1

.

.

.

.

.

.

.

.

10

.

.

.

.

.

.

.

.

.

20

.

.

.

.

.

.

.

.

.

30

.

.

.

.

.

.

.

.

.

40

.

.

.

.

.

.

.

.

.

50

.

.

.

.

.

.

.

.

.

60

Sequence

V

S

F

V

V

L

P

P

E

I

N

S

L

R

M

F

I

G

A

G

T

A

P

M

L

A

A

A

A

A

W

D

G

L

A

E

E

L

G

T

A

A

Q

S

F

A

S

V

T

A

G

L

A

G

Q

A

W

Q

G

P

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

.

.

.

.

.

.

.

70

.

.

.

.

.

.

.

.

.

80

.

.

.

.

.

.

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.

.

90

.

.

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.

.

.

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.

.

100

.

.

.

.

.

.

.

.

.

110

.

.

.

.

.

.

.

.

.

120

Sequence

A

A

L

A

M

A

A

A

A

A

P

Y

A

G

W

L

T

A

A

A

A

Q

S

A

G

A

A

G

Q

A

R

A

V

A

S

I

F

E

A

A

Q

A

A

T

V

L

P

A

A

V

A

A

N

R

D

A

F

V

Q

L

Secondary structure

SS confidence

Disorder

Disorder confidence

.

.

.

.

.

.

.

.

.

130

.

.

.

.

.

.

.

.

.

140

.

.

.

.

.

.

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150

.

.

.

.

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.

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.

.

160

.

.

.

.

.

.

.

.

.

170

.

.

.

.

.

.

.

.

.

180

Sequence

V

M

T

N

L

F

G

Q

N

A

P

L

I

A

A

A

E

G

V

Y

E

E

M

W

A

A

D

V

A

A

M

S

G

Y

Y

S

G

A

S

A

I

A

A

Q

V

V

P

W

A

S

L

L

Q

R

F

P

G

L

G

A

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

.

.

.

.

.

.

.

190

.

.

.

.

.

.

.

.

.

200

.

.

.

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.

210

.

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220

.

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.

.

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.

.

.

.

230

.

.

.

.

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.

.

.

.

240

Sequence

G

A

T

G

A

T

G

G

E

S

V

G

T

G

A

T

G

G

E

S

V

G

T

G

G

G

E

S

V

G

T

G

G

A

T

A

S

G

G

G

V

G

Y

V

G

G

G

V

A

S

A

G

L

A

A

G

D

P

A

H

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

.

.

.

.

.

.

.

250

.

.

.

.

.

.

.

.

.

260

.

.

.

.

.

.

.

.

.

270

.

.

.

.

.

.

.

.

.

280

.

.

.

.

.

.

.

.

.

290

.

.

.

.

.

.

.

.

.

300

Sequence

G

S

V

G

Q

G

N

F

G

G

G

D

V

G

A

G

D

V

V

A

S

S

A

T

S

A

H

A

G

V

V

S

P

G

F

I

G

A

P

L

A

L

A

A

L

G

Q

M

A

R

G

G

T

N

S

A

P

G

T

A

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

.

.

.

.

.

.

.

310

.

.

.

.

.

.

.

.

.

320

.

.

.

.

.

.

.

.

.

330

.

.

.

.

.

.

.

.

.

340

.

.

.

.

.

.

.

.

.

350

.

.

.

.

.

.

.

.

.

360

Sequence

T

E

S

A

R

A

P

E

P

A

A

S

A

P

P

E

A

V

V

E

V

P

E

L

E

V

P

A

M

G

V

L

P

T

V

D

P

K

V

A

A

K

A

A

P

L

S

T

T

R

V

G

Q

S

A

G

S

G

I

P

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

.

.

.

.

.

.

.

.

.

370

.

.

.

.

.

.

.

.

.

380

.

.

.

.

.

.

.

.

.

390

.

.

.

.

.

.

.

.

.

400

.

.

.

.

.

.

.

.

.

Sequence

E

S

T

L

R

T

A

Q

G

Q

Q

A

S

E

T

S

A

A

E

E

T

A

P

S

L

R

P

E

A

A

A

G

Q

L

R

P

R

V

R

K

D

P

K

I

Q

M

R

G

G

Secondary structure

SS confidence

Disorder

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

?

Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 52%) Alpha helix ( 43%) Beta strand ( 0%)

