1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	N	N	V	P	W	L	S	V	L	W	L	V	P	L	A	G	A	V	L	I	I	L	L	P	P	G	R	R	R	L	A	K	W	A	G	M	V	V	S	V	L	T	L	A	V	S	I	V	V	A	A	E	F	K	P	S	A	E	P
Secondary structure
SS confidence
Disorder		?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		Y	Q	F	V	E	K	H	S	W	I	P	A	F	G	A	G	Y	T	L	G	V	D	G	I	A	V	V	L	V	L	L	T	T	V	L	I	P	L	L	L	V	A	G	W	N	D	A	T	D	A	D	D	L	S	P	A	S	G	R	Y
Secondary structure
SS confidence
Disorder																																																	?	?	?	?	?	?	?	?		?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		P	Q	R	P	A	P	P	R	L	R	S	S	G	G	E	R	T	R	G	V	H	A	Y	V	A	L	T	L	A	I	E	S	M	V	L	M	S	V	I	A	L	D	V	L	L	F	Y	V	F	F	E	A	M	L	I	P	M	Y	F	L
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		I	G	G	F	G	Q	G	A	G	R	S	R	A	A	V	K	F	L	L	Y	N	L	F	G	G	L	I	M	L	A	A	V	I	G	L	Y	V	V	T	A	Q	Y	D	S	G	T	F	D	F	R	E	I	V	A	G	V	A	A	G	R
Secondary structure
SS confidence
Disorder																																													?										?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		Y	G	A	D	P	A	V	F	K	A	L	F	L	G	F	M	F	A	F	A	I	K	A	P	L	W	P	F	H	R	W	L	P	D	A	A	V	E	S	T	P	A	T	A	V	L	M	M	A	V	M	D	K	V	G	T	F	G	M	L
Secondary structure
SS confidence
Disorder		?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		R	Y	C	L	Q	L	F	P	D	P	S	T	Y	F	R	P	L	I	V	T	L	A	I	I	G	V	I	Y	G	A	I	V	A	I	G	Q	T	D	M	M	R	L	I	A	Y	T	S	I	S	H	F	G	F	I	I	A	G	I	F	V
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		M	T	T	Q	G	Q	S	G	S	T	L	Y	M	L	N	H	G	L	S	T	A	A	V	F	L	I	A	G	F	L	I	A	R	R	G	S	R	S	I	A	D	Y	G	G	V	Q	K	V	A	P	I	L	A	G	T	F	M	V	S	A
Secondary structure
SS confidence
Disorder		?	?																																								?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450	.	.	.	.	.	.	.	.	.	460	.	.	.	.	.	.	.	.	.	470	.	.	.	.	.	.	.	.	.	480
Sequence		M	A	T	V	S	L	P	G	L	A	P	F	I	S	E	F	L	V	L	L	G	T	F	S	R	Y	W	L	A	A	A	F	G	V	T	A	L	V	L	S	A	V	Y	M	L	W	L	Y	Q	R	V	M	T	G	P	V	A	E	G	N
Secondary structure
SS confidence
Disorder					?	?	?	?	?																																														?			?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	490	.	.	.	.	.	.	.	.	.	500	.	.	.	.	.	.	.	.	.	510	.	.	.	.	.	.	.	.	.	520	.	.	.	.	.	.	.	.	.	530	.	.	.	.	.	.	.	.	.	540
Sequence		E	R	I	G	D	L	V	G	R	E	M	I	V	V	A	P	L	I	A	L	L	L	V	L	G	V	Y	P	K	P	V	L	D	I	I	N	P	A	V	E	N	T	M	T	T	I	G	Q	H	D	P	A	P	S	V	A	H	P	V	P
Secondary structure
SS confidence
Disorder		?	?	?		?	?	?																																														?	?			?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	550	.	.	.
