1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	L	K	N	A	V	L	L	A	C	R	A	P	S	V	H	N	S	Q	P	W	R	W	V	A	E	S	G	S	E	H	T	T	V	H	L	F	V	N	R	H	R	T	V	P	A	T	D	H	S	G	R	Q	A	I	I	S	C	G	A
Secondary structure
SS confidence
Disorder		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		V	L	D	H	L	R	I	A	M	T	A	A	H	W	Q	A	N	I	T	R	F	P	Q	P	N	Q	P	D	Q	L	A	T	V	E	F	S	P	I	D	H	V	T	A	G	Q	R	N	R	A	Q	A	I	L	Q	R	R	T	D	R	L
Secondary structure
SS confidence
Disorder																																							?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		P	F	D	S	P	M	Y	W	H	L	F	E	P	A	L	R	D	A	V	D	K	D	V	A	M	L	D	V	V	S	D	D	Q	R	T	R	L	V	V	A	S	Q	L	S	E	V	L	R	R	D	D	P	Y	Y	H	A	E	L	E	W
Secondary structure
SS confidence
Disorder				?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		W	T	S	P	F	V	L	A	H	G	V	P	P	D	T	L	A	S	D	A	E	R	L	R	V	D	L	G	R	D	F	P	V	R	S	Y	Q	N	R	R	A	E	L	A	D	D	R	S	K	V	L	V	L	S	T	P	S	D	T	R
Secondary structure
SS confidence
Disorder								?		?	?							?	?	?	?	?	?	?	?	?				?	?			?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		A	D	A	L	R	C	G	E	V	L	S	T	I	L	L	E	C	T	M	A	G	M	A	T	C	T	L	T	H	L	I	E	S	S	D	S	R	D	I	V	R	G	L	T	R	Q	R	G	E	P	Q	A	L	I	R	V	G	I	A	P
Secondary structure
SS confidence
Disorder																																																													?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.
Sequence		P	L	A	A	V	P	A	P	T	P	R	R	P	L	D	S	V	L	Q	I	R	Q	T	P	E	K	G	R	N	A	S	D	R	N	A	R	E	T	G	W	F	S	P	P
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?												?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 20%) Alpha helix ( 40%) Beta strand ( 16%)

Rank Aligned region

PDB 2ymv chain A Region: 2 - 323 Aligned: 318 Modelled: 322 Confidence: 100.0% Identity: 49% PDB header:oxidoreductase Chain: A: PDB Molecule:acg nitroreductase; PDBTitle: structure of reduced m smegmatis 5246, a homologue of m.2 tuberculosis acg Phyre2 PDB 3gr3 chain B Region: 104 - 323 Aligned: 215 Modelled: 220 Confidence: 99.9% Identity: 14% PDB header:flavoprotein Chain: B: PDB Molecule:nitroreductase; PDBTitle: crystal structure of a nitroreductase-like family protein (pnba,2 bh06130) from bartonella henselae str. houston-1 at 1.45 a resolution Phyre2 PDB 2wzv chain B Region: 106 - 322 Aligned: 210 Modelled: 217 Confidence: 99.9% Identity: 19% PDB header:oxidoreductase Chain: B: PDB Molecule:nfnb protein; PDBTitle: crystal structure of the fmn-dependent nitroreductase nfnb2 from mycobacterium smegmatis Phyre2 PDB 2isl chain B Region: 101 - 326 Aligned: 187 Modelled: 190 Confidence: 99.9% Identity: 17% PDB header:flavoprotein Chain: B: PDB Molecule:blub; PDBTitle: blub bound to reduced flavin (fmnh2) and molecular oxygen.2 (clear crystal form) Phyre2 PDB 3eo8 chain A Region: 106 - 320 Aligned: 182 Modelled: 184 Confidence: 99.9% Identity: 11% PDB header:flavoprotein Chain: A: PDB Molecule:blub-like flavoprotein; PDBTitle: crystal structure of blub-like flavoprotein (yp_001089088.1) from2 clostridium difficile 630 at 1.74 a resolution Phyre2 PDB 3gh8 chain A Region: 106 - 320 Aligned: 182 Modelled: 185 Confidence: 99.9% Identity: 16% PDB header:oxidoreductase Chain: A: PDB Molecule:iodotyrosine dehalogenase 1; PDBTitle: crystal structure of mus musculus iodotyrosine deiodinase (iyd) bound2 to fmn and di-iodotyrosine (dit) Phyre2 PDB 2i7h chain E Region: 108 - 322 Aligned: 167 Modelled: 172 Confidence: 99.9% Identity: 19% PDB header:oxidoreductase Chain: E: PDB Molecule:nitroreductase-like family protein; PDBTitle: crystal structure of the nitroreductase-like family protein from2 bacillus cereus Phyre2 PDB 3gfa chain B Region: 108 - 321 Aligned: 190 Modelled: 196 Confidence: 99.9% Identity: 12% PDB