1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	N	P	A	N	Y	L	Y	L	S	V	L	L	F	T	I	G	A	S	G	V	L	L	R	R	N	A	I	V	M	F	M	C	V	E	L	M	L	N	A	V	N	L	A	F	V	T	F	A	R	M	H	G	H	L	D	A	Q	M	I
Secondary structure
SS confidence
Disorder		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.
Sequence		A	F	F	T	M	V	V	A	A	C	E	V	V	V	G	L	A	I	I	M	T	I	F	R	T	R	K	S	A	S	V	D	D	A	N	L	L	K	G
Secondary structure
SS confidence
Disorder																																					?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 6%) Alpha helix ( 88%) Beta strand ( 0%) TM helix ( 61%) (View membrane topology prediction)

Rank Aligned region

PDB 3rko chain K Region: 1 - 99 Aligned: 99 Modelled: 99 Confidence: 100.0% Identity: 31% PDB header:oxidoreductase Chain: K: PDB Molecule:nadh-quinone oxidoreductase subunit k; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 6hum chain E Region: 1 - 98 Aligned: 98 Modelled: 98 Confidence: 100.0% Identity: 45% PDB header:proton transport Chain: E: PDB Molecule:nad(p)h-quinone oxidoreductase subunit 4l; PDBTitle: structure of the photosynthetic complex i from thermosynechococcus2 elongatus Phyre2 PDB 4hea chain K Region: 5 - 99 Aligned: 94 Modelled: 95 Confidence: 100.0% Identity: 53% PDB header:oxidoreductase Chain: K: PDB Molecule:nadh-quinone oxidoreductase subunit 11; PDBTitle: crystal structure of the entire respiratory complex i from thermus2 thermophilus Phyre2 PDB 6cfw chain G Region: 4 - 96 Aligned: 93 Modelled: 93 Confidence: 100.0% Identity: 19% PDB header:membrane protein Chain: G: PDB Molecule:monovalent cation/h+ antiporter subunit c; PDBTitle: cryoem structure of a respiratory membrane-bound hydrogenase Phyre2 PDB 6gcs chain L Region: 7 - 90 Aligned: 84 Modelled: 84 Confidence: 100.0% Identity: 23% PDB header:oxidoreductase Chain: L: PDB Molecule:nd4l subunit (nulm); PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 PDB 6g2j chain K Region: 7 - 98 Aligned: 92 Modelled: 92 Confidence: 99.8% Identity: 24% PDB header:oxidoreductase Chain: K: PDB Molecule:nadh-ubiquinone oxidoreductase chain 4l; PDBTitle: mouse mitochondrial complex i in the active state Phyre2 PDB 5ldw chain K Region: 7 - 97 Aligned: 91 Modelled: 91 Confidence: 99.8% Identity: 24% PDB header:oxidoreductase Chain: K: PDB Molecule:nadh-ubiquinone oxidoreductase chain 4l; PDBTitle: structure of mammalian respiratory complex i, class1 Phyre2 PDB 5lc5 chain K Region: 7 - 97 Aligned: 91 Modelled: 91 Confidence: 99.7% Identity: 24% PDB header:oxidoreductase Chain: K: PDB Molecule:nadh-ubiquinone oxidoreductase chain 4l; PDBTitle: structure of mammalian respiratory complex i, class2 Phyre2 PDB 6hum chain F Region: 4 - 96 Aligned: 90 Modelled: 93 Confidence: 89.5% Identity: 13% PDB header:proton transport Chain: F: PDB Molecule:nadh dehydrogenase subunit 5; PDBTitle: structure of the photosynthetic complex i from thermosynechococcus2 elongatus Phyre2 PDB 3rko chain L Region: 7 - 97 Aligned: 88 Modelled: 91 Confidence: 87.2% Identity: 14% PDB header:oxidoreductase Chain: L: PDB Molecule:nadh-quinone oxidoreductase subunit l; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 4hea chain T Region: 4 - 97 Aligned: 91 Modelled: 94 Confidence: 87.2% Identity: 13% PDB header:oxidoreductase Chain: T: PDB Molecule:nadh-quinone oxidoreductase subunit 12; PDBTitle: crystal structure of the entire respiratory complex i from thermus2 thermophilus Phyre2 PDB 6gcs chain 5 Region: 5 - 99 Aligned: 92 Modelled: 95 Confidence: 77.3% Identity: 12% PDB header:oxidoreductase Chain: 5: PDB Molecule:nd5 subunit (nu5m); PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 PDB 5ldw chain L Region: 4 - 97 Aligned: 91 Modelled: 94 Confidence: 73.6% Identity: 15% PDB header:oxidoreductase Chain: L: PDB Molecule:nadh-ubiquinone oxidoreductase chain 5; PDBTitle: structure of mammalian respiratory complex i, class1 Phyre2 PDB 6cfw chain H Region: 7 - 96 Aligned: 87 Modelled: 90 Confidence: 73.1% Identity: 14% PDB header:membrane protein Chain: H: PDB Molecule:monovalent cation/h+ antiporter subunit d; PDBTitle: cryoem structure of a respiratory membrane-bound hydrogenase Phyre2 PDB 6g2j chain L Region: 5 - 97 Aligned: 90 Modelled: 93 Confidence: 64.9% Identity: 14% PDB header:oxidoreductase Chain: L: PDB Molecule:nadh-ubiquinone oxidoreductase chain 5; PDBTitle: mouse mitochondrial complex i in the active state Phyre2 PDB 6gcs chain 2 Region: 8 - 98 Aligned: 88 Modelled: 91 Confidence: 62.9% Identity: 17% PDB header:oxidoreductase Chain: 2: PDB Molecule:nd2 subunit (nu2m); PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 PDB 3rko chain M Region: 4 - 90 Aligned: 87 Modelled: 87 Confidence: 59.0% Identity: 16% PDB header:oxidoreductase Chain: M: PDB Molecule:nadh-quinone oxidoreductase subunit m; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 2mkv chain A Region: 7 - 25 Aligned: 19 Modelled: 19 Confidence: 58.6% Identity: 37% PDB header:transport protein Chain: A: PDB Molecule:sodium/potassium-transporting atpase subunit gamma; PDBTitle: structure of the na,k-atpase regulatory protein fxyd2b in micelles Phyre2 PDB 2jo1 chain A Region: 7 - 25 Aligned: 19 Modelled: 19 Confidence: 58.4% Identity: 42% PDB header:hydrolase regulator Chain: A: PDB Molecule:phospholemman; PDBTitle: structure of the na,k-atpase regulatory protein fxyd1 in2 micelles Phyre2 PDB 6gcs chain 4 Region: 7 - 93 Aligned: 83 Modelled: 87 Confidence: 57.3% Identity: 14% PDB header:oxidoreductase Chain: 4: PDB Molecule:nd4 subunit (nu4m); PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 1 c3rkoK_ 2 c6humE_ 3 c4heaK_ 4 c6cfwG_ 5 c6gcsL_ 6 c6g2jK_ 7 c5ldwK_ 8 c5lc5K_ 9 c6humF_ 10 c3rkoL_ 11 c4heaT_ 12 c6gcs5_ 13 c5ldwL_ 14 c6cfwH_ 15 c6g2jL_ 16 c6gcs2_ 17 c3rkoM_ 18 c2mkvA_ 19 c2jo1A_ 20 c6gcs4_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53