1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	G	L	E	E	Q	L	P	G	G	I	L	L	S	T	V	E	K	V	A	G	Y	V	R	K	N	S	L	W	P	A	T	F	G	L	A	C	C	A	I	E	M	M	A	T	A	G	P	R	F	D	I	A	R	F	G	M	E	R	F
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?																																						?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		S	A	T	P	R	Q	A	D	L	M	I	V	A	G	R	V	S	Q	K	M	A	P	V	L	R	Q	I	Y	D	Q	M	A	E	P	K	W	V	L	A	M	G	V	C	A	S	S	G	G	M	F	N	N	Y	A	I	V	Q	G	V	D
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		H	V	V	P	V	D	I	Y	L	P	G	C	P	P	R	P	E	M	L	L	H	A	I	L	K	L	H	E	K	I	Q	Q	M	P	L	G	I	N	R	E	R	A	I	A	E	A	E	E	A	A	L	L	A	R	P	T	I	E	M	R
Secondary structure
SS confidence
Disorder																															?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?		?	?	?	?
Disorder confidence
		.	.	.	.
Sequence		G	L	L	R
Secondary structure
SS confidence
Disorder		?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 24%) Alpha helix ( 36%) Beta strand ( 9%)

Rank Aligned region

PDB 6hum chain K Region: 3 - 183 Aligned: 175 Modelled: 181 Confidence: 100.0% Identity: 43% PDB header:proton transport Chain: K: PDB Molecule:nad(p)h-quinone oxidoreductase subunit k; PDBTitle: structure of the photosynthetic complex i from thermosynechococcus2 elongatus Phyre2 PDB 6gcs chain K Region: 8 - 154 Aligned: 146 Modelled: 147 Confidence: 100.0% Identity: 51% PDB header:oxidoreductase Chain: K: PDB Molecule:psst subunit (nukm); PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 PDB 5lc5 chain B Region: 10 - 154 Aligned: 144 Modelled: 145 Confidence: 100.0% Identity: 53% PDB header:oxidoreductase Chain: B: PDB Molecule:nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, PDBTitle: structure of mammalian respiratory complex i, class2 Phyre2 PDB 2fug chain 6 domain 1 Region: 9 - 161 Aligned: 136 Modelled: 136 Confidence: 100.0% Identity: 66% Fold: HydA/Nqo6-like Superfamily: HydA/Nqo6-like Family: Nq06-like Phyre2 PDB 6cfw chain J Region: 24 - 154 Aligned: 130 Modelled: 131 Confidence: 100.0% Identity: 36% PDB header:membrane protein Chain: J: PDB Molecule:probable membrane-bound hydrogenase subunit mbhj; PDBTitle: cryoem structure of a respiratory membrane-bound hydrogenase Phyre2 PDB 5xf9 chain G Region: 24 - 146 Aligned: 119 Modelled: 123 Confidence: 99.9% Identity: 26% PDB header:oxidoreductase Chain: G: PDB Molecule:nad-reducing hydrogenase; PDBTitle: crystal structure of nad+-reducing [nife]-hydrogenase in the air-2 oxidized state Phyre2 PDB 5odi chain E Region: 24 - 148 Aligned: 118 Modelled: 125 Confidence: 99.9% Identity: 25% PDB header:oxidoreductase Chain: E: PDB Molecule:methyl-viologen reducing hydrogenase, subunit g; PDBTitle: heterodisulfide reductase / [nife]-hydrogenase complex from2 methanothermococcus thermolithotrophicus cocrystallized with com-sh Phyre2 PDB 1frf chain S Region: 24 - 146 Aligned: 123 Modelled: 123 Confidence: 99.9% Identity: 22% Fold: HydA/Nqo6-like Superfamily: HydA/Nqo6-like Family: Nickel-iron hydrogenase, small subunit Phyre2 PDB 1e3d chain A Region: 23 - 146 Aligned: 124 Modelled: 124 Confidence: 99.9% Identity: 19% Fold: HydA/Nqo6-like Superfamily: HydA/Nqo6-like Family: Nickel-iron hydrogenase, small