1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	T	T	S	G	T	V	L	A	T	S	I	A	Q	H	W	H	N	F	W	R	G	E	I	G	D	W	I	L	N	R	G	L	R	I	V	M	L	L	I	A	A	V	L	A	A	R	F	V	T	W	L	A	N	R	V	T	R	R	L
Secondary structure
SS confidence
Disorder		?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		D	L	G	F	T	E	S	D	A	L	V	R	S	E	A	T	K	H	R	Q	A	V	A	S	V	I	S	W	V	S	I	V	L	I	Y	V	V	V	V	Y	E	V	I	D	V	L	P	V	P	V	G	A	L	V	G	P	A	A	V	L
Secondary structure
SS confidence
Disorder					?	?	?	?	?	?	?	?	?	?	?	?																															?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		G	A	A	L	G	F	G	A	Q	R	L	V	Q	D	L	L	A	G	F	F	I	I	V	E	K	Q	Y	G	F	G	D	L	V	E	L	S	M	V	G	S	P	E	N	A	A	G	T	V	E	D	V	T	L	R	V	T	K	L	R	S
Secondary structure
SS confidence
Disorder																																								?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		S	E	G	E	V	F	T	V	P	N	G	N	I	V	K	S	V	N	L	S	K	D	W	A	R	A	V	V	D	I	P	V	P	T	S	A	D	L	G	R	V	N	E	V	L	H	Q	E	C	E	H	A	R	H	D	S	L	L	G	E
Secondary structure
SS confidence
Disorder																																																								?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		L	L	L	D	E	P	T	V	M	G	V	E	R	I	E	V	D	T	V	T	L	R	L	V	A	R	T	L	P	G	K	Q	F	E	A	G	R	Q	L	R	V	L	V	I	R	A	L	T	R	A	G	I	V	T	A	A	D	A	R	A
Secondary structure
SS confidence
Disorder		?
Disorder confidence
		.	.	.	.	.	.	.	.
Sequence		A	V	A	E	S	P	E	Q
Secondary structure
SS confidence
Disorder				?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 11%) Alpha helix ( 55%) Beta strand ( 20%) TM helix ( 21%) (View membrane topology prediction)

Rank Aligned region

PDB 3t9n chain G Region: 28 - 307 Aligned: 259 Modelled: 280 Confidence: 100.0% Identity: 27% PDB header:membrane protein Chain: G: PDB Molecule:small-conductance mechanosensitive channel; PDBTitle: crystal structure of a membrane protein Phyre2 PDB 2vv5 chain D Region: 29 - 305 Aligned: 252 Modelled: 277 Confidence: 100.0% Identity: 21% PDB header:membrane protein Chain: D: PDB Molecule:small-conductance mechanosensitive channel; PDBTitle: the open structure of mscs Phyre2 PDB 4hw9 chain E Region: 31 - 305 Aligned: 248 Modelled: 260 Confidence: 100.0% Identity: 18% PDB header:membrane protein Chain: E: PDB Molecule:mechanosensitive channel mscs; PDBTitle: crystal structure of helicobacter pylori mscs (closed state) Phyre2 PDB 5y4o chain A Region: 81 - 305 Aligned: 215 Modelled: 215 Confidence: 100.0% Identity: 16% PDB header:membrane protein Chain: A: PDB Molecule:low conductance mechanosensitive channel ynai; PDBTitle: cryo-em structure of mscs channel, ynai Phyre2 PDB 2vv5 chain A domain 2 Region: 201 - 305 Aligned: 99 Modelled: 105 Confidence: 99.7% Identity: 14% Fold: Ferredoxin-like Superfamily: Mechanosensitive channel protein MscS (YggB), C-terminal domain Family: Mechanosensitive channel protein MscS (YggB), C-terminal domain Phyre2 PDB 2vv5 chain A domain 1 Region: 131 - 200 Aligned: 64 Modelled: 70 Confidence: 99.6% Identity: 31% Fold: Sm-like fold Superfamily: Sm-like ribonucleoproteins Family: Mechanosensitive channel protein MscS (YggB), middle domain Phyre2 PDB 2vv5 chain A domain 3 Region: 31 - 129 Aligned: 86 Modelled: 99 Confidence: 98.7% Identity: 19% Fold: Mechanosensitive channel protein MscS (YggB), transmembrane region Superfamily: Mechanosensitive channel protein MscS (YggB), transmembrane region Family: Mechanosensitive channel protein MscS (YggB), transmembrane region Phyre2 PDB 2e6z chain A Region: 144 - 195 Aligned: 49 Modelled: 52 Confidence: 85.9% Identity: 18% PDB header:transcription Chain: A: PDB Molecule:transcription elongation factor