1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		V	E	L	L	R	G	A	L	R	T	Y	A	W	G	S	R	T	A	I	A	E	F	T	G	R	P	V	P	A	A	H	P	E	A	E	L	W	F	G	A	H	P	G	D	P	A	W	L	Q	T	P	H	G	Q	T	S	L	L	E	A
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?																																														?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		L	V	A	D	P	E	G	Q	L	G	S	A	S	R	A	R	F	G	D	V	L	P	F	L	V	K	V	L	A	A	D	E	P	L	S	L	Q	A	H	P	S	A	E	Q	A	V	E	G	Y	L	R	E	E	R	M	G	I	P	V	S
Secondary structure
SS confidence
Disorder																																		?															?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		S	P	V	R	N	Y	R	D	T	S	H	K	P	E	L	L	V	A	L	Q	P	F	E	A	L	A	G	F	R	E	A	A	R	T	T	E	L	L	R	A	L	A	V	S	D	L	D	P	F	I	D	L	L	S	E	G	S	D	A	D
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		G	L	R	A	L	F	T	T	W	I	T	A	P	Q	P	D	I	D	V	L	V	P	A	V	L	D	G	A	I	Q	Y	V	S	S	G	A	T	E	F	G	A	E	A	K	T	V	L	E	L	G	E	R	Y	P	G	D	A	G	V	L
Secondary structure
SS confidence
Disorder																															?			?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		A	A	L	L	L	N	R	I	S	L	A	P	G	E	A	I	F	L	P	A	G	N	L	H	A	Y	V	R	G	F	G	V	E	V	M	A	N	S	D	N	V	L	R	G	G	L	T	P	K	H	V	D	V	P	E	L	L	R	V	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		D	F	A	P	T	P	K	A	R	L	R	P	P	I	R	R	E	G	L	G	L	V	F	E	T	P	T	D	E	F	A	A	T	L	L	V	L	D	G	D	H	L	G	H	E	V	D	A	S	S	G	H	D	G	P	Q	I	L	L	C
Secondary structure
SS confidence
Disorder								?																																				?	?			?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.
Sequence		T	E	G	S	A	T	V	H	G	K	C	G	S	L	T	L	Q	R	G	T	A	A	W	V	A	A	D	D	G	P	I	R	L	T	A	G	Q	P	A	K	L	F	R	A	T	V	G	L
Secondary structure
SS confidence
Disorder											?																																				?	?	?
Disorder confidence

Confidence Key High(9)                     Low (0)

? Disordered ( 8%) Alpha helix ( 28%) Beta strand ( 26%)

