1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	T	T	S	L	G	T	H	Y	T	W	L	L	V	A	L	P	L	A	G	A	A	I	L	L	F	G	G	R	R	T	D	A	W	G	H	L	L	G	C	A	A	A	L	A	A	F	G	V	G	A	M	L	L	A	D	M	L	G	R
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?																					?	?	?																													?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		D	G	L	E	R	A	I	H	Q	Q	V	F	T	W	I	P	A	G	G	L	Q	V	D	F	G	L	Q	I	D	Q	L	S	M	C	F	V	L	L	I	S	G	V	G	S	L	I	H	I	Y	S	V	G	Y	M	A	E	D	P	D	R
Secondary structure
SS confidence
Disorder		?	?	?	?					?							?	?	?																																						?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		R	R	F	F	G	Y	L	N	L	F	L	A	S	M	L	L	L	V	V	A	D	N	Y	V	L	L	Y	V	G	W	E	G	V	G	L	A	S	Y	L	L	I	G	F	W	Y	H	K	P	S	A	A	T	A	A	K	K	A	F	V	M
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		N	R	V	G	D	A	G	L	A	V	G	M	F	L	T	F	S	T	F	G	T	L	S	Y	A	G	V	F	A	G	V	P	A	A	S	R	A	V	L	T	A	I	G	L	L	M	L	L	G	A	C	A	K	S	A	Q	V	P	L	Q
Secondary structure
SS confidence
Disorder																														?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		A	W	L	G	D	A	M	E	G	P	T	P	V	S	A	L	I	H	A	A	T	M	V	T	A	G	V	Y	L	I	V	R	S	G	P	L	Y	N	L	A	P	T	A	Q	L	A	V	V	I	V	G	A	V	T	L	L	F	G	A	I
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		I	G	C	A	K	D	D	I	K	R	A	L	A	A	S	T	I	S	Q	I	G	Y	M	V	L	A	A	G	L	G	P	A	G	Y	A	F	A	I	M	H	L	L	T	H	G	F	F	K	A	G	L	F	L	G	S	G	A	V	I	H
Secondary structure
SS confidence
Disorder																														?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		A	M	H	E	E	Q	D	M	R	R	Y	G	G	L	R	A	A	L	P	V	T	F	A	T	F	G	L	A	Y	L	A	I	I	G	V	P	P	F	A	G	F	F	S	K	D	A	I	I	E	A	A	L	G	A	G	G	I	R	G	S
Secondary structure
SS confidence
Disorder													?																																									?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		L	L	G	G	A	A	L	L	G	A	G	V	T	A	F	Y	M	T	R	V	M	L	M	T	F	F	G	E	K	R	W	T	P	G	A	H	P	H	E	A	P	A	V	M	T	W	P	M	I	L	L	A	V	G	S	V	F	S	G	G
Secondary structure
SS confidence
Disorder																												?				?	?	?	?	?	?	?			?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		L	L	A	V	G	G	T	L	R	H	W	L	Q	P	V	V	G	S	H	E	E	A	T	H	A	L	P	T	W	V	A	T	T	L	A	L	G	V	V	A	V	G	I	A	V	A	Y	R	M	Y	G	T	A	P	I	P	R	V	A	P
Secondary structure
SS confidence
Disorder																						?	?																													?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	550	.	.	.	.	.	.	.	.	.	560	.	.	.	.	.	.	.	.	.	570	.	.	.	.	.	.	.	.	.	580	.	.	.	.	.	.	.	.	.	590	.	.	.	.	.	.	.	.	.	600
Sequence		V	R	V	S	A	L	T	A	A	A	R	A	D	L	Y	G	D	A	F	N	E	E	V	F	M	R	P	G	A	Q	L	T	N	A	V	V	A	V	D	D	A	G	V	D	G	S	V	N	A	L	A	T	L	V	S	Q	T	S	N	R
Secondary structure
SS confidence
Disorder		?	?	?	?								?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	610	.	.	.	.	.	.	.	.	.	620	.	.	.	.	.	.	.	.	.	630	.	.	.
