1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	N	T	H	F	P	D	A	E	T	V	R	T	V	L	T	L	A	V	R	A	P	S	I	H	N	T	Q	P	W	R	W	R	V	C	P	T	S	L	E	L	F	S	R	P	D	M	Q	L	R	S	T	D	P	D	G	R	E	L	I
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		L	S	C	G	V	A	L	H	H	C	V	V	A	L	A	S	L	G	W	Q	A	K	V	N	R	F	P	D	P	K	D	R	C	H	L	A	T	I	G	V	Q	P	L	V	P	D	Q	A	D	V	A	L	A	A	A	I	P	R	R	R
Secondary structure
SS confidence
Disorder																																											?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		T	D	R	R	A	Y	S	C	W	P	V	P	G	G	D	I	A	L	M	A	A	R	A	A	R	G	G	V	M	L	R	Q	V	S	A	L	D	R	M	K	A	I	V	A	Q	A	V	L	D	H	V	T	D	E	E	Y	L	R	E	L
Secondary structure
SS confidence
Disorder								?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		T	I	W	S	G	R	Y	G	S	V	A	G	V	P	A	R	N	E	P	P	S	D	P	S	A	P	I	P	G	R	L	F	A	G	P	G	L	S	Q	P	S	D	V	L	P	A	D	D	G	A	A	I	L	A	L	G	T	E	T	D
Secondary structure
SS confidence
Disorder								?		?	?		?		?	?	?	?	?	?	?	?	?	?	?	?	?	?		?	?	?			?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		D	R	L	A	R	L	R	A	G	E	A	A	S	I	V	L	L	T	A	T	A	M	G	L	A	C	C	P	I	T	E	P	L	E	I	A	K	T	R	D	A	V	R	A	E	V	F	G	A	G	G	Y	P	Q	M	L	L	R	V	G
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.
Sequence		W	A	P	I	N	A	D	P	L	P	P	T	P	R	R	E	L	S	Q	V	V	E	W	P	E	E	L	L	R	Q	R	C
Secondary structure
SS confidence
Disorder					?	?	?	?	?	?	?																?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 19%) Alpha helix ( 41%) Beta strand ( 14%)

Rank Aligned region

PDB 2ymv chain A Region: 2 - 324 Aligned: 321 Modelled: 323 Confidence: 100.0% Identity: 33% PDB header:oxidoreductase Chain: A: PDB Molecule:acg nitroreductase; PDBTitle: structure of reduced m smegmatis 5246, a homologue of m.2 tuberculosis acg Phyre2 PDB 3gr3 chain B Region: 108 - 328 Aligned: 213 Modelled: 221 Confidence: 100.0% Identity: 16% PDB header:flavoprotein Chain: B: PDB Molecule:nitroreductase; PDBTitle: crystal structure of a nitroreductase-like family protein (pnba,2 bh06130) from bartonella henselae str. houston-1 at 1.45 a resolution Phyre2 PDB 2wzv chain B Region: 110 - 324 Aligned: 207 Modelled: 215 Confidence: 100.0% Identity: 18% PDB header:oxidoreductase Chain: B: PDB Molecule:nfnb protein; PDBTitle: crystal structure of the fmn-dependent nitroreductase nfnb2 from mycobacterium smegmatis Phyre2 PDB 3gh8 chain A Region: 111 - 324 Aligned: 197 Modelled: 214 Confidence: 100.0% Identity: 15% PDB header:oxidoreductase Chain: A: PDB Molecule:iodotyrosine dehalogenase 1; PDBTitle: crystal structure of mus musculus iodotyrosine deiodinase (iyd) bound2 to fmn and di-iodotyrosine (dit) Phyre2 PDB 2isl chain B Region: 101 - 323 Aligned: 204 Modelled: 218 Confidence: 100.0% Identity: 16% PDB header:flavoprotein Chain: B: PDB Molecule:blub; PDBTitle: blub bound to reduced flavin (fmnh2) and molecular oxygen.2 (clear crystal form) Phyre2 PDB 4eo3 chain A Region: 108 - 324 Aligned: 165 Modelled: 182 Confidence: 100.0% Identity: 25% PDB header:oxidoreductase Chain: A: PDB Molecule:bacterioferritin comigratory protein/nadh dehydrogenase; PDBTitle: peroxiredoxin nitroreductase fusion enzyme Phyre2 PDB 5ko8 chain B Region: 109 - 323 Aligned: 197 Modelled: 215 Confidence: 99.9% Identity: 14% PDB header:oxidoreductase Chain: B: PDB Molecule:nitroreductase; PDBTitle: crystal structure of haliscomenobacter hydrossis iodotyrosine2 deiodinase (iyd) bound to fmn and mono-iodotyrosine (i-tyr) Phyre2 PDB 3eo8 chain A Region: 110 - 325 Aligned: 208 Modelled: 215 Confidence: 99.9% Identity: 14% PDB header:flavoprotein Chain: A: PDB Molecule:blub-like