Rank Aligned region

PDB 5xfs chain B Region: 1 - 174 Aligned: 173 Modelled: 174 Confidence: 100.0% Identity: 51% PDB header:protein transport Chain: B: PDB Molecule:ppe family protein ppe15; PDBTitle: crystal structure of pe8-ppe15 in complex with espg5 from m.2 tuberculosis Phyre2 PDB 2g38 chain B Region: 3 - 174 Aligned: 171 Modelled: 172 Confidence: 100.0% Identity: 30% PDB header:structural genomics, unknown function Chain: B: PDB Molecule:ppe family protein; PDBTitle: a pe/ppe protein complex from mycobacterium tuberculosis Phyre2 PDB 2g38 chain B domain 1 Region: 3 - 174 Aligned: 171 Modelled: 172 Confidence: 100.0% Identity: 30% Fold: Ferritin-like Superfamily: PE/PPE dimer-like Family: PPE Phyre2 PDB 4xy3 chain A Region: 4 - 175 Aligned: 155 Modelled: 172 Confidence: 100.0% Identity: 21% PDB header:protein transport Chain: A: PDB Molecule:esx-1 secretion-associated protein espb; PDBTitle: structure of esx-1 secreted protein espb Phyre2 PDB 4wj2 chain A Region: 14 - 178 Aligned: 143 Modelled: 153 Confidence: 98.7% Identity: 18% PDB header:unknown function Chain: A: PDB Molecule:antigen mtb48; PDBTitle: mycobacterial protein Phyre2 PDB 2vs0 chain B Region: 21 - 100 Aligned: 79 Modelled: 80 Confidence: 97.9% Identity: 10% PDB header:cell invasion Chain: B: PDB Molecule:virulence factor esxa; PDBTitle: structural analysis of homodimeric staphylococcal aureus2 virulence factor esxa Phyre2 PDB 4iog chain D Region: 21 - 100 Aligned: 79 Modelled: 80 Confidence: 97.8% Identity: 14% PDB header:unknown function Chain: D: PDB Molecule:secreted protein esxb; PDBTitle: the crystal structure of a secreted protein esxb (wild-type, in p212 space group) from bacillus anthracis str. sterne Phyre2 PDB 3gvm chain A Region: 21 - 100 Aligned: 79 Modelled: 80 Confidence: 97.8% Identity: 15% PDB header:viral protein Chain: A: PDB Molecule:putative uncharacterized protein sag1039; PDBTitle: structure of the homodimeric wxg-100 family protein from streptococcus2 agalactiae Phyre2 PDB 3zbh chain C Region: 21 - 100 Aligned: 79 Modelled: 80 Confidence: 97.6% Identity: 14% PDB header:unknown function Chain: C: PDB Molecule:esxa; PDBTitle: geobacillus thermodenitrificans esxa crystal form i Phyre2 PDB 1wa8 chain A domain 1 Region: 21 - 100 Aligned: 79 Modelled: 80 Confidence: 97.1% Identity: 20% Fold: Ferritin-like Superfamily: EsxAB dimer-like Family: ESAT-6 like Phyre2 PDB 4lws chain B Region: 21 - 97 Aligned: 76 Modelled: 77 Confidence: 96.4% Identity: 12% PDB header:unknown function Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: esxa : esxb (semet) hetero-dimer from thermomonospora curvata Phyre2 PDB 4lws chain A Region: 21 - 100 Aligned: 79 Modelled: 77 Confidence: 96.3% Identity: 19% PDB header:unknown function Chain: A: PDB Molecule:uncharacterized protein; PDBTitle: esxa : esxb (semet) hetero-dimer from thermomonospora curvata Phyre2 PDB 1wa8 chain B domain 1 Region: 22 - 100 Aligned: 78 Modelled: 79 Confidence: 96.1% Identity: 18% Fold: Ferritin-like Superfamily: EsxAB dimer-like Family: ESAT-6 like Phyre2 PDB 4i0x chain A Region: 24 - 97 Aligned: 73 Modelled: 74 Confidence: 95.8% Identity: 19% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:esat-6-like protein mab_3112; PDBTitle: crystal structure of the mycobacterum abscessus esxef (mab_3112-2 mab_3113) complex Phyre2 PDB 2kg7 chain B Region: 31 - 100 Aligned: 69 Modelled: 70 Confidence: 92.0% Identity: 20% PDB header:unknown function Chain: B: PDB Molecule:esat-6-like protein esxh; PDBTitle: structure and features of the complex formed by the tuberculosis2 virulence factors rv0287 and rv0288 Phyre2 PDB 4i0x chain J Region: 24 - 97 Aligned: 69 Modelled: 74 Confidence: 63.4% Identity: 23% PDB header:structural genomics, unknown function Chain: J: PDB Molecule:esat-6-like protein mab_3113; PDBTitle: crystal structure of the mycobacterum abscessus esxef (mab_3112-2 mab_3113) complex Phyre2 PDB 2iu1 chain A Region: 62 - 79 Aligned: 18 Modelled: 18 Confidence: 17.2% Identity: 33% PDB header:transcription Chain: A: PDB Molecule:eukaryotic translation initiation factor 5; PDBTitle: crystal structure of eif5 c-terminal domain Phyre2 PDB 2ful chain E Region: 62 - 79 Aligned: 18 Modelled: 18 Confidence: 16.1% Identity: 33% PDB header:translation Chain: E: PDB Molecule:eukaryotic translation initiation factor 5; PDBTitle: crystal structure of the c-terminal domain of s. cerevisiae eif5 Phyre2 PDB 5frg chain A Region: 2 - 9 Aligned: 8 Modelled: 8 Confidence: 11.9% Identity: 63% PDB header:protein binding Chain: A: PDB Molecule:formin-binding protein 1-like; PDBTitle: the nmr structure of the cdc42-interacting region of toca1 Phyre2 PDB 1paq chain A Region: 63 - 79 Aligned: 17 Modelled: 17 Confidence: 10.1% Identity: 18% PDB header:translation Chain: A: PDB Molecule:translation initiation factor eif-2b epsilon PDBTitle: crystal structure of the catalytic fragment of eukaryotic2 initiation factor 2b epsilon Phyre2 1 c5xfsB_ 2 c2g38B_ 3 d2g38b1 4 c4xy3A_ 5 c4wj2A_ 6 c2vs0B_ 7 c4iogD_ 8 c3gvmA_ 9 c3zbhC_ 10 d1wa8a1 11 c4lwsB_ 12 c4lwsA_ 13 d1wa8b1 14 c4i0xA_ 15 c2kg7B_ 16 c4i0xJ_ 17 c2iu1A_ 18 c2fulE_ 19 c5frgA_ 20 c1paqA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37