1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	V	T	R	L	S	A	S	D	A	S	F	Y	Q	L	E	N	T	A	T	P	M	Y	V	G	L	L	L	I	L	R	R	P	R	A	G	L	S	Y	E	A	L	L	E	T	V	E	Q	R	L	P	Q	I	P	R	Y	R	Q	K	V
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?																													?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		Q	E	V	K	L	G	L	A	R	P	V	W	I	D	D	R	D	F	D	I	T	Y	H	V	R	R	S	A	L	P	S	P	G	S	D	E	Q	L	H	E	L	I	A	R	L	A	A	R	P	L	D	K	S	R	P	L	W	E	M	Y
Secondary structure
SS confidence
Disorder			?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		L	V	E	G	L	E	K	N	R	I	A	L	Y	T	K	S	H	Q	A	L	I	N	G	V	T	A	L	A	I	G	H	V	I	A	D	R	T	R	R	P	P	A	F	P	E	D	I	W	V	P	E	R	D	P	G	T	T	R	L	L
Secondary structure
SS confidence
Disorder																																					?		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		L	R	A	V	G	D	W	L	V	R	P	G	A	Q	L	Q	A	V	G	S	A	V	A	G	L	V	T	N	S	G	Q	L	V	E	T	G	R	K	V	L	D	I	A	R	T	V	A	R	G	T	A	P	S	S	P	L	N	A	T	V
Secondary structure
SS confidence
Disorder											?																													?	?		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.
Sequence		S	R	N	R	R	F	T	V	A	R	A	S	L	D	D	Y	R	T	V	R	A	R	Y	D	C	D	S	T	T	W	C
Secondary structure
SS confidence
Disorder																															?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 20%) Alpha helix ( 44%) Beta strand ( 17%)

Rank Aligned region

PDB 6chj chain B Region: 2 - 269 Aligned: 231 Modelled: 234 Confidence: 100.0% Identity: 28% PDB header:transferase Chain: B: PDB Molecule:diacylglycerol o-acyltransferase; PDBTitle: wax ester synthase/diacylglycerol acyltransferase from marinobacter2 aquaeolei vt8 Phyre2 PDB 6n8e chain A Region: 2 - 269 Aligned: 239 Modelled: 249 Confidence: 100.0% Identity: 15% PDB header:hydrolase Chain: A: PDB Molecule:holo-obif1; PDBTitle: crystal structure of holo-obif1, a five domain nonribosomal peptide2 synthetase from burkholderia diffusa Phyre2 PDB 2vsq chain A Region: 2 - 269 Aligned: 241 Modelled: 251 Confidence: 100.0% Identity: 12% PDB header:ligase Chain: A: PDB Molecule:surfactin synthetase subunit 3; PDBTitle: structure of surfactin a synthetase c (srfa-c), a nonribosomal peptide2 synthetase termination module Phyre2 PDB 4zxi chain A Region: 4 - 269 Aligned: 235 Modelled: 246 Confidence: 100.0% Identity: 11% PDB header:biosynthetic protein Chain: A: PDB Molecule:tyrocidine synthetase 3; PDBTitle: crystal structure of holo-ab3403 a four domain nonribosomal peptide2 synthetase bound to amp and glycine Phyre2 PDB 4znm chain B Region: 4 - 269 Aligned: 241 Modelled: 248 Confidence: 100.0% Identity: 15% PDB header:ligase Chain: B: PDB Molecule:c-domain type ii peptide synthetase; PDBTitle: crystal structure of sgcc5 protein from streptomyces globisporus (apo2 form) Phyre2 PDB 6p1j chain A Region: 4 - 269 Aligned: 239 Modelled: 250 Confidence: 100.0% Identity: 15% PDB header:biosynthetic protein Chain: A: PDB Molecule:txo2; PDBTitle: the structure of condensation and adenylation domains of teixobactin-2 producing nonribosomal peptide synthetase txo2 serine module Phyre2 PDB 4zxj chain A Region: 4 - 269 Aligned: 240 Modelled: 248 Confidence: 100.0% Identity: 14% PDB header:biosynthetic protein Chain: A: PDB Molecule:enterobactin synthase component f; PDBTitle: crystal structure of holo-entf a nonribosomal peptide synthetase in2 the thioester-forming conformation Phyre2 PDB 6cgo chain B Region: 4 - 271 Aligned: 241 Modelled: 243 Confidence: 100.0% Identity: 16% PDB header:biosynthetic protein Chain: B: PDB Molecule:condensation domain protein; PDBTitle: molecular basis for condensation domain-mediated chain release