header:oxidoreductase Chain: B: PDB Molecule:putative nitroreductase; PDBTitle: crystal structure of a putative nitroreductase in complex with fmn2 (cd3205) from clostridium difficile 630 at 1.35 a resolution Phyre2 PDB 1bkj chain A Region: 107 - 326 Aligned: 158 Modelled: 164 Confidence: 99.9% Identity: 19% Fold: FMN-dependent nitroreductase-like Superfamily: FMN-dependent nitroreductase-like Family: NADH oxidase/flavin reductase Phyre2 PDB 3to0 chain A Region: 106 - 320 Aligned: 157 Modelled: 163 Confidence: 99.9% Identity: 20% PDB header:oxidoreductase Chain: A: PDB Molecule:iodotyrosine deiodinase 1; PDBTitle: crystal structure of mus musculus iodotyrosine deiodinase (iyd) c217a,2 c239a bound to fmn Phyre2 PDB 4eo3 chain A Region: 104 - 321 Aligned: 160 Modelled: 163 Confidence: 99.9% Identity: 15% PDB header:oxidoreductase Chain: A: PDB Molecule:bacterioferritin comigratory protein/nadh dehydrogenase; PDBTitle: peroxiredoxin nitroreductase fusion enzyme Phyre2 PDB 5ko8 chain B Region: 105 - 320 Aligned: 182 Modelled: 186 Confidence: 99.9% Identity: 17% PDB header:oxidoreductase Chain: B: PDB Molecule:nitroreductase; PDBTitle: crystal structure of haliscomenobacter hydrossis iodotyrosine2 deiodinase (iyd) bound to fmn and mono-iodotyrosine (i-tyr) Phyre2 PDB 3k6h chain B Region: 104 - 320 Aligned: 172 Modelled: 177 Confidence: 99.9% Identity: 21% PDB header:oxidoreductase Chain: B: PDB Molecule:nitroreductase family protein; PDBTitle: crystal structure of a nitroreductase family protein from2 agrobacterium tumefaciens str. c58 Phyre2 PDB 5hdj chain A Region: 107 - 323 Aligned: 155 Modelled: 161 Confidence: 99.9% Identity: 17% PDB header:oxidoreductase Chain: A: PDB Molecule:nfra1; PDBTitle: structure of b. megaterium nfra1 Phyre2 PDB 1f5v chain A Region: 107 - 332 Aligned: 164 Modelled: 170 Confidence: 99.9% Identity: 15% Fold: FMN-dependent nitroreductase-like Superfamily: FMN-dependent nitroreductase-like Family: NADH oxidase/flavin reductase Phyre2 PDB 4xom chain B Region: 105 - 321 Aligned: 177 Modelled: 182 Confidence: 99.9% Identity: 21% PDB header:unknown function Chain: B: PDB Molecule:coenzyme f420:l-glutamate ligase; PDBTitle: coenzyme f420:l-glutamate ligase (fbib) from mycobacterium2 tuberculosis (c-terminal domain). Phyre2 PDB 3e39 chain A Region: 105 - 326 Aligned: 162 Modelled: 166 Confidence: 99.9% Identity: 19% PDB header:oxidoreductase Chain: A: PDB Molecule:putative nitroreductase; PDBTitle: crystal structure of a putative nitroreductase in complex with fmn2 (dde_0787) from desulfovibrio desulfuricans subsp. at 1.70 a3 resolution Phyre2 PDB 3n2s chain D Region: 108 - 326 Aligned: 157 Modelled: 163 Confidence: 99.9% Identity: 18% PDB header:oxidoreductase Chain: D: PDB Molecule:nadph-dependent nitro/flavin reductase; PDBTitle: structure of nfra1 nitroreductase from b. subtilis Phyre2 PDB 1nox chain A Region: 108 - 321 Aligned: 190 Modelled: 196 Confidence: 99.9% Identity: 20% Fold: FMN-dependent nitroreductase-like Superfamily: FMN-dependent nitroreductase-like Family: NADH oxidase/flavin reductase Phyre2 PDB 3bm2 chain B Region: 107 - 313 Aligned: 160 Modelled: 165 Confidence: 99.9% Identity: 15% PDB header:oxidoreductase Chain: B: PDB Molecule:protein ydja; PDBTitle: crystal structure of a minimal nitroreductase ydja from escherichia2 coli k12 with and without fmn cofactor Phyre2 1 c2ymvA_ 2 c3gr3B_ 3 c2wzvB_ 4 c2islB_ 5 c3eo8A_ 6 c3gh8A_ 7 c2i7hE_ 8 c3gfaB_ 9 d1bkja_ 10 c3to0A_ 11 c4eo3A_ 12 c5ko8B_ 13 c3k6hB_ 14 c5hdjA_ 15 d1f5va_ 16 c4xomB_ 17 c3e39A_ 18 c3n2sD_ 19 d1noxa_ 20 c3bm2B_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83