1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	P	L	V	Y	F	D	A	S	A	F	V	K	L	L	T	T	E	T	G	S	S	L	A	S	A	L	W	D	G	C	D	A	A	L	S	S	R	L	A	Y	P	E	V	R	A	A	L	A	A	A	A	R	N	H	D	L	T	E	S
Secondary structure
SS confidence
Disorder		?	?																																																							?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		E	L	A	D	A	E	R	D	W	E	D	F	W	A	A	T	R	P	V	E	L	T	A	T	V	E	Q	H	A	G	H	L	A	R	A	H	A	L	R	G	A	D	A	V	H	L	A	S	A	L	A	V	G	D	P	G	L	V	V	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.
Sequence		V	W	D	R	R	L	H	T	G	A	H	A	A	G	C	R	V	A	P	A	Q	L	D	P
Secondary structure
SS confidence
Disorder																				?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 6%) Alpha helix ( 62%) Beta strand ( 12%)

Rank Aligned region

PDB 2fe1 chain A domain 1 Region: 1 - 133 Aligned: 128 Modelled: 133 Confidence: 99.8% Identity: 29% Fold: PIN domain-like Superfamily: PIN domain-like Family: PIN domain Phyre2 PDB 2fe1 chain A Region: 1 - 133 Aligned: 128 Modelled: 133 Confidence: 99.8% Identity: 29% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:conserved hypothetical protein pae0151; PDBTitle: crystal structure of pae0151 from pyrobaculum aerophilum Phyre2 PDB 1v8p chain A Region: 4 - 134 Aligned: 117 Modelled: 131 Confidence: 99.7% Identity: 20% Fold: PIN domain-like Superfamily: PIN domain-like Family: PIN domain Phyre2 PDB 1v8p chain K Region: 4 - 134 Aligned: 117 Modelled: 126 Confidence: 99.7% Identity: 20% PDB header:structural genomics, unknown function Chain: K: PDB Molecule:hypothetical protein pae2754; PDBTitle: crystal structure of pae2754 from pyrobaculum aerophilum Phyre2 PDB 5wzf chain B Region: 4 - 139 Aligned: 122 Modelled: 136 Confidence: 99.6% Identity: 20% PDB header:hydrolase Chain: B: PDB Molecule:23s rrna-specific endonuclease vapc20; PDBTitle: crystal structure of mycobacterium tuberculosis vapc20 (rv2549c),2 sarcin-ricin loop cleaving toxin Phyre2 PDB 4xgr chain G Region: 4 - 139 Aligned: 122 Modelled: 135 Confidence: 99.6% Identity: 24% PDB header:toxin/antitoxin Chain: G: PDB Molecule:ribonuclease vapc30; PDBTitle: crystal structure of addiction module from mycobacterial species Phyre2 PDB 5x3t chain D Region: 4 - 139 Aligned: 127 Modelled: 133 Confidence: 99.4% Identity: 22% PDB header:antitoxin/toxin Chain: D: PDB Molecule:ribonuclease vapc26; PDBTitle: vapbc from mycobacterium tuberculosis Phyre2 PDB 2h1c chain A domain 1 Region: 3 - 139 Aligned: 128 Modelled: 137 Confidence: 99.4% Identity: 18% Fold: PIN domain-like Superfamily: PIN domain-like Family: PIN domain Phyre2 PDB 3zvk chain C Region: 2 - 139 Aligned: 124 Modelled: 138 Confidence: 99.3% Identity: 9% PDB header:antitoxin/toxin/dna Chain: C: PDB Molecule:toxin of toxin-antitoxin system; PDBTitle: crystal structure of vapbc2 from rickettsia felis bound to2 a dna fragment from their promoter Phyre2 PDB 6a7v chain G Region: 4 - 139 Aligned: 125 Modelled: 136 Confidence: 99.3% Identity: 14% PDB header:toxin/antitoxin Chain: G: PDB Molecule:ribonuclease vapc11; PDBTitle: crystal structure of mycobacterium tuberculosis vapbc11 toxin-2 antitoxin complex Phyre2 PDB 1y82 chain A domain 1 Region: 1 - 139 Aligned: 124 Modelled: 137 Confidence: 99.3% Identity: 15% Fold: PIN domain-like Superfamily: PIN domain-like Family: PIN domain Phyre2 PDB 6nkl chain A Region: 2 - 139 Aligned: 124 Modelled: 130 Confidence: 99.2% Identity: 12% PDB header:antitoxin Chain: A: PDB Molecule:ribonuclease vapc; PDBTitle: 2.2 a resolution structure of vapbc-1 from nontypeable haemophilus2 influenzae Phyre2 PDB 3h87 chain B Region: 4 - 144 Aligned: 126 Modelled: 141 Confidence: 99.2% Identity: 19% PDB header:toxin/antitoxin Chain: B: PDB Molecule:putative uncharacterized protein; PDBTitle: rv0301 rv0300 toxin antitoxin complex from mycobacterium tuberculosis Phyre2 PDB 1v96 chain A domain 1 Region: 4 - 139 Aligned: 119 Modelled: 136 Confidence: 99.2% Identity: 15% Fold: PIN domain-like Superfamily: PIN domain-like Family: PIN domain Phyre2 PDB 4chg chain C Region: 4 - 140 Aligned: 129 Modelled: 136 Confidence: 99.1% Identity: 17% PDB header:toxin/antitoxin Chain: C: PDB Molecule:probable ribonuclease vapc15; PDBTitle: crystal structure of vapbc15 complex from mycobacterium tuberculosis Phyre2 PDB 3tnd chain C Region: 1 - 137 Aligned: 122 Modelled: 137 Confidence: 99.1% Identity: 14% PDB header:translation, toxin Chain: C: PDB Molecule:trna(fmet)-specific endonuclease vapc; PDBTitle: crystal structure of shigella flexneri vapbc toxin-antitoxin complex Phyre2 PDB 5sv2 chain A Region: 4 - 137 Aligned: 118 Modelled: 134 Confidence: 99.1% Identity: 16% PDB header:hydrolase Chain: A: PDB Molecule:ribonuclease vapc21; PDBTitle: toxin vapc21 from mycobacterium tuberculosis Phyre2 PDB 5l6m chain C Region: 5 - 139 Aligned: 120 Modelled: 135 Confidence: 99.1% Identity: 11% PDB header:hydrolase Chain: C: PDB Molecule:ribonuclease vapc; PDBTitle: structure of caulobacter crescentus vapbc1 (vapb1deltac:vapc1 form) Phyre2 PDB 3dbo chain B Region: 4 - 140 Aligned: 118 Modelled: 137 Confidence: 99.0% Identity: 19% PDB header:toxin/antitoxin Chain: B: PDB Molecule:uncharacterized protein; PDBTitle: crystal structure of a member of the vapbc family of toxin-antitoxin2 systems, vapbc-5, from mycobacterium tuberculosis Phyre2 PDB 1w8i chain A Region: 4 - 126 Aligned: 112 Modelled: 123 Confidence: 98.6% Identity: 12% Fold: PIN domain-like Superfamily: PIN domain-like Family: PIN domain Phyre2 1 d2fe1a1 2 c2fe1A_ 3 d1v8pa_ 4 c1v8pK_ 5 c5wzfB_ 6 c4xgrG_ 7 c5x3tD_ 8 d2h1ca1 9 c3zvkC_ 10 c6a7vG_ 11 d1y82a1 12 c6nklA_ 13 c3h87B_ 14 d1v96a1 15 c4chgC_ 16 c3tndC_ 17 c5sv2A_ 18 c5l6mC_ 19 c3dboB_ 20 d1w8ia_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99