from2 the enacyloxin polyketide synthase Phyre2 PDB 6ad3 chain A Region: 2 - 269 Aligned: 251 Modelled: 259 Confidence: 99.9% Identity: 9% PDB header:biosynthetic protein Chain: A: PDB Molecule:lovastatin nonaketide synthase moka; PDBTitle: structural characterization of the condensation domain from monacolin2 k polyketide synthase moka Phyre2 PDB 5t81 chain A Region: 2 - 269 Aligned: 243 Modelled: 252 Confidence: 99.9% Identity: 13% PDB header:biosynthetic protein Chain: A: PDB Molecule:epob; PDBTitle: rhombohedral crystal form of the epob nrps cyclization-docking2 bidomain from sorangium cellulosum Phyre2 PDB 5m6p chain B Region: 4 - 269 Aligned: 242 Modelled: 246 Confidence: 99.9% Identity: 10% PDB header:isomerase Chain: B: PDB Molecule:tyrocidine synthase 2; PDBTitle: crystal structure of the epimerization domain from module 3 of2 tyrocidine synthetase b, tycb3(e) Phyre2 PDB 5t3e chain A Region: 4 - 269 Aligned: 238 Modelled: 250 Confidence: 99.9% Identity: 11% PDB header:ligase Chain: A: PDB Molecule:bacillamide synthetase heterocyclization domain; PDBTitle: crystal structure of a nonribosomal peptide synthetase2 heterocyclization domain. Phyre2 PDB 2xhg chain A Region: 4 - 269 Aligned: 242 Modelled: 246 Confidence: 99.9% Identity: 10% PDB header:isomerase Chain: A: PDB Molecule:tyrocidine synthetase a; PDBTitle: crystal structure of the epimerization domain from the initiation2 module of tyrocidine biosynthesis Phyre2 PDB 6aef chain B Region: 4 - 269 Aligned: 238 Modelled: 250 Confidence: 99.9% Identity: 8% PDB header:transferase Chain: B: PDB Molecule:polyketide synthase associated protein papa2; PDBTitle: papa2 acyl transferase Phyre2 PDB 6m7l chain B Region: 4 - 269 Aligned: 230 Modelled: 238 Confidence: 99.9% Identity: 16% PDB header:biosynthetic protein Chain: B: PDB Molecule:putative non-ribosomal peptide synthetase; PDBTitle: complex of oxya with the x-domain from gpa biosynthesis Phyre2 PDB 4jn3 chain B Region: 4 - 269 Aligned: 243 Modelled: 244 Confidence: 99.9% Identity: 12% PDB header:biosynthetic protein Chain: B: PDB Molecule:cda peptide synthetase i; PDBTitle: crystal structures of the first condensation domain of the cda2 synthetase Phyre2 PDB 1l5a chain A Region: 5 - 269 Aligned: 232 Modelled: 243 Confidence: 99.9% Identity: 12% PDB header:biosynthetic protein Chain: A: PDB Molecule:amide synthase; PDBTitle: crystal structure of vibh, an nrps condensation enzyme Phyre2 PDB 5ja2 chain A Region: 14 - 269 Aligned: 226 Modelled: 238 Confidence: 99.9% Identity: 14% PDB header:ligase Chain: A: PDB Molecule:enterobactin synthase component f; PDBTitle: entf, a terminal nonribosomal peptide synthetase module bound to the2 non-native mbth-like protein pa2412 Phyre2 PDB 6ozv chain A Region: 4 - 269 Aligned: 234 Modelled: 247 Confidence: 99.9% Identity: 20% PDB header:biosynthetic protein Chain: A: PDB Molecule:txo1; PDBTitle: the structure of condensation and adenylation domains of teixobactin-2 producing nonribosomal peptide synthetase txo1 serine module in3 complex with amp Phyre2 PDB 4tx3 chain B Region: 4 - 269 Aligned: 242 Modelled: 252 Confidence: 99.9% Identity: 12% PDB header:oxidoreductase Chain: B: PDB Molecule:peptide synthetase, module 7; PDBTitle: complex of the x-domain and oxyb from teicoplanin biosynthesis Phyre2 1 c6chjB_ 2 c6n8eA_ 3 c2vsqA_ 4 c4zxiA_ 5 c4znmB_ 6 c6p1jA_ 7 c4zxjA_ 8 c6cgoB_ 9 c6ad3A_ 10 c5t81A_ 11 c5m6pB_ 12 c5t3eA_ 13 c2xhgA_ 14 c6aefB_ 15 c6m7lB_ 16 c4jn3B_ 17 c1l5aA_ 18 c5ja2A_ 19 c6ozvA_ 20 c4tx3B_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67