Sequence		L	R	Q	M	Q	T	G	F	A	R	N	Y	A	L	S	M	L	V	G	A	V	L	V	A	A	A	L	L	V	V	Q	L	W
Secondary structure
SS confidence
Disorder																															?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 10%) Alpha helix ( 78%) Beta strand ( 0%) TM helix ( 55%) (View membrane topology prediction)

Rank Aligned region

PDB 3rko chain L Region: 8 - 630 Aligned: 603 Modelled: 623 Confidence: 100.0% Identity: 39% PDB header:oxidoreductase Chain: L: PDB Molecule:nadh-quinone oxidoreductase subunit l; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 4hea chain T Region: 10 - 627 Aligned: 600 Modelled: 618 Confidence: 100.0% Identity: 41% PDB header:oxidoreductase Chain: T: PDB Molecule:nadh-quinone oxidoreductase subunit 12; PDBTitle: crystal structure of the entire respiratory complex i from thermus2 thermophilus Phyre2 PDB 6hum chain F Region: 7 - 628 Aligned: 600 Modelled: 622 Confidence: 100.0% Identity: 41% PDB header:proton transport Chain: F: PDB Molecule:nadh dehydrogenase subunit 5; PDBTitle: structure of the photosynthetic complex i from thermosynechococcus2 elongatus Phyre2 PDB 6gcs chain 5 Region: 10 - 633 Aligned: 616 Modelled: 624 Confidence: 100.0% Identity: 31% PDB header:oxidoreductase Chain: 5: PDB Molecule:nd5 subunit (nu5m); PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 PDB 6g2j chain L Region: 8 - 630 Aligned: 599 Modelled: 623 Confidence: 100.0% Identity: 28% PDB header:oxidoreductase Chain: L: PDB Molecule:nadh-ubiquinone oxidoreductase chain 5; PDBTitle: mouse mitochondrial complex i in the active state Phyre2 PDB 5ldw chain L Region: 8 - 626 Aligned: 595 Modelled: 619 Confidence: 100.0% Identity: 30% PDB header:oxidoreductase Chain: L: PDB Molecule:nadh-ubiquinone oxidoreductase chain 5; PDBTitle: structure of mammalian respiratory complex i, class1 Phyre2 PDB 3rko chain M Region: 10 - 496 Aligned: 480 Modelled: 487 Confidence: 100.0% Identity: 19% PDB header:oxidoreductase Chain: M: PDB Molecule:nadh-quinone oxidoreductase subunit m; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 6hum chain D Region: 9 - 503 Aligned: 482 Modelled: 495 Confidence: 100.0% Identity: 19% PDB header:proton transport Chain: D: PDB Molecule:nad(p)h-quinone oxidoreductase chain 4 1; PDBTitle: structure of the photosynthetic complex i from thermosynechococcus2 elongatus Phyre2 PDB 6cfw chain H Region: 9 - 502 Aligned: 485 Modelled: 494 Confidence: 100.0% Identity: 24% PDB header:membrane protein Chain: H: PDB Molecule:monovalent cation/h+ antiporter subunit d; PDBTitle: cryoem structure of a respiratory membrane-bound hydrogenase Phyre2 PDB 6gcs chain 4 Region: 12 - 491 Aligned: 463 Modelled: 480 Confidence: 100.0% Identity: 17% PDB header:oxidoreductase Chain: 4: PDB Molecule:nd4 subunit (nu4m); PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 PDB 6hum chain B Region: 12 - 498 Aligned: 464 Modelled: 468 Confidence: 100.0% Identity: 21% PDB header:proton transport Chain: B: PDB Molecule:nad(p)h-quinone oxidoreductase subunit 2; PDBTitle: structure of the photosynthetic complex i from thermosynechococcus2 elongatus Phyre2 PDB 4he8 chain M Region: 10 - 495 Aligned: 456 Modelled: 480 Confidence: 100.0% Identity: 23% PDB header:oxidoreductase Chain: M: PDB Molecule:nadh-quinone oxidoreductase subunit 13; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 thermus thermophilus Phyre2 PDB 3rko chain N Region: 7 - 495 Aligned: 459 Modelled: 466 Confidence: 100.0% Identity: 18% PDB header:oxidoreductase Chain: N: PDB Molecule:nadh-quinone oxidoreductase subunit n; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 5ldw chain M Region: 14 - 490 Aligned: 451 Modelled: 457 Confidence: 100.0% Identity: 16% PDB header:oxidoreductase Chain: M: PDB Molecule:nadh-ubiquinone oxidoreductase chain 4; PDBTitle: structure of mammalian respiratory complex i, class1 Phyre2 PDB 4he8 chain I Region: 13 - 490 Aligned: 422 Modelled: 455 Confidence: 100.0% Identity: 24% PDB header:oxidoreductase Chain: I: PDB Molecule:nadh-quinone oxidoreductase subunit 14; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 thermus thermophilus Phyre2 PDB 6gcs chain 2 Region: 15 - 490 Aligned: 445 Modelled: 457 Confidence: 100.0% Identity: 17% PDB header:oxidoreductase Chain: 2: PDB Molecule:nd2 subunit (nu2m); PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 PDB 5xtd chain I Region: 119 - 488 Aligned: 343 Modelled: 370 Confidence: 100.0% Identity: 16% PDB header:oxidoreductase/electron transport Chain: I: PDB Molecule:nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit PDBTitle: cryo-em structure of human respiratory complex i Phyre2 PDB 6g2j chain N Region: 119 - 461 Aligned: 316 Modelled: 343 Confidence: 100.0% Identity: 19% PDB header:oxidoreductase Chain: N: PDB Molecule:nadh-ubiquinone oxidoreductase chain 2; PDBTitle: mouse mitochondrial complex i in the active state Phyre2 PDB 5ldw chain N Region: 119 - 460 Aligned: 315 Modelled: 342 Confidence: 100.0% Identity: 17% PDB header:oxidoreductase Chain: N: PDB Molecule:nadh-ubiquinone oxidoreductase chain 2; PDBTitle: structure of mammalian respiratory complex i, class1 Phyre2 PDB 6hum chain A Region: 144 - 249 Aligned: 104 Modelled: 106 Confidence: 93.4% Identity: 14% PDB header:proton transport Chain: A: PDB Molecule:nad(p)h-quinone oxidoreductase subunit 1; PDBTitle: structure of the photosynthetic complex i from thermosynechococcus2 elongatus Phyre2 1 c3rkoL_ 2 c4heaT_ 3 c6humF_ 4 c6gcs5_ 5 c6g2jL_ 6 c5ldwL_ 7 c3rkoM_ 8 c6humD_ 9 c6cfwH_ 10 c6gcs4_ 11 c6humB_ 12 c4he8M_ 13 c3rkoN_ 14 c5ldwM_ 15 c4he8I_ 16 c6gcs2_ 17 c5xtdi_ 18 c6g2jN_ 19 c5ldwN_ 20 c6humA_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57