Rank Aligned region

PDB 3h1y chain A Region: 1 - 408 Aligned: 389 Modelled: 408 Confidence: 100.0% Identity: 38% PDB header:isomerase Chain: A: PDB Molecule:mannose-6-phosphate isomerase; PDBTitle: crystal structure of mannose 6-phosphate isomerase from2 salmonella typhimurium bound to substrate (f6p)and metal3 atom (zn) Phyre2 PDB 1pmi chain A Region: 1 - 406 Aligned: 392 Modelled: 405 Confidence: 100.0% Identity: 30% Fold: Double-stranded beta-helix Superfamily: RmlC-like cupins Family: Type I phosphomannose isomerase Phyre2 PDB 1qwr chain A Region: 3 - 406 Aligned: 294 Modelled: 303 Confidence: 100.0% Identity: 26% Fold: Double-stranded beta-helix Superfamily: RmlC-like cupins Family: Type I phosphomannose isomerase Phyre2 PDB 1zx5 chain A domain 1 Region: 1 - 408 Aligned: 292 Modelled: 308 Confidence: 100.0% Identity: 25% Fold: Double-stranded beta-helix Superfamily: RmlC-like cupins Family: Type I phosphomannose isomerase Phyre2 PDB 3rns chain A Region: 246 - 406 Aligned: 135 Modelled: 142 Confidence: 98.6% Identity: 10% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:cupin 2 conserved barrel domain protein; PDBTitle: cupin 2 conserved barrel domain protein from leptotrichia buccalis Phyre2 PDB 1sfn chain A Region: 246 - 408 Aligned: 152 Modelled: 163 Confidence: 98.6% Identity: 14% Fold: Double-stranded beta-helix Superfamily: RmlC-like cupins Family: YlbA-like Phyre2 PDB 1sef chain A Region: 246 - 408 Aligned: 152 Modelled: 163 Confidence: 98.5% Identity: 7% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:conserved hypothetical protein; PDBTitle: crystal structure of cupin domain protein ef2996 from enterococcus2 faecalis Phyre2 PDB 1sef chain A Region: 246 - 408 Aligned: 152 Modelled: 163 Confidence: 98.5% Identity: 7% Fold: Double-stranded beta-helix Superfamily: RmlC-like cupins Family: YlbA-like Phyre2 PDB 1sq4 chain A Region: 246 - 408 Aligned: 152 Modelled: 163 Confidence: 98.4% Identity: 14% Fold: Double-stranded beta-helix Superfamily: RmlC-like cupins Family: YlbA-like Phyre2 PDB 2d40 chain A domain 1 Region: 246 - 405 Aligned: 150 Modelled: 160 Confidence: 98.4% Identity: 19% Fold: Double-stranded beta-helix Superfamily: RmlC-like cupins Family: Gentisate 1,2-dioxygenase-like Phyre2 PDB 4e2s chain E Region: 246 - 408 Aligned: 150 Modelled: 163 Confidence: 98.4% Identity: 13% PDB header:hydrolase Chain: E: PDB Molecule:ureidoglycine aminohydrolase; PDBTitle: crystal structure of (s)-ureidoglycine aminohydrolase from arabidopsis2 thaliana in complex with its substrate, (s)-ureidoglycine Phyre2 PDB 2d40 chain C Region: 246 - 405 Aligned: 141 Modelled: 160 Confidence: 98.4% Identity: 22% PDB header:oxidoreductase Chain: C: PDB Molecule:putative gentisate 1,2-dioxygenase; PDBTitle: crystal structure of z3393 from escherichia coli o157:h7 Phyre2 PDB 3h7y chain A Region: 246 - 405 Aligned: 143 Modelled: 160 Confidence: 98.4% Identity: 16% PDB header:biosynthetic protein Chain: A: PDB Molecule:bacilysin biosynthesis protein bacb; PDBTitle: crystal structure of bacb, an enzyme involved in bacilysin synthesis,2 in tetragonal form Phyre2 PDB 5zbf chain A Region: 247 - 406 Aligned: 141 Modelled: 160 Confidence: 98.3% Identity: 10% PDB header:isomerase Chain: A: PDB Molecule:cupin domain protein; PDBTitle: crystal structure of 4-hydroxyphenylpyruvic acid bound aere from2 microcystis aeruginosa Phyre2 PDB 1rc6 chain A Region: 246 - 407 Aligned: 143 Modelled: 162 Confidence: 98.3% Identity: 11% Fold: Double-stranded beta-helix Superfamily: RmlC-like cupins Family: YlbA-like Phyre2 PDB 1y3t chain A domain 1 Region: 246 - 407 Aligned: 159 Modelled: 162 Confidence: 98.3% Identity: 18% Fold: Double-stranded beta-helix Superfamily: RmlC-like cupins Family: Quercetin 2,3-dioxygenase-like Phyre2 PDB 2phd chain A domain 1 Region: 246 - 405 Aligned: 148 Modelled: 160 Confidence: 98.1% Identity: 18% Fold: Double-stranded beta-helix Superfamily: RmlC-like cupins Family: Gentisate 1,2-dioxygenase-like Phyre2 PDB 3myx chain A Region: 248 - 405 Aligned: 149 Modelled: 158 Confidence: 97.9% Identity: 16% PDB header:structural genomics, unknown function Chain: A: PDB Molecule:uncharacterized protein pspto_0244; PDBTitle: crystal structure of a pspto_0244 (protein with unknown function which2 belongs to pfam duf861 family) from pseudomonas syringae pv. tomato3 str. dc3000 at 1.30 a resolution Phyre2 PDB 3bu7 chain A domain 1 Region: 247 - 405 Aligned: 150 Modelled: 154 Confidence: 97.9% Identity: 23% Fold: Double-stranded beta-helix Superfamily: RmlC-like cupins Family: Gentisate 1,2-dioxygenase-like Phyre2 PDB 3bu7 chain A Region: 247 - 405 Aligned: 150 Modelled: 154 Confidence: 97.9% Identity: 23% PDB header:oxidoreductase Chain: A: PDB Molecule:gentisate 1,2-dioxygenase; PDBTitle: crystal structure and biochemical characterization of gdosp, a2 gentisate 1,2-dioxygenase from silicibacter pomeroyi Phyre2 1 c3h1yA_ 2 d1pmia_ 3 d1qwra_ 4 d1zx5a1 5 c3rnsA_ 6 d1sfna_ 7 c1sefA_ 8 d1sefa_ 9 d1sq4a_ 10 d2d40a1 11 c4e2sE_ 12 c2d40C_ 13 c3h7yA_ 14 c5zbfA_ 15 d1rc6a_ 16 d1y3ta1 17 d2phda1 18 c3myxA_ 19 d3bu7a1 20 c3bu7A_ 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228