spt5; PDBTitle: solution structure of the second kow motif of human2 transcription elongation factor spt5 Phyre2 PDB 1nz9 chain A Region: 146 - 172 Aligned: 26 Modelled: 27 Confidence: 83.8% Identity: 27% Fold: SH3-like barrel Superfamily: Translation proteins SH3-like domain Family: N-utilization substance G protein NusG, C-terminal domain Phyre2 PDB 1npp chain A domain 2 Region: 146 - 172 Aligned: 26 Modelled: 27 Confidence: 83.8% Identity: 38% Fold: SH3-like barrel Superfamily: Translation proteins SH3-like domain Family: N-utilization substance G protein NusG, C-terminal domain Phyre2 PDB 2kvq chain G Region: 146 - 172 Aligned: 26 Modelled: 27 Confidence: 81.7% Identity: 27% PDB header:transcription Chain: G: PDB Molecule:transcription antitermination protein nusg; PDBTitle: solution structure of nuse:nusg-ctd complex Phyre2 PDB 2jvv chain A Region: 146 - 172 Aligned: 26 Modelled: 27 Confidence: 81.7% Identity: 27% PDB header:transcription Chain: A: PDB Molecule:transcription antitermination protein nusg; PDBTitle: solution structure of e. coli nusg carboxyterminal domain Phyre2 PDB 5xon chain W Region: 145 - 194 Aligned: 49 Modelled: 50 Confidence: 81.1% Identity: 10% PDB header:transcription/rna Chain: W: PDB Molecule:protein that forms a complex with spt4p; PDBTitle: rna polymerase ii elongation complex bound with spt4/5 and tfiis Phyre2 PDB 2mi6 chain A Region: 144 - 172 Aligned: 28 Modelled: 29 Confidence: 79.2% Identity: 29% PDB header:transcription Chain: A: PDB Molecule:transcription termination/antitermination protein nusg; PDBTitle: solution structure of the carboxy terminal domain of nusg from2 mycobacterium tuberculosis Phyre2 PDB 2zkr chain T Region: 146 - 189 Aligned: 43 Modelled: 44 Confidence: 79.1% Identity: 19% PDB header:ribosomal protein/rna Chain: T: PDB Molecule:rna expansion segment es39 part iii; PDBTitle: structure of a mammalian ribosomal 60s subunit within an 80s complex2 obtained by docking homology models of the rna and proteins into an3 8.7 a cryo-em map Phyre2 PDB 4ytl chain B Region: 147 - 195 Aligned: 48 Modelled: 49 Confidence: 77.2% Identity: 19% PDB header:transcription Chain: B: PDB Molecule:transcription elongation factor spt5; PDBTitle: structure of the kow2-kow3 domain of transcription elongation factor2 spt5. Phyre2 PDB 3p8b chain B Region: 146 - 172 Aligned: 26 Modelled: 27 Confidence: 72.0% Identity: 27% PDB header:transferase/transcription Chain: B: PDB Molecule:transcription antitermination protein nusg; PDBTitle: x-ray crystal structure of pyrococcus furiosus transcription2 elongation factor spt4/5 Phyre2 PDB 2hqh chain A domain 1 Region: 146 - 172 Aligned: 23 Modelled: 27 Confidence: 70.9% Identity: 35% Fold: SH3-like barrel Superfamily: Cap-Gly domain Family: Cap-Gly domain Phyre2 PDB 4zn3 chain A Region: 144 - 172 Aligned: 28 Modelled: 29 Confidence: 61.7% Identity: 25% PDB header:transcription Chain: A: PDB Molecule:transcription elongation factor spt5; PDBTitle: crystal structure of mjspt4:spt5 complex conformation b Phyre2 PDB 2lcl chain A Region: 144 - 196 Aligned: 52 Modelled: 53 Confidence: 57.3% Identity: 19% PDB header:transcription Chain: A: PDB Molecule:transcriptional activator rfah; PDBTitle: solution structure of rfah carboxyterminal domain Phyre2 1 c3t9nG_ 2 c2vv5D_ 3 c4hw9E_ 4 c5y4oA_ 5 d2vv5a2 6 d2vv5a1 7 d2vv5a3 8 c2e6zA_ 9 d1nz9a_ 10 d1nppa2 11 c2kvqG_ 12 c2jvvA_ 13 c5xonW_ 14 c2mi6A_ 15 c2zkrt_ 16 c4ytlB_ 17 c3p8bB_ 18 d2hqha1 19 c4zn3A_ 20 c2lclA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99