modelled: 131 Confidence: 99.2% Identity: 14% PDB header:unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of uncharacterised protein q1r1x2 from escherichia2 coli uti89 Phyre2 PDB 3cex chain B Region: 13 - 159 Aligned: 147 Modelled: 147 Confidence: 99.1% Identity: 8% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of the conserved protein of locus ef_3021 from2 enterococcus faecalis Phyre2 PDB 6anr chain A Region: 12 - 146 Aligned: 133 Modelled: 135 Confidence: 99.1% Identity: 17% PDB header:hydrolase Chain: A: PDB Molecule:colibactin self-protection protein clbs; PDBTitle: crystal structure of a self resistance protein clbs from colibactin2 biosynthetic gene cluster Phyre2 PDB 2ou6 chain A domain 1 Region: 3 - 160 Aligned: 155 Modelled: 158 Confidence: 99.0% Identity: 15% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: DinB-like Phyre2 PDB 5cqv chain B Region: 12 - 126 Aligned: 114 Modelled: 115 Confidence: 98.9% Identity: 9% PDB header:unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of uncharacterized protein q8dwv2 from streptococcus2 agalactiae Phyre2 PDB 3dka chain A Region: 14 - 171 Aligned: 136 Modelled: 136 Confidence: 98.8% Identity: 9% PDB header:unknown function Chain: A: PDB Molecule:dinb-like protein; PDBTitle: crystal structure of a dinb-like protein (yjoa, bsu12410) from2 bacillus subtilis at 2.30 a resolution Phyre2 PDB 2yqy chain B Region: 14 - 163 Aligned: 123 Modelled: 126 Confidence: 98.8% Identity: 19% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:hypothetical protein ttha0303; PDBTitle: crystal structure of tt2238, a four-helix bundle protein Phyre2 PDB 2p1a chain A domain 1 Region: 14 - 163 Aligned: 136 Modelled: 136 Confidence: 98.8% Identity: 13% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: DinB-like Phyre2 PDB 3di5 chain A Region: 12 - 168 Aligned: 140 Modelled: 140 Confidence: 98.7% Identity: 16% PDB header:metal binding protein Chain: A: PDB Molecule:dinb-like protein; PDBTitle: crystal structure of a dinb-like protein (bce_4655) from bacillus2 cereus atcc 10987 at 2.01 a resolution Phyre2 PDB 2hkv chain A domain 1 Region: 13 - 163 Aligned: 131 Modelled: 131 Confidence: 98.6% Identity: 13% Fold: DinB/YfiT-like putative metalloenzymes Superfamily: DinB/YfiT-like putative metalloenzymes Family: DinB-like Phyre2 PDB 3e4x chain B Region: 14 - 159 Aligned: 128 Modelled: 128 Confidence: 98.5% Identity: 14% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:apc36150; PDBTitle: crystal structure of putative metal-dependent hydrolases2 apc36150 Phyre2 PDB 2qe9 chain B Region: 23 - 172 Aligned: 132 Modelled: 132 Confidence: 98.4% Identity: 11% PDB header:hydrolase Chain: B: PDB Molecule:uncharacterized protein yiza; PDBTitle: crystal structure of a putative metal-dependent hydrolase (yiza,2 bsu10800) from bacillus subtilis at 1.90 a resolution Phyre2 1 c2nsfA_ 2 d2nsfa1 3 c5civA_ 4 c5cogB_ 5 d1rxqa_ 6 c4n6cB_ 7 c2rd9C_ 8 c6iz2A_ 9 c5cofA_ 10 c3cexB_ 11 c6anrA_ 12 d2ou6a1 13 c5cqvB_ 14 c3dkaA_ 15 c2yqyB_ 16 d2p1aa1 17 c3di5A_ 18 d2hkva1 19 c3e4xB_ 20 c2qe9B_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59