Sequence		A	V	G	A	S	R	T	A	E	G	P	H	P
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 15%) Alpha helix ( 71%) Beta strand ( 2%) TM helix ( 56%) (View membrane topology prediction)

Rank Aligned region

PDB 3rko chain M Region: 6 - 525 Aligned: 486 Modelled: 491 Confidence: 100.0% Identity: 33% PDB header:oxidoreductase Chain: M: PDB Molecule:nadh-quinone oxidoreductase subunit m; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 3rko chain L Region: 4 - 508 Aligned: 465 Modelled: 475 Confidence: 100.0% Identity: 21% PDB header:oxidoreductase Chain: L: PDB Molecule:nadh-quinone oxidoreductase subunit l; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 4hea chain T Region: 7 - 510 Aligned: 456 Modelled: 477 Confidence: 100.0% Identity: 22% PDB header:oxidoreductase Chain: T: PDB Molecule:nadh-quinone oxidoreductase subunit 12; PDBTitle: crystal structure of the entire respiratory complex i from thermus2 thermophilus Phyre2 PDB 6hum chain D Region: 6 - 526 Aligned: 479 Modelled: 491 Confidence: 100.0% Identity: 28% PDB header:proton transport Chain: D: PDB Molecule:nad(p)h-quinone oxidoreductase chain 4 1; PDBTitle: structure of the photosynthetic complex i from thermosynechococcus2 elongatus Phyre2 PDB 6hum chain F Region: 6 - 518 Aligned: 473 Modelled: 483 Confidence: 100.0% Identity: 22% PDB header:proton transport Chain: F: PDB Molecule:nadh dehydrogenase subunit 5; PDBTitle: structure of the photosynthetic complex i from thermosynechococcus2 elongatus Phyre2 PDB 6gcs chain 4 Region: 13 - 521 Aligned: 462 Modelled: 480 Confidence: 100.0% Identity: 29% PDB header:oxidoreductase Chain: 4: PDB Molecule:nd4 subunit (nu4m); PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 PDB 6gcs chain 5 Region: 7 - 509 Aligned: 462 Modelled: 476 Confidence: 100.0% Identity: 17% PDB header:oxidoreductase Chain: 5: PDB Molecule:nd5 subunit (nu5m); PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 PDB 4he8 chain M Region: 7 - 528 Aligned: 466 Modelled: 488 Confidence: 100.0% Identity: 32% PDB header:oxidoreductase Chain: M: PDB Molecule:nadh-quinone oxidoreductase subunit 13; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 thermus thermophilus Phyre2 PDB 6cfw chain H Region: 6 - 533 Aligned: 491 Modelled: 501 Confidence: 100.0% Identity: 23% PDB header:membrane protein Chain: H: PDB Molecule:monovalent cation/h+ antiporter subunit d; PDBTitle: cryoem structure of a respiratory membrane-bound hydrogenase Phyre2 PDB 6hum chain B Region: 3 - 524 Aligned: 472 Modelled: 484 Confidence: 100.0% Identity: 18% PDB header:proton transport Chain: B: PDB Molecule:nad(p)h-quinone oxidoreductase subunit 2; PDBTitle: structure of the photosynthetic complex i from thermosynechococcus2 elongatus Phyre2 PDB 5ldw chain L Region: 5 - 511 Aligned: 462 Modelled: 467 Confidence: 100.0% Identity: 18% PDB header:oxidoreductase Chain: L: PDB Molecule:nadh-ubiquinone oxidoreductase chain 5; PDBTitle: structure of mammalian respiratory complex i, class1 Phyre2 PDB 6g2j chain L Region: 2 - 509 Aligned: 463 Modelled: 468 Confidence: 100.0% Identity: 16% PDB header:oxidoreductase Chain: L: PDB Molecule:nadh-ubiquinone oxidoreductase chain 5; PDBTitle: mouse mitochondrial complex i in the active state Phyre2 PDB 3rko chain N Region: 1 - 520 Aligned: 463 Modelled: 466 Confidence: 100.0% Identity: 19% PDB header:oxidoreductase Chain: N: PDB Molecule:nadh-quinone oxidoreductase subunit n; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 5ldw chain M Region: 11 - 516 Aligned: 456 Modelled: 465 Confidence: 100.0% Identity: 26% PDB header:oxidoreductase Chain: M: PDB Molecule:nadh-ubiquinone oxidoreductase chain 4; PDBTitle: structure of mammalian respiratory complex i, class1 Phyre2 PDB 4he8 chain I Region: 10 - 515 Aligned: 420 Modelled: 439 Confidence: 100.0% Identity: 22% PDB header:oxidoreductase Chain: I: PDB Molecule:nadh-quinone oxidoreductase subunit 14; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 thermus thermophilus Phyre2 PDB 6gcs chain 2 Region: 11 - 516 Aligned: 445 Modelled: 473 Confidence: 100.0% Identity: 13% PDB header:oxidoreductase Chain: 2: PDB Molecule:nd2 subunit (nu2m); PDBTitle: cryo-em structure of respiratory complex i from yarrowia lipolytica Phyre2 PDB 5xtd chain I Region: 139 - 512 Aligned: 339 Modelled: 355 Confidence: 100.0% Identity: 17% PDB header:oxidoreductase/electron transport Chain: I: PDB Molecule:nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit PDBTitle: cryo-em structure of human respiratory complex i Phyre2 PDB 6g2j chain N Region: 139 - 511 Aligned: 338 Modelled: 354 Confidence: 100.0% Identity: 18% PDB header:oxidoreductase Chain: N: PDB Molecule:nadh-ubiquinone oxidoreductase chain 2; PDBTitle: mouse mitochondrial complex i in the active state Phyre2 PDB 5ldw chain N Region: 139 - 510 Aligned: 337 Modelled: 353 Confidence: 100.0% Identity: 16% PDB header:oxidoreductase Chain: N: PDB Molecule:nadh-ubiquinone oxidoreductase chain 2; PDBTitle: structure of mammalian respiratory complex i, class1 Phyre2 PDB 6cfw chain M Region: 336 - 506 Aligned: 171 Modelled: 171 Confidence: 75.2% Identity: 13% PDB header:membrane protein Chain: M: PDB Molecule:mbh13 nadh dehydrogenase subunit; PDBTitle: cryoem structure of a respiratory membrane-bound hydrogenase Phyre2 1 c3rkoM_ 2 c3rkoL_ 3 c4heaT_ 4 c6humD_ 5 c6humF_ 6 c6gcs4_ 7 c6gcs5_ 8 c4he8M_ 9 c6cfwH_ 10 c6humB_ 11 c5ldwL_ 12 c6g2jL_ 13 c3rkoN_ 14 c5ldwM_ 15 c4he8I_ 16 c6gcs2_ 17 c5xtdi_ 18 c6g2jN_ 19 c5ldwN_ 20 c6cfwM_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99