subunit Phyre2 PDB 6ehq chain T Region: 26 - 146 Aligned: 121 Modelled: 121 Confidence: 99.9% Identity: 21% PDB header:oxidoreductase Chain: T: PDB Molecule:hydrogenase-2 small chain; PDBTitle: e. coli hydrogenase-2 (as isolated form). Phyre2 PDB 1wui chain S domain 1 Region: 23 - 146 Aligned: 124 Modelled: 124 Confidence: 99.9% Identity: 21% Fold: HydA/Nqo6-like Superfamily: HydA/Nqo6-like Family: Nickel-iron hydrogenase, small subunit Phyre2 PDB 1h2a chain S Region: 23 - 146 Aligned: 124 Modelled: 124 Confidence: 99.9% Identity: 21% PDB header:oxidoreductase Chain: S: PDB Molecule:hydrogenase; PDBTitle: single crystals of hydrogenase from desulfovibrio vulgaris Phyre2 PDB 3zfs chain B Region: 28 - 146 Aligned: 118 Modelled: 119 Confidence: 99.9% Identity: 22% PDB header:oxidoreductase Chain: B: PDB Molecule:f420-reducing hydrogenase, subunit gamma; PDBTitle: cryo-em structure of the f420-reducing nife-hydrogenase from a2 methanogenic archaeon with bound substrate Phyre2 PDB 3rgw chain S Region: 24 - 149 Aligned: 124 Modelled: 126 Confidence: 99.9% Identity: 26% PDB header:oxidoreductase/oxidoreductase Chain: S: PDB Molecule:membrane-bound hydrogenase (nife) small subunit hoxk; PDBTitle: crystal structure at 1.5 a resolution of an h2-reduced, o2-tolerant2 hydrogenase from ralstonia eutropha unmasks a novel iron-sulfur3 cluster Phyre2 PDB 3myr chain E Region: 24 - 149 Aligned: 123 Modelled: 125 Confidence: 99.8% Identity: 27% PDB header:oxidoreductase Chain: E: PDB Molecule:hydrogenase (nife) small subunit hyda; PDBTitle: crystal structure of [nife] hydrogenase from allochromatium vinosum in2 its ni-a state Phyre2 PDB 1yq9 chain A domain 1 Region: 24 - 148 Aligned: 125 Modelled: 125 Confidence: 99.8% Identity: 19% Fold: HydA/Nqo6-like Superfamily: HydA/Nqo6-like Family: Nickel-iron hydrogenase, small subunit Phyre2 PDB 1cc1 chain S Region: 24 - 146 Aligned: 121 Modelled: 123 Confidence: 99.8% Identity: 21% Fold: HydA/Nqo6-like Superfamily: HydA/Nqo6-like Family: Nickel-iron hydrogenase, small subunit Phyre2 PDB 4gd3 chain T Region: 24 - 146 Aligned: 121 Modelled: 123 Confidence: 99.8% Identity: 25% PDB header:oxidoreductase/electron transport Chain: T: PDB Molecule:hydrogenase-1 small chain; PDBTitle: structure of e. coli hydrogenase-1 in complex with cytochrome b Phyre2 PDB 2wpn chain A Region: 28 - 148 Aligned: 119 Modelled: 121 Confidence: 99.8% Identity: 20% PDB header:oxidoreductase Chain: A: PDB Molecule:periplasmic [nifese] hydrogenase, small subunit; PDBTitle: structure of the oxidised, as-isolated nifese hydrogenase from d.2 vulgaris hildenborough Phyre2 PDB 5aa5 chain D Region: 28 - 146 Aligned: 117 Modelled: 119 Confidence: 99.4% Identity: 25% PDB header:oxidoreductase Chain: D: PDB Molecule:nife-hydrogenase small subunit, hofk; PDBTitle: actinobacterial-type nife-hydrogenase from ralstonia eutropha h16 at2 2.85 angstrom resolution Phyre2 1 c6humK_ 2 c6gcsK_ 3 c5lc5B_ 4 d2fug61 5 c6cfwJ_ 6 c5xf9G_ 7 c5odiE_ 8 d1frfs_ 9 d1e3da_ 10 c6ehqT_ 11 d1wuis1 12 c1h2aS_ 13 c3zfsB_ 14 c3rgwS_ 15 c3myrE_ 16 d1yq9a1 17 d1cc1s_ 18 c4gd3T_ 19 c2wpnA_ 20 c5aa5D_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99