flavoprotein; PDBTitle: crystal structure of blub-like flavoprotein (yp_001089088.1) from2 clostridium difficile 630 at 1.74 a resolution Phyre2 PDB 1vfr chain A Region: 111 - 324 Aligned: 197 Modelled: 214 Confidence: 99.9% Identity: 11% Fold: FMN-dependent nitroreductase-like Superfamily: FMN-dependent nitroreductase-like Family: NADH oxidase/flavin reductase Phyre2 PDB 1zch chain A domain 1 Region: 111 - 323 Aligned: 181 Modelled: 199 Confidence: 99.9% Identity: 14% Fold: FMN-dependent nitroreductase-like Superfamily: FMN-dependent nitroreductase-like Family: NADH oxidase/flavin reductase Phyre2 PDB 1nox chain A Region: 110 - 324 Aligned: 195 Modelled: 215 Confidence: 99.9% Identity: 21% Fold: FMN-dependent nitroreductase-like Superfamily: FMN-dependent nitroreductase-like Family: NADH oxidase/flavin reductase Phyre2 PDB 5hdj chain A Region: 111 - 321 Aligned: 179 Modelled: 197 Confidence: 99.9% Identity: 21% PDB header:oxidoreductase Chain: A: PDB Molecule:nfra1; PDBTitle: structure of b. megaterium nfra1 Phyre2 PDB 3k6h chain B Region: 109 - 326 Aligned: 186 Modelled: 197 Confidence: 99.9% Identity: 20% PDB header:oxidoreductase Chain: B: PDB Molecule:nitroreductase family protein; PDBTitle: crystal structure of a nitroreductase family protein from2 agrobacterium tumefaciens str. c58 Phyre2 PDB 5hei chain E Region: 111 - 325 Aligned: 182 Modelled: 200 Confidence: 99.9% Identity: 19% PDB header:oxidoreductase Chain: E: PDB Molecule:nfra2; PDBTitle: structure of b. megaterium nfra2 Phyre2 PDB 1bkj chain A Region: 111 - 321 Aligned: 157 Modelled: 175 Confidence: 99.9% Identity: 22% Fold: FMN-dependent nitroreductase-like Superfamily: FMN-dependent nitroreductase-like Family: NADH oxidase/flavin reductase Phyre2 PDB 3bem chain A Region: 108 - 323 Aligned: 198 Modelled: 216 Confidence: 99.9% Identity: 19% PDB header:oxidoreductase Chain: A: PDB Molecule:putative nad(p)h nitroreductase ydfn; PDBTitle: crystal structure of putative nitroreductase ydfn (2632848) from2 bacillus subtilis at 1.65 a resolution Phyre2 PDB 2hay chain D Region: 111 - 325 Aligned: 201 Modelled: 215 Confidence: 99.9% Identity: 15% PDB header:oxidoreductase Chain: D: PDB Molecule:putative nad(p)h-flavin oxidoreductase; PDBTitle: the crystal structure of the putative nad(p)h-flavin oxidoreductase2 from streptococcus pyogenes m1 gas Phyre2 PDB 6czp chain H Region: 110 - 324 Aligned: 198 Modelled: 215 Confidence: 99.9% Identity: 12% PDB header:oxidoreductase Chain: H: PDB Molecule:oxygen-insensitive nad(p)h nitroreductase; PDBTitle: 2.2 angstrom resolution crystal structure oxygen-insensitive nad(p)h-2 dependent nitroreductase nfsb from vibrio vulnificus in complex with3 fmn Phyre2 PDB 3n2s chain D Region: 112 - 325 Aligned: 182 Modelled: 197 Confidence: 99.9% Identity: 19% PDB header:oxidoreductase Chain: D: PDB Molecule:nadph-dependent nitro/flavin reductase; PDBTitle: structure of nfra1 nitroreductase from b. subtilis Phyre2 PDB 3ek3 chain A Region: 111 - 325 Aligned: 182 Modelled: 197 Confidence: 99.9% Identity: 15% PDB header:flavoprotein Chain: A: PDB Molecule:nitroreductase; PDBTitle: crystal structure of nitroreductase with bound fmn (yp_211706.1) from2 bacteroides fragilis nctc 9343 at 1.70 a resolution Phyre2 1 c2ymvA_ 2 c3gr3B_ 3 c2wzvB_ 4 c3gh8A_ 5 c2islB_ 6 c4eo3A_ 7 c5ko8B_ 8 c3eo8A_ 9 d1vfra_ 10 d1zcha1 11 d1noxa_ 12 c5hdjA_ 13 c3k6hB_ 14 c5heiE_ 15 d1bkja_ 16 c3bemA_ 17 c2hayD_ 18 c6czpH_ 19 c3n2sD_ 20 c3ek3A_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89