1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	T	T	Q	I	G	S	G	G	P	E	A	P	R	P	P	E	V	E	E	W	R	D	K	K	R	Y	L	W	L	M	G	L	I	A	P	T	A	L	V	V	M	L	P	L	I	W	G	M	N	Q	L	G	W	H	A	A	A	Q	V
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		P	L	W	I	G	P	I	L	L	Y	V	L	L	P	L	L	D	L	R	F	G	P	D	G	Q	N	P	P	D	E	V	T	D	R	L	E	N	D	K	Y	Y	R	Y	C	T	Y	I	Y	I	P	F	Q	Y	L	S	V	V	L	G	A
Secondary structure
SS confidence
Disorder																								?	?	?	?	?	?	?	?	?	?				?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		Y	L	F	T	A	A	N	L	S	W	L	G	F	D	G	A	L	S	W	A	G	K	L	G	V	A	L	S	V	G	V	L	G	G	V	G	I	N	T	A	H	E	M	G	H	K	K	D	S	L	E	R	W	L	S	K	I	T	L	A
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		Q	T	C	Y	G	H	F	Y	I	E	H	N	R	G	H	H	V	R	V	S	T	P	E	D	P	A	S	A	R	F	G	E	T	L	W	E	F	L	P	R	S	V	I	G	G	L	R	S	A	V	H	L	E	A	Q	R	L	R	R	L
Secondary structure
SS confidence
Disorder						?																																																							?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		G	V	S	P	W	N	P	M	T	Y	L	R	N	D	V	L	N	A	W	L	M	S	V	V	L	W	G	G	L	I	A	V	F	G	P	A	L	I	P	F	V	I	I	Q	A	V	F	G	F	S	L	L	E	A	V	N	Y	L	E	H
Secondary structure
SS confidence
Disorder				?		?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		Y	G	L	L	R	Q	K	S	A	N	G	R	Y	E	R	C	A	P	V	H	S	W	N	S	D	H	I	V	T	N	L	F	L	Y	H	L	Q	R	H	S	D	H	H	A	N	P	T	R	R	Y	Q	T	L	R	S	M	A	G	A	P
Secondary structure
SS confidence
Disorder																																																									?		?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.
Sequence		N	L	P	S	G	Y	A	S	M	I	S	L	T	Y	F	P	P	L	W	R	K	V	M	D	H	R	V	L	E	H	Y	G	G	D	I	T	R	V	N	L	H	P	R	V	R	E	K	A	L	A	R	Y	G	A	S	A
Secondary structure
SS confidence
Disorder																																					?	?	?	?	?	?	?									?	?	?	?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 13%) Alpha helix ( 66%) Beta strand ( 0%) TM helix ( 38%) (View membrane topology prediction)

Rank Aligned region

PDB 4zyo chain A Region: 83 - 357 Aligned: 247 Modelled: 275 Confidence: 98.9% Identity: 18% PDB header:oxidoreductase Chain: A: PDB Molecule:acyl-coa desaturase; PDBTitle: crystal structure of human integral membrane stearoyl-coa desaturase2 with substrate Phyre2 PDB 4ymk chain A Region: 80 - 375 Aligned: 262 Modelled: 284 Confidence: 98.7% Identity: 15% PDB header:oxidoreductase Chain: A: PDB Molecule:acyl-coa desaturase 1; PDBTitle: crystal structure of stearoyl-coenzyme a desaturase 1 Phyre2 PDB 5lnk chain N Region: 230 - 258 Aligned: 24 Modelled: 29 Confidence: 37.3% Identity: 25% PDB header:oxidoreductase Chain: N: PDB Molecule:mitochondrial complex i, nd2 subunit; PDBTitle: entire ovine respiratory complex i Phyre2 PDB 2i47 chain A Region: 157 - 165 Aligned: 9 Modelled: 9 Confidence: 34.9% Identity: 56% PDB header:hydrolase Chain: A: PDB Molecule:adam 17; PDBTitle: crystal structure of catalytic domain of tace with inhibitor Phyre2 PDB 2i47 chain A domain 1 Region: 157 - 165 Aligned: 9 Modelled: 9 Confidence: 32.9% Identity: 56% Fold: Zincin-like Superfamily: Metalloproteases ("zincins"), catalytic domain Family: TNF-alpha converting enzyme, TACE, catalytic domain Phyre2 PDB 3b8z chain B Region: 156 - 165 Aligned: 10 Modelled: 10 Confidence: 27.6% Identity: 50% PDB header:hydrolase Chain: B: PDB Molecule:protein adamts-5; PDBTitle: high resolution crystal structure of the catalytic domain of adamts-52 (aggrecanase-2) Phyre2 PDB 4a01 chain B Region: 253 - 302 Aligned: 50 Modelled: 50 Confidence: 26.2% Identity: 14% PDB header:hydrolase Chain: