1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	T	T	F	G	H	D	T	W	W	L	V	A	A	K	A	I	A	V	F	V	F	L	M	L	T	V	L	V	A	I	L	A	E	R	K	L	L	G	R	M	Q	L	R	P	G	P	N	R	V	G	P	K	G	A	L	Q	S	L	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		D	G	I	K	L	A	L	K	E	S	I	T	P	G	G	I	D	R	F	V	Y	F	V	A	P	I	I	S	V	I	P	A	F	T	A	F	A	F	I	P	F	G	P	E	V	S	V	F	G	H	R	T	P	L	Q	I	T	D	L	P
Secondary structure
SS confidence
Disorder										?		?	?	?																															?	?	?	?	?	?			?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		V	A	V	L	F	I	L	G	L	S	A	I	G	V	Y	G	I	V	L	G	G	W	A	S	G	S	T	Y	P	L	L	G	G	V	R	S	T	A	Q	V	I	S	Y	E	V	A	M	G	L	S	F	A	T	V	F	L	M	A	G	T
Secondary structure
SS confidence
Disorder																											?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		M	S	T	S	Q	I	V	A	A	Q	D	G	V	W	Y	A	F	L	L	L	P	S	F	V	I	Y	L	I	S	M	V	G	E	T	N	R	A	P	F	D	L	P	E	A	E	G	E	L	V	A	G	F	H	T	E	Y	S	S	L	K
Secondary structure
SS confidence
Disorder															?																				?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		F	A	M	F	M	L	A	E	Y	V	N	M	T	T	V	S	A	L	A	A	T	L	F	F	G	G	W	H	A	P	W	P	L	N	M	W	A	S	A	N	T	G	W	W	P	L	I	W	F	T	A	K	V	W	G	F	L	F	I	Y
Secondary structure
SS confidence
Disorder																														?	?	?		?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		F	W	L	R	A	T	L	P	R	L	R	Y	D	Q	F	M	A	L	G	W	K	L	L	I	P	V	S	L	V	W	V	M	V	A	A	I	I	R	S	L	R	N	Q	G	Y	Q	Y	W	T	P	T	L	V	F	S	S	I	V	V	A
Secondary structure
SS confidence
Disorder																																													?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410
Sequence		A	A	M	V	L	L	L	R	K	P	L	S	A	P	G	A	R	A	S	A	R	Q	R	G	D	E	G	T	S	P	E	P	A	F	P	T	P	P	L	L	A	G	A	T	K	E	N	A	G	G
Secondary structure
SS confidence
Disorder										?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?									?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 17%) Alpha helix ( 72%) Beta strand ( 0%) TM helix ( 54%) (View membrane topology prediction)

Rank Aligned region

PDB 6gcs chain 1 Region: 11 - 343 Aligned: 324 Modelled: 333 Confidence: 100.0% Identity: 36% PDB header:oxidoreductase Chain: 1: PDB Molecule:nd1 subunit (nu1m); PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 PDB 6hum chain A Region: 1 - 340 Aligned: 330 Modelled: 340 Confidence: 100.0% Identity: 37% PDB header:proton transport Chain: A: PDB Molecule:nad(p)h-quinone oxidoreductase subunit 1; PDBTitle: structure of the photosynthetic complex i from thermosynechococcus2 elongatus Phyre2 PDB 5lc5 chain H Region: 13 - 340 Aligned: 308 Modelled: 328 Confidence: 100.0% Identity: 40% PDB header:oxidoreductase Chain: H: PDB Molecule:nadh-ubiquinone oxidoreductase chain 1; PDBTitle: structure of mammalian respiratory complex i, class2 Phyre2 PDB 6cfw chain M Region: 13 - 336 Aligned: 302 Modelled: 308 Confidence: 100.0% Identity: 25% PDB header:membrane protein Chain: M: PDB Molecule:mbh13 nadh dehydrogenase subunit; PDBTitle: cryoem structure of a respiratory membrane-bound hydrogenase Phyre2 PDB 4he8 chain H Region: 44 - 363 Aligned: 290 Modelled: 302 Confidence: 100.0% Identity: 38% PDB header:oxidoreductase Chain: H: PDB Molecule:nadh-quinone oxidoreductase subunit 8; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 thermus thermophilus Phyre2 PDB 6g2j chain L Region: 123 - 227 Aligned: 100 Modelled: 105 Confidence: 91.3% Identity: 20% PDB header:oxidoreductase Chain: L: PDB Molecule:nadh-ubiquinone oxidoreductase chain 5; PDBTitle: mouse mitochondrial complex i in the active state Phyre2 PDB 6gcs chain 5 Region: 123 - 227 Aligned: 104 Modelled: 105 Confidence: 91.1% Identity: 15% PDB header:oxidoreductase Chain: 5: PDB Molecule:nd5 subunit (nu5m); PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 