B: PDB Molecule:proton pyrophosphatase; PDBTitle: crystal structure of the h-translocating pyrophosphatase Phyre2 PDB 1bsw chain A Region: 157 - 165 Aligned: 9 Modelled: 9 Confidence: 25.3% Identity: 67% Fold: Zincin-like Superfamily: Metalloproteases ("zincins"), catalytic domain Family: Reprolysin-like Phyre2 PDB 1r55 chain A Region: 156 - 165 Aligned: 10 Modelled: 10 Confidence: 24.0% Identity: 50% Fold: Zincin-like Superfamily: Metalloproteases ("zincins"), catalytic domain Family: Reprolysin-like Phyre2 PDB 1atl chain A Region: 156 - 165 Aligned: 10 Modelled: 10 Confidence: 23.2% Identity: 60% Fold: Zincin-like Superfamily: Metalloproteases ("zincins"), catalytic domain Family: Reprolysin-like Phyre2 PDB 4dd8 chain B Region: 156 - 165 Aligned: 10 Modelled: 10 Confidence: 22.7% Identity: 60% PDB header:hydrolase/hydrolase inhibitor Chain: B: PDB Molecule:disintegrin and metalloproteinase domain-containing protein PDBTitle: adam-8 metalloproteinase domain with bound batimastat Phyre2 PDB 1yp1 chain A Region: 156 - 165 Aligned: 10 Modelled: 10 Confidence: 22.6% Identity: 50% PDB header:hydrolase Chain: A: PDB Molecule:fii; PDBTitle: crystal structure of a non-hemorrhagic fibrin(ogen)olytic2 metalloproteinase from venom of agkistrodon acutus Phyre2 PDB 3cqb chain B Region: 157 - 165 Aligned: 9 Modelled: 9 Confidence: 22.5% Identity: 44% PDB header:hydrolase Chain: B: PDB Molecule:probable protease htpx homolog; PDBTitle: crystal structure of heat shock protein htpx domain from vibrio2 parahaemolyticus rimd 2210633 Phyre2 PDB 2rjp chain C Region: 156 - 165 Aligned: 10 Modelled: 10 Confidence: 22.3% Identity: 40% PDB header:hydrolase Chain: C: PDB Molecule:adamts-4; PDBTitle: crystal structure of adamts4 with inhibitor bound Phyre2 PDB 1nd1 chain A Region: 157 - 165 Aligned: 9 Modelled: 9 Confidence: 22.2% Identity: 56% Fold: Zincin-like Superfamily: Metalloproteases ("zincins"), catalytic domain Family: Reprolysin-like Phyre2 PDB 2erp chain A Region: 156 - 165 Aligned: 10 Modelled: 10 Confidence: 22.0% Identity: 70% PDB header:toxin Chain: A: PDB Molecule:vascular apoptosis-inducing protein 1; PDBTitle: crystal structure of vascular apoptosis-inducing protein-1(inhibitor-2 bound form) Phyre2 PDB 1wni chain A Region: 156 - 165 Aligned: 10 Modelled: 10 Confidence: 21.1% Identity: 40% Fold: Zincin-like Superfamily: Metalloproteases ("zincins"), catalytic domain Family: Reprolysin-like Phyre2 PDB 1x1n chain A domain 1 Region: 165 - 215 Aligned: 51 Modelled: 51 Confidence: 20.9% Identity: 25% Fold: TIM beta/alpha-barrel Superfamily: (Trans)glycosidases Family: Amylase, catalytic domain Phyre2 PDB 3k7n chain A Region: 156 - 165 Aligned: 10 Modelled: 10 Confidence: 20.7% Identity: 40% PDB header:hydrolase Chain: A: PDB Molecule:k-like; PDBTitle: structures of two elapid snake venom metalloproteases with2 distinct activities highlight the disulfide patterns in the3 d domain of adamalysin family proteins Phyre2 PDB 4aig chain A Region: 157 - 165 Aligned: 9 Modelled: 9 Confidence: 20.7% Identity: 56% Fold: Zincin-like Superfamily: Metalloproteases ("zincins"), catalytic domain Family: Reprolysin-like Phyre2 1 c4zyoA_ 2 c4ymkA_ 3 c5lnkn_ 4 c2i47A_ 5 d2i47a1 6 c3b8zB_ 7 c4a01B_ 8 d1bswa_ 9 d1r55a_ 10 d1atla_ 11 c4dd8B_ 12 c1yp1A_ 13 c3cqbB_ 14 c2rjpC_ 15 d1nd1a_ 16 c2erpA_ 17 d1wnia_ 18 d1x1na1 19 c3k7nA_ 20 d4aiga_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99