PDB 4hea chain T Region: 123 - 227 Aligned: 105 Modelled: 105 Confidence: 90.4% Identity: 18% PDB header:oxidoreductase Chain: T: PDB Molecule:nadh-quinone oxidoreductase subunit 12; PDBTitle: crystal structure of the entire respiratory complex i from thermus2 thermophilus Phyre2 PDB 5ldw chain M Region: 65 - 227 Aligned: 143 Modelled: 143 Confidence: 89.4% Identity: 14% PDB header:oxidoreductase Chain: M: PDB Molecule:nadh-ubiquinone oxidoreductase chain 4; PDBTitle: structure of mammalian respiratory complex i, class1 Phyre2 PDB 6gcs chain 4 Region: 123 - 227 Aligned: 101 Modelled: 105 Confidence: 88.7% Identity: 17% PDB header:oxidoreductase Chain: 4: PDB Molecule:nd4 subunit (nu4m); PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 PDB 6gcs chain 2 Region: 123 - 227 Aligned: 102 Modelled: 105 Confidence: 88.3% Identity: 7% PDB header:oxidoreductase Chain: 2: PDB Molecule:nd2 subunit (nu2m); PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 PDB 6cfw chain H Region: 123 - 263 Aligned: 140 Modelled: 141 Confidence: 88.1% Identity: 11% PDB header:membrane protein Chain: H: PDB Molecule:monovalent cation/h+ antiporter subunit d; PDBTitle: cryoem structure of a respiratory membrane-bound hydrogenase Phyre2 PDB 5ldw chain L Region: 123 - 227 Aligned: 100 Modelled: 105 Confidence: 87.7% Identity: 15% PDB header:oxidoreductase Chain: L: PDB Molecule:nadh-ubiquinone oxidoreductase chain 5; PDBTitle: structure of mammalian respiratory complex i, class1 Phyre2 PDB 4he8 chain M Region: 122 - 227 Aligned: 105 Modelled: 106 Confidence: 87.2% Identity: 11% PDB header:oxidoreductase Chain: M: PDB Molecule:nadh-quinone oxidoreductase subunit 13; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 thermus thermophilus Phyre2 PDB 6hum chain F Region: 77 - 227 Aligned: 132 Modelled: 134 Confidence: 86.4% Identity: 15% PDB header:proton transport Chain: F: PDB Molecule:nadh dehydrogenase subunit 5; PDBTitle: structure of the photosynthetic complex i from thermosynechococcus2 elongatus Phyre2 PDB 3rko chain L Region: 75 - 384 Aligned: 291 Modelled: 292 Confidence: 79.0% Identity: 13% PDB header:oxidoreductase Chain: L: PDB Molecule:nadh-quinone oxidoreductase subunit l; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 6hum chain B Region: 37 - 227 Aligned: 190 Modelled: 191 Confidence: 72.0% Identity: 12% PDB header:proton transport Chain: B: PDB Molecule:nad(p)h-quinone oxidoreductase subunit 2; PDBTitle: structure of the photosynthetic complex i from thermosynechococcus2 elongatus Phyre2 PDB 5xtd chain I Region: 122 - 227 Aligned: 95 Modelled: 106 Confidence: 50.2% Identity: 9% PDB header:oxidoreductase/electron transport Chain: I: PDB Molecule:nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit PDBTitle: cryo-em structure of human respiratory complex i Phyre2 PDB 2jpk chain A Region: 49 - 79 Aligned: 31 Modelled: 31 Confidence: 33.7% Identity: 23% PDB header:antimicrobial protein Chain: A: PDB Molecule:bacteriocin lactococcin-g subunit beta; PDBTitle: lactococcin g-b in dpc Phyre2 PDB 4p6v chain E Region: 289 - 383 Aligned: 93 Modelled: 95 Confidence: 33.4% Identity: 12% PDB header:oxidoreductase Chain: E: PDB Molecule:na(+)-translocating nadh-quinone reductase subunit e; PDBTitle: crystal structure of the na+-translocating nadh: ubiquinone2 oxidoreductase from vibrio cholerae Phyre2 1 c6gcs1_ 2 c6humA_ 3 c5lc5H_ 4 c6cfwM_ 5 c4he8H_ 6 c6g2jL_ 7 c6gcs5_ 8 c4heaT_ 9 c5ldwM_ 10 c6gcs4_ 11 c6gcs2_ 12 c6cfwH_ 13 c5ldwL_ 14 c4he8M_ 15 c6humF_ 16 c3rkoL_ 17 c6humB_ 18 c5xtdi_ 19 c2jpkA_ 20 c4p6vE_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65